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EBI DbfetchID Z86103; SV 1; linear; genomic DNA; STD; PRO; 4547 BP. XX AC Z86103; XX DT 03-MAR-1997 (Rel. 51, Created) DT 22-SEP-1997 (Rel. 52, Last updated, Version 6) XX DE T.neapolitana celA and celB genes and two open reading frames XX KW celA gene; celB gene; cellulase; endo-1,4-beta-glucanase. XX OS Thermotoga neapolitana OC Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga. XX RN [1] RP 1-4547 RA Zverlov V.; RT ; RL Unpublished. XX RN [2] RC (sites) RX DOI; 10.1006/bbrc.1993.1974 RX PUBMED; 8352795. RA Dakhova O., Kurepina N., Zverlov V., Svetlichnyi V., Velikodvorskaya G.; RT "Cloning and expression in Escherichia coli of Thermotoga neapolitana genes RT coding for enzymes of carbohydrate substrate degradation"; RL Biochem. Biophys. Res. Commun. 194(3):1359-1364(1993). XX RN [3] RP 1-4547 RA Zverlov V.; RT ; RL Submitted (27-FEB-1997) to the EMBL/GenBank/DDBJ databases. RL Zverlov V., Institute of Molecular Genetics, Russian Academy of Sciences, RL Kurchatov Sq., Moscow, Russia, 123182 XX FH Key Location/Qualifiers FH FT source 1..4547 FT /organism="Thermotoga neapolitana" FT /strain="Z2706-MC24" FT /mol_type="genomic DNA" FT /db_xref="taxon:2337" FT CDS <1..326 FT /pseudo FT /codon_start=3 FT /transl_table=11 FT /gene="orf1" FT /db_xref="PSEUDO:CAB06780.1" FT RBS 756..761 FT CDS 767..2332 FT /transl_table=11 FT /gene="orf2" FT /product="hypothetical protein" FT /db_xref="UniProtKB/TrEMBL:P96490" FT /protein_id="CAB06781.1" FT /translation="MKSIEGLKETFKYGAFSLPAVNYMLLEENLPKECREVLCILKLAW FT KGNFKEAIQKTDKILKSCKSEAAKYFLLANKLAFLKYTGKVDMNLYRYLKRNLPKMSKS FT IRDTVIVTLINFEASGIKPLRKVRVWKNDYRKSTLSFLYLSLARREADSGRLSEAVHDY FT IQAYRLSREVPHPTCIVSSLNDLAWDIREKHPKLAHALSQGAVFWLGYYREEPGNLFGA FT LDTLFVVEKDMDSPSIHSTAHIIVSLPVPEDYLSLLKKAKKFVLDYTGSTYPNTSQLRR FT YVEKVAWKGKTLSSKGISDILKGKTKMIRADTIRKLLTSGVDTGAPFPVWNEWIKMEIE FT RKYRESSEKLKELPLSSETNPFFLTTYMALLDREFLSRKERLKRAYKLLEDIESFADFM FT AKDHRTMEFVVSMVKAHPFIEGRKETVKRALGRMKRKRLERFTLRYIEMKESDRKLLDR FT FLRNYGRYDGVRFGVRLKGPDVVREFARRYSLKVQPLLVAFWCEEDGRVRRRLETILRY FT MVQG" FT RBS 3354..3358 FT CDS 3367..4140 FT /transl_table=11 FT /gene="celA" FT /standard_name="cellulase" FT /product="endo-1,4-beta-glucanase" FT /function="hydrolysis of beta-1,4-glucoside bonds" FT /EC_number="3.2.1.4" FT /db_xref="GOA:P96491" FT /db_xref="InterPro:IPR002594" FT /db_xref="UniProtKB/TrEMBL:P96491" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAB06783.1" FT /translation="MVELTAPGTADFRWNDMPLSMELNLWNVERYTGTVVMRFDGERLT FT FNGDVEDLSAREPERYILGYPEFYYGYKPWERHAAEGTKLPLVVSSVESFTVELSFEID FT HMPSLPLNFAMETWLTREKYQVEASIGDVEIMVWFYFNELTPGGKKVGEYTVSFELNGE FT QKRGIWELWHAEWNWDYLAFRLKNPVRKGRVRFNVKDFLDVAGEYLSRSTRVKDFDDLY FT FTVWEIGTEFGSPETKSARFGWTFNNFSIDMEVKG" FT RBS 4125..4129 FT CDS 4137..