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EBI DbfetchID Z50802; SV 3; linear; genomic DNA; STD; PRO; 3099 BP. XX AC Z50802; XX DT 17-AUG-1995 (Rel. 44, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 8) XX DE Escherichia coli dfrxiii and aadA4 genes for dihydrofolate reductase and DE adenyltransferase XX KW aadA4 gene; adenyltransferase; dfrxiii gene; dihydrofolate reductase; KW insertion element 26; integrase; IS26. XX OS Escherichia coli OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. XX RN [1] RP 1-1349 RX DOI; 10.1128/AAC.44.2.355-361.2000 RX PUBMED; 10639362. RA Adrian P.V., Thomson C.J., Klugman K.P., Amyes S.GB.; RT "New gene cassettes for trimethoprim resistance, dfr13, and RT Streptomycin-spectinomycin resistance, aadA4, inserted on a class 1 RT integron"; RL Antimicrob. Agents Chemother. 44(2):355-361(2000). XX RN [2] RC revised by [4] RA Adrian P.V.; RT ; RL Submitted (17-AUG-1995) to the EMBL/GenBank/DDBJ databases. RL Adrian P. V., University of Edinburgh, Medical Microbiology, Teviot Place, RL Edinburgh, UK, EH8 9AG XX RN [3] RA Adrian P.V.; RT ; RL Submitted (22-APR-1999) to the EMBL/GenBank/DDBJ databases. RL Adrian P. V., University of Edinburgh, Medical Microbiology, Teviot Place, RL Edinburgh, UK, EH8 9AG XX RN [4] RP 1-3099 RA Adrian P.V.; RT ; RL Submitted (20-JUL-1999) to the EMBL/GenBank/DDBJ databases. RL Adrian P. V., University of Edinburgh, Medical Microbiology, Teviot Place, RL Edinburgh, UK, EH8 9AG XX FH Key Location/Qualifiers FH FT source 1..3099 FT /organism="Escherichia coli" FT /isolate="RA33.2" FT /mol_type="genomic DNA" FT /db_xref="taxon:562" FT misc_feature complement(<1..573) FT /note="Class I integrase;allele:intI1" FT CDS 718..1215 FT /transl_table=11 FT /gene="dfrxiii" FT /product="dihydrofolate reductase" FT /function="trimethoprim resistance" FT /EC_number="1.5.1.3" FT /db_xref="GOA:Q59408" FT /db_xref="InterPro:IPR001796" FT /db_xref="InterPro:IPR012259" FT /db_xref="InterPro:IPR017925" FT /db_xref="UniProtKB/Swiss-Prot:Q59408" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA90683.1" FT /translation="MNPESVRIYLVAAMGANRVIGNGPDIPWKIPGEQKIFRRLTESKV FT VVMGRKTFESIGKPLPNRHTVVLSRQAGYSAPGCAVVSTLSHVSPSTAEHGKELYVARG FT AEVYALALPHANGVFLSEVHQTFEGDAFFPVLNAAEFEVVSSETIQGTITYTHSVYARR FT NG" FT CDS 1306..2094 FT /transl_table=11 FT /gene="aadA4" FT /product="adenyltransferase (AADA4)" FT /function="streptomycin resistance" FT /db_xref="GOA:Q9XB49" FT /db_xref="InterPro:IPR002934" FT /db_xref="UniProtKB/TrEMBL:Q9XB49" FT /protein_id="CAB41476.2" FT /translation="MGEFFPAQISEQLSHARGVIERHLAATLDTIHLFGSALDGGLKPD FT SDIDLLVTVSAAPNDSLRQALMLDLLKVSSPPGDGGPWRPLEVTVVARSEVVPWRYPAI FT RELQFGEWLRHDILSGTFEPSVLDHDLAILLTKARQHSLALLGPSAVTFFEPVPNEHFS FT KALFDTIAQWNSESDWKGDERNVVLALARIWYSASTGLIAPKDVAAAWVSERLPAEHRP FT IICKARAAYLGSEDDDLAMRVEETAAFVRYAKATIERILR" FT repeat_region complement(2130..2951) FT /function="recombination of IS26" FT /rpt_type=INVERTED FT /rpt_unit_range=2937..2951 FT /rpt_unit_range=2130..2144 FT /note="inverted repeat flanking IS26" FT CDS complement(2184..2888) FT /transl_table=11 FT /gene="tnp" FT /product="transposase (TNP)" FT /db_xref="GOA:Q57229" FT /db_xref="InterPro:IPR001584" FT /db_xref="InterPro:IPR012337" FT /db_xref="UniProtKB/TrEMBL:Q57229" FT /protein_id="CAB41477.1" FT /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS FT TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF FT YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR FT QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL FT GEMRLVSRVFEM" FT misc_feature <2951..