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EBI DbfetchID Z49111; SV 2; linear; genomic DNA; STD; PRO; 8300 BP. XX AC Z49111; M83659; Y11134; XX DT 01-JUL-1995 (Rel. 44, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 8) XX DE Streptomyces clavuligerus arginine biosynthesis cluster (argCJBDRGH genes) XX KW acetylglutamate kinase; acetylornithine aminotransferas; argB gene; KW argC gene; argD gene; argG gene; argH gene; arginine regulator; KW argininosuccinate lyase; argininosuccinate synthetase; argJ gene; KW argR gene; glutamate N-acetyltransferase; KW N-acetyl-gamma-glutamyl-phosphate reductase. XX OS Streptomyces clavuligerus OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; OC Streptomycineae; Streptomycetaceae; Streptomyces. XX RN [1] RC (sites) RX DOI; 10.1016/0378-1119(95)00667-2 RX PUBMED; 8566818. RA Rodriguez-Garcia A., Martin J.F., Liras P.; RT "The argG gene of Streptomyces clavuligerus has low homology to unstable RT argG from other actinomycetes: effect of amplification on clavulanic acid RT biosynthesis"; RL Gene 167(1-2):9-15(1995). XX RN [2] RC Revised by [6] RA Rodriguez-Garcia A.; RT ; RL Submitted (25-APR-1995) to the EMBL/GenBank/DDBJ databases. RL Rodriguez-Garcia A., Universidad de Leon, Facultad de Biologia, Area de RL Microbiologia, Campus de Vegazana, Leon, Spain, 24071 XX RN [3] RX PUBMED; 1339424. RA Ludovice M., Martin J.F., Carrachas P., Liras P.; RT "Characterization of the Streptomyces clavuligerus argC gene encoding RT N-acetylglutamyl-phosphate reductase: expression in Streptomyces lividans RT and effect on clavulanic acid production"; RL J. Bacteriol. 174(14):4606-4613(1992). XX RN [4] RX DOI; 10.1046/j.1365-2958.1997.4511815.x RX PUBMED; 9282734. RA Rodriguez-Garcia A., Ludovice M., Martin J.F., Liras P.; RT "Arginine boxes and the argR gene in Streptomyces clavuligerus: evidence RT for a clear regulation of the arginine pathway"; RL Mol. Microbiol. 25(2):219-228(1997). XX RN [5] RA Rodriguez-Garcia A., de la Fuente A., Perez-Redondo R., Martin J.F., RA Liras P.; RT "Characterization and expression of the arginine biosynthesis gene cluster RT of Streptomyces clavuligerus"; RL Unpublished. XX RN [6] RP 1-8300 RA Rodriguez-Garcia A.; RT ; RL Submitted (22-MAR-2000) to the EMBL/GenBank/DDBJ databases. RL Rodriguez-Garcia A., Area de Microbiologia, Universidad de Leon, Facultad RL de Biologia, Leon, 24071, SPAIN. XX FH Key Location/Qualifiers FH FT source 1..8300 FT /organism="Streptomyces clavuligerus" FT /strain="ATCC 27064" FT /mol_type="genomic DNA" FT /db_xref="taxon:1901" FT misc_feature 43 FT /gene="argC" FT /note="Trasncription start point" FT /citation=[3] FT /experiment="experimental evidence, no additional details FT recorded" FT protein_bind 71..88 FT /gene="argC" FT /function="transcription control" FT /bound_moiety="ArgR repressor" FT /note="first arg-box of the argC operator" FT /citation=[4] FT /experiment="experimental evidence, no additional details FT recorded" FT protein_bind 91..108 FT /gene="argC" FT /function="transcription control" FT /bound_moiety="ArgR repressor" FT /note="second arg-box of the argC operator" FT /citation=[4] FT /experiment="experimental evidence, no additional details FT recorded" FT RBS 114..118 FT /gene="argC" FT /citation=[3] FT CDS 123..1148 FT /transl_table=11 FT /gene="argC" FT /product="N-acetyl-gamma-glutamyl-phosphate reductase" FT /function="arginine biosynthesis" FT /EC_number="1.2.1.38" FT /db_xref="GOA:P54896" FT /db_xref="InterPro:IPR012280" FT /db_xref="UniProtKB/Swiss-Prot:P54896" FT /citation=[3] FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAB82479.1" FT /translation="MTVRVAVAGASGYAGGEVVRLLLGHPAESWVALTAFSQAGQAVGH FT PHLGPVAGRVFEPISAQAPLAGHDVVFLALPPAQSAALAEEAGARVVDRNMGADFRLRD FT AGVWERFYGSPWRGCWPYGLPESPGAREALAGARRIAVPGCYPTAVTLALFPAVGAGLV FT DREVVTAVSGTSGAGRALKPHLLGAEVMGSVSPYAVGGTHRHTPEIAQNSTAVTDGEPV FT SVSFTPLLAPMPRGILATCSARLTPGTDARQVRAVYEKTYADEPFVSLLPEGVWPSTGA FT VLGSNQVQVQVAVDPAADRLVVVSAIDNLTKGTAGGALQSMNLALGLPETTGLPRTGLA FT P" FT repeat_region 193..217 FT /gene="argC" FT /rpt_type=INVERTED FT /rpt_unit_range=193..202 FT /note="unknown function, calculated free energy=-20.8 FT kcal/mol" FT /citation=[5] FT repeat_region 1100..1127 FT /gene="argC" FT /rpt_type=DIRECT FT /rpt_unit_range=1100..1109 FT /citation=[5] FT RBS 1210..1214 FT /gene="argJ" FT CDS 1219..2370 FT /transl_table=11 FT /gene="argJ" FT /product="glutamate N-acetyltransferase" FT /function="arginine biosynthesis" FT /EC_number="2.3.1.35" FT /db_xref="GOA:Q9LCS7" FT /db_xref="InterPro:IPR002813" FT /db_xref="UniProtKB/Swiss-Prot:Q9LCS7" FT /citation=[5] FT /citation=[3] FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAB82480.1" FT /translation="MTVTAPKGSTGGGCRRGSKESGQPDLALVVNEGPRRAAAGVFTAN FT RVKAAPVLWSQQVLRGGEVSAVVLNSGGANACTGPKGFQDTHATAERAAAALGHSAAEI FT AVASTGLIGTYLPMDRLLPGIDKAAAELSPHGGERAAIAIRTTDTVHKTAVRAGEGWSV FT GGMAKGAGMLAPGLATLLVVLTTDADLAPPVLDKAVRDAVRTTFERVDSDGCMSTNDTV FT LLLASGASGGDPGYEEFADVLREVCEDLARKLIGDAEGASKDIRIEVIHAATEDDAVEV FT GRTIARNNLLKCAIHGEDPNWGRVLSAIGTTKAVFEPERLNVAINGVWVCREGSAGDGR FT DLVDMRFREVRITADLDAGTHSAVIWANDLTAEYVHENSAYSS" FT repeat_region 2423..2451 FT /gene="argB" FT /rpt_type=DIRECT FT /rpt_unit_range=2423..2433 FT /citation=[5] FT RBS 2474..2478 FT /gene="argB" FT CDS 2485..3393 FT /transl_table=11 FT /gene="argB" FT /product="acetylglutamate