EBI Dbfetch

ID   Z50094; SV 1; linear; mRNA; STD; FUN; 1851 BP.
XX
AC   Z50094;
XX
DT   18-NOV-1996 (Rel. 49, Created)
DT   18-APR-2005 (Rel. 83, Last updated, Version 5)
XX
DE   A.bisporus mRNA for exocellobiohydrolase.
XX
KW   1-4 beta cellobiohydrolase; cellobiohydrolase; Exocellobiohydrolase.
XX
OS   Agaricus bisporus
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Agaricomycetidae; Agaricales; Agaricaceae; Agaricus.
XX
RN   [1]
RP   1-1851
RX   PUBMED; 9025297.
RA   Yague E., Mehak-Zunic M., Morgan L., Wood D.A., Thurston C.F.;
RT   "Expression of CEL2 and CEL4, two proteins from Agaricus bisporus with
RT   similarity to fungal cellobiohydrolase I and beta-mannanase, respectively,
RT   is regulated by the carbon source.";
RL   Microbiology (Reading, Engl.) 143:239-244(1997).
XX
RN   [2]
RP   1-1851
RA   Yague E.;
RT   ;
RL   Submitted (17-JUL-1995) to the EMBL/GenBank/DDBJ databases.
RL   Ernesto Yague, Experimental Pathology, United Medical And Dental School,
RL   GUY'S CAMPUS, LONDON BRIDGE, LONDON, SE1 9RT, UNITED, KINGDOM
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1851
FT                   /organism="Agaricus bisporus"
FT                   /strain="D649"
FT                   /mol_type="mRNA"
FT                   /dev_stage="young mycelia"
FT                   /clone_lib="lambda ZAP"
FT                   /clone="pEYc501, pEYc505"
FT                   /db_xref="taxon:5341"
FT   5'UTR           1..12
FT   CDS             13..1533
FT                   /gene="cellulose-growth-specific (cel)2"
FT                   /product="Exocellobiohydrolase (1-4 beta
FT                   cellobiohydrolase)"
FT                   /function="Hydrolysis of beta 1-4 D glycosidic linkages in
FT                   cellulose releasing cellobiose from the non-reducing end of
FT                   the chain"
FT                   /EC_number="3.2.1.91"
FT                   /note="67..1389 Catalytic domain. It belongs to family 7 of
FT                   glycosyl hydrolases"
FT                   /note="1390..1434 Proline/Serine/Threonine-rich linker"
FT                   /note="1435..1530 fungal cellulose-binding domain"
FT                   /note="/Expression of the gene induced (Northern analysis)
FT                   by crystalline cellulose. Repressed (Northern) by glucose,
FT                   fructose, and lactose."
FT                   /note="Glycerol, mannitol, sorbitol, and maltose are
FT                   neutral carbon sources (Northern analysis)"
FT                   /db_xref="GOA:Q92400"
FT                   /db_xref="InterPro:IPR000254"
FT                   /db_xref="InterPro:IPR001722"
FT                   /db_xref="InterPro:IPR008985"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q92400"
FT                   /citation=[1]
FT                   /protein_id="CAA90422.1"
FT                   /translation="MFPRSILLALSLTAVALGQQVGTNMAENHPSLTWQRCTSSGCQNV
FT                   NGKVTLDANWRWTHRINDFTNCYTGNEWDTSICPDGVTCAENCALDGADYAGTYGVTSS
FT                   GTALTLKFVTESQQKNIGSRLYLMADDSNYEIFNLLNKEFTFDVDVSKLPCGLNGALYF
FT                   SEMAADGGMSSTNTAGAKYGTGYCDSQCPRDIKFIDGEANSEGWEGSPNDVNAGTGNFG
