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EBI DbfetchID Z56283; SV 1; linear; genomic DNA; STD; PRO; 3240 BP. XX AC Z56283; XX DT 30-APR-1996 (Rel. 47, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 5) XX DE L.helveticus pepI gene XX KW pepI gene; proline iminopeptidase. XX OS Lactobacillus helveticus OC Bacteria; Firmicutes; Lactobacillales; Lactobacillaceae; Lactobacillus. XX RN [1] RP 1-3240 RX PUBMED; 9004508. RA Varmanen P., Rantanen T., Palva A.; RT "An operon from Lactobacillus helveticus composed of a proline RT iminopeptidase gene (pepI) and two genes coding for putative members of the RT ABC transporter family of proteins."; RL Microbiology (Reading, Engl.) 142:3459-3468(1996). XX RN [2] RP 1-3240 RA Varmanen P.; RT ; RL Submitted (17-OCT-1995) to the EMBL/GenBank/DDBJ databases. RL Varmanen P., Agrical Research Centre of Finland, 31600 Jokioinen, RL Jokioinen, Finland. XX FH Key Location/Qualifiers FH FT source 1..3240 FT /organism="Lactobacillus helveticus" FT /strain="53/7" FT /mol_type="genomic DNA" FT /db_xref="taxon:1587" FT -35_signal 132..137 FT -10_signal 155..160 FT RBS 176..180 FT CDS 184..1518 FT /transl_table=11 FT /gene="orf1" FT /db_xref="GOA:Q48560" FT /db_xref="InterPro:IPR003439" FT /db_xref="InterPro:IPR003593" FT /db_xref="InterPro:IPR017871" FT /db_xref="UniProtKB/TrEMBL:Q48560" FT /protein_id="CAA91229.1" FT /translation="MNVTFSYEKEPLINQLNMNIAPGFNLLIGPTGCGKSTLLKIIAGL FT YPKYAGKLTGKIDLHGQKAAMMFQNAAEQFTMTTPREEIIFALENLQIKAKDYDLHIKK FT AVEFTKIADLLDQKINTLSGGQQQHVALAVLIAMDVDVFLLDEPFASCDPNTRHFLIEK FT LASLAETGRTIILSDHVLDDYEKICDHLYQFEGKTVKELSANEKNKLFKQNKQFHEQSY FT SFALPTGTPVFELNKTQIKQNRLLLKQNKLKIYGKTTLITGSNGVGKTSLFKAMTKMIP FT YKGNFTYLDNEISKIKYRKYLSQIAQFFQKASDQFLTVTVKDEIELSKKDRNNFFTDAK FT IDEWLEKLQLKQHLDQVVYSLSGGQQKKLQILLMLMTKHNVLLIDEPLSGLDHESVDLV FT LQLMQECQEKLQQTFLIISHQIDALADFCDYRLVFADQQLKYVEE" FT RBS 1509..1514 FT CDS 1522..2175 FT /transl_table=11 FT /gene="orf2" FT /db_xref="GOA:Q48561" FT /db_xref="InterPro:IPR003339" FT /db_xref="UniProtKB/TrEMBL:Q48561" FT /protein_id="CAA91230.1" FT /translation="MNSSLKFILAFIISLEISLKASLTTNLIVIAFALIYLLVTRIKIK FT ELILLIAVPFIASFTIFATLFWFSPTPDAYYAWNLSTRVYVYTLTIACVTRNTTATDFA FT RSLEQNLHLPSKFAYGVLAAINIIPRMKTAVKQIRTSAMMRGMYLSFWSPVLYFKAILV FT ALNSADNLAQGMESHGYVEGQKRATIVAIPLTKKDWLIFFTLLILVNISLFIFK" FT RBS 2170..2176 FT CDS 2184..3068 FT /transl_table=11 FT /gene="pepI" FT /product="proline iminopeptidase" FT /function="hydrolyzes di/tripeptidases with proline in 1st FT position" FT /EC_number="3.4.11.5" FT /db_xref="GOA:P52278" FT /db_xref="InterPro:IPR000073" FT /db_xref="InterPro:IPR002410" FT /db_xref="InterPro:IPR005945" FT /db_xref="UniProtKB/Swiss-Prot:P52278" FT /protein_id="CAA91231.1" FT /translation="MEIIEGKMPFMGYETYYRIVGERSEKPPLVLLHGGPGSSHNYFEV FT LDELAQKDGRRIIMYDQLGCGESSIPDDHPELYTKETWVKELEALREHLALRKMHLLGQ FT SWGGMLAIIYMCDYHPEGIQSLILSSTLSSASLWSKELHRMIKYLPIEEQAAIHRAELT FT GNFNDPDYLKANEHFMNQHAIDMTKTWPECVMRKKRGGTVAYETAWGPNEYTPEGNLHD FT YEYTDKLSKIKVPTLITSGTDDLCTPYVAKTMQDQIASSKWRLFEGCGHMSFVEKTDEY FT VALLQEWLDQHDE" FT terminator 3073..3096 XX SQ Sequence 3240 BP; 1031 A; 608 C; 657 G; 944 T; 0 other; cagctgccat atgtccacct tccttaaatt aaacaaataa taatagtact ataagttcat 60 tataaaacaa aaagatattt agtattgtat ataattgcct tattgtactg aaatgaccct 120 ttttacggca gtagacaggc aaaaaatcat tttgtacaat cagaattatg atgatagaaa 180 ttagtgaatg taactttttc ttacgaaaaa gaacccctta tcaaccaact taatatgaat 240 attgcacccg gttttaatct cttgatcgga ccaacaggct gtggcaaatc taccctatta 300 aaaatcattg cgggactcta