kinase" FT /function="arginine biosynthesis" FT /EC_number="2.7.2.8" FT /db_xref="GOA:Q9LCS6" FT /db_xref="HSSP:1GS5" FT /db_xref="InterPro:IPR001057" FT /db_xref="UniProtKB/Swiss-Prot:Q9LCS6" FT /citation=[5] FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAB82481.1" FT /translation="MTRKNNALPKARTLIEALPWLTRHHGKTIVVKFGGNAMVNEELKG FT AFARPSFLVHHAGLRPVVVHGGGPQISAELDRYGLVSEFKAGLRVTTDEAMDVVRMVLA FT GQVQRDVWGCSIVHGPLAVGITGEDAHPISATRHRPLIDGEPVDIGRVGEVTRIDTGAV FT LALLEDGRIPVVSSIARAEDDGHVYNVNADTAAAALAAALGAETLMVLTDVEGLYEDWP FT NSDEVISRLTASELETLLPDLSSGMVPKMEGCLHAVRNGVRTARVIDGRVPHSILLEIF FT TDDGVGTMVVPDEPSVSEGTS" FT RBS 3379..3382 FT /gene="argD" FT CDS 3390..4592 FT /transl_table=11 FT /gene="argD" FT /product="acetylornithine aminotransferase" FT /function="arginine biosynthesis" FT /EC_number="2.6.1.11" FT /db_xref="GOA:Q9LCS5" FT /db_xref="HSSP:2DKB" FT /db_xref="InterPro:IPR015421" FT /db_xref="UniProtKB/Swiss-Prot:Q9LCS5" FT /citation=[5] FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAB82482.1" FT /translation="MSNQEFAQRWQGVMIDSYGTPGLSFVRGEGSTLWDADGTAYTDFV FT SGLAVNALGHAHPAVVGAVSRQIASLGHISNFYSAEPTITLAERLIELFGRPGRVFFCN FT SGAEANETAFKIGRLTGRSRIVAAQSGFHGRTMGSLALTGQPAKREPFLPLPGDVTHVP FT YGDAEALRAAVTEDTAMVILEPIQGESGVVVPPKGYLRAAREITEATGTLLVLDEVQTG FT IGRTGHWFAAQAEGVEADVVTLAKGLGGGLPLGAAVAFGRAAELMTPGHHASTFGGNPV FT SCARTRVLDTIAADGLLDRVKQLGSAPDEWSVRDAAAIRWSPMSGAGLMLGIVLNEPLA FT PQVQLAAQKAGFLVNVPAPDVVRLIPPLVIEETEVDAFLQALPGLLDAVHERDREGQTG FT E" FT misc_feature 4594 FT /gene="argR" FT /note="Transcription start point" FT /citation=[5] FT /experiment="experimental evidence, no additional details FT recorded" FT CDS 4600..5136 FT /transl_table=11 FT /gene="argR" FT /product="arginine regulator" FT /function="arginine metabolism regulation" FT /db_xref="GOA:P95721" FT /db_xref="HSSP:1B4B" FT /db_xref="InterPro:IPR011991" FT /db_xref="UniProtKB/Swiss-Prot:P95721" FT /citation=[4] FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAB82483.1" FT /translation="MTEAHATDAGGPAVPQTRMARHRRIVDILNRQPVRSQSQLAKLLA FT DNGLSVTQATLSRDLDELGAVKIRNTMARLIYAVPARGVPHSAGALGESAKEERMRRLA FT GELLISAEASANLVVLRTPPGAAQFLASAIDQAELHDILGTIAGDDTLMLISRSPTGGQ FT ALADHLLRLAQNDRA" FT repeat_region 5170..5196 FT /gene="argR" FT /rpt_type=INVERTED FT /rpt_unit_range=5170..5180 FT /note="putative transcriptional terminator, calculated free FT energy=-34.6 kcal/mol" FT /citation=[5] FT /citation=[4] FT repeat_region 5206..5225 FT /gene="argR" FT /rpt_type=INVERTED FT /rpt_unit_range=5206..5213 FT /note="putative transcriptional terminator, calculated free FT energy=-14.6 kcal/mol" FT /citation=[5] FT /citation=[4] FT protein_bind 5294..5311 FT /gene="argG" FT /function="transcriptional control" FT /bound_moiety="ArgR repressor" FT /note="third arg-box of the argG operator" FT /citation=[1] FT /citation=[4] FT repeat_region 5303..5327 FT /gene="argR" FT /rpt_type=INVERTED FT /rpt_unit_range=5303..5312 FT /note="putative transcriptional terminator, calculated free FT energy=-16.2 kcal/mol" FT /citation=[5] FT /citation=[4] FT protein_bind 5346..5363 FT /gene="argG" FT /function="transcriptional control" FT /bound_moiety="ArgR repressor" FT /note="First arg-box of the argG operator" FT /citation=[1] FT /citation=[4] FT -35_signal 5347..5352 FT /gene="argG" FT /citation=[1] FT protein_bind 5366..5383 FT /gene="argG" FT /function="transcriptional control" FT /bound_moiety="ArgR repressor" FT /note="Second arg-box of the argG operator" FT /citation=[1] FT /citation=[4] FT -10_signal 5370..5375 FT /gene="argG" FT /citation=[1] FT misc_feature 5382 FT /gene="argG" FT /note="Transcription start point" FT /citation=[5] FT /experiment="experimental evidence, no additional details FT recorded" FT RBS 5399..5404 FT /gene="argG" FT /citation=[1] FT CDS 5411..6604 FT /transl_table=11 FT /gene="argG" FT /product="argininosuccinate synthetase" FT /function="arginine biosynthesis" FT /EC_number="6.3.4.5" FT /db_xref="GOA:P50986" FT /db_xref="HSSP:1KP2" FT /db_xref="InterPro:IPR014729" FT /db_xref="UniProtKB/Swiss-Prot:P50986" FT /citation=[1] FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA88926.1" FT /translation="MTERVVLAYSGGLDTSVAIGWIAEETGAEVIAVAVDVGQGGEDLD FT VIRKRALACGAVEAEVADAKDEFAEEYCLPAIKANARYMDRYPLVSALSRPAIVKHLVA FT AAKKHDASIVAHGCTGKGNDQVRFEAGIQALGPDLKCIAPVRDYAMTRDKAIAFCEAKQ FT LPIATTKKSPYSIDQNVFGRAIETGFLEDIWNAPIEDIFEYTSNPAEPREADEVVISFA FT SGVPVAIDGRPVTVLQAIQQLNERAGAQGIGRIDMVEDRLVGIKSREVYEAPGAIALIT FT AHQELENVTVERELARFKRQVEQRWGELVYDGLWFSPLKRALDGFIEEANQQVTGDIRM FT TLHAGTAVVTGRRSERSLYDFDLATYDTGDTFDQSKAQGFIDIFALSAKIAAKRDLV" FT repeat_region 6628..6662 FT /gene="argG" FT /rpt_type=INVERTED FT /rpt_unit_range=6628..6640 FT /note="putative transcriptional terminator, calculated free FT energy=-37.2 kcal/mol" FT /citation=[5] FT /citation=[1] FT RBS 6682..6686 FT /gene="argH" FT /citation=[1] FT CDS 6695..8116 FT /transl_table=11 FT /gene="argH" FT /product="argininosuccinate lyase" FT /function="arginine biosynthesis" FT /EC_number="4.3.2.1" FT /db_xref="GOA:P50988" FT /db_xref="HSSP:1AOS" FT /db_xref="InterPro:IPR000362" FT /db_xref="UniProtKB/Swiss-Prot:P50988" FT /citation=[1] FT /citation=[5] FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA88927.2" FT /translation="MSSNNGDVRLWGGRFADGPADALARLSASVHFDWRLAPYDIAGSR FT AHARVLNRAGLLTEDELTRMLAGLDRWPPMWRTLLHRTIADEDVHTALERGLLERLGAE FT