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EBI DbfetchID Z69359; SV 1; linear; genomic DNA; STD; PRO; 3120 BP. XX AC Z69359; XX DT 05-FEB-1996 (Rel. 46, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 5) XX DE C.stercorarium celY and celZ genes XX KW avicelase I; avicelase II; celY gene; celZ gene; endo-beta-1,4-glucanase; KW exo-beta-1,4-glucanase. XX OS Clostridium stercorarium OC Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; OC Clostridium. XX RN [1] RP 1-3120 RX PUBMED; 9084173. RA Bronnenmeier K., Kundt K., Riedel K., Schwarz W.H., Staudenbauer W.L.; RT "Structure of the Clostridium stercorarium gene celY encoding the RT exo-beta-1,4-glucanase Avicelase II"; RL Microbiology (Reading, Engl.) 143:891-898(1997). XX RN [2] RC (sites) RX DOI; 10.1111/j.1432-1033.1991.tb16195.x RX PUBMED; 1909625. RA Bronnenmeier K., Ruecknagel K.P., Staudenbauer W.L.; RT "Purification and properties of a novel type of exo-1,4-beta-glucanase RT (avicelase II) from the cellulolytic thermophile Clostridium stercorarium"; RL Eur. J. Biochem. 200(2):379-385(1991). XX RN [3] RP 2817-3120 RX DOI; 10.1007/BF00265062 RX PUBMED; 2250652. RA Jauris S., Ruecknagel K.P., Schwarz W.H., Kratzsch P., Bronnenmeier K., RA Staudenbauer W.L.; RT "Sequence analysis of the Clostridium stercorarium celZ gene encoding a RT thermoactive cellulase (Avicelase I): identification of catalytic and RT cellulose-binding domains"; RL Mol. Gen. Genet. 223(2):258-267(1990). XX RN [4] RP 1-3120 RA Bronnenmeier K.; RT ; RL Submitted (02-FEB-1996) to the EMBL/GenBank/DDBJ databases. RL Bronnenmeier K., Technische Universitaet Muenchen, Lehrstuhl fuer RL Mikrobiologie, Arcisstr.21, Muenchen, Germany, 80290 XX FH Key Location/Qualifiers FH FT source 1..3120 FT /organism="Clostridium stercorarium" FT /strain="NCIB 11754" FT /mol_type="genomic DNA" FT /db_xref="taxon:1510" FT RBS 60..65 FT /citation=[1] FT CDS 72..2816 FT /transl_table=11 FT /gene="celY" FT /standard_name="exo-beta-1,4-glucanase" FT /product="avicelase II" FT /EC_number="3.2.1.91" FT /db_xref="GOA:P50900" FT /db_xref="InterPro:IPR000556" FT /db_xref="InterPro:IPR001956" FT /db_xref="InterPro:IPR005102" FT /db_xref="InterPro:IPR008928" FT /db_xref="InterPro:IPR008965" FT /db_xref="InterPro:IPR012341" FT /db_xref="InterPro:IPR013783" FT /db_xref="InterPro:IPR014756" FT /db_xref="UniProtKB/Swiss-Prot:P50900" FT /citation=[1] FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA93280.1" FT /translation="MKRRLMKGISLLTLVFLIGIMLQLSLKSELTAYASSDDPYKQRFL FT ELWEELHDPSNGYFSSHGIPYHAVETLIVEAPDYGHLTTSEAMSYYLWLEALYGKFTGD FT FSYFMKAWETIEKYMIPTEQDQPNRSMAGYNPAKPATYAPEWEEPSMYPSQLDFSAPVG FT