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EBI DbfetchID Z93946; SV 1; linear; genomic DNA; STD; PHG; 4735 BP. XX AC Z93946; XX DT 11-APR-1997 (Rel. 51, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 11) XX DE Bacteriophage Dp-1 dph and pal genes and 5 open reading frames XX KW dph gene; holin; lysin; N-acetylmuramoyl-L-alanine amidase; pal gene. XX OS Streptococcus phage Dp-1 OC Viruses; dsDNA viruses, no RNA stage; Caudovirales; Siphoviridae. XX RN [1] RP 1-4375 RX DOI; 10.1046/j.1365-2958.1997.5101880.x RX PUBMED; 9379901. RA Sheehan M.M., Garcia J.L., Lopez R., Garcia P.; RT "The lytic enzyme of the pneumococcal phage Dp-1: a chimeric lysin of RT intergeneric origin"; RL Mol. Microbiol. 25(4):717-725(1997). XX RN [2] RP 1-4735 RA Garcia J.L.; RT ; RL Submitted (10-APR-1997) to the EMBL/GenBank/DDBJ databases. RL Garcia J.L., Centro de Investigaciones Biologicas (CSIC), Molecular RL Microbiology, Velazquez 144, Madrid, Madrid, Spain, 28006 XX FH Key Location/Qualifiers FH FT source 1..4735 FT /organism="Streptococcus phage Dp-1" FT /strain="Dp-1" FT /isolate="Dp-1" FT /mol_type="genomic DNA" FT /db_xref="taxon:59241" FT CDS <1..657 FT /pseudo FT /codon_start=1 FT /transl_table=11 FT /gene="orf1" FT /note="truncated ORF" FT /db_xref="PSEUDO:CAB07980.1" FT CDS 668..2266 FT /transl_table=11 FT /gene="orf2" FT /product="hypothetical protein" FT /db_xref="InterPro:IPR008577" FT /db_xref="UniProtKB/TrEMBL:O03974" FT /protein_id="CAB07981.1" FT /translation="MTKFINSYGPLHLNLYVEQVSQDVTNNSSRVSWRATVDRDGAYRT FT WTYGNISNLSVWLNGSSVHSSHPDYDTSGEEVTLASGEVTVPHNSDGTKTMSVWASFDP FT NNGVHGNITISTNYTLDSIPRSTQISSFEGNRNLGSLHTVIFNRKVNSFTHQVWYRVFG FT SDWIDLGKNHTTSVSFTPSLDLARYLPKSSSGTMDICIRTYNGTTQIGSDVYSNGWRFN FT IPDSVRPTFSGISLVDTTSAVRQILTGNNFLQIMSNIQVNFNNASGAYGSTIQAFHAEL FT VGKNQAINENGGKLGMMNFNGSATVRAWVTDTRGKQSNVQDVSINVIEYYGPSINFSVQ FT RTRQNPAIIQALRNAKVAPITVGGQQKNIMQITFSVAPLNTTNFTEDRGSASGTFTTIS FT LLTNSSANLAGNYGPDKSYIVKAKIQDRFTSTEFSATVPTESVVLNYDKDGRLGVGKVV FT EQGKAGSIDAAGDIYAGGRQVQQFQLTDNNGALNRGQYNDVGISVKQSLHGEVTNTRTT FT LRELEVNGDYFKISG" FT CDS 2278..2706 FT /transl_table=11 FT /gene="orf3" FT /product="hypothetical protein" FT /db_xref="InterPro:IPR008577" FT /db_xref="UniProtKB/TrEMBL:O03975" FT /protein_id="CAB07982.1" FT /translation="MVQSFITMSGRMFIRTANDGNSWRPNKWKEVLFKQDFEQNNWQKL FT VLQSGWNHHSTYGDAFYSKTLDGIVYLRGNVHKGLIDKEATIAVLPEGFRPKVSMYLQA FT LNNSYGNAILCIYTDGRLVVKSNVDNSWLNLDNVSFRI" FT CDS 