LGGKLRAGRSRNDQVATLFRMYLRTTPGSSGLIAELQDALVGLAEAHPEVAMPGRTHLQ FT HAQPVLFAHHVLAHVQALSRDAERLRQWDARTAVSPYGSGALAGSSLGLDPEAVAADLG FT FEGGSAGNSIDGTASRDFVAEFAFITAMAGINLSRLAEEIIIWNTKEFSFVTLHDAFST FT GSSIMPQKKNPDIAELARGKSGRLIGNLTGLLATLKALPLAYNRDLQEDKEPVFDSCDQ FT LEVLLPAFTGMVATLTVHRERMEELAPAGFSLATDIAEWLVRQGVPFRVAHDVAGACVK FT ECESAGIELHQLTDEQFAAISEHLTPEVRSVLTVRGALASRDGRGGTAPSAVAVQLAEV FT KADLAVQHAWAARES" FT repeat_region 8162..8192 FT /gene="argH" FT /rpt_type=INVERTED FT /rpt_unit_range=8162..8172 FT /note="putative transcriptional terminator, calculated free FT energy=-20.2 kcal/mol" FT /citation=[5] FT repeat_region 8171..8190 FT /gene="argH" FT /rpt_type=INVERTED FT /rpt_unit_range=8171..8178 FT /note="putative transcriptional terminator, calculated free FT energy=-12.6 kcal/mol" XX SQ Sequence 8300 BP; 1241 A; 3032 C; 2875 G; 1152 T; 0 other; ctgacgcctc ccgcctcctg ccgccccacc ggtcggcgcg tcgcgcgagg cccggccgtc 60 ccaccacctc attgcataaa agtgcagtga tttgtatagt catgccttca gggaggagag 120 tcatgacggt aagggtggcg gttgcgggtg ccagtggcta tgccggtggc gaggtggtgc 180 gtctgctgtt gggccacccg gcggaaagct gggtggcgct gacggcgttc tcgcaggcgg 240 gccaggcggt gggtcatccg cacctggggc cggtggcggg gcgggtgttc gagccgatct 300 cggcccaggc gcccctggcg gggcacgacg tggtgttcct ggcgctgccg ccagcgcagt 360 cggccgccct ggcggaggag gctggggccc gagtcgtgga tcgcaacatg ggggcggact 420 tccggctgcg ggacgcgggt gtgtgggagc gcttctacgg ctcgccgtgg cgcggatgct 480 ggccctacgg gctgccggaa tccccgggcg cccgggaagc cctggcgggg gcccggcgaa 540 tcgcggtacc gggctgctac ccgaccgccg tgaccctggc cctgttcccc gccgtcggcg 600 ccggactggt ggaccgggag gtcgtcaccg ccgtctcggg cacctccggg gcgggccgcg 660 ccctgaagcc gcatctgctg ggcgcggagg ttatgggctc cgtctccccc tacgccgtcg 720 gcggcaccca ccgccacacc cccgagatcg cccagaactc aaccgccgtc acggacggcg 780 aacccgtctc ggtgtctttc acaccactgc tcgcccccat gccccgcggc atcctcgcca 840 cctgctcggc acgtctcacc ccgggcaccg acgcacggca ggtccgcgcg gtctacgaga 900 agacgtacgc ggacgaaccc ttcgtctccc tgctgccgga gggcgtgtgg ccctcgaccg 960 gggcggtcct cggctccaac caggtgcagg tgcaggtggc ggtggacccg gccgcggacc 1020 ggctggtcgt cgtcagcgcg atcgacaacc tgaccaaggg caccgccgga ggcgccctcc 1080 agagcatgaa cctcgccctc ggcctccccg agaccaccgg cctcccccga accggcctcg 1140 ccccctgacc gccgccccgc gaggaaccac gccctgtccc acccccgcac cacctgaccg 1200 agagagagca ggagctccat gacagtcacg gccccgaagg gttcaaccgg cggcgggtgt 1260 cgccgcggat ccaaggagag cggccagccc gatctggcac tggtggtgaa cgaggggccg 1320 cggcgcgctg cggcgggcgt gttcaccgcc aaccgggtca aggccgcgcc cgtgctgtgg 1380 tcgcagcagg tgctgcgcgg tggcgaggtc tccgcggtgg tcctcaactc cggcggcgcg 1440 aacgcctgca ccggaccgaa gggcttccag gacacccacg ccaccgccga gcgggccgcc 1500 gccgccctcg gccacagcgc cgcggagatc gcggtggcgt cgaccgggct gatcggcacg 1560 tatctcccga