ESTDAB Cell Profile for ESTDAB-069 (WM-1361A)


Cell Source

Cell Type: MelanomaTissue of Origin: Unknown
Ethnic Origin: UnknownGender: Male
Medium Used: RPMI 1640, 10% FBSMorphology: Unknown
Mycoplasma: -  
Comments: None

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Cell Image (200x Magnification)

Primary Search Determinants

High Resolution HLA Typing

High Resolution HLA Typing

High Resolution Typing of HLA and Related Genes (Help on HLA High Resolution Typing)
HLA-A0101/04N, 0301
HLA-BND
HLA-C0602, 0501
HLA-DRB1040101
HLA-DRB3ND
HLA-DRB4ND
HLA-DRB5ND
HLA-DQA1ND
HLA-DQB10302, 030101
HLA-DPA1ND
HLA-DPB10401

HLA and Related Genes, Surface Expression

HLA and Related Genes, Surface Expression

FACS Data (Help on FACS Data)
Marker Initial After 48hrs IFN-Gamma Induced
Normalised Score Intensity Normalised Score Intensity
HLA-AND0ND0
HLA-A,B,CND0ND0
HLA-BND0ND0
HLA-Bw4ND0ND0
HLA-Bw6ND0ND0
HLA-CND0ND0
HLA-DPND0ND0
HLA-DQND0ND0
HLA-DRND0ND0
HLA-END0ND0
HLA-GND0ND0
MICA+4,43+2,39
MICA/B+4,47+2,33
MICB+/-1,77-1,11
B2mND0ND0

Tumour Assosciated Antigens

Tumour Assosciated Antigens

Tumour Assosciated Antigens (Help on Tumour Assosciated Antigens)
Marker Score Marker Score
BAGE++MAGE 6 
BUC1NDMart 1 ++ 
GAGE++MMP2 ND 
gp100+MTA-1 ND 
MAGE 1+MUC-1 ++ 
MAGE 10+NA17-A ND 
MAGE 12+++NY ESO 
MAGE 2+++SURVIVIN +++ 
MAGE 3++T128 ND 
MAGE 4-T17 ND 
MAGE 6+T21 ND 
Mart 1++TACC1D ND 
MMP2NDTRAG 3 +++ 
MTA-1NDTRP 1 
Chromium Release Assay (Help on Chromium Release Assay)
Marker Specificity Ratio Lysis (%)
gp100 (154-162)ND ND ND 
gp100 (209-217)ND ND ND 
Mart1 (27-25)ND ND ND 
Immunohistochemistry (Help on Immunohistochemistry)
Marker Score  
gp100ND  

Cytokine Secretions

Cytokine Secretions

Cytokine Secretion Data (Help on Cytokine Secretions)
Marker Substrate - Plastic Marker Substrate - Matrigel
Interferon Gamma Interferon Gamma 
Interleukin 10 2.21 Interleukin 10 7.30 
Interleukin 6  Interleukin 6  
Interleukin 8 1530.96 Interleukin 8 1937.30 
TGF Beta 1009.49 TGF Beta 904.72 
TNF Alpha TNF Alpha 

Secondary Search Determinants

Antigen Processing

Antigen Processing

Western Blot Data (Help on Western Blot Data)
Marker Initial After 72hrs IFN-Gamma Induced
Heavy ChainNDND
LMP2NDND
LMP7NDND
MECL1NDND
TAP1NDND
TAP2NDND

Apoptosis

Apoptosis

FACS Data (Help on FACS Data)
Marker Initial After 48hrs IFN-Gamma Induced
Normalised Score Intensity Normalised Score Intensity
B7H1+/-1,92++13,21
CD95+2,26+4,03
CD95LND0ND0
PD-1ND0ND0
TRAIL-R1-1,18-1,05
TRAIL-R2+2,68+2,91
TRAIL-R3-1,17-1,05
TRAIL-R4-1,34-1,17
RNA Protection Assay Data (Help on RNA Protection Assay)
Marker Score Marker Score
caspase-8NDRIPND
cIAP1NDTNFRp55ND
cIAP2NDTRADDND
DR3 TRAMP APO3NDTRAF1ND
FADDNDTRAF2ND
FAFNDTRAF3ND
FAPNDTRAF4ND
FasNDTRAILND
FasLNDTRPM2ND
NAIPNDXIAPND
Western Blot Data (Help on Western Blot Data)
Marker Score Marker Score
APAF1NDBcl xLND
BAXNDBcl xSND
Bcl 2NDBidND
Bcl wNDFLIPND

