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EBI DbfetchID Z49961; SV 1; linear; genomic DNA; STD; PRO; 3015 BP. XX AC Z49961; XX DT 31-DEC-1995 (Rel. 46, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 20) XX DE T.neapolitana DNA for uxuA, xp148, and xynB genes XX KW D-mannonate hydrolase; X protein; xylanase. XX OS Thermotoga neapolitana OC Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga. XX RN [1] RP 1-3015 RX PUBMED; 9113732. RA Velikodvorskaya T., Volkov I., Vasilevko V., Zverlov V., Piruzian E.; RT "Purification and some properties of Thermotoga neapolitana thermostable RT xylanase B expressed in E. coli cells"; RL Biochemistry Mosc. 62(1):66-70(1997). XX RN [2] RC (sites) RX DOI; 10.1006/bbrc.1993.1974 RX PUBMED; 8352795. RA Dakhova O., Kurepina N., Zverlov V., Svetlichnyi V., Velikodvorskaya G.; RT "Cloning and expression in Escherichia coli of Thermotoga neapolitana genes RT coding for enzymes of carbohydrate substrate degradation"; RL Biochem. Biophys. Res. Commun. 194(3):1359-1364(1993). XX RN [3] RP 1-3015 RA Zverlov V.V.; RT ; RL Submitted (28-JUN-1995) to the EMBL/GenBank/DDBJ databases. RL Zverlov V. V., Institute of Molecular Genetics, Russian Academy of RL Sciences, Kurchatov Sq.46, Moscow, Russia, 123182 XX FH Key Location/Qualifiers FH FT source 1..3015 FT /organism="Thermotoga neapolitana" FT /strain="Z2706-MC24" FT /mol_type="genomic DNA" FT /clone="pTT32" FT /db_xref="taxon:2337" FT RBS 193..197 FT /citation=[1] FT CDS 206..1288 FT /transl_table=11 FT /gene="uxuA" FT /product="D-mannonate hydrolase" FT /function="D-mannonate = 2-dehydro-3-deoxy-D-gluconate + FT H(2)O" FT /EC_number="4.2.1.8" FT /note="putative" FT /db_xref="GOA:Q60040" FT /db_xref="InterPro:IPR004628" FT /db_xref="InterPro:IPR013022" FT /db_xref="UniProtKB/Swiss-Prot:Q60040" FT /citation=[1] FT /protein_id="CAA90233.1" FT /translation="MKLVFRWFGEKHDTVTLEQIRQIPGVEGVVGALFDIPVGEVWPLE FT EIMKLKETVERAGLKLEVIESVNVHEDIKLGLPTRDRYIENYKETIRNLAKAGVKVVCY FT NFMPVFDWMRTDLHKKLPDGSETMEYDHSLIEGVTPDELIERVKEGSEGFVLPGWEWDR FT LEKLRETFELYKNVDEEKLFENLVYFLERVIPVCEECDVKLAIHPDDPPWSIFGLPRII FT TNKENIERLLKAVDSPYNGITLCMGSLGANPENNIPEMIRYFGKMGRIHFAHVRNLKFT FT GEKSFYETAHPSFCGSHDLFEVMKAFHDIGYEGYIRPDHGRLIWGEKARPGYGLYDRAL FT GATYILGLWEAISKMKERYC" FT terminator 1293..1313 FT /citation=[1] FT RBS 2557..2562 FT /citation=[1] FT CDS 2571..>3015 FT /transl_table=11 FT /gene="xp148" FT /product="x protein" FT /note="putative" FT /db_xref="GOA:Q56324" FT /db_xref="InterPro:IPR000914" FT /db_xref="UniProtKB/TrEMBL:Q56324" FT /citation=[1] FT /protein_id="CAA90234.1" FT /translation="MRKFLVILFVVSIFTLFFAQQLPPGIPRNETFIADNLTGRAANPG FT NFNIWATWVWNDRGIQNLLLEPLWCVEYATGEIINALAAEPPKYNSDFTELTIKLRKGV FT YWSDGVPFTADDLIYTIELVKNTPGMGYNSQMQQVKEIVKVDDY" FT RBS complement(2362..2368) FT CDS complement(1313..2353) FT /transl_table=11 FT /gene="xynB" FT /product="xylanase" FT /function="1,4-beta-D-xylan-xylanohydrolase" FT /EC_number="3.2.1.8" FT /db_xref="GOA:Q60041" FT /db_xref="InterPro:IPR001000" FT /db_xref="InterPro:IPR013781" FT /db_xref="InterPro:IPR017853" FT /db_xref="UniProtKB/Swiss-Prot:Q60041" FT /citation=[2] FT /citation=[1] FT /protein_id="CAA90235.1" FT /translation="MKGLPALLLLLIGCVSSFGSQDVPLRVLAEKLNIHIGFAAGNNFW FT SLPDAEKYMEVAKREFNILTPGNQMKWDTIHPERNRYNFEPAEKHVEFALKNDMIVHGH FT TLVWHNQLPGWLTGQEWSKEELLNILEDHVKTVVSHFRGRVKIWDVVNEAVSDSGTYRE FT SIWYRTIGPEYIEKALIWAKEADPDAILIYNDYNIEEINAKSNFVYNMIKNLREKGVPI FT DGIGFQMHIDYRGINYESFKKNLERFAELGLQIYITEMDRGFPLGGSVGYYLKKQAEVY FT RRIFEICLDNPAVRAIQFWGFTDKYSWVPGFFKGYGKALIFDENYNPKPCYFAIRELME FT EKLKER" FT terminator complement(1293..1313) XX SQ Sequence 3015 BP; 841 A; 701 C; 