>4547 FT /transl_table=11 FT /gene="celB" FT /standard_name="cellulase" FT /product="endo-1,4-beta-glucanase" FT /function="hydrolysis of beta-1,4-glucoside bonds" FT /EC_number="3.2.1.4" FT /db_xref="GOA:Q798L1" FT /db_xref="InterPro:IPR002594" FT /db_xref="UniProtKB/TrEMBL:Q798L1" FT /protein_id="CAB06782.1" FT /translation="MRLVVSFLLVVSAFLFSAEVVLTDIGATDITFKGFPVTMELNFWN FT VKSYEGETWLKFDGEKVQFYADIYNIVLQNPDSWVHGYPEIYYGYKPWAAHNSGTEILP FT VKVKDLPDFYVTLDYSIWYENDLPINLAMETWI" XX SQ Sequence 4547 BP; 1350 A; 948 C; 1016 G; 1233 T; 0 other; ttataaagaa gagaagttct catgtttcat cccccaaaat gaaccatttc ttgccctttg 60 tacaaatttt catcctttac ctctcacttg agccatcggt ctttttcgct tctcctaaaa 120 agtatattaa aagatattat atacactctc tcaaaaagtt tgcatttcgt tccctgcgtc 180 tgtcttgcca tcagaatcaa aatcctcctc aacaactcag gaactttccc acatctgcac 240 ggtgccaata tttctcaccc cccgcacaga tgctaaaaca ccctatatgg ttttcaagtt 300 ttaaagaaaa ttcttcaatg atttgaaaag aacttacagg gctgtttttt agatcgcaaa 360 atttctgtga acttaaataa aaatgatttt ttacgtacag cagaacaagg gttaaaaaac 420 tctgatttcc agcagttgaa cctttgaaag taacctcaca agtagctgca ctttttgggg 480 tgcaacctga acctgttccg taatctcact tttccaggaa tgcgtttcgt tatctgtctt 540 cactttgtca gcgaaaatga ttgtagacag gagaacgagt atcaaaaaga ttatcatcaa 600 aacccttcgc attctgatca cctgtcatca ctttatcaaa ccttatatgg ttttcaagtt 660 ttggctgttc caaaatcctc tttaaaagca cattttcagc accagatttc atgggctttg 720 aaacaatttg gttttttgag gtataactat aacagaggga gtaaacatga aaagtataga 780 aggcctgaaa gagacattca agtacggtgc tttttctctt ccagcagtga attacatgct 840 actcgaagaa aaccttccaa aagagtgcag agaggttctc tgtatactga aactcgcttg 900 gaaaggaaac ttcaaagaag cgattcagaa aacagataaa attttgaaaa gctgcaaatc 960 cgaagcagcg aaatattttc ttttggcgaa caaactggca tttttaaaat atacaggcaa 1020 agtagatatg aatctgtaca ggtacctgaa aagaaacctt cccaagatgt caaaatcaat 1080 aagagatact gtgatcgtta ctttaataaa ctttgaagca agcggaataa agccactacg 1140 gaaggtgagg gtgtggaaaa acgattacag aaaatccaca ctttcctttt tatatctttc 1200 cctggcaaga agggaagcag attctggaag actctctgag gcggtacacg attacataca 1260 ggcctacaga ctatccaggg aggttcctca cccaacctgt attgtttcct ccctgaacga 1320 tcttgcatgg gatatcaggg aaaaacatcc aaaacttgcc catgctcttt ctcaaggagc 1380 cgttttctgg ctaggatact acagagagga accaggaaat ctctttggag ccctggatac 1440 tctgtttgtt gtggaaaaag acatggattc accgtctatc cacagcactg cacatatcat 1500 cgtttcgctt cccgtaccag aagattatct gtctctactc aaaaaagcga agaaatttgt 1560 gttggactac acaggatcaa cttatccgaa cacaagtcag ttgagaagat atgttgaaaa 1620 ggtcgcttgg aaaggaaaaa ctctcagctc caaaggtatc tcagatatat tgaaagggaa 1680 aacaaaaatg attcgtgcag atacgataag aaaactgttg acatctggtg ttgatacagg 1740 ggcacctttt cctgtctgga acgagtggat aaagatggag attgaaagaa aatacaggga 1800 gtcgtcagaa aagcttaaag agctccccct ttcatcagag acaaatccct tttttcttac 1860 cacctacatg gctcttcttg atagagagtt cctttcaagg aaagaaagac tcaaaagggc 1920 atataagctt cttgaagata ttgagtcgtt cgctgatttc atggcaaaag atcaccggac 1980 catggaattt gttgtttcaa tggtgaaggc tcatcctttc attgaaggaa ggaaagagac 2040 agtgaagaga gcccttggaa ggatgaaaag aaagagactg gaaagattca cgctaaggta 2100 catcgagatg aaagagtccg acagaaaact