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EBI DbfetchID Z69782; SV 1; linear; genomic DNA; STD; PRO; 5535 BP. XX AC Z69782; XX DT 27-FEB-1996 (Rel. 46, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 3) XX DE A.thermophilum xylR gene, xynA gene and alfA gene XX KW alfA gene; endo-1,4-beta-xylanase; fructose-bisphosphate aldolase; KW xylose repressor; xylR gene; xynA gene. XX OS Anaerocellum thermophilum OC Bacteria; Firmicutes; Clostridia; Clostridiales; Anaerocellum group; OC Anaerocellum. XX RN [1] RP 1-5535 RA Zverlov V.V., Bronnenmeier K., Velikodvorskaya G.A.; RT ; RL Unpublished. XX RN [2] RP 1-5535 RA Zverlov V.V.; RT ; RL Submitted (26-FEB-1996) to the EMBL/GenBank/DDBJ databases. RL Zverlov V. V., Institute of Molecular Genetics RAS, Kurchatov Sq. 46, RL Moscow, Russia, 123182 XX FH Key Location/Qualifiers FH FT source 1..5535 FT /organism="Anaerocellum thermophilum" FT /strain="Z-1320" FT /mol_type="genomic DNA" FT /db_xref="taxon:31899" FT RBS 16..21 FT CDS 30..1229 FT /transl_table=11 FT /gene="xylR" FT /product="xylose repressor" FT /function="transcriptional repressor of xylose-utilizing FT enzymes" FT /db_xref="GOA:Q44406" FT /db_xref="InterPro:IPR000600" FT /db_xref="UniProtKB/Swiss-Prot:Q44406" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA93626.1" FT /translation="MGNHTLLKQINKLLVLKTILDNKIISRTKISKLVDLNKATVSNLT FT DELIKEGYVVEKGYGKSKGGRRPVLLQVNKDVGSIIGIDLGVDYIHIILSNFVGEVIFE FT EYANMKIGEDKEKLLRLLFDLIEKSVKKAPQTPKGILGIGIGVPGIIEKESGTVLLAPN FT LKWQNVPLRSIVQQKFNLPVYIDNEANAGALGEKWFGEWGKVSDLIYLSVGIGLGAGII FT IDNKLFRGAAGFAGEVGHTTINFQDDVCSCGNIGCLENFASERALLSVIKKLVKQGVED FT RYISWENVDEITPSRIIQAAKEGSRVCRMAILEVAEKMGIGVANLVNIFNPEMVIIGNK FT ASFFGELFLEKLREVINQRSFIAQFYNLKIEVSKLKDRAVVLGCIAMVISDMLSFPEYA FT " FT RBS 1232..1237 FT CDS 1244..3310 FT /transl_table=11 FT /gene="xynA" FT /standard_name="1,4-beta-D-xylan xylanhydrolase" FT /product="endo-1,4-beta-xylanase" FT /function="hydrolysis of 1,4-beta-D-xylosidic linkages in FT xylans" FT /EC_number="3.2.1.8" FT /db_xref="GOA:Q59150" FT /db_xref="HSSP:1HIZ" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/TrEMBL:Q59150" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA93627.1" FT /translation="MSVCLYKRLKRFSVLILVILFLIVTLIGIETTKTSRVIGETKKSF FT VEYNFENRFIAPFKASGKSMSLRIDNTTAAEGTFSLLASGRKQIDDGILLDVTNLIDYA FT NEYKITLYVYHKSSKMQRFVVSSEIETKSGKKTKLLCEKIIIPKSWKKLDANLNLTEQK FT GIKKVWLKVYVPTSTTNFYVDLFTLKVADKSHLIKFENFEDKSIAGFISQDKKCKLSIS FT KEKAYQGTYSIKVQQTVKRQNTTVILPVKGTFEKGKSYSISFYMHQSILKSLNFAVGIR FT FLESGKNMREIVLGRVTIPKNKWTEVFASYTPSLDSKIKDFVIFIRPLSDISYYYIDNF FT TISDDGWYSAVPDLDLPSLREKYKNYFKIGVAVPYRALTNPVDVAFIKRHFNSITAENE FT MKPEALEPIEDNFNFSIADEYLNFCRKNNIAIRGHNLLHQQTPNWFFENPQTGKKLTNS FT EKDKKILLERLKKYIQTVVSRYKGRIYAWDVVNEAIDENEPDGFRRSDWFNVLGPEYIE FT KAFIYAHQADPNAQLFYNDYSTENPVKREYIYKLIKSLREKGIPIHGVGLQCHISVSWP