 |
|
EBI DbfetchID Z71474; SV 1; linear; genomic DNA; STD; PRO; 6752 BP. XX AC Z71474; XX DT 02-APR-1997 (Rel. 51, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 4) XX DE B.megaterium xylR gene XX KW Xyl repressor; xylose isomerase; xylose permease; xylulokinase. XX OS Bacillus megaterium OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-3933 RX DOI; 10.1007/BF00245346 RX PUBMED; 1719948. RA Rygus T., Scheler A., Allmansberger R., Hillen W.; RT "Molecular cloning, structure, promoters and regulatory elements for RT transcription of the Bacillus megaterium encoded regulon for xylose RT utilization"; RL Arch. Microbiol. 155(6):535-542(1991). XX RN [3] RP 1-6752 RA Schmiedel D.; RT ; RL Submitted (28-JUN-1996) to the EMBL/GenBank/DDBJ databases. RL Dagmar Schmiedel, Inst. f. Mikrobiol., Biochem. & Genetik, Lehrstuhl fuer RL Mikrobiologie, Universitaet Erlangen, Staudtstrasse 5, Erlangen, 91058, RL Germany XX RN [4] RP 1-6752 RX DOI; 10.1046/j.1365-2958.1997.2881654.x RX PUBMED; 9076741. RA Schmiedel D., Kintrup M., Kuster E., Hillen W.; RT "Regulation of expression, genetic organization and substrate specificity RT of xylose uptake in Bacillus megaterium"; RL Mol. Microbiol. 23(5):1053-1062(1997). XX FH Key Location/Qualifiers FH FT source 1..6752 FT /organism="Bacillus megaterium" FT /strain="DSM319" FT /mol_type="genomic DNA" FT /clone="pWH1500, pWH905" FT /db_xref="taxon:1404" FT -10_signal 1762..1767 FT -35_signal 1785..1790 FT CDS complement(520..1686) FT /transl_table=11 FT /gene="xylR" FT /product="xyl repressor" FT /db_xref="InterPro:IPR000600" FT /db_xref="UniProtKB/TrEMBL:O05180" FT /protein_id="CAA96093.1" FT /translation="MVIIQIADQALVKKMNQKLILDEILKNSPVSRATLSEITGLNKST FT VSSQVNTLLEKDFIFEIGAGQSRGGRRPVMLVFNKNAGYSIGIDIGVDYLNGILTDLEG FT NIILEKTSDLSSSSASEVKEILFALIHGFVTHMPESPYGLVGIGICVPGLVDRHQQIIF FT MPNLNWNIKDLQFLIESEFNVPVFVENEANAGAYGEKVFGMTKNYENIVYISINIGIGT FT GLVINNELYKGVQGFSGEMGHMTIDFNGPKCSCGNRGCWELYASEKALLASLSKEEKNI FT SRKEIVERANKNDVEMLNALQNFGFYIGIGLTNILNTFDIEAVILRNHIIESHPIVLNT FT IKNEVSSRVHSHLDNKCELLPSSLGKNAPALGAVSIVIDSFLSVTPIS" FT -10_signal 1813..1818 FT -35_signal 1790..1795 FT CDS 1912..3249 FT /transl_table=11 FT /gene="xylA" FT /product="xylose isomerase" FT /db_xref="GOA:O08325" FT /db_xref="HSSP:1A0D" FT /db_xref="InterPro:IPR018115" FT /db_xref="UniProtKB/Swiss-Prot:O08325" FT /protein_id="CAA96094.1" FT /translation="MVQTSTNKINHFESANKVLYEGKDSKNPLAFKYYNPEEVVGGKTM FT KDQLRFSVAYWHQFTADGTDPFGAATMQRSWDRYDGMDLAKARVEAAFQLFETLNVPFF