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EBI DbfetchID Z49992; SV 1; linear; genomic DNA; STD; PRO; 6863 BP. XX AC Z49992; XX DT 04-JUL-1995 (Rel. 44, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 12) XX DE B.subtilis celA, celB, celC, celD and ywaA genes XX KW 6-phospho-beta-glucosidase; cel operon regulator; celA gene; celB gene; KW celC gene; celD gene; cellobiose phosphotransferase. XX OS Bacillus subtilis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-6863 RX PUBMED; 8990303. RA Tobisch S., Glaser P., Kruger S., Hecker M.; RT "Identification and characterization of a new beta-glucoside utilization RT system in Bacillus subtilis"; RL J. Bacteriol. 179(2):496-506(1997). XX RN [2] RP 1-6863 RA Glaser P.; RT ; RL Submitted (03-JUL-1995) to the EMBL/GenBank/DDBJ databases. RL Philippe Glaser, Regulation de l'Expression Genetique, Institut, Pasteur, RL 28 Rue du Dr Roux, Paris, Paris, 75724, France XX FH Key Location/Qualifiers FH FT source 1..6863 FT /organism="Bacillus subtilis" FT /strain="168" FT /mol_type="genomic DNA" FT /db_xref="taxon:1423" FT terminator 10..40 FT RBS 173..179 FT /gene="celR" FT CDS 187..2112 FT /transl_table=11 FT /gene="celR" FT /product="putative cel operon regulator" FT /db_xref="GOA:P46321" FT /db_xref="InterPro:IPR002178" FT /db_xref="InterPro:IPR007737" FT /db_xref="InterPro:IPR011608" FT /db_xref="InterPro:IPR013011" FT /db_xref="InterPro:IPR013196" FT /db_xref="InterPro:IPR016152" FT /db_xref="UniProtKB/Swiss-Prot:P46321" FT /protein_id="CAA90284.1" FT /translation="MLHGRLRDILRLLMAAEAPVTSSFFAAQLNVTTRTVRNDIKELQG FT VLSGHGAFVQSVRGSGYKLRIDDEQVFRTLLQDEFQQKKGLPVLPEERMAYLMKRLLLA FT DHYLKLDELAEELFISKSTLQTDLKEVKKRLLPYRIVMETRPNYGFKLRGDEVQMRYCM FT AEYIVDERETEIDVLNEKADILPKEEIEIIRSAILKKMKNDRIPLSNMGLNNLIIHIAI FT ACKRIRTENYVSLFPKDMDHILHQKEYQAAEAIVKELESKLSVTFPKDETAYITMHLLG FT TKRMTQSQCGEDTFSIEEETDQLTLAMIKAVDRELKLGILHDKELKIGLALHMKPAISR FT NRYGMNLRNPMLAAIKEHYPLAFEAGIIAGIVIKEQTGIEIHENEIGYLALHFGAAIER FT KKTESPPKRCIIVCASGAGSAQLLREKLRSHFGKRLDILGTAEYYSLDQMSYESIDFVI FT STIPIKKELPVPVLKVNTILGGTDFTKIESILSDEKEKANRYLKKELVFFQEDLRSKEE FT VIQFLGQKVVECGFADEEIIDSIFEREDMSPTCFGNLVAIPHPLVPQTKTTFWAVCTLK FT KPIDWESQRVQFVCLLCVEKENKADLQSMYKLLGSILDDPAAMNQLIKCRSYQELSDVF FT DQKMLS" FT stem_loop 2129..2150 FT stem_loop 2161..2180 FT RBS 2226..2232 FT /gene="celA" FT CDS 2240..2548 FT /transl_table=11 FT /gene="celA" FT /product="putative cellobiose phosphotransferase enzyme FT II'" FT /db_xref="GOA:P46318" FT /db_xref="InterPro:IPR003501" FT /db_xref="InterPro:IPR013012" FT /db_xref="InterPro:IPR014350" FT /db_xref="UniProtKB/Swiss-Prot:P46318" FT /protein_id="CAA90285.1" FT /translation="MNILLVCAAGMSTSLLVSKMEKSAQEQGKDYTIWAVSGDSVQNHI FT DKADVLLLGPQVRYMLPQLKKLGESKGVPVDVINTVHYGTCNGAEVLKSAEQLGHVS" FT RBS 2548..2555 FT /gene="celB" FT CDS 2564..3922 