tggaccggct gctgcccggg atcgacaagg ccgcggccga gctgtccccg 1620 cacggcggcg agcgggccgc gatagcgatc aggaccacgg acaccgtgca caagacggcc 1680 gtccgcgcgg gcgagggctg gagcgtgggc gggatggcca agggcgcggg catgctcgcc 1740 ccgggcctcg ccaccctgct cgtcgtcctg accaccgacg ccgacctcgc gcccccggtc 1800 ctggacaagg cggtgcggga cgcggtccgt accacgttcg aacgggtcga ctccgacggc 1860 tgtatgtcga ccaacgacac cgtgctgctg ctggcgtccg gtgcctcggg gggtgacccg 1920 gggtacgagg agttcgccga cgtgctgcgc gaggtctgcg aggacctggc gcggaagctg 1980 atcggcgacg cggagggcgc gtccaaggac atccggatcg aggtcatcca cgcggcgacc 2040 gaggacgacg cggtcgaggt gggccggacg atcgcccgga acaacctcct caagtgcgcc 2100 atccacggcg aggaccccaa ctggggccgg gtgctgtccg cgatcggcac caccaaggcc 2160 gtcttcgaac ccgagcggct gaacgtcgcc atcaacgggg tctgggtctg ccgcgagggc 2220 agtgcgggcg acggccggga cctcgtcgac atgcggttcc gggaggtccg catcaccgcc 2280 gacctggacg cgggcaccca ttccgccgtg atctgggcca acgacctgac cgccgagtac 2340 gtgcacgaga acagcgcgta cagctcgtga cctcgacggc ggcccaggcg accgcgaccc 2400 ccgggaccac ggcgaagacc accacgacca ccaccaagag cacgaccacc agaacaagaa 2460 cgagaacgag cgcggcggcc agcaatgacc cgtaagaaca acgccctccc caaggcccgg 2520 accctcatcg aggccctgcc ctggctgacc cggcaccacg gcaagacgat cgtggtcaaa 2580 ttcggcggca acgccatggt gaacgaggag ctgaaggggg ccttcgccag accatcgttc 2640 ttggtccacc acgccgggct ccggccggtc gtggtgcacg gcggcggccc ccagatcagc 2700 gccgagctgg accggtacgg cctggtcagc gagttcaagg cgggtctgag ggtcaccacc 2760 gacgaggcca tggacgtggt ccggatggtg ctcgccggac aggtccagcg cgacgtgtgg 2820 ggctgctcaa tcgtgcacgg accgctcgcc gtcgggataa ccggcgagga cgcgcacccc 2880 atcagcgcca cccggcaccg ccccctcatc gacggcgaac cggtcgacat cggccgggtc 2940 ggggaggtca cccgcatcga cacgggcgcc gtcctggcgc tcctggagga cggccgtatc 3000 ccggtggtct cctccatcgc ccgtgcggag gacgacggcc atgtctacaa cgtcaatgct 3060 gatacggcgg ctgcggcact cgctgcggca ctgggcgcgg agaccctcat ggtcctcacc 3120 gacgtcgagg ggctctacga ggactggccg aacagcgacg aggtgatctc ccggctcacc 3180 gcctccgagc tggagacgct gctgcccgat ctctccagcg gcatggtgcc caagatggag 3240 ggctgcctcc acgccgtacg caacggggtc aggaccgccc gggtcatcga cggccgggta 3300 ccgcactcga tcctgctgga gatcttcacc gatgacgggg tcggcacgat ggtcgtgccc 3360 gacgagccgt ccgtatccga ggggacgtca tgagcaacca ggagttcgcc cagcgctggc 3420 agggcgtgat gatcgacagt tatggcaccc cggggctgtc cttcgtccgc ggcgagggct 3480 ccaccctctg ggacgccgac ggcaccgcct acaccgactt cgtcagcggt ctcgcggtga 3540 acgcgctggg ccacgcccac cccgccgtgg tcggggcggt ctcccggcag atcgcgtcgc 3600 tgggccatat ctccaacttc tactcggccg agccgacgat caccctcgcg gagcggctga 3660 tcgagctgtt cggccgcccc ggccgggtct tcttctgcaa ctcgggcgcc gaggccaacg 3720 agaccgcgtt caagatcggc cgactgacgg gccgttcccg gatcgtggcc gcgcagagcg 3780 gcttccacgg ccggaccatg ggctcgctcg ccctcaccgg ccagcccgcc