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EBI DbfetchID Z47974; SV 1; linear; genomic DNA; STD; PRO; 3786 BP. XX AC Z47974; XX DT 31-DEC-1995 (Rel. 46, Created) DT 15-FEB-2006 (Rel. 86, Last updated, Version 17) XX DE T.neapolitana lamA gene and bglB gene XX KW beta-glucosidase; bglB gene; LAMA gene; laminarinase. XX OS Thermotoga neapolitana OC Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga. XX RN [2] RC (sites) RX DOI; 10.1006/bbrc.1993.1974 RX PUBMED; 8352795. RA Dakhova O., Kurepina N., Zverlov V., Svetlichnyi V., Velikodvorskaya G.; RT "Cloning and expression in Escherichia coli of Thermotoga neapolitana genes RT coding for enzymes of carbohydrate substrate degradation"; RL Biochem. Biophys. Res. Commun. 194(3):1359-1364(1993). XX RN [3] RP 1-3786 RA Zverlov V.V.; RT ; RL Submitted (24-JAN-1995) to the EMBL/GenBank/DDBJ databases. RL Zverlov V. V., Institute of molecular genetics, Russian Academy of RL Sciences, Kurchatov Sq. 46, Moscow, Russia, 123182 XX RN [4] RX PUBMED; 9168619. RA Zverlov V.V., Volkov I.Y., Velikodvorskaya T.V., Schwarz W.H.; RT "Highly thermostable endo-1,3-beta-glucanase (laminarinase) LamA from RT Thermotoga neapolitana: nucleotide sequence of the gene and RT characterization of the recombinant gene product"; RL Microbiology (Reading, Engl.) 143:1701-1708(1997). XX RN [5] RA Zverlov V.V., Volkov I.Y., Velikodvorskaya T.V., Schwarz W.H.; RT "Thermotoga neapolitana bglB gene upstream of lamA, encodes a highly RT thermostable b -glucosidase that is a laminaribiase"; RL Microbiology 143:3537-3537(1997). XX FH Key Location/Qualifiers FH FT source 1..3786 FT /organism="Thermotoga neapolitana" FT /strain="Z2706-MC24" FT /mol_type="genomic DNA" FT /db_xref="taxon:2337" FT RBS 905..910 FT CDS <1..901 FT /pseudo FT /codon_start=2 FT /transl_table=11 FT /gene="bglB" FT /product="beta-glucosidase" FT /EC_number="3.2.1.21" FT /db_xref="PSEUDO:CAA88009.1" FT CDS 918..2858 FT /transl_table=11 FT /gene="lamA" FT /standard_name="1,3-beta-D-glucan-glucanhydrolase" FT /product="laminarinase" FT /EC_number="3.2.1.39" FT /db_xref="GOA:Q60039" FT /db_xref="HSSP:1GUI" FT /db_xref="InterPro:IPR013320" FT /db_xref="UniProtKB/TrEMBL:Q60039" FT /citation=[2] FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA88008.1" FT /translation="MKKLVLVLLLFPVFILAQNILHNGSFDAPILIAGVDIEPPAADGS FT INTQNNWVFFTNSNGEGEARVENGVLVVEITNGGDHTWSVQIIQSPIRVEKLHKYRVFF FT KAKASVQRNIGVKIGGTAGRGWAAYNPGTDESGGMVFELGTDWKTYEFEFVMRQETDEN FT ARFEFQLGKSTGTVWIDTVWIDDVVMEDVGTLEVSGEENEIYTEEDEDKVEDWQLVWSQ FT