2756..3130 FT /transl_table=11 FT /gene="orf4" FT /product="hypothetical protein" FT /db_xref="UniProtKB/TrEMBL:O03976" FT /protein_id="CAB07983.1" FT /translation="MLNLTKSRQIVAEFTIGQGAEKKLVKTTIVNIDANAVSTVSETLH FT DPDLYAANRRELRADEQKLRETRYAIEDEINSWSGGKKGEPGSNRLNKEASIYANVAKR FT HRSLDDDYYSVQRSAYCPTK" FT CDS 3048..3476 FT /transl_table=11 FT /gene="orf5" FT /product="hypothetical protein" FT /db_xref="UniProtKB/TrEMBL:O03977" FT /protein_id="CAB07984.1" FT /translation="MPMWLNDTAVLTTIITACSGVLTVLLNKLFEWKSNKAKSVLEDIS FT TTLSTLKQQVDGIDQTTVAINHQNDVIQDGTRKIQRYRLYHDLKREVITGYTTLDHFRE FT LSILFESYKNLGGNGEVEALYEKYKKLPIREEDLDETI" FT CDS 3463..3687 FT /transl_table=11 FT /gene="dph" FT /product="holin" FT /function="pore formation" FT /db_xref="GOA:O03978" FT /db_xref="UniProtKB/Swiss-Prot:O03978" FT /protein_id="CAB07985.1" FT /translation="MKLSNEQYDVAKNVVTVVVPAAIALITGLGALYQFDTTAITGTIA FT LLATFAGTVLGVSSRNYQKEQEAQNNEVE" FT CDS 3687..4577 FT /transl_table=11 FT /gene="pal" FT /standard_name="lysin" FT /product="N-acetylmuramoyl-L-alanine amidase" FT /function="cell wall hydrolytic enzyme" FT /db_xref="GOA:O03979" FT /db_xref="HSSP:1HCX" FT /db_xref="InterPro:IPR008044" FT /db_xref="UniProtKB/Swiss-Prot:O03979" FT /protein_id="CAB07986.1" FT /translation="MGVDIEKGVAWMQARKGRVSYSMDFRDGPDSYDCSSSMYYALRSA FT GASSAGWAVNTEYMHAWLIENGYELISENAPWDAKRGDIFIWGRKGASAGAGGHTGMFI FT DSDNIIHCNYAYDGISVNDHDERWYYAGQPYYYVYRLTNANAQPAEKKLGWQKDATGFW FT YARANGTYPKDEFEYIEENKSWFYFDDQGYMLAEKWLKHTDGNWYWFDRDGYMATSWKR FT IGESWYYFNRDGSMVTGWIKYYDNWYYCDATNGDMKSNAFIRYNDGWYLLLPDGRLADK FT PQFTVEPDGLITAKV" XX SQ Sequence 4735 BP; 1491 A; 953 C; 1059 G; 1232 T; 0 other; tttaaatttt ttgacaaagt taattcaaat tgtaccgctg aagcaatttt ccatgtattc 60 actcaaagtt gttcagtgtg gctcaatcat attaaaatcg aacttggtaa tatctctact 120 ccttttagtg aagcagagga agaccttaaa tatcgaattg actcaaaagc cgatcaaaag 180 ctaactaacc aacagttgac ggcactcacg gaaaaggctc aactacatga cgcagaactg 240 aaagctaagg ctacaatgga gcagttaagt aacttagaaa aggcttatga aggtagaatg 300 aaagctaatg aagaagctat caacaaatcg gaacccgacc taatcttagc ggcaagtcga 360 attgaagcta ctatccaaga acttggcggg ctacgggaac tgaagaagtt cgtcgacagt 420 tgcatgagct cttctaatca aggtctaatt atcggtaaga acgacggtag ctctaccatt 480 aaggtatcaa gtgaccgaat ttctatgttc tccgcaggga atgaagttat gtaccttacg 540 caagggttca ttcacatcga taacgggatc tttacccaat ccattcaagt cggccgattt 600 agaacggaac aatactcgtt taatccagac atgaacgtga ttcggtatgt aggataagga 660 gaataacatg acaaaattta