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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   Z50095; SV 1; linear; mRNA; STD; FUN; 1434 BP.
XX
AC   Z50095;
XX
DT   18-NOV-1996 (Rel. 49, Created)
DT   18-APR-2005 (Rel. 83, Last updated, Version 6)
XX
DE   Agaricus bisporus mRNA for mannanase CEL4b (cel4 gene), strain D649
XX
KW   cel4 gene; CEL4b mannanase; mannosidase.
XX
OS   Agaricus bisporus
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Agaricomycetidae; Agaricales; Agaricaceae; Agaricus.
XX
RN   [2]
RP   1-1434
RA   Yague E.;
RT   ;
RL   Submitted (17-JUL-1995) to the EMBL/GenBank/DDBJ databases.
RL   Ernesto Yague, Experimental Pathology, United Medical And Dental School,
RL   GUY'S CAMPUS, LONDON BRIDGE, LONDON, SE1 9RT, UNITED, KINGDOM
XX
RN   [3]
RP   1-1434
RX   PUBMED; 9025297.
RA   Yague E., Mehak-Zunic M., Morgan L., Wood D.A., Thurston C.F.;
RT   "Expression of CEL2 and CEL4, two proteins from Agaricus bisporus with
RT   similarity to fungal cellobiohydrolase I and beta-mannanase, respectively,
RT   is regulated by the carbon source.";
RL   Microbiology (Reading, Engl.) 143:239-244(1997).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1434
FT                   /organism="Agaricus bisporus"
FT                   /strain="D649"
FT                   /mol_type="mRNA"
FT                   /dev_stage="young mycelia"
FT                   /clone_lib="lambda ZAP"
FT                   /clone="pEYc1200"
FT                   /tissue_type="mycelium"
FT                   /db_xref="taxon:5341"
FT   5'UTR           1..5
FT   CDS             6..1325
FT                   /gene="cel4"
FT                   /standard_name="beta-mannanase"
FT                   /product="CEL4b mannanase"
FT                   /function="endo-fashion hydrolysis of mannan and
FT                   heteromannan (by sequence comparison; no experimental
FT                   evidence)"
FT                   /EC_number="3.2.1.68"
FT                   /note="cellulose binding domain: 63..170"
FT                   /note="Expression of the gene induced (Northern analysis)
FT                   by crystalline cellulose. Repressed (Northern) by glucose,
FT                   fructose, lactose."
FT                   /note="Glycerol, mannitol, sorbitol, and maltose are
FT                   neutral carbon sources (Northern)"
FT                   /note="Catalytic domain (261..1325) belongs to family 5 of
FT                   glycosyl hydrolases (cellulase family A)"
FT                   /db_xref="GOA:Q92401"
FT                   /db_xref="HSSP:1QNR"
FT                   /db_xref="InterPro:IPR001547"
FT                   /db_xref="UniProtKB/TrEMBL:Q92401"
FT                   /protein_id="CAA90423.1"
FT                   /translation="MKPAIRFIILAISISLATADVPVWGQCGGRDWTGETACASGSSCV
FT                   VQNEWYSQCLPGSTTPTNPPPATTTSQTTAPPTTSHPVSTGFVKASGTRFTLNGQKYTV
FT                   VGGNSYWVGLTGLSTSAMNQAFSDIANAGGTTVRTWGFNEVTSPNGNYYQSWSGARPTI
FT                   NTGASGLLNFDNVIAAAKANGIRLIVALTNNWADYGGMDVYVNQMVGNGQPHDLFYTNT
FT                   AIKDAFKSYGRAFVSRYANEPTVMAWELANEPRCKGSTGTTSGTCTTTTVTNWAKEMSA
FT                   FIKTIDSNHLVAIGDEGFYNQPGAPTYPYQGSEGVDFEANLAISSVDFATFHSYPEPWG
FT                   QGADAKAWGTQWITDHAASMKRVNKPVILEEFGVTTNQPDTYAEWFNEIESSGLTGDLI
FT                   WQAGSHLSTGDTPNDGYAVYPDGPVYPLVKSHASAMKNRA"
FT   sig_peptide     6..62
FT                   /experiment="experimental evidence, no additional details
FT                   recorded"
FT   mat_peptide     63..1322
FT                   /product="CEL4b mannanase"
FT   3'UTR           1326..1434
XX
SQ   Sequence 1434 BP; 376 A; 352 C; 341 G; 365 T; 0 other;
     agaaaatgaa gcctgcgatt agattcatta ttttggctat ttctatctct ctagcgaccg        60
     cagatgttcc agtctggggt cagtgcggtg gcagggattg gacgggagag acagcttgtg       120
     caagtggatc atcttgtgtc gtgcagaacg aatggtactc gcagtgcctc cctggatcaa       180
     caactccaac aaaccctccc cctgccacta ccacctccca aactaccgct cctcctacca       240
     cttcacaccc agtgtcgacc ggatttgtca aagcttctgg taccagattc actctaaatg       300
     gccaaaaata caccgttgtt ggaggcaatt cctactgggt cggtttaact gggctgagca       360
     ccagtgcaat gaatcaggct ttctccgaca tcgctaacgc tggaggaaca accgtccgaa       420
     catggggatt caatgaagta acctcgccaa acggcaatta ttatcaatcc tggtctggtg       480
     ccagacctac tataaacacc ggagcgtctg gtctacttaa tttcgacaat gtgattgctg       540
     cggccaaggc aaatggaatt cgtctgatag ttgctctgac gaataattgg gcggactatg       600
     gtggcatgga cgtctatgtc aaccagatgg tgggcaatgg ccaaccacac gatcttttct       660
     acacaaacac cgcaatcaag gacgcgttca aatcttatgg ccgggccttc gtgagccgct       720
     atgccaacga acctaccgta atggcttggg agcttgcaaa tgagccgagg tgcaaaggta       780
     gcaccggcac gacttctgga acctgtacaa ctacgaccgt cacgaattgg gccaaggaga       840
     tgtcggcgtt tattaaaacc attgatagca accatctcgt tgctattggt gatgagggtt       900
     tctataatca acccggtgct ccaacttatc cataccaagg aagcgaaggt gttgatttcg       960
     aagctaatct cgctatctcc tctgtcgact ttgcgacctt ccattcctat ccggaaccgt      1020
     ggggacaggg cgccgatgca aaagcctggg gcactcaatg gattacagat catgctgcca      1080
     gtatgaagag ggtcaacaaa cccgttatcc ttgaagaatt cggtgtaact actaatcaac      1140
     ctgatactta cgctgagtgg ttcaacgaga tagaatcatc agggttgact ggtgatctca      1200
     tttggcaagc cggttctcac ttgtctacgg gagatacacc caatgacggt tatgctgtct      1260
     accccgatgg tccagtatac ccgttagtga agtcgcatgc aagtgcaatg aaaaaccggg      1320
     cttgaattag tattgttgat cacgattgaa ttgtatgtac ttgtattcca tatccgtttt      1380
     tgttgaaagt agtacacatg cgtacatacc ggtaaataaa cactttgaat tgtg            1434
//
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