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EBI DbfetchID Z49241; SV 1; linear; genomic DNA; STD; PRO; 2500 BP. XX AC Z49241; XX DT 11-MAY-1995 (Rel. 43, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 4) XX DE P.ruminicola xynA gene and xynB gene XX KW endo-beta-1,4-xylanase; exo-beta-(1,4)-xylanase/beta-(1,4)-xylosidase; KW exo-xylanase; xylanase; xynA gene; xynB gene. XX OS Prevotella ruminicola OC Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; OC Prevotella. XX RN [1] RP 1-2500 RX PUBMED; 7487028. RA Gasparic A., Martin J., Daniel A.S., Flint H.J.; RT "A xylan hydrolase gene cluster in Prevotella ruminicola B(1)4: sequence RT relationships, synergistic interactions, and oxygen sensitivity of a novel RT enzyme with exoxylanase and beta-(1,4)-xylosidase activities"; RL Appl. Environ. Microbiol. 61(8):2958-2964(1995). XX RN [2] RP 1-2500 RA Flint H.J.; RT ; RL Submitted (10-MAY-1995) to the EMBL/GenBank/DDBJ databases. RL Flint H. J., Rowett Research Institute, Nutrition Division, Greenburn Road, RL Bucksburn, Aberdeen, United Kingdom, AB2 9SB XX FH Key Location/Qualifiers FH FT source 1..2500 FT /organism="Prevotella ruminicola" FT /strain="B14" FT /mol_type="genomic DNA" FT /clone_lib="subclone in pUC18 derived from lambda EMBL3 FT clone 5" FT /clone="5/4a" FT /germline FT /db_xref="taxon:839" FT CDS 142..1251 FT /transl_table=11 FT /gene="xynA" FT /product="endo-beta-(1,4)-xylanase" FT /function="xylan hydrolysis" FT /note="belongs to family F (10) of glycoside hydrolases" FT /db_xref="GOA:P48789" FT /db_xref="HSSP:1HIZ" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/Swiss-Prot:P48789" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA89207.1" FT /translation="MRKLTQFCLGLMLLPIAAVAQNQPTMKDVLGKYFLVGTALNSHQI FT WTHDPKIVHAITDNFNSVVAENCMKGEIIHPEEDYYDWHDADQLVKFAEQHKMTVHGHC FT LVWHSQAPKWMFTDKEGKEVTREVLIDRMYHHITNVVKRYKGKIKGWDVVNEAILDNGE FT YRQSPYYKIIGPDFIKLAFIFAHQADPDAELYYNDYSMSIPAKRNAVVKLVKELKAAGC FT RIDAVGMQSHNGFNYPNLEDYENSIKAFIAAGVDVQFTELDVNMLPNPKSFGGAEISQN FT YKYNKELNPYVNGLTKAAQKTFDQQYLSFFKIYRKYVDHIKRVTVWGVDDGSSWLNGWP FT VPGRTNYGLLIDRNYKVKPVVKEIIKLYE" FT CDS 1334..2293 FT /transl_table=11 FT /gene="xynB" FT /product="exo-beta-(1,4)-xylanase/beta-(1,4)-xylosidase" FT /function="xylan hydrolysis" FT /note="belongs to a newly identified family of glycoside FT hydrolases that also includes Bacteroides ovatus xsa (67.8% FT identical amino acids) and C.stercorarium xylA (30.7% FT identical amino acids). " FT /db_xref="GOA:P48791" FT /db_xref="InterPro:IPR006710" FT /db_xref="UniProtKB/Swiss-Prot:P48791" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA89208.1" FT /translation="MKAKYVFPSDYMADPAANVFDGKLYIYPSHDYDSGECFDDDGGHF FT QMKDYHVLCIDGDPMEQDAKDCGKQFGIEDIPWVEKQLWDNDCVEKDGKYYLIYSAKDY FT TGVFHLGVAVADKPEGPFVPEADPIRGSYSIDPCVFKDDDGEIYVYFGGIWGGQLQWYK FT DNKMLKAEHLPEGKEDPLPSRVARMTGDVKQFAEAPRAVIIVDETGKPLPADDPHRFFE FT ASWMHKYNGKYYFSYSTGDTHLLCYAVGDNPYGPFTYQGVILEPVVGWTTHHSIVEYKG FT KWYLFHHDCVPSNDTTWLRSLKVAELEYDAEGHIKTVK" XX SQ Sequence 2500 BP; 739 A; 426 