FT /transl_table=11 FT /gene="celB" FT /product="putative cellobiose phosphotransferase enzyme FT II''" FT /note="gtg start codon" FT /db_xref="GOA:P46317" FT /db_xref="InterPro:IPR003352" FT /db_xref="InterPro:IPR004501" FT /db_xref="InterPro:IPR004796" FT /db_xref="UniProtKB/Swiss-Prot:P46317" FT /protein_id="CAA90286.1" FT /translation="MNKVNQILEEKVMPIAGRIAGQRHLQALRDGIILTMPLIIIGSFF FT LIIGNLPIPGYAEFMAKTFGSSWSEKLAYPVDATFEIMGLVAAFGIAYRLAEKYGVDAL FT SAGAISLAAFLLATPYQVPFMPDGATKEIMVGGGIPLSLMGSKGLFVAMIIAMVSTEIY FT RLIIQRNLVFKMPDGVPPAVSKSFVALIPGFAVIFLIWAARLIVEATPFESLHNIVSVL FT LGTPLSILGGSLGGSLVAEAVKMLLWACGLHGANIVGGVMAPIWYGAMDANRIAFQAGE FT ELPKIFTQQFFDIWVNIGGSGATLALVVTMFLRARSKQMKQLGKLAVGPAIFNINEPII FT FGMPIVMNPMLLLPFIITPLVTVTLTYIGMSTGLVAKPAGIAVPWTMPPIFSGYLATGG FT KVSGAVMQAINIAVSFVVYYPFFRMWDKQKLKEENDLELVQTPAATDDKEAAL" FT RBS 3926..3934 FT /gene="celC" FT CDS 3941..4273 FT /transl_table=11 FT /gene="celC" FT /product="putative cellobiose phosphotransferase enzyme FT III" FT /note="gtg start codon" FT /db_xref="GOA:P46319" FT /db_xref="InterPro:IPR003188" FT /db_xref="UniProtKB/Swiss-Prot:P46319" FT /protein_id="CAA90287.1" FT /translation="MNEEMEQIIFQIILHGGNGRSSAMEAIAAAKSGDAEEARKKLQDA FT AEELSKAHHYQTELIQNEAGGEKTEMTLLMVHAQDHLMNAMTVKDMAAEIIELYEKITE FT QRGASI" FT RBS 4255..4262 FT /gene="celD" FT CDS 4270..5598 FT /transl_table=11 FT /gene="celD" FT /product="putative 6-phospho-beta-glucosidase" FT /db_xref="GOA:P46320" FT /db_xref="InterPro:IPR001088" FT /db_xref="InterPro:IPR015955" FT /db_xref="InterPro:IPR016040" FT /db_xref="InterPro:IPR019802" FT /db_xref="UniProtKB/Swiss-Prot:P46320" FT /protein_id="CAA90288.1" FT /translation="MTKGLKIVTIGGGSSYTPELVEGFIKRYDELPVRELWLVDIPEGE FT EKLNIVGTLAKRMVEKAGVPIDIHLTLDRRKALKDADFVTTQFRVGLLQARAKDERIPL FT KYGVIGQETNGPGGLFKGLRTIPVILEIAKDIEELCPNAWLVNFTNPAGMVTEALLRYS FT NLKKVVGLCNVPIGIKMGVAKALDVDVDRVEVQFAGLNHMVFGLDVFLDGVSVKEQVIE FT AMGDPKNAMTMKNISGAEWEPDFLKALNVIPCGYHRYYFKTKEMLEHELEASQTEGTRA FT EVVQKVEKELFELYKDPNLAIKPPQLEKRGGAYYSDAACNLISSIYNDKHDIQPVNTIN FT NGAIASIPDDSAVEVNCVMTKTGPKPIAVGDLPVSVRGLVQQIKSFERVAAEAAVTGDY FT QTALLAMTINPLVPSDTVAKQILDEMLEAHKAYLPQFFNKIEA" FT terminator 5597..5630 FT CDS complement(5632..6723) FT /transl_table=11 FT /gene="ywaA" FT /product="Unknown, highly similar to yeast TWT1 protein and FT to Mouse ECA39 protein, similar to amino acid FT aminotransferases " FT /db_xref="GOA:P39576" FT /db_xref="InterPro:IPR001544" FT /db_xref="InterPro:IPR005786" FT /db_xref="InterPro:IPR018300" FT /db_xref="UniProtKB/Swiss-Prot:P39576" FT /protein_id="CAA90289.1" FT /translation="MTKQTIRVELTSTKKPKPDPNQLSFGRVFTDHMFVMDYAADKGWY FT DPRIIPYQPLSMDPTAMVYHYGQTVFEGLKAYVSEDDHVLLFRPEKNMERLNQSNDRLC FT IPQIDEEQVLEGLKQLVAIDKDWIPNAEGTSLYIRPFIIATEPFLGVAASHTYKLLIIL FT SPVGSYYKEGIKPVKIAVESEFVRAVKGGTGNAKTAGNYASSLKAQQVAEEKGFSQVLW FT