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EBI DbfetchID Z86105; SV 1; linear; genomic DNA; STD; PRO; 5513 BP. XX AC Z86105; XX DT 03-MAR-1997 (Rel. 51, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 16) XX DE A.thermophilum celA gene and manA pseudogene XX KW 1,4-beta-glucanase; beta-D-mannanase; celA gene; manA gene. XX OS Anaerocellum thermophilum OC Bacteria; Firmicutes; Clostridia; Clostridiales; Anaerocellum group; OC Anaerocellum. XX RN [1] RP 1-5435 RX PUBMED; 9493383. RA Zverlov V., Mahr S., Riedel K., Bronnenmeier K.; RT "Properties and gene structure of a bifunctional cellulolytic enzyme (CelA) RT from the extreme thermophile Anaerocellum thermophilum with separate RT glycosyl hydrolase family 9 and 48 catalytic domains"; RL Microbiology (Reading, Engl.) 144:457-465(1998). XX RN [2] RC Revised by [3] RA Zverlov V.; RT ; RL Submitted (27-FEB-1997) to the EMBL/GenBank/DDBJ databases. RL Zverlov V., Institute of Molecular Genetics, Russian Academy of Sciences, RL Kurchatov Sq., Moscow, Russia, 123182 XX RN [3] RP 1-5513 RA Zverlov V.; RT ; RL Submitted (14-JUL-1997) to the EMBL/GenBank/DDBJ databases. RL Zverlov V., Institute for Microbiology, Technical University of Munich, RL D-80290 Muenchen, Germany XX FH Key Location/Qualifiers FH FT source 1..5513 FT /organism="Anaerocellum thermophilum" FT /strain="Z-1320" FT /mol_type="genomic DNA" FT /db_xref="taxon:31899" FT CDS <1..5136 FT /codon_start=1 FT /transl_table=11 FT /gene="celA" FT /standard_name="cellulase" FT /product="1,4-beta-glucanase" FT /note="Similarity: the N-therminal part belongs to FT cellulase family E (family 9 of glycosyl hydrolases) EC FT 3.2.1.4; the C-therminal part belongs to cellulase family L FT (family 48 of glycosyl hydrolases) EC 3.2.1.91" FT /db_xref="GOA:P96311" FT /db_xref="HSSP:1G87" FT /db_xref="InterPro:IPR018221" FT /db_xref="UniProtKB/TrEMBL:P96311" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAB06786.1" FT /translation="GSFNYGEALQKAIMFYEFQMSGKLPNWVRNNWRGDSALKDGQDNG FT LDLTGGWFDAGDHVKFNLPMSYTGTMLSWAVYEYKDAFVKSGQLEHILNQIEWVNDYFV FT KCHPSKYVYYYQVGDGSKDHAWWGPAEVMQMERPSFKVTQSSPGSTVVTETAASLAAAS FT IVLKDRNPTKAATYLQHAKELYEFAEVTKSDAGYTAANGYYNSWSGFYDELSWAAVWLY FT LATNDSTYLTKAESYVQNWPKISGSNTIDYKWAHCWDDVHNGAALLLAKITGKDIYKQI FT IESHLDYWITGYNGERIKYTPKGLAWLDQWGSLRYATTTAFLAFVYSDWVGCPSTKKEI FT YRKFGESQIDYALGSAGRSFVVGFGTNPPKRPHHRTAHSSWADSQSIPSYHRHTLYGAL FT VGGPGSDDSYTDDISNYVNNEVACDYNAGFVGALAKMYQLYGGNPIPDFKAIETPTNDE FT FFVEAGINASGTNFIEIKAIVNNQSGWPAKATDKLKFRYFVDLSELIKAGYSPNQLTLS FT TNYNQGAKVSGPYVWDASKNIYYILVDFTGTLIYPGGQDKYKKEVQFRIAAPQNVQWDN FT SNDYSFQDIKGVSSGSVVKTKYIPLYDGDVKVWGDGPGTSGATPTPTATATPTPTPTVT FT