IDPIYNELVSTYGTNTIYGMHWLLDVDNWYGFGRRADRISSPAYINTFQRGSQESVWET FT IPQPCWDDLTIGGRNGFLDLFVGDSQYSAQFKYTNAPDADARAIQATYWANQWAKEHGV FT NLSQYVKKASRMGDYLRYAMFDKYFRKIGDSKQAGTGYDAAHYLLSWYYAWGGGITADW FT AWIIGCSHVHAGYQNPMTAWILANDPEFKPESPNGANDWAKSLERQLEFYQWLQSAEGA FT IAGGATNSYKGRYETLPAGISTFYGMAYEEHPVYLDPGSNTWFGFQAWTMQRVAEYYYL FT TGDTRAEQLLDKWVDWIKSVVRLNSDGTFEIPGNLEWSGQPDTWTGTYTGNPNLHVSVV FT SYRTDLGAAGSLANALLYYAKTSGDDEARNLAKELLDRMWNLYRDDKGLSAPETREDYV FT RFFEQEVYVPQGWSGTMPNGDRIEPGVTFLDIRSKYLNDPDYPKLQQAYNEGKAPVFNY FT HRFWAQCDIAIANGLYSILFGSEQANDSFITPTSATFDKNNQEDISVTVTYNGNTLLGI FT KSGSSYLIEGVDYIVNGDVIIIKKEFLAGQATGSISLLFDFSAGLDRTLTIDIIDTGGG FT EEPVEPVEPVEGVLIIQSFNANTQEISNSIMPRFRIYNSGNTSIPLSEVKLRYYYTVDG FT DKPQNFWCDWASIGSSNVTGTFVKMDGATTGADYYLEIGFTPQAGTLEPGASIEVQGRF FT SKIDWTDYTQTNDYSFNPTASSYVDFNKITAYISGNLVYGIEP" FT sig_peptide 72..171 FT /citation=[1] FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" FT mat_peptide 172..2813 FT /gene="celY" FT /product="avicelase II" FT terminator 2867..2903 FT /citation=[1] FT RBS 2941..2950 FT CDS 2958..>3120 FT /transl_table=11 FT /gene="celZ" FT /standard_name="endo-beta-1,4-glucanase" FT /product="avicelase I" FT /EC_number="3.2.1.4" FT /db_xref="GOA:Q59318" FT /db_xref="HSSP:1G87" FT /db_xref="InterPro:IPR001701" FT /db_xref="InterPro:IPR008928" FT /db_xref="InterPro:IPR012341" FT /db_xref="UniProtKB/TrEMBL:Q59318" FT /citation=[1] FT /citation=[3] FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA93281.1" FT /translation="MRKFWSFAIIISLLVTGLFIHTPKAEAAGYNYGEALQKAIMFYEF FT QRSGKLPEN" XX SQ Sequence 3120 BP; 902 A; 639 C; 770 G; 809 T; 0 other; gataactgcg gcaaatctgt agtcaaataa taagccggat accttcaatt aaataagaaa 60 ggggatcata aatgaagaga agattgatga aaggaatctc tttattgacc ctggtgtttt 120 taattggtat catgctccaa ttgtcactga aaagcgaact tacggcatat gcttcttcgg 180 atgatcccta taagcaacgc ttcttggaat tgtgggaaga acttcatgac ccttccaacg 240 gatatttcag ctctcatggg ataccatatc acgccgtcga gacattgata gtcgaggcac 300 ctgattatgg gcatcttacc accagtgaag ccatgagcta ttacctctgg ctggaagctt 360 tgtacggaaa atttaccgga gatttttcat attttatgaa ggcttgggag actatcgaaa 420 agtatatgat acctaccgaa caggatcagc ctaacagaag catggcagga tacaatcccg 480 ctaaacccgc cacatatgct cccgaatggg aagagcccag tatgtatcct tcacagctgg 540 atttttcagc tcccgtagga atagatccaa tttacaatga gctggtcagt acctatggaa 600 ccaatacgat ctacggaatg cactggctgc ttgacgtaga caactggtat ggtttcggaa 660 gacgggctga caggatcagt tcaccggcct atatcaatac gttccagaga ggaagccagg 720 aatctgtgtg ggaaacgatt ccgcagcctt gttgggatga tttgaccatt ggcgggagga 780 acggatttct ggatcttttc gtcggggaca gccaatattc ggcgcaattt aagtatacca 840 atgcgcctga tgcggatgcc agggcaattc aagcaactta ttgggctaat cagtgggcga 900 aggagcatgg tgtaaatctc