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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   Z83311; SV 1; linear; genomic DNA; STD; PRO; 1034 BP.
XX
AC   Z83311;
XX
DT   11-DEC-1996 (Rel. 50, Created)
DT   18-APR-2005 (Rel. 83, Last updated, Version 4)
XX
DE   E.coli dfr gene and partial intI1 gene
XX
KW   DFR gene; dihydrofolate reductase; integrase; intI1.
XX
OS   Escherichia coli
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Escherichia.
XX
RN   [1]
RX   PUBMED; 9736538.
RA   Adrian P.V., du Plessis M., Klugman K.P., Amyes S.GB.;
RT   "New trimethoprim-resistant dihydrofolate reductase cassette, dfrXV,
RT   inserted in a class 1 integron";
RL   Antimicrob. Agents Chemother. 42(9):2221-2224(1998).
XX
RN   [2]
RC   Revised by [3]
RA   Mignon D.;
RT   ;
RL   Submitted (04-JUL-1996) to the EMBL/GenBank/DDBJ databases.
RL   Mignon D., South African Institute for Medical Research, Medical
RL   Microbiology, Cnr Hospital and DeKorte Street, Johannesburg, Gauteng, South
RL   Africa, 2000
XX
RN   [3]
RP   1-1034
RA   Mignon D.;
RT   ;
RL   Submitted (14-MAY-1998) to the EMBL/GenBank/DDBJ databases.
RL   Mignon D., South African Institute for Medical Research, Medical
RL   Microbiology, Cnr Hospital and DeKorte Street, Johannesburg, Gauteng, South
RL   Africa, 2000
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1034
FT                   /organism="Escherichia coli"
FT                   /isolate="UI14"
FT                   /mol_type="genomic DNA"
FT                   /clone="pUK2412"
FT                   /db_xref="taxon:562"
FT   -35_signal      85..90
FT   -10_signal      108..113
FT   CDS             complement(<1..200)
FT                   /transl_table=11
FT                   /gene="intI1"
FT                   /product="class I integrase"
FT                   /function="site specific recombination of gene cassettes"
FT                   /db_xref="UniProtKB/TrEMBL:Q9R6Q1"
FT                   /protein_id="CAB05888.1"
FT                   /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAFI
FT                   RFHGVRHPATLGSSEVEAFLS"
FT   misc_recomb     336..342
FT                   /standard_name="cassette boundary"
FT   CDS             357..830
FT                   /transl_table=11
FT                   /gene="dfr"
FT                   /standard_name="DHFRXV"
FT                   /product="dihydrofolate reductase"
FT                   /function="trimethoprim resistance"
FT                   /EC_number="1.5.1.3"
FT                   /db_xref="GOA:P78218"
FT                   /db_xref="InterPro:IPR001796"
FT                   /db_xref="InterPro:IPR012259"
FT                   /db_xref="InterPro:IPR017925"
FT                   /db_xref="UniProtKB/Swiss-Prot:P78218"
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT                   /protein_id="CAB05887.1"
FT                   /translation="MKLSLMAAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVGR
FT                   KTFESMGALPNRKYAVVTRSSFTSSDENVLVFPSIDEALNHLKTITDHVIVSGGGEIYK
FT                   SLIDKVDTLHISTIDIEPEGDVYFPEIPSSFRPVFSQDFVSNINYSYQIWQKG"
FT   misc_recomb     825..928
FT                   /standard_name="cassette boundary (59-base element)"
XX
SQ   Sequence 1034 BP; 289 A; 227 C; 260 G; 258 T; 0 other;
     caggacagaa atgcctcgac ttcgctgctg cccaaggttg ccgggtgacg cacaccgtgg        60
     aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa actgtaatgc       120
     aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac       180
     ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttgtacagtc tatgcctcgg       240
     gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac       300
     gatgttacgc agcagggcag tcgccctaaa acaaagttaa cccctaagga agtatcgtga       360
     aactatcact aatggcagca atttcgaaga atggagttat cggaaatggc ccagatattc       420
     catggagtgc caaaggggaa caattactct tcaaagcgat tacctataat cagtggcttt       480
     tggtaggccg aaagactttc gagtcaatgg gggctttacc caaccgaaaa tatgccgttg       540
     taactcgttc aagcttcact tccagtgatg agaatgtatt ggtatttcca tctatcgatg       600
     aagcgctaaa tcatctgaag acgataacgg atcatgtgat tgtgtctggt ggtggtgaaa       660
     tatacaaaag cctgatcgat aaagttgata ctttacatat ttcaacaatc gacattgagc       720
     cagaaggtga tgtctatttt ccagaaatcc ccagtagttt taggccagtt tttagccaag       780
     acttcgtgtc taacataaat tatagttacc aaatctggca aaagggttaa caagtggcag       840
     caactgaccg ccaaaagtgt cacttgtttt gccaaaaagc cggcaaaaca agcgccaatt       900
     ttgtcggcag ctgtgccagg cgttaaacat catgagggaa gcggtgatcg ccgaagtatc       960
     gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc      1020
     cgtacatttg tacg                                                        1034
//
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