EFDDGVIDPNVWNFEIGNGHAKGIPGWGNAELEYYTDKNAFVENGCLVIEARKEQVSDE FT YGTYDYTSARITTEGKFEIKYGKIEIRAKLPKGKGIWPALWMLGNNIGEVGWPTCGEID FT IMEMLGHDTRTVLRTAHGPGYSGGASIGVAYHLPEEVPDFSEDFHVFSIEWDENEVEWY FT VDGQLYHVLSKDELAELGLEWVFDHPFFLILNVAMGGYWPGYPDETTQFPQRMYIDYIR FT VYKDMNPETITGEVDDCEYEQSQQQTGPEVTYEQINNGTFDEPIVNDQANNPDEWFIWQ FT AGDYGISGARVSDYGVTDGYAYITIEDSGTDTWHIQFNQWIGLYKGKTYTISFRAKADT FT PRPINVKILQNHDPWINYFAQTVNLTTEWQTFTFTYTHPDDADEVVQISFELGKEAPTT FT IYFDDVSVSPQ" FT terminator 2864..2883 FT /gene="lamA" XX SQ Sequence 3786 BP; 1131 A; 783 C; 1023 G; 849 T; 0 other; gatcataaaa ccaaaacttc cagaaaactt cctttcggag aaggaaatac acaaactggc 60 gaaaaagaac gacgtggcgg tcatcgtgat cagcaggatt tccggagaag gctatgacag 120 aaagccggtg aagggagact tttacctttc tgacgatgag actgatctca taaagactgt 180 ctccagagag ttccatgaac agggcaagaa agtgatcgtt cttctcaaca taggaagtcc 240 tgttgaggtt gttagctgga gagatctggt ggatgggatt ctccttgtgt ggcaggcagg 300 gcaggaaacc ggcaggatcg ttgccgatgt tctcactgga aggatcaatc catctggaaa 360 acttccaacc acctttccga gagactactc tgatgtaccc tcctggacct ttcctggaga 420 gccaaaggac aatccacaga aggtggtcta cgaagaggac atctacgtgg gatacaggta 480 ctacgacacc ttcggtgtgg agccggcgta cgagttcgga tacggccttt cttacacgac 540 ctttgagtac agtgacctga acgtttcgtt cgacggtgaa acactcagag ttcagtacag 600 aatagaaaac acgggcggtc gtgcaggaaa ggaagtctcg caggtttaca tcaaggcacc 660 gaaaggaaaa atcgacaaac ccttccagga actcaaggcg tttcacaaaa ccagactttt 720 gaatcctgga gagtctgaag aagtggtgct tgagatacct gtcagagatc ttgcaagttt 780 caacggtgaa gaatgggttg tcgaagcggg tgaatacgaa gtaagggttg gtgcgtcttc 840 gaggaacata aaacttaaag gaacgttttc cgtcggtgag gagagaagat tcaaaccgtg 900 aaggaggagg gggtatcatg aaaaaactgg ttcttgttct tttgctattt cctgttttca 960 tcctggctca gaatatttta cacaatggct ccttcgacgc tcccattctc atcgctggtg 1020 tcgatatcga accacctgca gcagacggtt ctataaacac gcagaacaac tgggtgtttt 1080 tcaccaattc gaacggtgag ggcgaagcaa gggtggaaaa tggcgttctc gtggttgaaa 1140 taacaaacgg aggagaccac acctggtctg ttcagataat ccagtccccc atacgtgttg 1200 agaaactcca caagtacaga gtttttttca aggcaaaggc ctccgttcag agaaacatag 1260 gagtgaaaat aggaggaacg gcaggaaggg gctgggcagc ttacaatccc ggaacggatg 1320 agtcgggagg tatggttttt gaacttggaa cagactggaa gacgtacgag ttcgaatttg 1380 tcatgagaca ggaaacggat gaaaacgcac gctttgagtt ccagcttgga aagtcaacgg 1440 ggacggtctg gatagacacg gtctggatag acgacgtagt gatggaagat gtgggaacgc 1500 tggaagtcag tggtgaggaa aacgagatct acaccgagga ggatgaggac aaggtggaag 1560 actggcaact tgtctggagt caggagttcg atgatggagt tatcgatcca aacgtatgga 1620 actttgagat aggaaacggc catgcaaagg gcatacctgg ctggggaaac gcagaactcg 1680 agtattacac