C; 557 G; 778 T; 0 other; ctgcagccct acttcttttg gccatgttta ttatttcaaa gtatgaactg acagatgaga 60 agatagacga aatcaatagg gcgatagaaa tgaaaaatca gcaaaactaa cctatatata 120 cataaattaa tagtagaaaa aatgagaaag ctaacacaat tttgccttgg ccttatgttg 180 ctgccaatag cagcagttgc tcaaaatcag cctacgatga aggatgtctt aggtaaatac 240 ttcttggtcg gaacagcact taatagtcat cagatttgga cgcatgatcc caaaatcgtt 300 catgctataa ctgataattt taattcggtt gtcgctgaaa attgtatgaa aggtgagatt 360 attcatccag aagaggatta ttatgattgg catgatgctg accagttggt taaatttgcg 420 gaacagcata agatgacagt tcatggccac tgtttggttt ggcactcaca ggctccaaaa 480 tggatgttta ccgataagga aggtaaagaa gttacccgtg aggtgctcat cgaccgtatg 540 tatcatcaca ttactaatgt cgttaagcga tataaaggta aaatcaaggg ttgggatgtc 600 gttaacgagg ctatccttga taatggtgaa tatcgtcagt ctccttatta taagatcatt 660 ggtcctgatt ttatcaagct tgcatttatt tttgctcatc aggcagatcc tgatgcagaa 720 ttgtattata atgactattc gatgtctatt cctgctaagc gtaatgctgt agtcaaactg 780 gttaaggagt tgaaagctgc aggatgtcgt attgatgctg taggtatgca gagccataac 840 ggttttaact atcctaatct tgaggattat gaaaattcta tcaaggcttt cattgctgca 900 ggtgtagatg ttcagtttac agaactcgat gtcaatatgc tacctaatcc taagagcttt 960 ggtggtgcag agattagcca gaactataag tataataagg aacttaatcc atatgtaaat 1020 gggttgacta aagctgctca gaagactttc gatcagcagt atctgtcatt ctttaagatt 1080 tatcgtaagt atgtagatca tattaagcgt gtaaccgttt ggggtgtgga cgacggaagc 1140 agctggctga atggttggcc tgtgcctggt cgtaccaact atggtctgct tatcgaccgc 1200 aactacaagg taaaacctgt ggttaaagaa attatcaaac tttatgagta aagcatggct 1260 gctctttgaa tagggtgata ttgctatact caaagagcat tccgatttgt taattctaaa 1320 tatttataag caaatgaagg caaaatacgt gtttccaagt gattatatgg cagaccctgc 1380 tgccaacgtg tttgatggta aactctatat ctatcctagt cacgactatg atagtggtga 1440 gtgttttgat gatgatggtg gtcatttcca gatgaaagac tatcatgtac tctgtattga 1500 tggtgatcca atggaacagg atgccaagga ctgtggtaaa cagtttggta tcgaggatat 1560 cccatgggta gagaagcagt tgtgggataa cgactgtgta gagaaggatg gtaaatacta 1620 tcttatctat agtgccaagg attataccgg tgtgtttcat cttggtgtag cagtagccga 1680 taaacctgag ggaccatttg tgccagaagc agatcctatt cgtggttcgt atagtatcga 1740 cccatgcgtt ttcaaggatg atgatggtga aatttatgtg tattttggtg gtatctgggg 1800 tggtcagctc caatggtaca aggataataa gatgctaaag gctgaacact tgcctgaggg 1860 taaggaagat ccacttcctt ctagagttgc tcgtatgacc ggtgatgtta agcagtttgc 1920 tgaggctcca cgtgctgtca ttatcgtaga cgagacaggt aaacctttac cagcagacga 1980 tcctcaccgt ttcttcgagg cttcatggat gcacaaatat aatggaaagt attattttag 2040 ttatagtact ggagatacac atttactctg ctacgctgta ggcgataatc cttatggtcc 2100 atttacttac cagggtgtta ttcttgaacc agttgtaggt tggactactc atcattctat 2160 tgtagaatat aaaggcaagt ggtatctttt ccatcacgac tgtgtacctt ctaatgacac 2220 tacttggtta cgttctttga aggtagctga gttggaatat gacgctgaag gtcatatcaa 2280 gacagtaaaa taaagattca tatttcttcc tgaaagtttt tttgaaacct aaaatataat 2340 aagcaaatct ttaataataa tataaacgca agcgtgcgaa tatgagcgta aaatggggga 2400 ctcttcacgg agccccccat tattttttcc ctgtttattc tttatttctg atgttctcgg 2460 atatagtctc taggactcat accgtacata ctcttaaaca 2500 // |
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