PTPTPTPTSTATPTPTPTPTVTPTPTPTPTATPTSTPTPTSTPSSTPVAGGQIKVLYAN FT KETNSTTNTIRPWLKVVNTGSSSIDLSRVTIRYWYTVDGDKAQSAISDWAQIGASNVTF FT KFVKLSSSVSGADYYLEIGFKSGAGQLQAGKDTGEIQIRFNKSDWSNYNQGNDWSWMQS FT MTNYGENVKVTAYIDGVLVWGQEPSGATPTPTATPAPTVTPTPTPTPTSTPTATPTATP FT TPTPTPSSTPVAGGQIKVLYANKETNSTTNTIRPWLKVVNTGSSSIDLSRVTIRYWYTV FT DGDKAQSAISDWAQIGASNVTFKFVKLSSSVSGADYYLEIGFKSGAGQLQAGKDTGEIQ FT IRFNKSDWSNYNQGNDWSWMQSMTNYGENVKVTAYIDGVLVWGQEPSGATPTPTATPAP FT TVTPTPTPTPTSTPTATPTATPTPTPTPSSTPSVVGEYGQRFMWLWNKIHDPANGYFNQ FT DGIPYHSVETLICERPDYGHLTTSEAFSYYVWLEAVYGKLTGDWSKFKTAWDTLEKYMI FT PSAEDQPMRSYDPNKPATYAGEWETPDKYPSPLEFNVPVGKDPLHNELVSTYGSTLMYG FT MHWLMDVDNWYGYGKRGDGVSRASFINTFQRGPEESVWETVPHPSWEEFKWGGPNGFLD FT LFIKDQNYSKQWRYTDAPDADARAIQATYWAKVWAKEQGKFNEISSYVAKAARMGDYLR FT YAMFDKYFKPLGCQDKNAAGGTGYDSAHYLLSWYYAWGGALDGAWSWKIGSSHVHFGYQ FT NPMAAWALANDSDMKPKSPNGASDWAKSLKRQIEFYRWLQSAEGAIAGGATNSWNGRYE FT KYPAGTATFYGMAYEPNPVYHDPGSNTWFGFQAWSMQRVVEYYYVTGDKDAGALLEKWV FT SWVKSVVKLNSDGTFAIPSTLDWKRQPDTWNGAYTGNSNLHVKVVDYGTDLGITASLAN FT ALLYYSAGTKKYGVFDEGAKNLAKELLDRMWKLYRDEKGLSAPEKRADYKRFFEQEVYI FT PAGWIGKMPNGDVIKSGVKFIDIRSKYKQDPDWPKLEAAYKSGQAPEFRYHRFWAQCDI FT AIANATYEILFGNQ" FT RBS 5274..5280 FT CDS 5292..>5513 FT /pseudo FT /transl_table=11 FT /gene="manA" FT /product="beta-D-mannanase" FT /EC_number="3.2.1.78" FT /db_xref="PSEUDO:CAB06787.1" XX SQ Sequence 5513 BP; 1821 A; 949 C; 1470 G; 1273 T; 0 other; ggttcgttta actatggtga agctttacaa aaagctatca tgttttacga atttcaaatg 60 tctggtaaac ttccgaattg ggtacgcaac aactggcgtg gcgactcagc attaaaggat 120 ggtcaagaca atgggcttga tttgacaggt ggttggtttg acgcaggtga tcacgtcaag 180 tttaaccttc caatgtcata cactggtaca atgttgtcat gggcagtgta tgagtacaaa 240 gatgcatttg tcaagagtgg tcaattggaa catatcttaa atcaaatcga atgggttaat 300 gactattttg taaaatgcca tccaagcaaa tatgtatact attaccaggt tggggatgga 360 agtaaagatc atgcatggtg gggacctgct gaggttatgc aaatggagag accttcattt 420 aaggtcaccc aaagcagtcc tggatctaca gtagtaacag agacagcagc ttccttagca 480 gcagcttcaa ttgttttgaa agacagaaat cccactaaag cagcaacata tctgcaacat 540 gcaaaagaat tatatgagtt tgcagaagta acaaaaagcg atgcaggtta cactgctgca 600 aatggatatt acaattcatg gagcggtttc tatgatgagc tttcttgggc agcagtttgg 660 ttgtatttgg caacaaatga ttcaacatat ctcacaaaag ctgagtcata tgtccaaaat 720 tggccaaaaa tttctggcag taacacaatt gactacaagt gggctcattg ctgggatgat 780 gttcacaatg gagcggcatt attgttagca aaaattaccg gtaaggatat ttataagcaa 840 attattgaaa gtcacttaga ttactggatc acaggataca atggcgaaag gattaagtat 900 acaccaaaag gattagcatg gcttgatcaa tggggttcgt tgagatatgc aacaactaca 960 gcatttttgg catttgttta tagcgattgg gttggctgtc caagcacaaa aaaagaaata 1020 tatagaaaat ttggagaaag ccagattgat tatgcgttag gctcagctgg aagaagcttt 1080 