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EBI DbfetchID Z93939; SV 1; linear; genomic DNA; STD; PRO; 5011 BP. XX AC Z93939; XX DT 10-APR-1997 (Rel. 51, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 4) XX DE B.subtilis genomic DNA fragment from yumA to yulF XX KW NADH dehydrogenase; negative regulator pai 2; thioredoxine reductase; KW yulA gene; yulB gene; yulC gene; yulD gene; yulE gene; yulF gene. XX OS Bacillus subtilis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-5011 RA Oudega B., Koningsteyn g., Ramon-De Haan M., Rodrigues L.; RT "Bacillus genome sequence project: sequence of yumA to pai; a segment of RT the bacillus genome at about 284 degrees"; RL Unpublished. XX RN [2] RP 1-1367 RA Danchin A.; RT "SUBTILIST Bacillus subtilis negative regulator of protease synthese and RT sporulation (pai) genes"; RL Unpublished. XX RN [3] RP 1-5011 RA Oudega B.; RT ; RL Submitted (09-APR-1997) to the EMBL/GenBank/DDBJ databases. RL Bauke Oudega, Molecular Microbiology, Faculty of Biology, Vrije RL Universteit, IMBW, BioCentrum Amsterdam, De Boelelaan 1087, Amsterdam, 1081 RL HV, The Netherlands XX FH Key Location/Qualifiers FH FT source 1..5011 FT /organism="Bacillus subtilis" FT /chromosome="Segment of the chromosome at 283-284 degrees" FT /map="283-284 degrees" FT /strain="168" FT /isolate="Inverse PCR fragment" FT /mol_type="genomic DNA" FT /cell_type="Bacterium" FT /note="inverse PCR fragment" FT /db_xref="taxon:1423" FT CDS complement(<1..358) FT /transl_table=11 FT /gene="yumA" FT /product="unknown" FT /db_xref="UniProtKB/TrEMBL:O05266" FT /protein_id="CAB07957.1" FT /translation="MKTATTTASHACPFCSGKGYFQLILGGSETCPSCQGDRKRQSFFF FT QPLTLFQPPRYTKNNAGGWNRLISLPVVIISIVLFFVLFFGIGFLLNMLLRMSWIMAVI FT YPIVCLFIISKEKLI" FT RBS complement(366..372) FT /gene="yumA" FT CDS complement(528..1748) FT /transl_table=11 FT /gene="yumB" FT /product="unknown" FT /product="NADH dehydrogenase" FT /note="putative" FT /db_xref="GOA:O05267" FT /db_xref="InterPro:IPR001327" FT /db_xref="UniProtKB/Swiss-Prot:O05267" FT /protein_id="CAB07953.1" FT /translation="MALNKPKIVILGAGYGGLMTVTRLTKYVGPNDADITLVNKHNYHY FT ETTWMHEASAGTLHHDRCRYQIKDVINQSRVNFVQDTVKAIKIDEKKVVLANGELQYDY FT LVIGLGAVPETFGIKGLKEYAFPIANINTSRLLREHIELQFATYNTEAEKRPDRLTIVV FT GGAGFTGIEFLGELAARVPELCKEYDVDRSLVRIICVEAAPTVLPGFDPELVDYAVHYL FT EENGVEFKIGTAVQECTPEGVRVGKKDEEPEQIKSQTVVWAAGVRGHPIVEEAGFENMR FT GRVKVNPDLRAPGHDNVFILGDSSLFMNEDTERPYPPTAQIAMQQGITVAKNLGRLIKG FT GELEEFKPDIKGTVASLGEHNAVGVVYGRKLKGTPASFMKKVIDNRSLFMIGGLGLTLK FT KGKFKFF" FT RBS complement(1753..1761) FT /gene="yumB" FT RBS 2064..2069 FT /gene="yumC" FT CDS 2080..3078 FT /transl_table=11 FT /gene="yumC" FT /product="thioredoxine reductase" FT /note="putative" FT /db_xref="GOA:O05268" FT /db_xref="InterPro:IPR000103" FT /db_xref="UniProtKB/Swiss-Prot:O05268" FT /protein_id="CAB07954.1" FT /translation="MREDTKVYDITIIGGGPVGLFTAFYGGMRQASVKIIESLPQLGGQ FT LSALYPEKYIYDVAGFPKIRAQELINNLKEQMAKFDQTICLEQAVESVEKQADGVFKLV FT QMKKPTTLKRSCITAGNGAFKPRKLELENAEQYEGKNLHYFVDDLQKFAGRRVAILGGG FT DSAVDWALMLEPIAKEVSIIHRRDKFRAHEHSVENLHASKVNVLTPFVPAELIGEDKIE FT QLVLEEVKGDRKEILEIDDLIVNYGFVSSLGPIKNWGLDIEKNSIVVKSTMETNIEGFF FT AAGDICTYEGKVNLIASGFGEAPTAVNNAKAYMDPKARVQPLHSTSLFENK" FT stem_loop 3081..3122 FT RBS 3523..3529 FT /gene="yumD" FT CDS 3536..4516 FT /transl_table=11 FT /gene="yumD" FT /product="unknown" FT /note="potential inosine or guanosine 5' monophosphate FT dehydrogenase" FT /db_xref="GOA:O05269" FT /db_xref="HSSP:1B3O" FT /db_xref="InterPro:IPR001093" FT /db_xref="UniProtKB/Swiss-Prot:O05269" FT /protein_id="CAB07955.1" FT /translation="MENVFDYEDIQLIPAKCIVNSRSECDTSVRLGGHTFKLPVVPANM FT QTIIDEKLAISLAENGYFYVMHRFEPETRIDFIKDMNARGLFSSISVGVKDEEYEFVRQ FT LAEENLTPEYVTIDIAHGHSNAVIEMIQHLKKHLPDSFVIAGNVGTPEAVRELENAGAD FT ATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAASKPIIADGGIRTHGDIAKSIRF FT GATMVMIGSLFAGHEESPGQTIEKDGKLYKEYFGSASEFPKGEKKNVEGKKMHVAHKGS FT IKDTLIEMEQDLQSSISYAGGTKLNAIRNVDYVIVKNSIFNGDKY" FT stem_loop 4524..4564 FT CDS complement(4590..4988) FT /transl_table=11 FT /gene="yumE" FT /product="negative regulator pai 2" FT /db_xref="GOA:P21341" FT /db_xref="InterPro:IPR007396" FT /db_xref="UniProtKB/Swiss-Prot:P21341" FT /protein_id="CAB07956.1" FT /translation="MFQGPHAYISSSWYSRENVPTWNYQAVHMYGKASMLEKDELAEEL FT TIMLEQKTPTQAFSVLWDKLSPKLLESELKGIVGFKIKVEDIQAAYKLSQNRNETDYMN FT VIEQLQNEENPNAKQMAELMEDKLKKQI" FT RBS complement(4995..5003) FT /gene="yumE" FT conflict 5002..5005 FT /replace="ggg" FT /citation=[2] FT conflict 4536..4539 FT /replace="gccag" FT /citation=[2] XX SQ Sequence 5011 BP; 1413 A; 1163 C; 1045 G; 1390 T; 0 other; tgatcagctt ttctttgctg ataatgaaaa ggcatacaat tggataaatc acagccataa 60 tccaagacat tctcagcagc atgtttaata agaaaccgat tccaaaaaac aagacaaaaa 120 acaaaacaat tgagatgatt acgactggca gactaatcaa cctattccac ccccctgcgt 180 tgtttttagt gtaacgcggg ggctgaaata aagtcaacgg ctggaaaaag aatgactgtc 240 ttttcctgtc cccctggcaa gacggacaag tctctgaacc gccgagaatc aattgaaaat 300 atcctttccc tgaacaaaat gggcatgcat gtgaagcggt tgtcgttgca gttttcatct 360 aatcatcacc ctttttctta attttctgat aatataccat atcaagctgt cacataagtc 420 agatcatgtg acaaatcttt tgatgtaagc ggaaacatac ttgttttcct ctcgttctct 480 ttttattttg gcgtttttta ataaagaaaa cccgcttccg cgggttttta gaaaaacttg 540 aacttgcctt ttttcaatgt cagtccgagt ccgccaatca taaacagtga