ccttgacaga tttctgagga actacggaag 2160 gtatgatggg gtgaggtttg gtgtcaggct aaaggggccg gatgtggtga gggagtttgc 2220 aaggaggtac tctctcaagg ttcaacctct gcttgttgcc ttctggtgcg aagaggacgg 2280 cagggtgaga agaagactgg aaacaatctt gaggtacatg gttcagggct gaacccagcg 2340 ttccagtctt ttttgaatct ttatttgatt gaaatcgctg tagcgtctgt cacgatggaa 2400 aaaatgagag atgaaaaact tgcgtaaaag atgtgatcac agtacaaaac taccggggga 2460 tgttccggca gacttacaac cccaagcatt caatccccct ttgcatattc aaaaaagaag 2520 attttttatt tattcaatta taaagcacga gagcgatgat cacgggaagg aaagcgtgtt 2580 tttctggtct gtttggtttt tcttagcaac actttttgct tgtaagctgt gctggtggaa 2640 tctttcaggg aacaactgat actctttctt cagatgaggc aaacaaactc atagaagagc 2700 ttttaatggc caacgaaaac acatcatcac ttgatattct agaccctgac agaaaaaaat 2760 cagtcccgca agcccttctc cgtccctggt caggaagaca aagcgaaaaa agaacttctg 2820 gcgaaggtat cctgtggctt cccaggtagt gcggtgttgc acccgccaga ggtgtgaggt 2880 tcaatccgca cctctgtggc catggaacac ctgctgctcc atggacaggc ctagagctgc 2940 caggaatccc atctccttca ggggattgga agtgtcaaaa aaccattttt gttcagaaaa 3000 cctactttct ttctctgaga tgcacaacac aggatggaac agaaacatct gtccactggc 3060 catgatactg gttggggatg actattattt cttctcgata gacatcgtgg atacaggatc 3120 agggacggaa gtcaggatat atccggaacc ctttttatca ctgtaatcgt tcaaaaccct 3180 gccttccgct tcgaacttga tctgtccaca gatgctggcc tcaaagcgtg ttcatcaact 3240 ttctgagaaa aacatgtttt tttgcggtat ttctggaata ttcaacgctc tttccgcata 3300 atctccgttt tttgcccttt gaatgtaaac attttcataa tagactgttt cttggaggtg 3360 ataaacgtgg tggaactgac cgcaccagga acggcagatt ttcgctggaa tgacatgccg 3420 ctttccatgg agctgaatct gtggaacgta gaaagataca cgggaacggt ggtgatgaga 3480 ttcgacggtg aaagactcac cttcaacggt gatgttgaag atctttctgc cagagagcca 3540 gaaaggtaca tccttggcta tcctgagttt tactacggat acaaaccctg ggaaagacat 3600 gcagcagagg ggacaaagtt acctctggta gtgtcttctg tggagtcctt cacggtcgaa 3660 ctttccttcg aaatagacca catgccctct cttccgctga actttgccat ggaaacctgg 3720 ctcacaagag aaaaatacca ggtcgaagct tccatcggag atgtggagat catggtgtgg 3780 ttctatttca acgaactcac accgggtgga aagaaagtgg gagagtacac cgtttcgttc 3840 gaacttaacg gagaacaaaa acgtggaatc tgggaactct ggcacgccga atggaactgg 3900 gattatcttg ctttccggct gaagaatcct gtgagaaagg ggagggtgag attcaacgtg 3960 aaggattttc tcgatgttgc aggagagtat ctttcaaggt ccacacgtgt gaaggatttt 4020 gatgatctgt atttcaccgt ctgggagatc ggaaccgaat ttggaagccc tgaaacaaag 4080 agtgcccgtt ttggatggac gttcaataac ttttcaatcg atatggaggt gaaaggatga 4140 ggttggtcgt ctcttttctt cttgtggttt ctgcctttct tttctcggca gaagtcgttc 4200 tcacagacat tggtgcaaca gacattacgt tcaaaggctt tccggtgacc atggaactca 4260 acttctggaa cgtcaaatcc tacgaaggag aaacgtggct caagttcgat ggtgaaaagg 4320 tccagttcta cgcggatatc tacaacatcg ttcttcaaaa cccggacagc tgggttcacg 4380 gctatccaga gatatactac ggctacaagc cgtgggcggc gcacaacagc ggaacagaga 4440 ttcttccggt gaaggtgaaa gatcttcctg acttttacgt aacccttgat tattcaatct 4500 ggtacgagaa cgaccttccg atcaaccttg caatggaaac gtggatc 4547 // |
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