FT                   ACCGEMDIWEANSISSAYTPHPCREPGLQRCEGNTCSVNDRYATECDPDGCDFNSFRMG
FT                   DKSFYGPGMTVDTNQPITVVTQFITDNGSDNGNLQEIRRIYVQNGQVIQNSNVNIPGID
FT                   SGNSISAEFCDQAKEAFGDERSFQDRGGLSGMGSALDRGMVLVLSIWDDHAVNMLWLDS
FT                   DYPLDASPSQPGISRGTCSRDSGKPEDVEANAGGVQVVYSNIKFGDINSTFNNNGGGGG
FT                   NPSPTTTRPNSPAQTMWGQCGGQGWTGPTACQSPSTCHVINDFYSQCF"
FT   sig_peptide     13..66
FT   mat_peptide     67..1530
FT                   /product="cellobiohydrolase"
FT   3'UTR           1534..1851
XX
SQ   Sequence 1851 BP; 434 A; 496 C; 447 G; 474 T; 0 other;
     ccagaactca acatgttccc gagatccatt ctcctcgccc tctccctcac tgctgttgcc        60
     ttgggccagc aagtcggtac caacatggcc gaaaaccacc catcgctcac ttggcaaagg       120
     tgcaccagta gtggttgcca gaacgtgaac ggcaaagtca ccctcgacgc taactggcgt       180
     tggactcacc gcatcaacga cttcaccaac tgctacactg gtaacgaatg ggatacttct       240
     atttgtcctg acggcgtaac ttgtgccgag aactgtgcgc tcgatggtgc tgactacgca       300
     ggcacatacg gtgtcacctc ctccggtacc gctcttactc tgaaattcgt cactgagagc       360
     cagcagaaga acattggttc tcgtctctac ctcatggctg atgattccaa ctatgagatc       420
     ttcaacctct tgaacaaaga gttcactttc gacgtcgatg tctccaaact tccttgcggt       480
     cttaacggtg ccctttactt ttcggagatg gctgcagatg gtggcatgtc gtcgaccaat       540
     acggctggtg ccaagtacgg tactggttat tgtgactcgc aatgccctcg tgacatcaag       600
     tttatcgacg gagaggccaa cagcgagggt tgggaaggct ccccgaacga cgtcaacgct       660
     ggaaccggta actttggtgc ctgctgcggt gaaatggata tctgggaggc caactctatc       720
     tcttccgcct acactcccca tccttgtagg gaacccggac tccagcgttg cgagggtaac       780
     acttgctcag tcaacgatcg ctatgcgact gagtgtgacc ccgacggttg tgatttcaac       840
     tccttccgta tgggagacaa gtcgttctac ggccccggca tgacagtcga caccaaccag       900
     cccatcactg tcgtcactca gtttatcact gataatggct ccgacaacgg taacctccag       960
     gagatccgcc gcatctacgt ccagaacggc caggtcatcc agaactccaa tgtcaacatt      1020
     cccggaatcg actccggcaa ctccatttcc gcagaattct gtgatcaggc taaggaagcc      1080
     ttcggtgatg agcgatcatt ccaagatagg ggtggattgt cgggtatggg ttctgcgttg      1140
     gaccgcggaa tggttctcgt cctttccatc tgggatgacc atgccgttaa catgttgtgg      1200
     ctcgactctg actaccccct tgacgcctcc ccaagtcaac caggtatctc ccgtggtacc      1260
     tgctctaggg actctggtaa acccgaggat gttgaggcta acgctggcgg cgtccaagtc      1320
     gtctactcca atatcaagtt cggtgacatc aactccactt tcaacaacaa cggtggtggc      1380
     ggtggcaacc ccagcccaac taccactcgc cccaactccc ccgctcaaac catgtggggt      1440
     caatgtggtg gtcaaggctg gactggcccg actgcttgcc aatctccatc tacttgccac      1500
     gtcatcaatg acttctatag ccaatgtttc tgagtagctt gagaatgttt atttagcaat      1560
     accttggtag tacctcgacg gtaaccgggc gtttttgtaa aaaaaaatgc gattatgtgt      1620
     atatgtaaaa tttcgataga aataactttt gcatcgcttt gctttagtgc cttttttgtt      1680
     ttgataagct taagttctct tttttttgag gggagagaaa gtgagatagt ttttagtgaa      1740
     tcagtgaccg aatgagggat aaaacgggga gtactattcg atgattatta gctacgacat      1800
     ggattgtatt tatactcaag aaataatgtc caggcgtcgg gtggcgaaag c               1851
//