FT AFHDRDIAPEGSTLQETNKNLDVIVTMIKEYMQTSNVKLLWNTANMFTNPRFVHGAATS FT CNADVFRYAAAQVKKGLETAKELGAENYVFWGGREGYETLLNTNLQLELDNLARFMHMA FT VDYATEIGYTGQFLIEPKPKEPTTHQYDTDAATTISFLRQYGLDKYFKLNLEANHATLA FT GHTFEHELRVARVQGLLGSVDANQGDPLLGWDTDEFPTDLYSTTLAMYEILQNGGLGSG FT GLNFDAKVRRGSFEQDDLLYAHVAGMDAFARGLKVAHKLVEDRVFENVINERYSSFKEG FT IGLEIVEGKANFHTLEQYAFKNPNIANKSGRQERLKSILNQYILEV" FT CDS 3308..4789 FT /transl_table=11 FT /gene="xylB" FT /product="xylulokinase" FT /db_xref="GOA:O05181" FT /db_xref="InterPro:IPR018485" FT /db_xref="UniProtKB/TrEMBL:O05181" FT /protein_id="CAA96095.1" FT /translation="MKYVIGIDLGTSAVKTVLVDTKGKICAVVSKNYPLLHEKTGYSEQ FT NPEEWVQQTASALSELIANANIKPEDIEGISYSGQMHGLVMLDENYNLLRNAILWNDTR FT TTEQCHHLYKVIGEKRLLKITKNQALEGFTLPKLLWVKEYEPAIFEKTAVFMLPKDYLR FT FKMTGFIHSEYSDAAGTLLLNISKKKWDDELCQLAGININLCPPLVESSECIGNISEAF FT SQKNGISHFNETYLQVELIMLAEQLAPVFYHQGKHYVVLGHRVLFFLMRNEAIETLYGK FT VHYFNHGKENAFYTMGVTLSAGHSLGWFKDNFAKNVTFEELLADLKNVPIGSNGLLFTP FT YLVGERTPHADATIRGSFIGMDASHKKRDFTRAVIEVLRFLNESIESSRNEKLRLCYHG FT EQMIMLNSSRRFNAKIVKLSTEQGPALGAAMLAAYGCGWYKLIGRVCNVFITSSKTYYP FT NAEHVQVYKQLYELYKEVYLQTNDLSKKLGEFKRK" FT CDS 5092..6513 FT /transl_table=11 FT /gene="xylT" FT /product="xylose permease" FT /db_xref="GOA:O05182" FT /db_xref="InterPro:IPR005828" FT /db_xref="UniProtKB/TrEMBL:O05182" FT /protein_id="CAA96096.1" FT /translation="MKQNRNSLYIFSITLVAAIGGLLFGYDTAVISGAEESLKVYLIDS FT LGLGSLAHGVTVSSALIGCIIGGLVSGYFATKLGRKQSLILAAILFIVSALGASYPEFL FT FFTKGEPTLSLLLAFNFYRIIGGIGVGLASAICPIYIGEIAPADIRGRLVSFNQFMIIF FT GMLVVYFVNWGIANGETLEWINDVGWRYMFASGAIPALLFAALLFLVPETPRYLAIQNQ FT DQKALAILTKINGSSEAKAILEDIKQTISTNVSSEKLLAYGKLVIVVGILLSVFQQFVG FT INVALYYAPRIFESMGAAKGSSMLQTIIMGLVNVIFTVIAILTVDRLGRKPLLITGSIG FT MAIGMFGVASMAFSNIIGIGTLVFIIIYTASFMMSWGPICWVLISEIFPNKIRGRAVAI FT AVAAQWAANYFISSTYPVMMEYSGGLTYGFYGLMSVLSALFVWKFVPETKGRTLEQMEN FT MWRKKQGASKSKKAI" XX SQ Sequence 6752 BP; 2251 A; 1114 C; 1332 G; 2055 T; 0 other; agatctgacg cgtgtaactg cgaaggtaaa acgggtgatt gaaaataaac taacaaatga 60 agaggaacga aagagaagtc tagaatttgt tacgtttata tcggatgaat tactgcaaaa 120 tgatacggag cctcgcacat ttattattca gaactctctt ttatcgctag agaaaatcca 180 tactctcgca cagaccaaag aggtagagga atataaagaa gtcataacta ctctgacaag 240 acatgattca taatttgaaa agcaggtaaa ctaacccaaa aggcaagact ctccgtagtt 300 tagaaaagag caggttgcta ataacatata aacagccagt tgccgttatg ataggtgact 360 ggctgcatgg gatgaaaagg tgagggtgga gacagacata acactcttaa tagaagaggg 420 taattctttc tcttttatag aaaatcaatt aattgaaagt agctccttca