cgacaaaaac gcattcgttg aaaacggctg cctcgtgata gaagcaagga 1740 aagaacaggt atccgatgag tacggaacct acgactacac atccgcaagg ataaccactg 1800 aaggtaaatt cgaaataaag tacggaaaga tcgagataag ggcgaagctt cccaaaggaa 1860 agggcatctg gccggctctc tggatgcttg gaaacaacat cggtgaagtt ggatggccca 1920 cctgtggtga gatcgacatc atggagatgc tggggcacga cacaagaacc gttttacgaa 1980 cagcgcacgg tccagggtat tctggcggtg caagtatagg tgtggcttat catcttcccg 2040 aagaggttcc tgacttctca gaagacttcc acgtcttctc catcgagtgg gacgaaaacg 2100 aagtggaatg gtatgtagat ggccagctgt atcacgttct cagcaaggac gaacttgccg 2160 aacttggact cgaatgggtc ttcgatcacc cgttcttcct catactgaac gttgcgatgg 2220 gaggttactg gccgggctat cctgacgaaa caacgcagtt tccacagaga atgtacatcg 2280 attacatcag agtttacaaa gatatgaatc cagaaactat cacaggagaa gtggacgatt 2340 gtgagtacga acagtctcag cagcaaacag ggcccgaagt gacctatgaa cagataaaca 2400 acggcacatt cgatgaaccg atagtgaacg atcaggccaa caacccggat gagtggttca 2460 tatggcaggc cggtgactat ggaatcagcg gtgccagagt ttctgactac ggtgtcacag 2520 atggctacgc gtacatcacg atagaggact ccggaacaga tacctggcac atacagttca 2580 accagtggat aggcctctac aaaggaaaga cctacaccat ttctttcagg gctaaagcag 2640 atacaccaag accgataaac gtgaagatcc ttcagaatca cgatccatgg atcaactact 2700 ttgcgcagac agtcaatctc acaacagaat ggcagacgtt tacgttcacc tacacacatc 2760 cagacgatgc ggacgaggtt gttcagatca gtttcgaact tggaaaagag gctccaacca 2820 cgatttactt cgacgatgtt tcggtgagcc ctcaatgatt tctccccggc ggtaatgccg 2880 gggttttcaa actgaaccga agaggggaga tcgagagtga acataagagc aaggattgtc 2940 ttcataatga tcgtagttct tttgtctttt gtgttgagtg ctcttttgat ccagaggaac 3000 acctcgaatc ttcttgtaaa cagtgcaaag gagtacatgg agaagtccgt ggtgtctctt 3060 tcaaagtatg tttctcaaag gttgaatgag gttcagaaga atttgaaaac ccttcttgga 3120 agttccctca taggtggtta tacgatcgcg tcgaaccttc aaaccgttct tcagggggct 3180 actgatacag cttctttggg tctggtgatc gatgaaatga gtgaatctgc ctatctcgtc 3240 ttgcccgaga agatagaaga gaagaactat tcagaatacg agagatatct gaaacttctg 3300 aaagagagca accgaacatc gcttgttctg gcagagaaaa tcggggaaag tccgatgctg 3360 ctgttcatgg agggtatcac cacttttgga acggagccat cgggagcact cgctcttgga 3420 gtatcccttt ctgaaaattc aaatctctgg aaaaccgtag tagaagaggg gaaagcgagc 3480 aaaagcggct acggcatact ggtatccggt gatgggaaag tattgataca caaagacatg 3540 aaaaacttca tgaaagacgt gaaagaactt ggtggatttg agaaagcttt cagtgaagca 3600 aagagtggag gagaaaggta cgtggagtac gaagaaaacg gagaaaagaa atacaccgta 3660 tgggcgaaag tgccaggata cgacttttac atcttctcga caggatacct caacgaactt 3720 ctttcagaag gaaggaaagc gacctttgga acgatagtga cgtacgtggt atttggaggc 3780 gtgatc 3786 // |
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