gcggttatca 600 atgacttttt tcataaacga agcgggcgtg cctttcagct ttctgccata aacaacccca 660 accgcgttat gctcgccaag agaagccact gttcccttaa tgtccggctt gaattcctca 720 agctctccgc ctttaataag cctgccaagg tttttagcga ccgttatgcc ctgctgcatt 780 gcaatttgcg cagtcggcgg atacgggcgc tctgtatctt cattcataaa cagcgagctg 840 tcgccgagaa taaagacatt gtcatgtccc ggggcgcgga gatcagggtt gactttgact 900 cggccgcgca tattctcaaa accggcttcc tccacaatcg gatggccgcg gacacctgcc 960 gcccatacaa cggtttgcga tttgatctgc tctggttctt catctttttt gccgactctg 1020 actccttctg gtgtacattc ttgtacagcc gttccgatct tgaactcaac tccgttttcc 1080 tcaagataat gtacggcgta atcaacaagt tccggatcaa agcccggcaa aacggtcggc 1140 gctgcttcca cacaaatgat gcggacaagg cttctgtcaa cgtcatattc cttacagagc 1200 tccggcacgc gagcagccag ttcgccaaga aattcaattc ccgtaaatcc ggcaccgcca 1260 acaacgatcg ttaacctgtc cggccgtttt tccgcttccg tattgtaagt cgcaaactgc 1320 agctcaatat gttcccgcag caggcgcgac gtgttgatgt tggcaatcgg aaacgcatat 1380 tcttttaagc ctttaatgcc gaacgtttca ggcacggcgc cgagcccgat gactaaataa 1440 tcgtactgca attctccatt cgcaagcaca acttttttct catcaatttt gattgccttt 1500 acggtatcct gtacaaaatt cacccgtgac tggttgatta catcttttat ctgataacgg 1560 cagcggtcat gatggagcgt acccgcgctc gcttcatgca tccatgtcgt ttcataatga 1620 taattgtgtt tatttacaag cgtaatgtca gcatcattcg gcccgacata cttcgtcagc 1680 cttgtcaccg tcatcaatcc gccatatcct gcacctaaaa tcacgatttt gggcttattc 1740 aatgccatca catccacctt ctcttttttt gcttttgtta gacccataat aaaatcatca 1800 tttgtgttag cttcagctta ttggcgggat tttatgaaat atattgaaaa gttttttgtg 1860 aagtccttac ccgtagcact attatcatgt ttatccggct ggcccaagaa acattcagaa 1920 aaagatcatt tcatttggtt attatcgtag cggcctgcgg aaatattttc aagaacggat 1980 gggcacgtta gatccttacg attttttctg ctgactcgtc aatttgaacg gcaatatggt 2040 ataattaata acaattttca tttaggaggc aattttcgta tgcgagagga tacaaaggtt 2100 tatgatatta caattatagg cgggggaccg gtcggcttat tcaccgcttt ttacggcggg 2160 atgagacagg caagcgtcaa aattatcgaa agcctgcctc agctcggcgg acagcttagc 2220 gccctatacc ctgagaagta tatatatgat gtagcgggat tcccgaaaat ccgcgcgcaa 2280 gagcttatca ataacctaaa agagcaaatg gcgaaattcg accaaaccat ttgtctggag 2340 caagcggttg aatctgttga gaaacaagcg gacggcgtgt ttaagcttgt acaaatgaag 2400 aaacccacta ctctaaaacg gtcatgcata actgcaggaa acggcgcatt caaaccgaga 2460 aagctggaac ttgaaaatgc cgagcagtat gaaggcaaaa acctccatta cttcgttgat 2520 gatctgcaaa aattcgccgg cagacgcgtt gcgatccttg gcggtggaga ttccgcggtt 2580 gactgggcgc ttatgcttga gccaatcgca aaagaagtat cgatcattca ccgccgcgac 2640 aagttccgag cgcacgagca cagtgtggaa aaccttcatg cgtcgaaggt taatgtcctg 2700 acaccattcg tccctgcgga gctgatcggc gaagacaaaa tcgaacagct agtgcttgaa 2760 gaagtgaaag gcgaccgcaa agagatttta gaaattgatg acttaatcgt caactacggt 2820 ttcgtttcat