aagcgggaac 3840 cgttcctgcc gctgccgggc gatgtcaccc atgtgccgta cggggacgcc gaggcgctgc 3900 gggccgccgt caccgaggac acggcgatgg tgatcctgga gccgatccag ggcgagagcg 3960 gtgtggtggt cccgccgaag gggtatctgc gggccgcccg ggagatcacc gaggcgacgg 4020 gcaccctgct cgtcctggac gaggtgcaga ccggaatcgg acggaccggc cactggttcg 4080 ccgcccaggc cgagggtgtg gaggcggatg tcgtcaccct cgccaagggg ctcggcggcg 4140 ggctgccgct gggcgcggcg gtcgccttcg ggcgcgccgc cgagctgatg acccccggcc 4200 accacgcctc caccttcggc ggcaacccgg tctcctgcgc gcggactcgc gtcctggaca 4260 ccatcgccgc cgatggactc ctcgaccggg tcaagcagct cgggagcgct ccggacgagt 4320 ggagcgtgcg ggacgcggcg gccatccgct ggtctcccat gtccggtgcg ggactgatgc 4380 tgggtatcgt gctcaacgag ccccttgcgc cccaggtgca gctcgcggcc cagaaggccg 4440 gcttcctggt gaacgtcccg gcccccgatg tcgtacggct gattccgccg ctggtcatcg 4500 aggagaccga ggtggacgct ttcctccagg ccctgcccgg ccttctcgac gcggtccacg 4560 agcgggacag ggaaggacag accggagaat gagacgacga tgaccgaggc gcacgccacc 4620 gacgccggcg ggcctgcggt accgcagacc cgcatggccc gccaccgccg gatcgtggac 4680 atcctcaacc gccagccggt gcgctcgcag agccagctcg ccaagctcct cgcggacaac 4740 ggactgagcg tcacccaggc gacgctctcc cgggacctcg acgagctggg cgcggtgaag 4800 atccgcaaca caatggcgag gctgatctac gcggtgccag cgaggggcgt tccgcactcc 4860 gcaggcgccc tcggcgagtc ggcgaaggag gagcggatgc gcaggctcgc gggggagctg 4920 ctgatctccg cggaggcgtc cgccaatctg gtggtgctgc gtacgccccc cggcgcggcg 4980 cagttcctcg cctcggccat cgaccaggcg gagctgcacg acatcctcgg caccatcgcc 5040 ggggacgaca ccctgatgct gatcagcagg tcgcccacgg gcggtcaggc gctcgccgac 5100 catctgctgc ggctggccca gaacgaccgg gcctgaccgg ccctccggcg cggcgccgcc 5160 acccccgcag gcccccggcc ctctcggccg ggggctgcgg catgccgcgg cgggccgccg 5220 ccgtgcggaa gacccctgcc gctgtccgac ggccccgggg attcctccgt cgacgcgttc 5280 agcgcgccgc tccatggcgt aagcgtccga tcacttcggt cggacgcacc gatcgcgcac 5340 ccatgattga cgaaacatgc gggcgagtgc atacttattc catcaccgca tgcatcgtaa 5400 ggagattcct gtgaccgagc gcgtcgtact cgcctactcg ggcggtctgg acacctccgt 5460 cgccatcggc tggatcgccg aggagacggg cgccgaggtc atcgccgtcg ccgtggacgt 5520 cggccagggc ggcgaggacc tggacgtcat ccgcaagcgc gcgctcgcct gcggtgccgt 5580 ggaggccgag gtggccgacg ccaaggacga gttcgccgag gagtactgcc tccctgcgat 5640 caaggccaac gcccgctaca tggaccggta cccgctggtg tccgcgctct cccggcccgc 5700 gatcgtcaag cacctggtcg ccgccgcgaa gaagcacgac gcctcgatcg tggcgcacgg 5760 ctgcaccggc aaggggaatg accaggtccg tttcgaggcc ggtatccagg ccctcggccc 5820 ggacctcaag tgcatcgcgc cggtccgtga ctacgccatg acccgggaca aggccatcgc 5880 cttctgcgag gccaagcagc tcccgatcgc caccaccaag aagtccccgt actccatcga 5940 ccagaacgtc ttcggccggg ccatcgagac cggcttcctg gaggacatct ggaacgcgcc 6000 gatcgaggac atcttcgagt acacctccaa cccggccgag ccccgcgagg ccgacgaggt 6060 ggtcatctcc ttcgcctccg gtgtcccggt