Cytokine Polymorphisms

Cytokine Polymorphisms

Cytokine Polymorphism Data (Help on Cytokine Polymorphisms)
Marker Position Polymorphism Polymorphism
Interferon Gamma-333  
Interferon Gamma874  
Interleukin 10-1082  
Interleukin 10-819  
Interleukin 10-592  
Interleukin 10571  
Interleukin 6-174  
TGF Beta-988  
TGF Beta-800  
TGF Beta-509  
TGF Betacodon 10  
TGF Betacodon 25  
TGF Beta652  
TGF Beta653  
TGF Beta713  
TGF Beta788  
TGF Beta869  
TGF Beta915  
TNF Alpha-1031  
TNF Alpha-863  
TNF Alpha-857  
TNF Alpha-851  
TNF Alpha-574  
TNF Alpha-376  
TNF Alpha-308  
TNF Alpha-238  
TNF Alpha488  

DNA Profile

DNA Profile

DNA Profile (Help on DNA Profiles)
Marker Score Marker Score
AmelogeninYD7S820 8, 10 
CSF1PO10, 11 FGA 22, 25 
D13S31711THO1 7, 8 
D3S135816TPOX 8, 11 
D5S81811vWA 14, 17 

Extra Cellular Matrix

Extra Cellular Matrix

Cell Adhesion (Help on Cell Adhesion)
Marker Score Marker Score
Collagen+Laminin 
Fibronectin-  
Glycosyltransferases (Help on Glycosyltransferases)
Marker Score Marker Score
FUT1+SIAT3 
FUT4+SIAT4C 
MGAT5+  
Lectins (Help on Lectins)
Mol. Weight (KDa) DSA GNA LTA NAA PHA-L SBA SNA
<30 - - - - - ND -
31-40 - 2 - - - ND -
41-50 - 1 4 1 - ND -
51-60 1 1 - 2 1 ND 2
61-70 - 1 1 - - ND -
71-80 - - 1 - 1 ND -
81-90 1 - - 1 - ND -
91-100 1 - 1 2 - ND -
101-110 1 1 1 - 1 ND -
111-120 1 1 1 - - ND -
121-130 - 1 - - 1 ND -
131-140 1 - 1 - - ND -
141-150 - - - - - ND -
151-160 - - - - - ND -
161-170 - - 1 1 - ND -
171-180 - 1 1 - - ND -
181-190 1 - - - 1 ND -
191-200 - - 1 1 - ND -
>200 1 2 2 3 - ND -

Surface Markers

Surface Markers

FACS Data (Help on FACS Data)
Marker Initial After 48hrs IFN-Gamma Induced
Normalised Score Intensity Normalised Score Intensity
CD112+3,67+3,05
CD11aND0ND0
CD11b+2ND0
CD155++14,4++13,21
CD40-1,41+3,02
CD44++30,11++39,94
CD48-1ND0
CD49d+7,9ND0
CD54+9,96++27,37
CD56-1ND0
CD57-1ND0
CD58++14,02++12,29
CD80ND0ND0
CD86ND0ND0
CD95+2,26+4,03
CD95LND0ND0
ULBP1-1,14-1,05
ULBP2+2,06-1,36
ULBP3-1,93-1,17
gp100+/-1,91+2,48
Immunohistochemistry (Help on Immunohistochemistry)
Marker Score Marker Score
P-Cadherin ND 
K-CadherinNDVE-Cadherin ND 
N-CadherinND  
gp100NDSurvivin ND 

(Epi)Genetic Analysis

(Epi)Genetic Analysis

(Epi)Genetic Analysis (Help on (Epi)Genetic Analysis)
Marker Mutation Deletion Methylation
Presence Description Presence Description Presence
APCNDNDN/AN/AND
BRAFNDNDN/AN/AN/A
CDK4NDNDN/AN/AN/A
CTNNB1NDNDN/AN/AN/A
NRASNDNDN/AN/AN/A
PTENNDNDNDNDN/A
RASSFIAN/AN/AN/AN/AND
RB1NDNDNDNDN/A
TP53NDNDN/AN/AN/A
p16NDNDNDNDND
p27N/AN/AN/AN/AND