ctcttggacc gatcaaaaac tggggcctgg acatcgagaa aaattccatt 2880 gtcgtgaaat caacaatgga aacaaatatc gaaggcttct ttgcagcagg tgacatttgt 2940 acatacgaag gaaaagtcaa cctgattgcc agcggcttcg gcgaggcacc gacagcagtg 3000 aacaacgcca aggcttacat ggacccgaaa gcccgcgtac agcctcttca ctcaacaagt 3060 ctttttgaaa ataaataata aaaaaaggag cttgtgtctg cgacacagct ccttttttat 3120 ttgaagatat ttatataatt caatgcagtt cctacaacca gcgcaatcac aagggctgcg 3180 actacccaga tcattgccat aataatgcgg tcctgaatct tttgggactt cggtgttttt 3240 tcttcgtttt gggccatctt attcgctcct ttcccatgtc catgccataa agaaagcctg 3300 gtgctgaacg accaagcttt gctgaacgta tatcattcgc gttttgatcc gctctctaag 3360 accattcgtt aaaactatga tacctaaact caccctgcat gacaagcttc ggcacttgca 3420 ccacacctgc cagcaaatca taatgtttga catcctaaat aaaacagagt acattatata 3480 aggcgaacat ttcatatatt atacttttaa atattcgttt ttaggagtga aaaccatgga 3540 aaacgtattt gattacgaag atattcagct aattcctgca aaatgcattg tgaatagccg 3600 ttcagaatgt gatacatccg tacgtttagg cggacataca tttaaattgc ccgtcgtccc 3660 tgccaatatg cagacgatta tagatgaaaa actagcgatt tcattagccg aaaatggcta 3720 cttttatgtg atgcaccgat ttgagcctga gacacgaatt gattttatta aagatatgaa 3780 cgcacgcggg ttattctctt ctatcagcgt gggcgtcaaa gatgaggagt atgaatttgt 3840 ccgccagctg gcagaagaaa atctgactcc tgaatacgtg acaattgaca ttgctcacgg 3900 gcactcaaat gctgtcattg aaatgatcca gcaccttaaa aaacatttgc cggacagctt 3960 tgttattgct ggcaatgtcg gaacgccgga agcggtcaga gagcttgaaa atgcgggagc 4020 ggatgccact aaagtcggca tcggaccggg aaaagtatgt attacgaaaa tcaaaacagg 4080 cttcggcaca ggcggctggc aattagcagc gctgcgctgg tgtgccaagg ctgccagcaa 4140 accgattatc gcggatggcg gcatccgcac ccatggcgac atcgcaaaat caatccgatt 4200 cggcgcaacg atggtcatga tcggttcact atttgccgga cacgaagaat cgccaggcca 4260 aacgatcgaa aaagacggaa agctttacaa ggaatacttc ggctcagcct ctgaattccc 4320 aaaaggcgaa aagaaaaatg tcgaaggcaa aaaaatgcac gtggcgcata aaggctcaat 4380 caaggataca ctgattgaaa tggagcagga tcttcaatct tctatttctt atgcaggcgg 4440 aacaaagctt aacgccatcc gcaatgtgga ttatgtgatt gtgaagaatt ccatttttaa 4500 tggggataaa tattaatcat aaaaaaacgc caagtcagcg gttctccgct tgagttggcg 4560 ttttctgcta cttcttcagc aaagtaagct tatatttgct ttttgagttt atcttccatc 4620 agttctgcca tttgttttgc atttggattt tcttcatttt gcaactgttc aatgacgttc 4680 atataatccg tttcatttcg gttctggctt aatttatatg ccgcttgaat gtcttccacc 4740 ttaatcttaa atccaacaat acctttcaat tcactttcta agagttttgg agaaagctta 4800 tcccataaca cggagaaagc ttgagttggc gttttctgct ccaacatgat tgttaattct 4860 tctgctaatt catctttctc taacatactt gcttttccat acatgtggac agcctgataa 4920 ttccacgtcg gaacattttc gcgcgaatac caggaagaag aaatataagc gtgcggtccc 4980 tgaaacatca caagcacatc ttcacaggct t 5011 // |
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