FT SVEEVEKTIKLFSSIPGIKIHVTEIDISVAKEYGEDIDEETKRYLLIQQARKLKDLFDV FT FKKYKNVVTSVSFWGLKDDYSWLQGDFPLLFDKDYQPKFAFWSLIDPSVVPEE" FT terminator 3322..3378 FT repeat_region 3371..3420 FT /rpt_type=DIRECT FT /rpt_unit_range=3371..3395 FT CDS 3486..4550 FT /transl_table=11 FT /product="hypothetical protein" FT /note="ORF3" FT /db_xref="GOA:Q44407" FT /db_xref="InterPro:IPR001347" FT /db_xref="UniProtKB/Swiss-Prot:Q44407" FT /protein_id="CAA93628.1" FT /translation="MLDNLETIAQNDPSGMFESVYNLPEQIQKAYEIGKNISVNVKAED FT IDKVVITGLGGSAIGGNLLRVFVLDKCKIPVIVNRDYVLPAYVDSKTLVIASSYSGNTE FT ETLSAYQDAKAKGAKIIAITTGGKLKEFAEKDGFDVITIPSGLQPRAALGYSFIPLLML FT FVKLGLIEPVDDQIEETVKVLSDLRERYKPEVPEEKNLAKRLTLKLWNKLPIIYGISGT FT TEVIAERWKGQICENSKSPAYFNVFSELNHNEIVGTESPKHILGLFEIVMLHDTEDHKR FT NAIRMDITKDLIKGVVSGVNDIYSIGNSRLARMFSLIYLGDYVSLYLATLYQNDPTPVK FT KIDILKNKLAEIKD" FT CDS 4572..5312 FT /transl_table=11 FT /product="hypothetical protein" FT /note="ORF4" FT /db_xref="GOA:Q44408" FT /db_xref="HSSP:1M65" FT /db_xref="InterPro:IPR003141" FT /db_xref="UniProtKB/Swiss-Prot:Q44408" FT /protein_id="CAA93629.1" FT /translation="MRAERKMFLEVETHCHTIASGHAYNNLEEMVLEAQKKGLKGICIT FT DHGPEMPGSCSSLYFYNLIVVPRKINGIMVFRGCEANIVDYEGRIDIPEDALKRLDFVI FT ASLHDVCIPSGTVSDHTRALIGAIKNPYIHCIGHPGNPLYEIDKEEVVLAAKEYKKAIE FT INNSSFYVREKSKENCIEILKLCKKYGVYIAMGSDAHYKADIGRCEITQKLVCEYEFPP FT ELIVNKGLESFISFLKLHGKDIDI" FT RBS 5326..5331 FT CDS 5341..>5535 FT /transl_table=11 FT /gene="alfA" FT /product="fructose-bisphosphate aldolase" FT /EC_number="4.1.2.13" FT /note="belongs to class II fructose-bisphosphate aldolases" FT /db_xref="GOA:Q59151" FT /db_xref="InterPro:IPR013785" FT /db_xref="UniProtKB/TrEMBL:Q59151" FT /protein_id="CAA93630.1" FT /translation="MPLVTTREMFKKAAEGKYAIGAFNVNNMEIIQGIVEAAKEEQAPL FT ILQVSAGARKYAKHIYLIKL" XX SQ Sequence 5535 BP; 1968 A; 812 C; 1181 G; 1574 T; 0 other; aagcttaatt agcaaaggaa aatgacagga tgggtaacca cacgctacta aagcaaataa 60 acaagctttt ggttttgaaa acaattttgg acaacaagat aatatctcgt acaaagatat 120 cgaaacttgt agatttaaat aaagcaacag tctcaaacct caccgatgag ctcataaaag 180 aagggtatgt agtagaaaaa ggatacggta agtccaaagg cggaagaaga cctgtccttt 240 tacaggtaaa caaagatgta ggttcaatca tcggaattga cttaggtgtt gactatattc 300 atattattct ctcaaacttt gtgggagaag ttatttttga agaatatgcc aacatgaaaa 360 taggtgagga taaggaaaaa cttttaaggc ttctttttga cctgattgaa aaatcagtaa 420 aaaaggcgcc acaaactcca aaagggattt taggtattgg aattggtgtt ccaggtatta 480 tagaaaaaga gtctggaact gtccttcttg ctcccaattt gaaatggcaa aatgtccctt 540 tgaggtcaat tgttcagcaa aagttcaacc tccctgttta tattgacaat gaagcaaatg 600 caggcgcact gggcgaaaag tggtttggtg agtggggaaa agttagtgat ttgatttatt 660 tgagtgttgg aattgggctt ggtgcaggaa ttattatcga caacaaactt ttcagaggtg 720 ctgcaggatt tgcaggtgaa gttggacata ccactatcaa ctttcaggac gatgtttgca 780 gctgcggcaa tattggctgt cttgagaact ttgcatccga gagggcactt