gttgttggat ttggtacaaa tccaccaaag agaccgcatc acagaactgc tcatagctca 1140 tgggcagaca gtcagagtat accttcatat cacagacata cattatatgg agcgcttgtt 1200 ggtggtccag gctctgatga tagctacaca gatgatataa gcaactatgt gaacaatgag 1260 gttgcatgtg attataatgc agggtttgtg ggtgcattag caaagatgta tcaattgtac 1320 ggtgggaatc caataccaga tttcaaagct attgaaactc caacaaacga cgaattcttt 1380 gttgaagctg gtataaatgc atccggaact aactttattg aaattaaagc gatagttaat 1440 aaccaaagtg gttggcctgc caaagcaaca gataagctta aatttagata ttttgttgac 1500 ctgagtgaat taattaaagc aggatattca ccaaatcaat taaccttgag caccaattat 1560 aatcaaggtg caaaagtaag tggaccttat gtatgggatg caagcaaaaa tatatactac 1620 attttagtag actttactgg cacattgatt tatccaggtg gtcaagacaa atataagaaa 1680 gaagtccaat tcagaattgc agcaccacag aatgtacagt gggataattc taacgactat 1740 tctttccagg atataaaggg agtttcaagt ggttcagttg ttaaaacaaa atatattcca 1800 ctttatgatg gagatgtgaa ggtatggggt gacggaccag gaacttctgg agcaacaccg 1860 acaccaacag caacagcaac accaacacca acgccgacag taacaccaac accgactcca 1920 acaccaacat caactgctac accaacaccg acaccaacac cgacagtaac accaaccccg 1980 actccgacac cgactgctac accaacatca actccaacac caacatcgac gccgagcagc 2040 acacctgtag caggtggaca gataaaggta ttgtatgcta acaaggagac aaatagcaca 2100 actaatacga taaggccatg gttgaaggta gtgaacactg gaagcagcag catagatttg 2160 agcagggtaa cgataaggta ctggtacacg gtagatgggg acaaggcaca gagtgcgata 2220 tcagactggg cacagatagg agcaagcaat gtgacattca agtttgtgaa gctgagcagt 2280 agcgtaagtg gagcggacta ttatttagag ataggattta agagtggagc tgggcagttg 2340 caggctggca aagacacagg ggagatacag ataaggttta acaagagtga ttggagcaat 2400 tacaatcagg ggaatgactg gtcatggatg cagagcatga cgaattatgg agagaatgtg 2460 aaggtaacag cgtatataga tggtgtattg gtatggggac aggagccgag tggagcgaca 2520 ccaacaccga cagcgacacc agcaccgaca gtgacaccga cacctacacc aacaccaacg 2580 tcaacaccaa ctgctacacc aacagcaacg ccaacaccaa caccgacgcc gagcagcaca 2640 cctgtagcag gcgggcagat aaaggtattg tatgctaaca aggagacaaa tagcacaaca 2700 aacacgataa ggccatggtt gaaggtagtg aacactggaa gcagcagcat agatttgagc 2760 agggtaacga taaggtactg gtacacggta gatggggaca aagcacagag tgcgatatca 2820 gactgggcac agataggagc aagcaatgtg acattcaagt ttgtgaagct gagcagtagc 2880 gtaagtggag cggactatta tttagagata ggatttaaga gtggagctgg gcagttgcag 2940 gctggtaaag acacagggga gatacagata aggtttaaca agagtgactg gagcaattac 3000 aatcagggga atgactggtc atggatgcag agcatgacga attatggaga gaatgtgaag 3060 gtaacagcgt atatagatgg tgtattggta tggggacagg agccgagtgg agcgacacca 3120 acaccgacag caacaccagc accgacagtg acaccgacac ctacaccaac accaacgtca 3180 acaccaactg ctacaccaac agcaacgcca acaccaacac cgacgccgag cagcacaccg 3240 agtgtcgttg gcgaatatgg gcagaggttt atgtggttat ggaacaagat acatgatcct 3300 gcgaacgggt attttaacca