714 G; 759 T; 0 other; gatcttacgt ttcgaagata aagttcggtg atcccgactc tgtgaatgat catctgaaac 60 cgatcctttc cagccagcag cttttcagag tgaacctgta cgaagttgga cttggagaaa 120 aggtggaagg actgttcaaa aagatgatca caggacctgg cgcggtgaga aaaacactcg 180 aagaagtcat cgggagggaa gatgaatgaa acttgtcttc agatggttcg gagaaaaaca 240 cgacacagta accctggaac agatacgcca gatcccgggt gtggagggag tggtcggtgc 300 actctttgac atccctgtgg gagaagtctg gccccttgaa gagatcatga aactcaagga 360 aaccgttgaa agggctggcc ttaagctgga ggttatcgag agtgtgaatg tccacgaaga 420 cataaaactg ggacttccaa caagagacag gtatatagag aactacaagg aaacgatcag 480 aaatctggca aaggctggtg taaaggtagt ttgctacaac ttcatgcctg tctttgactg 540 gatgagaacg gatcttcaca aaaaactccc ggacggctct gaaacgatgg aatacgacca 600 cagtctcatc gagggagtca ctccagacga actgatagaa cgtgtgaagg aaggatctga 660 gggattcgtc cttcctggat gggagtggga cagactggaa aaattgagag aaacgtttga 720 actctacaaa aacgttgatg aagaaaagct ttttgaaaac ctcgtttatt tcctcgagag 780 ggtcattccc gtctgtgaag aatgcgacgt gaaactcgcc atacacccgg acgatccacc 840 atggagcatc ttcggccttc caaggatcat caccaacaaa gagaacattg aaagactcct 900 caaagcagtc gacagtccat acaacggtat cacgctgtgc atgggctcac tcggtgcgaa 960 cccggaaaac aacatccctg agatgatcag gtatttcgga aagatgggaa ggattcactt 1020 tgcacacgtg agaaacctga agttcaccgg cgaaaaaagc ttctacgaaa cggcccatcc 1080 atctttctgt ggctcgcacg atctatttga ggtgatgaag gcgtttcatg atataggcta 1140 cgaagggtac atccgtccgg accatggaag actgatctgg ggagaaaaag cacggcctgg 1200 ctacggtctc tacgacagag cactcggtgc cacctacatc ctcggtctct gggaagcgat 1260 tagcaagatg aaagagaggt actgctgaga agaaaggggg catcgccccc ttttatcttt 1320 ccttcagttt ttcctccatg agttccctta ttgcaaaata gcacggtttt gggttgtagt 1380 tttcatcgaa gatcaacgcc ttgccgtaac ctttgaagaa acccggcacc caggagtatt 1440 tgtctgtgaa tccccagaac tggatcgccc tgacagcggg gttgtccaga cagatctcga 1500 agatcctcct gtagacttcc gcctgttttt tcagatagta ccctaccgat ccaccaaggg 1560 gaaacccacg gtccatttcc gtgatgtaga tctgaagccc aagctctgcg aatctttcca 1620 ggtttttctt gaaactctcg tagttgattc ccctgtaatc tatatgcatc tgaaacccta 1680 ttccatctat gggaactcct ttttccctca ggttctttat catgttgtag acgaagttgg 1740 acttggcgtt gatctcttcg atgttgtaat cgttgtagat gagaatcgca tccggatctg 1800 cttcctttgc ccagatgagg gccttttcaa tgtactcagg accgatcgtt ctgtaccata 1860 tgctttctct gtaggttcca gagtcactca ccgcttcgtt cacaacatcc cagatcttca 1920 ctctgcccct gaagtgagat accaccgtct ttacgtgatc ttcaagaatg ttcaggagtt 1980 cttctttcga ccactcctgg cctgtgagcc atccaggaag ctggttgtgc cagacgaggg 2040 tgtgaccgtg cacaatcata tcgtttttca gggcaaattc aacatgtttt tcggcaggtt 2100 caaagttgta tctgtttctt tccggatgga tggtgtccca cttcatctgg tttccgggcg 2160 tgaggatgtt gaactctctt tttgcaactt ccatgtactt ttctgcgtcg ggaagagacc 2220 agaagttgtt ccctgctgca aatccgatgt gaatgttcaa cttctctgcg agcactctca 2280 agggaacatc ctgtgaaccg aaggacgaaa cacacccgat cagaagtaac agcagagcag 2340 gcaacccttt cataacatga aacctccttt ttcatttgtg tacggaaata tgataccata 2400 aagaaaaaag ggaaaaagaa gaaaaacgac aatattctga aataatctcc tgtagtccgg 2460 aataatcttc aatatttatg aaaaatggat cttgattttt cagggagatt tacattaaaa 2520 tatttcccga aaggaaaaaa tatacataca atcaagggag gtgagttttc atgaggaagt 2580 ttcttgtgat cctgttcgtt gtttccatct tcaccctgtt ctttgcacag caacttccac 2640 cagggattcc cagaaatgag acgttcatag ccgacaacct aacgggtaga gctgccaacc 2700 ccggtaactt caacatctgg gctacttggg tctggaacga caggggtatc cagaatctcc 2760 ttctggaacc actctggtgc gttgagtacg ccaccggaga gatcatcaac gctcttgccg 2820 ctgaacctcc aaagtacaac tccgacttca cagaactcac cataaaactc agaaaaggtg 2880 tctactggag tgatggtgta ccgtttacag cagatgacct aatttatacc atcgagctcg 2940 tcaaaaacac accgggaatg gggtacaact cccagatgca gcaggtaaaa gagatcgtga 3000 aggtggacga ttaca 3015 // |
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