ttgagtgtta 840 taaaaaagct tgtaaaacaa ggagtagagg ataggtatat aagctgggaa aatgtagatg 900 aaatcactcc ttctcggata atacaagcag caaaagaagg aagcagagtt tgcagaatgg 960 ctatacttga ggttgctgaa aagatgggga taggcgtggc aaatcttgta aatattttta 1020 atcctgaaat ggtaattata ggtaataagg catcattttt tggagagttg tttttagaaa 1080 aattgaggga agtaattaac cagagatcct ttatcgccca gttttataat cttaagatag 1140 aggtttcaaa actgaaagac agagctgtgg tgctgggatg tatagctatg gtgatttccg 1200 acatgctttc ttttccagaa tatgcatgag gaggaggtac taagtgagtg tttgtcttta 1260 taaaaggttg aaaaggtttt cagtattgat tttagtaatt ttatttttga ttgtaacctt 1320 aattggtata gaaactacta aaactagtag agtcattgga gaaacaaaaa agagttttgt 1380 ggagtataat tttgaaaata ggtttatagc accatttaaa gcgtcaggaa aatctatgag 1440 tttaaggatt gacaatacaa cagcagcaga agggaccttt tcacttcttg caagtggccg 1500 aaagcaaata gatgatggaa ttttgcttga tgtaaccaat cttatagatt atgcaaatga 1560 atataaaatt actctatatg tttatcacaa gtcaagcaaa atgcagcgtt ttgttgtgag 1620 tagcgaaatt gagacaaaaa gcggaaagaa aacaaaactg ctgtgtgaaa aaattattat 1680 tccaaaaagt tggaaaaaac ttgatgcaaa tttaaatttg actgagcaaa aagggataaa 1740 aaaagtttgg ctaaaggtat atgtgccaac ctcaaccaca aatttttacg ttgacctttt 1800 tacactcaaa gttgctgaca aatctcatct aataaaattt gaaaactttg aagataaaag 1860 tatagcagga tttatctctc aagataaaaa atgtaaatta tccatttcaa aagaaaaagc 1920 ttaccaaggt acatatagca taaaagttca gcaaacagtt aaaagacaaa acacaacagt 1980 tatcttgcct gtaaaaggaa catttgaaaa ggggaaaagt tattcaatat ctttttatat 2040 gcatcaatcc attctaaaga gtttaaattt tgcagttggt ataaggtttc tggaaagtgg 2100 aaagaatatg agagagattg tgcttgggag ggtaaccatt ccaaagaata agtggacaga 2160 agtatttgca agttatacgc cctctttgga ttcaaagata aaagactttg tgattttcat 2220 aagacccctc tcagatattt cctattacta tatcgacaac tttacaattt cagatgatgg 2280 atggtattca gctgtacctg atttagattt accttctttg agagagaaat acaaaaacta 2340 ctttaaaatt ggtgttgctg taccatacag ggctttgaca aatccagttg atgttgcttt 2400 cataaagaga cattttaaca gcattacagc agagaatgag atgaagccag aagcacttga 2460 accaatagag gacaatttca acttttcaat tgctgatgag tatcttaatt tctgcagaaa 2520 gaataatata gctattcgtg gtcacaactt gttgcatcag caaaccccaa actggttttt 2580 tgaaaaccct cagacaggga aaaagctgac gaatagtgaa aaagacaaaa agatactttt 2640 agagaggcta aaaaagtata tccagacagt tgtttcaaga tacaagggga gaatatatgc 2700 atgggatgtg gtaaatgaag ctattgatga gaatgagcca gatggattta gaagaagtga 2760 ctggtttaat gtattgggtc cagagtatat cgaaaaagcg ttcatatacg ctcatcaggc 2820 agaccctaac gctcagcttt tttacaatga ctacagcaca gaaaatccag taaagagaga 2880 atatatttac aagctaataa agagtctaag agaaaaagga atacccattc atggtgttgg 2940 tcttcagtgt catatttctg taagctggcc aagcgttgaa gaggtagaaa aaactataaa 3000 actttttagc agcattcctg gtattaagat acatgtcaca gaaattgata taagtgtggc 3060 aaaagaatat ggtgaagaca tagatgaaga aacaaaaaga tatcttttga ttcagcaggc 3120 aagaaagtta aaagatcttt ttgatgtgtt caaaaaatac aaaaatgtag taacaagtgt 3180 ttcgttctgg ggactcaaag atgactattc