tcaactcata cggccctctt cacttgaacc tttacgtcga 720 acaagttagt caggacgtaa cgaacaactc ctcgcgagtt agttggcgag ctactgtcga 780 ccgcgatgga gcttatcgaa cgtggactta tggaaatatt agtaaccttt ccgtatggtt 840 aaatggttca agtgttcata gcagtcaccc agactacgac acgtccggcg aagaggtaac 900 gctcgcaagt ggagaagtga ctgttcctca caatagtgac gggacaaaga caatgtccgt 960 ttgggcttcg tttgacccta ataacggcgt tcacggaaat atcactatct ctactaatta 1020 cactttagac agtattccaa ggtctacaca gatttctagt tttgagggaa atcgaaatct 1080 aggatcttta catacggtta tctttaaccg aaaagtgaac tcttttacgc atcaagtttg 1140 gtaccgagtt ttcggtagcg actggataga tttaggtaag aaccatacta ctagcgtatc 1200 ctttacgccg tcactggact tagcaaggta cttacctaaa tcaagttccg gaacaatgga 1260 catctgtatt cgaacctata acggaactac gcaaattggt agtgacgtct attcaaacgg 1320 atggaggttc aacatccccg attcagtacg tcctactttt tcgggcattt ctttagtaga 1380 cacgacttca gcggttcgac agattttaac agggaacaac ttcctccaaa tcatgtcgaa 1440 cattcaagtc aacttcaaca atgcttccgg cgcttacgga tccactatcc aagcatttca 1500 cgctgagctc gtaggtaaaa accaagctat caacgaaaac ggcggcaaat tgggtatgat 1560 gaactttaat ggctccgcta ccgtaagagc atgggttaca gacacgcgag gaaaacaatc 1620 gaacgtccaa gacgtatcta tcaatgttat agaatactat ggaccgtcta tcaatttctc 1680 cgttcaacgt actcgtcaaa atcctgcaat tatccaagct cttcgaaatg ctaaggtcgc 1740 acctataacg gtaggaggtc aacagaaaaa catcatgcaa attaccttct ccgtggcgcc 1800 gttgaacact actaatttca cagaagatag aggttcggcg tcagggacgt tcactactat 1860 ttccctactg actaactcgt ccgcgaactt agctggtaac tacgggccgg acaagtctta 1920 catagttaag gctaaaatcc aagacaggtt cacttcgact gaatttagtg ctacggtacc 1980 taccgaatca gtagttctta actatgacaa ggacggtcga cttggagttg gtaaggttgt 2040 agaacaaggg aaggcagggt caattgatgc agcaggtgat atatatgctg gaggtcgaca 2100 agttcaacag tttcagctca ctgataataa tggagcattg aacaggggtc aatataacga 2160 tgttggaata agcgtgaaac agagtttaca tggcgaagta acaaatacga ggacaaccct 2220 acgggaactc gaggtgaatg gggactattt caaaatttct ggttagatag ctggaaaatg 2280 gttcaatcct tcattacaat gtcaggaaga atgttcatca ggacagcgaa cgatggaaac 2340 agctggagac ctaacaagtg gaaagaggtt ctatttaagc aagacttcga acagaataat 2400 tggcagaaac ttgttcttca aagtgggtgg aaccatcact caacctatgg cgacgcattc 2460 tattcgaaaa ctcttgacgg catagtatat ttgagaggaa atgtgcataa aggacttatc 2520 gacaaagagg ctactattgc agtacttcct gaaggattta gaccgaaagt ttcaatgtat 2580 cttcaggctc tcaataactc atatggaaat gccattctat gtatatacac tgacggaaga 2640 cttgtggtga aatcgaatgt agataattct tggttaaatt tagacaatgt ctcatttcgt 2700 atttaatttg agctgaaatc atgttataat