cgccatcgac ggccgccccg tcaccgtgct 6120 ccaggccatc cagcagctca acgagcgcgc gggcgcccag ggcatcggcc ggatcgacat 6180 ggtcgaggac cggctggtcg gcatcaagtc ccgtgaggtg tacgaggcgc cgggcgcgat 6240 cgcgctgatc accgcccacc aggagctgga gaacgtcacc gtcgagcgcg aactggcccg 6300 cttcaagcgg caggtcgagc agcgctgggg cgagctggtc tacgacggcc tgtggttctc 6360 cccgctcaag cgcgccctgg acggcttcat cgaggaggcc aaccagcagg tcaccggcga 6420 catccggatg accctgcacg cgggcaccgc cgtggtcacc ggccgccgct ccgagcggtc 6480 gctgtacgac ttcgacctcg cgacctacga cacgggcgac accttcgacc agtccaaggc 6540 ccagggcttc atcgacatct tcgccctctc cgcgaagatc gccgccaagc gcgacctggt 6600 ctgaccctcc cgtacggccc tcgcgccgcc gcctccccgc cacccccgtg cggggaggcg 6660 gtcccgtacc tccacccttc gaggagttcc cgcagtgagc agcaacaacg gtgatgtccg 6720 gctctggggc ggacggttcg ccgatggacc cgccgacgcg ctggcgaggc tctccgcctc 6780 ggtgcacttc gactggcggc tggcgccgta cgacatcgcg ggctcccgcg cccacgcccg 6840 ggtcctcaac cgggccgggc tgctcaccga ggacgagctg acccggatgc tcgccggact 6900 ggaccgctgg ccgccgatgt ggcggacgct ccttcaccgc accatcgccg acgaggacgt 6960 ccacaccgcc ctggagcggg ggctgctgga gcgcctgggc gcggagctgg gcggcaagct 7020 gcgcgcggga cgctcccgca acgaccaggt ggcgaccctc ttccggatgt atctgcggac 7080 cacgcccgga tcgtcggggc tgatcgccga actccaggac gcgctggtgg gcctggccga 7140 ggcccatccg gaggtggcga tgccggggcg tacccacctc cagcacgccc agcccgtgct 7200 cttcgcccac catgtgctcg cccatgtcca ggcgctgtcc cgggacgccg agcggctgcg 7260 ccagtgggac gcgcggacgg cggtctcgcc ctatggctcc ggggcgctcg cgggctcctc 7320 gctcggcctg gacccggagg cggtcgccgc cgacctgggc ttcgagggcg gttcggcggg 7380 caactccatc gacggcaccg cctcccggga cttcgtcgcg gagttcgcct tcatcaccgc 7440 catggccggg atcaacctct cccggctcgc ggaggagatc atcatctgga acacgaagga 7500 gttctccttc gtcaccctgc acgacgcctt ctccaccggc tcgtcgatca tgccgcagaa 7560 gaagaacccc gacatcgccg agctggcgcg cggtaagtcc ggccggctga tcggcaatct 7620 gaccgggctg ctggcgacgt tgaaggcgct gccgctcgcg tacaaccgcg acctccagga 7680 ggacaaggag ccggtcttcg actcctgcga ccagctggag gtgctgctgc ccgcgttcac 7740 cgggatggtg gccacgctca ccgtgcaccg ggagcggatg gaggagctgg ctccggcggg 7800 cttctcgctc gccaccgaca tcgcggagtg gctcgtgcgg cagggcgtgc cgttccgggt 7860 cgcccacgat gtcgccgggg cgtgcgtcaa ggagtgcgag agcgcgggca tcgaactgca 7920 ccagctcacg gacgagcagt tcgcggcgat ctcggagcat ctgacccccg aggtgcgctc 7980 ggtcctcacc gtccggggcg cgctggcctc ccgcgacggc cggggcggca cggccccctc 8040 ggcggtggcc gtccagctcg ccgaggtcaa ggccgatctc gcggtccagc acgcctgggc 8100 ggcccgcgag agctgacccc gtcccacggc aggggcgcga gcgagtgcca ccgtcccgcg 8160 tacggcggcg gacggtggcg ctccgccgtc gttcccgccg tctccggcac cgggctgcgg 8220 tcatatttaa tgagacgtcg ctgtctcaaa tgcagtatct ttgtctcatg acaattgatc 8280 gtgagcgggt gctccgtgcc 8300 // |
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