LDGIEKKYIEEVGSMNIFFKINGEIVTPMLNGSILEGITRNSVIALLKHWGLQVSERKI FT AIDEVIQAHKDGILEEAFGTGTAAVISPVGELIWQDETLSINNGETGEIAKKLYDTITG FT IQKGAVADEFGWTTEVAALTESK" FT RBS complement(6730..6736) FT /gene="ywaA" XX SQ Sequence 6863 BP; 1877 A; 1555 C; 1676 G; 1755 T; 0 other; ggatcctcta gaacagccgg ctgatcccgg ctgttttttt ataggtcagt tttccctcta 60 gtcctccgga tgttcattga cttgccattc tctccattat agaatttata tacatttcgc 120 atcccttcat catcctttct gcctattcac tgaatccctg catctactag ttaggaggtt 180 tttctcatgc ttcatgggcg actacgtgat attctgcgct tgctgatggc ggctgaagcc 240 ccggtgacaa gttcgttttt tgccgcgcaa ttaaacgtaa cgacgcggac agtccgcaat 300 gatataaagg aacttcaagg tgtgctaagc ggccacggtg cctttgtaca atctgtaaga 360 ggctccggct acaagctccg cattgatgac gagcaagtat tcagaacact gctgcaagat 420 gaatttcagc aaaaaaaggg cctgccggta ttgccggagg agagaatggc ctatcttatg 480 aaacgtctgc tgctggcgga ccattatctc aagctcgatg aacttgctga agaactattt 540 atctcaaaat ctacactcca aacggattta aaggaagtaa agaaacgtct gctgccttac 600 cgcatcgtca tggaaacacg tccgaattac ggcttcaagc tgcgcgggga cgaggtgcag 660 atgaggtact gcatggcgga gtacattgtt gatgaaagag aaacggagat agatgtttta 720 aatgaaaagg ctgatatttt gccaaaagaa gaaattgaga tcatccgttc cgccatcttg 780 aaaaaaatga aaaacgaccg catccctctg tccaacatgg gactgaataa cctcattatc 840 catatcgcta tcgcctgtaa acggatcaga acggaaaact atgtttctct tttcccgaaa 900 gatatggatc atatcctgca tcaaaaggag taccaggctg cagaagccat cgtaaaagag 960 ctggaatcaa agctttccgt tacgtttccg aaagacgaga cggcctacat caccatgcat 1020 ctccttggca caaaacgaat gacacaatca caatgcggag aggacacatt ttcaatagaa 1080 gaggaaaccg atcagctgac acttgccatg atcaaagctg ttgatcgtga actgaagctt 1140 ggcatcctgc atgacaaaga gctgaaaatc ggcttggcac tgcatatgaa gccggcgatc 1200 agccgcaacc gatacggcat gaatcttcga aatccgatgc tggcagcgat caaggaacat 1260 tatccactcg cttttgaagc cgggatcatt gccggaatcg tgataaagga acaaacgggt 1320 attgagattc atgaaaacga aattggatat ttggctctcc actttggcgc cgcgattgaa 1380 cgaaaaaaaa cagaaagccc gccgaaacgc tgtatcattg tatgcgcatc gggagccgga 1440 agcgcccagc ttctgcgcga aaaactgcgt tcccatttcg gcaagcggct cgatattctt 1500 ggaacggctg agtattatag tcttgatcaa atgtcctacg aatcaattga ttttgtcatc 1560 agcaccattc cgattaaaaa agagctccct gtaccagtac tgaaggttaa caccatctta 1620 ggcggaactg actttacaaa aatagaatct attctgagtg atgaaaagga aaaagccaat 1680 cgctacctaa aaaaagaact cgtttttttt caggaggatc tgcgctcaaa agaagaagtg 1740 attcaatttt tagggcagaa agtggtcgaa tgcggctttg ccgatgaaga aatcatagat 1800 tccatttttg aaagagaaga catgtcgcca acgtgttttg gcaatcttgt tgccatcccg 1860 cacccgcttg ttccgcagac gaaaacaacg ttttgggccg tttgtacgct caaaaaaccg 1920 atcgattggg agagtcagcg agttcaattt gtctgtctgc tttgtgtcga aaaggagaac 1980 aaagcggatt tacaaagcat gtataaactg cttggcagca ttttggatga cccggcggcg 2040 atgaaccagc tgatcaaatg ccgttcatat caagagcttt cggatgtgtt tgaccaaaaa 2100 atgctgagct gacagcctgt atataccttt tttccgttgc tgcggaaaaa ccctgagtgt 2160 ttatgaaagc