attttttaga aaggaggtga gaactatgtt 2760 gaaccttaca aaatcgcgcc aaattgtggc agagttcact attggacaag gagctgaaaa 2820 gaaacttgtc aaaacaacga ttgtgaacat tgatgcaaac gcagtatcaa ccgtctctga 2880 aactcttcat gacccagact tgtatgctgc gaaccgtcga gaacttcgag ctgacgagca 2940 aaaacttcgc gaaactcgtt acgcaatcga agatgaaatt aatagctgga gcgggggaaa 3000 aaagggggag cccggctcta acaggctgaa taaggaggcg tcaatctatg ccaatgtggc 3060 taaacgacac cgcagtcttg acgacgatta ttacagcgtg cagcggagtg cttactgtcc 3120 tactaaataa gttattcgaa tggaaatcga ataaagccaa gagcgtttta gaggatatct 3180 ctacaactct tagcactctt aaacagcagg tcgacgggat tgaccaaacg acagtagcaa 3240 tcaatcacca aaatgacgtc attcaagacg gaactagaaa aattcaacgt taccgtcttt 3300 atcacgactt aaaaagggaa gtgataacag gctatacaac tctcgaccat tttagagagc 3360 tctctatttt attcgaaagt tataagaacc ttggcggaaa tggtgaagtt gaagccttgt 3420 atgaaaaata caagaaatta ccaattaggg aggaagattt agatgaaact atctaacgaa 3480 caatatgacg tagcaaagaa cgtggtaacc gtagtcgttc cagcagcgat tgcactaatt 3540 acaggtcttg gagcgttgta tcaatttgac actactgcta tcacaggaac cattgcactt 3600 cttgcaactt ttgcaggtac tgttctagga gtttctagcc gaaactacca aaaggaacaa 3660 gaagctcaaa acaatgaggt ggaataatgg gagtcgatat tgaaaaaggc gttgcgtgga 3720 tgcaggcccg aaagggtcga gtatcttata gcatggactt tcgagacggt cctgatagct 3780 atgactgctc aagttctatg tactatgctc tccgctcagc cggagcttca agtgctggat 3840 gggcagtcaa tactgagtac atgcacgcat ggcttattga aaacggttat gaactaatta 3900 gtgaaaatgc tccgtgggat gctaaacgag gcgacatctt catctgggga cgcaaaggtg 3960 ctagcgcagg cgctggaggt catacaggga tgttcattga cagtgataac atcattcact 4020 gcaactacgc ctacgacgga atttccgtca acgaccacga tgagcgttgg tactatgcag 4080 gtcaacctta ctactacgtc tatcgcttga ctaacgcaaa tgctcaaccg gctgagaaga 4140 aacttggctg gcagaaagat gctactggtt tctggtacgc tcgagcaaac ggaacttatc 4200 caaaagatga gttcgagtat atcgaagaaa acaagtcttg gttctacttt gacgaccaag 4260 gctacatgct cgctgagaaa tggttgaaac atactgatgg aaattggtat tggttcgacc 4320 gtgacggata catggctacg tcatggaaac ggattggcga gtcatggtac tacttcaatc 4380 gcgatggttc aatggtaacc ggttggatta agtattacga taattggtat tattgtgatg 4440 ctaccaacgg cgacatgaaa tcgaatgcgt ttatccgtta taacgacggc tggtatctac 4500 tattaccgga cggacgtctg gcagataaac ctcaattcac cgtagagccg gacgggctca 4560 ttactgctaa agtttaaaat atagagagga ggaagctctt ttcttaatat tgtttctctt 4620 aatcccgcaa ggtttcgacc ctgcggggtt tatgtgtcgt gaattactct atttacttat 4680 tcgaagattt caattataat taaataatca acgagattca taattggagg aatga 4735 // |
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