agtcagtatg ttaaaaaagc ttccaggatg ggagactacc 960 tccggtatgc gatgtttgac aagtatttca gaaagatagg cgactcaaaa caggccggaa 1020 ccggttatga tgcagctcat tatctcttgt cctggtatta tgcgtggggt ggcggtatta 1080 cggcggactg ggcatggatt ataggctgca gccatgttca cgcgggttat cagaatccga 1140 tgaccgcatg gattctggca aacgatcctg aatttaagcc tgaatcgccc aacggcgcaa 1200 atgactgggc aaagagtttg gaacgtcagc tggaattcta tcagtggtta cagtcggctg 1260 aaggtgccat agccggcgga gcgaccaatt catataaagg acgttatgag actttgcctg 1320 caggtatatc cacattctat ggcatggctt atgaggaaca cccggtctac cttgatcccg 1380 gaagcaatac ctggtttgga ttccaggcat ggacaatgca aagagtggcc gaatattatt 1440 atctgacggg cgacaccaga gctgaacaac tgcttgacaa gtgggtggac tggattaaat 1500 ccgttgtaag gcttaacagc gacggaacat ttgaaatacc cggcaattta gaatggtccg 1560 ggcaacctga cacatggaca ggcacatata caggcaatcc taatcttcat gtaagtgtag 1620 tctcttacag aaccgatctg ggcgctgccg gttctcttgc caatgccttg ctatactacg 1680 caaaaacctc cggtgatgat gaagcccgga accttgcgaa agaactgctt gaccgtatgt 1740 ggaatctgta cagggatgac aaggggcttt cggctccaga aacaagggag gattatgtaa 1800 gattctttga acaggaagta tatgtaccgc aaggctggtc cggaaccatg ccgaatggcg 1860 acagaattga gccgggcgtc actttcctgg atattcggtc caaatatttg aacgatcctg 1920 attatccaaa gttgcagcag gcatacaatg agggcaaggc tcctgtattc aattatcaca 1980 gattctgggc acagtgtgat atcgcaatag caaacggttt gtattccatt cttttcggct 2040 cggagcaggc taatgattcg tttattactc ctacttcggc tacatttgat aaaaataatc 2100 aggaagacat tagtgtaacg gtaacatata acggcaatac cctgcttgga ataaaatccg 2160 ggtcttccta tcttatagaa ggtgtggatt atattgtaaa cggtgacgta ataatcataa 2220 agaaagaatt cctggccgga caagccacag gaagcatatc ccttcttttt gacttcagcg 2280 caggtcttga ccgtacgtta acgatagata tcattgatac cggcggcgga gaagagcctg 2340 tggagcccgt ggaaccggtt gaaggagtac ttataattca gtccttcaat gccaatactc 2400 aggagataag caattccata atgccaagat tcaggattta caatagcggg aatacaagta 2460 tacctttgtc tgaggtaaag ttaaggtatt actataccgt ggacggtgat aaaccccaga 2520 atttctggtg cgactgggca agcataggca gcagcaatgt aaccggaacc ttcgtgaaga 2580 tggacggtgc cacgaccggc gctgactatt accttgaaat cggttttaca ccacaggccg 2640 gaacccttga gcccggagct tcaattgagg ttcagggaag attttcaaag attgattgga 2700 ccgattatac tcagaccaat gattattcct ttaatccgac ggcaagcagt tatgtggatt 2760 ttaataaaat tacggcatac atttctggga atctggtata cggtatagag ccgtaatagc 2820 ggattaaaaa caacgggcat gatgggatat gacgttcaat tgatcataat ttcgggtgca 2880 tggttaccat gcacccggaa ttagacgttg tatatggtat aatacatttg aaaccgttga 2940 agggaggagg ttttaatatg cgaaaatttt ggtcctttgc aattataatc agtttacttg 3000 tcaccggtct ctttatacat actcctaagg ctgaagcggc aggatataat tatggggaag 3060 cattacaaaa agctattatg ttttatgaat ttcaaaggtc tggaaaactg ccggaaaaca 3120 // |
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