>3099 FT /note="Tn3 resolvase;allele:tnpR" XX SQ Sequence 3099 BP; 681 A; 877 C; 856 G; 685 T; 0 other; ctgctcgcgc aggctgggtg ccaagctctc gggtaacatc aaggcccgat ccttggagcc 60 cttgccctcc cgcacgatga tcgtgccgtg atcgaaatcc agatccttga cccgcagttg 120 caaaccctca ctgatccgca tgcccgttcc atacagaagc tgggcgaaca aacgatgctc 180 gccttccaga aaaccgagga tgcgaaccac ttcatccggg gtcagcacca ccggcaagcg 240 ccgcgacggc cgaggtcttc cgatctcctg aagccagggc agatccgtgc acagcacctt 300 gccgtagaag aacagcaagg ccgccaatgc ctgacgatgc gtggagaccg aaaccttgcg 360 ctcgttcgcc agccaggaca gaaatgcctc gacttcgctg ctgcccaagg ttgccgggtg 420 acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct gttcggttcg 480 taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg 540 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttgtaca 600 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt 660 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt tagccgtatg 720 aacccggaat cggtccgcat ttatctggtc gctgccatgg gtgccaatcg ggttattggc 780 aatggtcccg atatcccctg gaaaatccca ggtgagcaga agatttttcg caggctcacc 840 gagagcaaag tggtcgttat gggccgcaag acatttgagt ccataggcaa gcccttacca 900 aaccgccaca cagtggtgct ctcgcgccaa gctggttata gcgctcctgg ttgtgcagtt 960 gtttcaacgc tgtcacacgt atcgccatcg acagccgaac acggcaaaga actctacgta 1020 gcgcgcggag ccgaggtata tgcgctggcg ctaccgcatg ccaacggcgt ctttctatct 1080 gaggtacatc aaacctttga gggtgacgcc ttcttcccag tgcttaacgc agcagaattc 1140 gaggttgtct catccgaaac cattcaaggc acaatcacgt acacgcactc cgtctatgcg 1200 cgtcgtaacg gctaacaagt ccgtcaacgg gacacccaaa tgctgcgcat ttgggttccc 1260 tccgctgcgc tccggcgccc gttacgtcca acgttaggca tcttcatggg tgaattcttt 1320 cctgcacaaa tttccgagca gctatcccac gctcgcgggg tgatcgagcg ccatctggct 1380 gcaacgctgg acacaatcca cctgttcgga tctgcgctcg atggagggtt gaagccggac 1440 agcgacatcg acttgctcgt gaccgtcagc gccgcaccta acgattcgct ccggcaggca 1500 ctaatgctcg acctgctaaa agtctcatca ccgccaggcg atggcggacc atggcgaccg 1560 ctggaggtga ctgttgtcgc tcgaagcgaa gtagtgccct ggcgctatcc ggcgatacgt 1620 gagcttcagt tcggtgagtg gctccgccac gacatcctct ccggaacgtt cgagccttcc 1680 gttctggatc acgatcttgc gattttgctg accaaggcga ggcaacacag ccttgctctg 1740 ctaggtccat ccgcagtcac gttcttcgag ccggtgccga acgagcattt ttccaaggcg 1800 cttttcgaca cgattgccca gtggaattca gagtcggatt ggaagggtga cgagcggaac 1860 gtcgttcttg ctcttgctcg catttggtac agtgcttcaa cgggtctcat tgctcctaag 1920 gacgttgctg ccgcatgggt atcggagcgt ttgcctgccg agcatcggcc catcatttgc 1980 aaggcacgcg cggcgtacct gggtagcgag gacgacgacc tagcaatgcg cgtcgaagag 2040 acggctgcgt tcgttcgata tgccaaagca acgattgaga gaatcttgcg ttgagcagca 2100 cgtgcgaaaa gtgcatcgac cggacccagg ggcactgttg caaagttagc gatgaggcag 2160 gccttttgtc ttattcaaag gccttacatt tcaaaaactc tgcttaccag gcgcatttcg 2220 cccaggggat caccataata aaatgctgag gcctggcctt tgcgtagtgc acgcatcacc 2280 tcaatacctt tgatggtggc gtaagccgtc ttcatggatt taaatcccag cgtggcgccg 2340 attatccgtt tcagtttgcc atgatcgcat tcaatcacgt tgttccggta cttaatctgt 2400 cggtgttcaa cgtcagacgg gcaccggcct tcgcgtttga gcagagcaag cgcgcgacca 2460 taggcgggcg ctttatccgt gttgatgaat cgcgggatct gccacttctt cacgttgttg 2520 aggattttac ccagaaaccg gtatgcagct ttgctgttac gacgggagga gagataaaaa 2580 tcgacagtgc ggccccggct gtcgacggcc cggtacagat acgcccagcg gccattgacc 2640 ttcacgtagg tttcatccat gtgccacggg caaagatcgg aagggttacg ccagtaccag 2700 cgcagccgtt tttccatttc aggcgcataa cgctgaaccc agcggtaaat cgtggagtga 2760 tcgacattca ctccgcgttc agccagcatc tcctgcagct cacggtaact gatgccgtat 2820 ttgcagtacc agcgtacggc ccacagaatg atgtcacgct gaaaatgccg gcctttgaat 2880 gggttcatgt gcagctccat cagcaaaagg ggatgataag tttatcacca ccgactattt 2940 gcaacagtgc cgccgatatg atccaactga taaaggaatt tgacgctcag ggcgtggcag 3000 tccggttcat tgatgacggg atcagtaccg acggtgatat ggggcaaatg gtggtcacca 3060 tcctgtcggc tgtggcacag gctgaacgcc ggaggatcc 3099 // |
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