gatttcataa tatgatgata gcaacagcag gacattacaa aattgatcaa 2220 actctagggg gtcttttaca tgaatatatt actcgtttgt gcagcaggca tgtctactag 2280 cttactggtg agcaagatgg aaaaaagtgc gcaggaacag ggaaaagact ataccatttg 2340 ggcagtttca ggcgattctg tacaaaatca tatcgataaa gcggatgtat tgcttctcgg 2400 tccgcaagtc cgttacatgc ttccgcagct caaaaaatta ggagagtcaa agggggttcc 2460 ggtagacgtc attaataccg tccattatgg aacgtgcaat ggggcggaag tgctgaaatc 2520 agctgaacag cttggacatg tgtcatagag aggggcgggc tatgtgaata aagtcaatca 2580 gatcttagaa gaaaaggtca tgcctatagc gggaagaatt gcgggacagc gtcatttgca 2640 ggcgctgcgt gatggtatca tcctcacgat gcccctgatt atcatcggtt cctttttcct 2700 catcattggc aacctcccga tcccgggata cgctgagttt atggcaaaaa cgtttggcag 2760 ctcctggtct gaaaaactgg cctacccagt cgacgcgacc tttgaaatta tgggtcttgt 2820 cgccgcgttc ggcattgcct accggctcgc cgagaaatac ggtgttgatg ccttgtcggc 2880 cggagccatt tcccttgcgg catttttatt agcaacacct tatcaagtgc cgtttatgcc 2940 ggatggcgcg acgaaagaaa tcatggttgg cggcgggatt ccgctttcgc tcatgggaag 3000 caaaggctta tttgtcgcca tgattatcgc aatggtgtcc acagagattt accgccttat 3060 tatccagcgc aatttagtct tcaaaatgcc ggacggtgtc ccgccggcgg taagcaagtc 3120 atttgtcgcg ttaatccccg gctttgccgt catcttccta atttgggcag cccggctgat 3180 tgttgaagcg acaccgtttg aaagccttca taatattgta agcgttttat tggggactcc 3240 gctttccatt ctgggaggaa gccttggagg aagccttgtc gccgaagccg tcaaaatgct 3300 tttatgggcc tgcggccttc acggtgcaaa cattgttggc ggcgtcatgg caccgatttg 3360 gtacggcgca atggatgcca accggatcgc attccaagcc ggtgaggagc ttcctaagat 3420 tttcacccag caatttttcg atatctgggt caatattggc ggaagcggcg ccacattggc 3480 actggttgtc acgatgttcc tgcgcgcccg aagcaaacaa atgaagcagc tcgggaagct 3540 tgcggtcggc ccggccatct tcaatattaa cgaaccgatt atattcggaa tgccgatcgt 3600 catgaaccca atgctcttac ttccgtttat catcacgccg cttgtcacag tgacactcac 3660 gtacatcgga atgagcaccg gccttgtcgc caaaccggcg ggtatcgcag tgccgtggac 3720 catgccgccg atcttttcag gctacttggc gacaggagga aaagtctcag gcgcggtcat 3780 gcaagccatt aatattgccg tgtcctttgt tgtctactat ccattcttca gaatgtggga 3840 taagcagaag ctgaaagaag aaaacgatct ggagctggtc caaacgcctg cagcaacaga 3900 tgacaaagaa gcggctctgt agagaaagga agtgaatcgc gtgaatgagg aaatggaaca 3960 aatcattttt caaatcatcc ttcacggagg aaacggtagg agctccgcaa tggaggccat 4020 tgccgctgcg aaaagcggtg acgcagaaga agcgcggaaa aagcttcaag acgctgctga 4080 agaattgtca aaagcccatc attatcagac agagctgatc caaaatgaag cgggcggaga 4140 aaagacggaa atgacgcttc tgatggttca tgcacaggat catttaatga atgcaatgac 4200 agtgaaggat atggctgcag agatcattga gttatatgaa aaaatcaccg agcaaagggg 4260 agcaagcata tgacaaaagg attgaagatt gtaacgattg gcggaggttc aagctatacg 4320 cccgagctag tggaggggtt cattaaacgg tatgatgagc tgcctgtccg tgaattatgg 4380 cttgtcgata tccccgaggg cgaagaaaag ctgaatattg tcggcacact cgccaaacgg 4440 atggttgaaa aagctggcgt cccgattgac attcatctta cgctcgaccg gagaaaggcg 4500 ctgaaagacg cagactttgt cacgacccaa ttccgcgtcg gacttttgca