ggatgggata ccatatcatt cggtagagac attgatatgc 3360 gaacgacctg attatggtca tttgaccacg agtgaggcat tttcgtacta tgtatggtta 3420 gaggcagtgt atggtaagtt aacgggtgac tggagcaaat ttaagacagc atgggacaca 3480 ttagagaagt atatgatacc atcagcggaa gatcagccga tgaggtcata tgatcctaac 3540 aagccagcga catacgcagg ggagtgggag acaccggaca agtatccatc gccgttggag 3600 tttaatgtac ctgttggcaa agacccgttg cataatgaac ttgtgagcac atatggtagc 3660 acattaatgt atggtatgca ctggttgatg gacgtagaca actggtatgg atatggcaag 3720 agaggggacg gagtaagtcg ggcatcattt atcaacacgt tccagagagg gcctgaggag 3780 tctgtatggg agacggtgcc gcatccgagc tgggaggaat tcaagtgggg cggaccgaat 3840 ggatttttag atttgtttat taaggatcag aactattcga agcagtggag atatacggat 3900 gcaccagatg ctgatgcgag agctattcag gctacttatt gggcgaaagt atgggcgaag 3960 gagcaaggta agtttaatga gataagcagc tatgtagcga aggcagcgag gatgggagac 4020 tatttaaggt atgcgatgtt tgacaagtat ttcaagccat taggatgtca ggataagaat 4080 gcggctggag gaacggggta tgacagtgca cattatctgc tatcatggta ttatgcatgg 4140 ggtggagcat tggatggagc atggtcatgg aagataggga gcagccatgt gcactttgga 4200 tatcagaatc cgatggcggc atgggcatta gcgaatgata gtgatatgaa gccgaagtcg 4260 ccgaatggag cgagtgactg ggcaaagagt ttgaagaggc agatagaatt ttacaggtgg 4320 ttacagtcag cggagggagc gatagcagga ggcgcgacaa attcatggaa tggcagatat 4380 gagaagtatc cagcagggac agcaacattt tatggaatgg catatgaacc gaatccggta 4440 tatcatgatc ctgggagcaa cacatggttt ggattccagg catggtcgat gcagagggta 4500 gtggagtatt actatgtgac aggagataag gacgcaggag cactgcttga gaagtgggta 4560 agctgggtta agagtgtagt gaagttgaat agtgatggta cgtttgcgat accgtcgacg 4620 cttgattgga agcgacaacc tgatacatgg aacggggcgt atacagggaa tagcaactta 4680 catgttaagg tagtggacta tggtactgac ttaggaataa cagcgtcatt ggcgaatgcg 4740 ttgttgtact atagtgcagg gacgaagaag tatggggtat ttgatgaggg agcgaagaat 4800 ttagcgaagg aattgctgga caggatgtgg aagttgtaca gggatgagaa gggattgtca 4860 gcgccagaga agagagcgga ctacaagagg ttctttgagc aagaggtata tataccggca 4920 ggatggatag ggaagatgcc gaatggagat gtaataaaga gtggagttaa gtttatagac 4980 ataaggagca agtataaaca agatcctgat tggccgaagt tagaggcggc atacaagtca 5040 gggcaggcac ctgagttcag atatcacagg ttctgggcac agtgcgacat agcaatagct 5100 aatgcaacat atgaaatact gtttggcaat caataattaa gtaggtaaat gaaaatttag 5160 cggggtagca caactataag tatggtgtgc tacctcgcta aaatcctgta tggaggtgtt 5220 tagaaaatag tacaaaaaat aaagaggtat aatggcaaaa atgcataaaa aagagggggt 5280 taggttttaa gatgagagta aaaacaaaaa tggggaagaa atggttgagt atactatgta 5340 cagttgtttt tttattgaac attttgttta tagcaaatgt aacgaattta cccaaagttg 5400 gtgcggctac atctaatgat ggagtagtga agatagatac tagcacatta ataggaacaa 5460 atcacgcaca ttgctggtac agagataaac ttgagacggc attgcgagga ata 5513 // |
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