ttcttaagat 480 caacgtgata taggtttgct aacctttgcg ttcacttaac taacttatag gggtaacact 540 taaaaaagaa tcaataacga tagaaaccgc tcctaaagca ggtgcatttt ttcctaacga 600 agaaggcaat agttcacatt tattgtctaa atgagaatgg actctagaag aaacttcgtt 660 tttaatcgta tttaaaacaa tgggatgaga ttcaattata tgatttctca agataacagc 720 ttctatatca aatgtattaa ggatattggt taatccaatt ccgatataaa agccaaagtt 780 ttgaagtgca tttaacattt ctacatcatt tttatttgcg cgttccacaa tctcttttcg 840 agaaatattc ttttcttctt tagagagcga agccagtaac gctttttcag aagcatataa 900 ttcccaacag cctcgatttc cacagctgca tttgggtcca ttaaaatcta tcgtcatatg 960 acccatttcc ccagaaaaac cctgaacacc tttatacaat tcgttgttaa taacaagtcc 1020 agttccaatt ccgatattaa tactgatgta aacgatgttt tcatagtttt ttgtcatacc 1080 aaatactttt tcaccgtatg ctcctgcatt agcttcattt tcaacaaaaa ccggaacatt 1140 aaactcactc tcaattaaaa actgcaaatc tttgatattc caatttaagt taggcatgaa 1200 aataatttgc tgatgacgat ctacaaggcc tggaacacaa attcctattc cgactagacc 1260 ataaggggac tcaggcatat gggttacaaa accatgaata agtgcaaata aaatctcttt 1320 tacttcacta gcggaagaac tagacaagtc agaagtcttc tcgagaataa tatttccttc 1380 taagtcggtt agaattccgt taagatagtc gactcctata tcaataccaa tcgagtagcc 1440 tgcattctta ttaaaaacaa gcattacagg tcttctgccg cctctagatt gccctgcccc 1500 aatttcaaaa ataaaatctt tttcaagcag tgtatttact tgagaggaga cagtagactt 1560 gtttaatcct gtaatctcag agagagttgc cctggagaca ggggagttct tcaaaatttc 1620 atctaatatt aatttttgat tcattttttt tactaaagct tgatctgcaa tttgaataat 1680 aaccactcct ttgtttatcc accgaactaa gttggtgttt tttgaagctt gaattagata 1740 tttaaaagta tcatatctaa tattataact aaattttcta aaaaaaacat tgaaataaac 1800 atttattttg tatatgatga gataaagtta gtttattgga taaacaaact aactcaatta 1860 agatagttga tggataaact tgttcactta aatcaaaggg ggaaatgaca aatggtccaa 1920 actagtacta ataaaattaa tcattttgaa agcgcaaaca aagttttata cgaaggtaaa 1980 gattctaaaa atcctttagc ttttaaatac tataaccctg aagaagtagt aggcggtaaa 2040 acgatgaaag atcagctgcg tttttctgtt gcttactggc accagtttac agcagatggt 2100 acggatcctt ttggcgcagc tactatgcaa agatcttggg atagatatga tggaatggat 2160 ttagcaaaag cgagagttga ggcagctttt caactttttg aaacattaaa tgtgccattc 2220 ttcgcatttc atgatcgaga cattgctccg gagggcagta cgttacaaga aacaaataaa 2280 aatcttgatg ttatcgtcac tatgatcaaa gaatacatgc aaacaagtaa cgtaaagtta 2340 ttatggaaca cagcgaatat gtttacaaat ccccggttcg ttcatggtgc agctacttct 2400 tgtaatgcag atgtatttcg ttatgcggcg gctcaagtaa aaaaggggct tgaaacagca 2460 aaagaactag gcgcagaaaa ttatgtattc tggggcggcc gtgagggata tgaaacactg 2520 cttaacacaa atttacagtt agagttagat aacttagcca gatttatgca tatggctgta 