tccaaaatat gccggaaaat taacgggaaa aattgatctt 360 catggtcaaa aagcggccat gatgtttcaa aatgctgcgg aacaatttac gatgacaacg 420 ccacgtgaag aaatcatctt tgcgttagaa aatttgcaaa taaaagctaa ggattatgac 480 ctccacataa aaaaagcggt ggaattcacc aaaattgcag atttgcttga ccaaaaaatc 540 aatacactat caggtgggca acaacaacat gtagctctag ccgtcttaat tgcaatggat 600 gtcgatgttt ttctgctaga cgaacccttt gctagttgtg atcccaacac gcgtcatttt 660 ttgatagaaa aattagcaag tttagctgaa actggaagaa cgatcatatt aagcgaccac 720 gtattggatg attatgagaa gatttgtgat cacctatatc aatttgaagg taaaaccgtt 780 aaagaattat ctgctaatga aaaaaataaa ttgtttaagc aaaataaaca atttcatgaa 840 caatcttatt cttttgcgtt gccaactggc acacctgttt ttgaattaaa caagacacag 900 atcaaacaaa atcgtctttt gctcaaacaa aacaaattaa aaatctacgg caaaacaact 960 ctaattactg gttcaaatgg tgtcggcaaa acatcactat ttaaagcaat gactaaaatg 1020 attccgtaca aaggtaattt tacctattta gataatgaaa tttcaaagat aaaataccgt 1080 aaatatttat cacaaatagc acaatttttt caaaaggcaa gcgatcagtt tctaacagtt 1140 acagttaaag acgagattga attaagcaaa aaagatcgca ataatttttt cactgatgca 1200 aaaattgatg aatggctaga aaagttgcaa ctaaaacagc accttgatca ggtagtctat 1260 tctctatcag gtggccaaca aaaaaagctg caaattttgt taatgctgat gacaaagcat 1320 aatgtgttgc taattgacga gccattgagc ggactagatc acgaatcagt tgatttagtt 1380 ttgcagctga tgcaggaatg ccaagagaag ctccagcaga cgtttttgat cattagccat 1440 cagatagacg cgttagctga cttttgcgat tacaggctgg tttttgcgga tcaacagcta 1500 aaatatgtgg aggagtaagc catgaattct agtttaaagt ttattttggc ttttattatt 1560 tccctggaaa tatctttgaa ggcgagttta accaccaatt taatcgtcat tgcttttgcg 1620 ttgatttatc tcctggttac tagaatcaag atcaaggaat tgattttgtt aatcgccgtg 1680 ccatttattg cgtcgttcac gatatttgcg acgctgttct ggttttcacc gactcctgat 1740 gcttattatg cttggaatct gtcgacccgt gtgtatgtct atactttgac cattgcttgt 1800 gtcacgagga acaccaccgc gacagatttt gctcgttcgc ttgaacaaaa tctgcatcta 1860 cctagcaaat tcgcttatgg cgttttggcg gcaatcaaca ttattccgcg gatgaaaact 1920 gcagtgaagc agattcgtac atcagccatg atgcgcggaa tgtacctgag cttctggtca 1980 ccggtgctct actttaaggc aatcttggta gcgctcaact ctgccgacaa cttggcgcag 2040 ggcatggaat cccacggcta cgttgaagga cagaaaagag caaccattgt agcaattccg 2100 ctaactaaaa aagactggct gattttcttt actttattga ttttggttaa tatttcgttg 2160 ttcattttta agtaggcaaa aagatggaaa ttattgaagg aaaaatgccc ttcatgggct 2220 atgaaactta ttatcgaatt gttggtgaaa gaagcgagaa gccgccacta gttttgctgc 2280 acggcggacc tggctcaagc cacaattatt ttgaagtgct ggacgaatta gcacaaaaag 2340 atggccgccg catcattatg tatgaccaat taggttgcgg tgaaagcagc atccctgatg 2400 atcatcctga actttacact aaggaaactt gggtgaaaga attagaggct ttacgtgagc 2460 atttagcatt acgcaagatg catttgcttg gacaaagctg gggcgggatg ctcgccatta 2520 tttacatgtg cgactatcat ccagaaggta ttcagagctt gattctgtct tctaccctgt 2580 catcggcatc cctttggagt aaagaactcc accgcatgat taagtatttg ccaattgagg 2640 agcaggcagc gattcatagg gctgagttga ctggcaactt taacgatccg gattacctga 2700 aagctaacga gcatttcatg aaccagcatg ccattgatat gacgaagacc tggccggaat 2760 gcgtgatgcg caaaaagcgt ggcggcactg tggcatatga aacggcatgg ggaccgaacg 2820 agtatacacc agaaggcaat ctgcatgact atgagtacac ggataaattg agcaaaataa 2880 aagttccaac tctgattacg agcggaactg atgatttatg tacgccatat gtagctaaga 2940 ctatgcaaga tcagattgcc agcagcaaat ggcgtttgtt tgagggatgt ggccacatgt 3000 cctttgttga gaaaactgat gaatatgttg cactactaca ggagtggctg gatcaacatg 3060 atgaataata ataggctgac cgcgattggt cagctttttt gatgtaaata tgtttatctt 3120 tttaagacta aaacaaaaac gaaggagcaa tttctgcttc ctcgtctttc ttcattcttt 3180 cactgcgttt gtgagcctat ttgaagttca ctactttaac gtagcgtttg ttggttactg 3240 // |
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