atggcttcaa ggtgattttc cacttttgtt 3240 tgacaaagat tatcaaccaa aatttgcttt ctggagctta atagacccgt cagttgtgcc 3300 agaagaataa aaagcagata gaataggaaa gcaccacaag aaggttttaa acttctttag 3360 aagtggtgct ttcctatttt ttgtgtggtt ataaattcct attttttgtg tggttataaa 3420 gttgaaaaaa ctgaaaaatt gttatattat gaaatagaaa attttaaaga aaggagtctg 3480 ggaaaatgct tgataattta gagacaattg cacagaatga cccaagcggt atgtttgagt 3540 cagtttataa tcttcctgag caaattcaaa aagcatacga aataggcaaa aatattagcg 3600 tgaatgtaaa agcagaagat atagataagg ttgtgattac aggtcttggc ggttcagcaa 3660 taggtggaaa ccttttgaga gtatttgtcc ttgataagtg caaaattcct gtgattgtta 3720 acagagacta tgtacttcct gcgtatgttg actctaaaac tcttgttata gcgtccagct 3780 actcaggtaa caccgaagag acactttctg cataccagga tgcaaaggca aaaggagcaa 3840 aaatcattgc aatcacaaca ggcggaaagt taaaagagtt tgccgaaaaa gacggatttg 3900 atgtaatcac aattccaagc gggcttcagc caagagctgc acttggatac tcattcattc 3960 cactcttgat gctatttgtc aaacttggtt tgattgaacc tgttgatgac cagatagaag 4020 agacagtaaa ggttttaagt gacttgagag aaagatataa accagaggtg ccggaagaga 4080 aaaaccttgc aaagagactt acactaaaac tttggaataa gctcccaatc atatatggca 4140 ttagtggaac cacagaggtc attgcagaga gatggaaggg tcagatttgt gaaaattcaa 4200 aatcaccagc atatttcaat gtgttttcag aactcaacca caacgagata gttggaactg 4260 agtcgccaaa acatattctt ggactttttg aaattgtaat gctccatgac acagaagacc 4320 acaagagaaa tgcaatcaga atggatatta caaaagacct tataaagggt gttgtgtctg 4380 gtgtaaatga tatatactca atcggaaatt caagacttgc aagaatgttt tctctaatct 4440 acttaggcga ctatgtatcg ctttatcttg caacacttta tcagaatgac ccaactcctg 4500 tgaaaaagat tgatattttg aagaacaaac ttgcagagat taaagattaa tgtatttgac 4560 aaaaattttt gatgagagct gagagaaaga tgtttttgga agttgagacg cactgtcata 4620 ccattgcaag cggtcatgct tacaataact tggaagagat ggtacttgag gcacagaaaa 4680 aaggtttgaa agggatatgt attacagacc acggccctga gatgccaggg tcgtgtagca 4740 gtttatattt ttacaatcta attgtggtac ctagaaaaat aaatggtata atggtattta 4800 gagggtgcga ggcaaatatt gttgactacg aaggcagaat agatatcccg gaagatgccc 4860 tgaaaaggct tgattttgtg attgcaagcc tgcatgatgt ttgtatcccg agtgggacag 4920 tttctgacca taccagggca ctcattggtg ctatcaaaaa tccgtatata cactgtatag 4980 ggcatccggg gaatccgcta tatgaaattg acaaagaaga ggttgtgctt gctgcaaagg 5040 aatataaaaa agcaattgag ataaacaact cttcgtttta tgttcgtgaa aaaagtaaag 5100 aaaattgcat agaaatttta aagctgtgca aaaaatatgg tgtgtacatt gctatgggct 5160 cagatgcaca ctacaaagca gacattggca gatgtgaaat tactcaaaaa cttgtgtgtg 5220 aatatgaatt tccgcctgag cttattgtca acaaaggctt agaaagcttt ataagctttc 5280 taaagcttca tggaaaggat attgatattt aaaacaaaaa ctttgaggag gtaatttaaa 5340 atgccgttag ttaccacaag agaaatgttt aaaaaagcgg ccgaaggcaa gtacgcaata 5400 ggtgcattta acgtcaacaa catggagatt atccagggaa ttgttgaggc tgcaaaggag 5460 gaacaggcac cgcttatttt gcaggtttca gcaggtgcaa gaaagtatgc aaagcacatc 5520 tatcttatca agctt 5535 // |
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