2580 gattacgcaa ccgaaattgg atatacgggg cagtttttaa ttgagcctaa acctaaagag 2640 cctacgacac atcagtatga taccgatgca gcaacaacta tttcattttt aagacaatac 2700 ggattagata aatacttcaa acttaatcta gaagctaacc acgcaacgtt agctgggcat 2760 acatttgaac acgaattacg agtagctaga gtacaaggct tattaggctc agtggatgca 2820 aaccaaggtg accctctttt aggctgggat acagatgaat tcccaacgga tttatattct 2880 acaacgctag caatgtatga aattctgcaa aatggagggc tgggcagcgg aggattaaac 2940 tttgatgcta aagtacgcag aggttctttt gaacaagacg atttactcta tgctcatgta 3000 gcaggaatgg atgcgtttgc tcgaggattg aaagtagcac ataaattagt agaagatcgt 3060 gttttcgaaa atgttattaa cgagcgctac agcagcttta aagaaggaat tgggcttgag 3120 attgtggaag gaaaagctaa cttccataca ctggagcagt acgcgtttaa aaatccgaat 3180 attgcgaata aatcaggaag acaagaacgt ttaaagtcca ttttaaatca atatatttta 3240 gaagtttaaa agacaaacga gagtagaaac aatttctact ctcgtttata aacgcggagg 3300 tatcaagatg aaatatgtaa ttggaattga tctaggaaca agcgccgtaa aaacggtttt 3360 agttgataca aaaggcaaga tttgcgctgt ggtttctaag aattatcctc tccttcatga 3420 aaaaacaggg tatagcgaac agaatccaga agaatgggtt cagcagacgg ctagtgcttt 3480 aagtgaatta attgctaacg ctaatataaa acccgaagat atagaaggaa ttagttattc 3540 tggacaaatg catggccttg tgatgctgga tgaaaactac aacctattac gtaacgccat 3600 tttgtggaat gacacaagaa caacagaaca atgtcatcat ctctataaag ttattggaga 3660 aaaacgtctt ttaaagataa caaaaaacca ggctttagaa ggatttactc ttcctaagtt 3720 attgtgggta aaagagtatg aaccggctat atttgaaaaa acagctgtat ttatgctgcc 3780 taaagattat ttacgcttta aaatgaccgg ctttattcat agtgaatatt cagatgctgc 3840 cggtacatta ttattaaaca tcagcaagaa aaaatgggat gatgagcttt gccaattagc 3900 aggaatcaat attaatcttt gtccgccact agttgaatct agtgaatgca taggcaatat 3960 tagtgaagcg ttctctcaaa aaaacgggat tagccacttc aacgaaactt atttgcaggt 4020 ggagctgata atgcttgcgg agcaattggc gccggtattt tatcatcagg ggaaacacta 4080 tgtagtattg ggacatcggg tgttgttctt tcttatgagg aacgaagcga tagagacttt 4140 gtatggaaaa gtccattatt ttaatcatgg aaaagaaaat gccttttata cgatgggtgt 4200 tacgctatct gcggggcaca gtttaggctg gtttaaggac aatttcgcaa aaaacgtcac 4260 atttgaagaa ttattggctg acctaaaaaa tgtcccaatc ggctcaaacg gccttttatt 4320 taccccttac ttagtcgggg aacgtacacc gcatgcagat gctacgattc gcggaagttt 4380 tataggaatg gacgcctctc ataaaaagag agactttacg cgagcggtta ttgaggtatt 4440 acgtttctta aatgagtcaa ttgaatcttc gagaaacgag aaattacgtc tatgttatca 4500 tggcgagcaa atgatcatgc tcaattcaag cagacgtttt aatgcgaaaa tcgtaaaatt 4560 gtctactgaa caaggcccag cgttaggagc cgccatgcta gcagcttatg gctgtggctg 4620 gtataaactc attggaagag tgtgcaacgt atttattacg tcttcgaaaa