ggcaagagca 4560 aaggatgagc gcattccgct aaaatacgga gtgataggcc aggaaacaaa cggcccggga 4620 ggtctgttta aaggattgcg cacaattccg gtcatccttg agatcgcaaa ggacatagaa 4680 gagctttgcc cgaacgcttg gcttgttaac ttcacaaacc ctgccggcat ggtaacagaa 4740 gccttgcttc gctactccaa cctgaagaaa gtcgtcggtc tttgcaatgt tccaatcggc 4800 atcaaaatgg gcgtagccaa agcgcttgat gtagatgtgg accgcgtaga agtccaattt 4860 gccggactga accatatggt attcgggctg gatgttttct tggacggcgt aagtgtgaag 4920 gagcaagtca tcgaggcgat gggcgacccg aaaaacgcga tgacgatgaa aaatatctca 4980 ggcgccgaat gggaaccgga tttcttaaaa gcgctcaatg tcatcccttg cggctatcac 5040 cgttactatt ttaaaacaaa agagatgctg gagcacgaac tggaagcatc acaaacggag 5100 ggcacccgcg ctgaagtcgt acagaaggtc gaaaaagagc tgttcgagct ttataaagat 5160 ccaaaccttg cgatcaaacc gccgcagctg gaaaaacggg gcggagctta ctacagtgat 5220 gcagcatgta acttaatcag ctccatttat aatgataaac atgacattca gccggtgaac 5280 acgatcaata atggtgcaat agcgagtatt ccagacgatt ccgcagttga agtgaactgt 5340 gtgatgacga aaaccggccc taagccaatt gctgtcggcg acttgccggt gtctgtccgc 5400 ggccttgtcc agcaaatcaa atcctttgaa cgtgtcgcgg cagaagcggc agttacaggt 5460 gactatcaaa ctgcgctcct tgccatgacg attaacccgc ttgtcccgtc cgatactgtg 5520 gccaaacaga ttttagacga aatgctagag gcgcacaaag cgtatctgcc gcagtttttc 5580 aacaaaattg aagcgtaaaa aaagccggcc tgtaatgggc cggctttttt cttacttgct 5640 ttcagtcagc gctgcgactt cggtcgtcca tccgaattcg tctgcgacag cgcctttttg 5700 aatgcctgta atcgtgtcat atagtttttt tgcgatttct cctgtttcac cgttgttgat 5760 cgaaagtgtt tcatcctgcc agatcagctc gccgactggg gaaataacag ctgctgtacc 5820 tgttccgaag gcttcttcca ggatgccgtc tttatgggct tggatgacct catcgatcgc 5880 aatttttcgt tctgaaactt gaaggcccca atgcttaagc aaggcgatga ctgaattgcg 5940 cgtaatgcct tccaggatgc tcccgttcag catcggtgtt acgatttcac cgttgatttt 6000 gaagaagatg ttcatgcttc cgacttcttc gatgtatttc ttctcaatgc cgtccagcca 6060 gagtacttga gaaaatcctt tctcttcggc tacctgctgc gcttttaagc ttgaagcata 6120 gtttcctgcg gttttggcat ttcctgttcc gccttttacc gcacggacaa attcactttc 6180 aacagcgatt ttgaccggct taatgccttc tttgtaataa gagccgaccg gagaaagaat 6240 gatcaagagc ttatacgtat gagatgccgc aacaccaagg aaaggctcgg ttgcgatgat 6300 gaacggacgg atgtaaaggg acgtgccctc cgcatttgga atccagtctt tatcaattgc 6360 gacaagctgc tttaagcctt caagaacctg ttcttcatca atttgcggga tgcagaggcg 6420 gtcgtttgat tgattcaggc gttccatatt tttttccggt ctgaaaagca gaacatggtc 6480 atcctctgac acgtaagcct ttaacccttc aaacacggtt tggccgtagt gatagaccat 6540 tgcagttgga tccattgata agggttgata aggaatgatt cttggatcgt accaaccttt 6600 atctgcggca tagtccatta caaacatgtg gtctgtaaac actcttccga acgaaagctg 6660 atttgggtct ggtttcggtt tttttgttga tgtcaattca acgcgaattg tttgtttagt 6720 catggtcaat ctccctgctg ttaaattgtt tatattatca aacttatttg aaaataatta 6780 tattctattg tataccttca acaaataatc gcaataggtt tgctcaaatt tgtaagaaat 6840 tcatatgaaa aaagcaggag aaa 6863 // |
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