catattatcc 4680 taacgcagaa catgtacaag tctataaaca gctttatgaa ttatataaag aagtctacct 4740 tcaaacaaac gacttaagta aaaagctagg cgaatttaaa aggaaataat aggttgcaaa 4800 atatgcaacc ttactttaaa aagaaacttc atatgaaagt agcttgtatc aattgattat 4860 ggttgtatat cgatatacct gacacaaaag gaagtataga aaatgctgta gtaagatttg 4920 attcactaga aaaatacgta cattggaggg gaggataaga gcagttagtc gaatagacga 4980 cgaaaatttc ttttgtgttc caagaatttg aaaaacgcat tcaaataagt gcgaactcag 5040 ttagaaatag aacttctact gttactctta actaactgct tttaacaacg catgaaacaa 5100 aatcgaaact cattatatat tttttctatt accttagttg ccgctatcgg agggttactg 5160 tttggatatg acacagctgt aatatctgga gctgaagaat cattaaaggt gtatcttatt 5220 gacagcttag ggttaggatc tttagcacac ggtgttactg tatcaagtgc tttaatagga 5280 tgtattattg gaggattggt atctggttat tttgcgacga aactaggacg caaacaatca 5340 cttatactag cagctattct ttttatagtc tcagcgttag gagcttctta tcctgagttt 5400 ttatttttca caaaagggga accgacactc tctttgttac tagcttttaa cttctaccgc 5460 attataggtg ggattggggt tggcttagca tcagccattt gtcccatcta tataggagaa 5520 attgcaccgg cagatattcg cggcagattg gtatcgttca atcaatttat gattattttt 5580 ggaatgttag tcgtttactt cgtgaattgg ggtattgcaa atggggaaac cttagaatgg 5640 attaatgatg taggttggag atacatgttt gcttcaggag ctattccagc tttactcttt 5700 gcagcactct tgtttttagt tcctgaaacg cctcgctact tagctataca gaatcaagat 5760 caaaaagctt tagccatact aactaaaatt aatggatctt cagaagcgaa agcaatttta 5820 gaagatatta aacaaacaat ttctaccaat gtttcgtctg aaaaattatt ggcatatgga 5880 aaattagtta tcgttgtagg tatactgctt tctgtatttc aacagtttgt aggaatcaat 5940 gtagctctat attatgctcc gcgtattttt gaaagcatgg gagcagctaa aggttcttct 6000 atgctgcaaa ccattattat gggattagta aacgttattt ttacagtaat tgcaattttg 6060 acagttgatc ggttgggaag aaaaccttta ttaataacag ggtctatcgg aatggccatt 6120 ggaatgttcg gtgtagcaag catggctttt tcaaacatca ttggtatagg gacgcttgta 6180 tttatcatta tttatacagc ttcctttatg atgtcatggg gaccaatttg ttgggtgctc 6240 atttcagaga tttttcctaa taaaattaga ggtcgtgctg tagctattgc cgtagctgct 6300 cagtgggcgg ctaactattt tatttcctct acatatcccg tgatgatgga atatagcgga 6360 ggattaacat atggttttta cggtttaatg agtgtacttt ctgctttatt tgtctggaaa 6420 tttgttcctg aaacgaaagg tagaactctc gaacaaatgg aaaatatgtg gaggaaaaaa 6480 caaggggctt caaagagtaa gaaggccata taaccattga gcttttactt agaggaaagg 6540 tgagtataaa actttcaaaa acacctaaac tatttttagg tatttttaat atgtaatctt 6600 tatgaagaaa gtggatagct tttttaaaag atattgctat agtgataagt ctctttgaaa 6660 atagaaatat agttttcttt atgaggagtg tttcatcgta aatataaata acggcgcaat 6720 gaattcacaa aaaatctggg ggcatccgct at 6752 // |
|