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EBI DbfetchID Z36941; SV 1; linear; genomic DNA; STD; PRO; 2582 BP. XX AC Z36941; XX DT 06-SEP-1994 (Rel. 40, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 2) XX DE B.subtilis defective prophage PBSX xhlA, xhlB, and xylA genes XX KW holin; lysis gene; N-acetylmuramoyl-L-alanine amidase; xhlA gene; KW xhlB gene; xyla gene. XX OS Bacillus subtilis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-2582 RA Krogh S., Joergensen S.T., Diderichsen B., Devine K.M.; RT "Sequence of the lysis gene cluster of PBSX"; RL Unpublished. XX RN [2] RP 1-2582 RA Krogh S.; RT ; RL Submitted (03-SEP-1994) to the EMBL/GenBank/DDBJ databases. RL Susanne Krogh, Genetics, Trinity College, Lincoln Place Gate, Dublin 2, RL Ireland XX DR InterPro; IPR010022; Phage_XkdX. DR UniProtKB/Swiss-Prot; P54343; XKDX_BACSU. XX FH Key Location/Qualifiers FH FT source 1..2582 FT /organism="Bacillus subtilis" FT /strain="Bacillus subtilis SO113" FT /mol_type="genomic DNA" FT /proviral FT /db_xref="taxon:1423" FT RBS 113..119 FT CDS 126..965 FT /transl_table=11 FT /gene="ORF1" FT /product="homlogues of ORF1 are associated with cell lysis FT genes" FT /db_xref="UniProtKB/Swiss-Prot:P39797" FT /protein_id="CAA85400.1" FT /translation="MVKYQYEFPLDKAGKAGAVKPYRGGKNDFVTPVSNLSGVAEILTN FT AALKATEAYSQLGQDRLGAVLISKVKGWAYADREGTLFIEESDNNNVWTTTAAVNVAAG FT VLTATDWVYLSKRYYRFRYVNGNLQQSEFVLYQSVGAGEMDVRVNEKTPLQIDFAENQT FT HDGRLKVEARKTFDFVFHENAESASEGAALPVDGAAHLLVEVYGTAEMSEVKFWGKSVS FT GQKLPIRGVKTDDATTASSTLGKAEAWAFDIKGFKEIIMEIISITGGTLSVKGTAVS" FT RBS 1003..1009 FT CDS 1018..1287 FT /transl_table=11 FT /gene="xhlA" FT /product="protein associated with cell lysis upon induction FT of PBSX" FT /db_xref="GOA:P39798" FT /db_xref="InterPro:IPR019715" FT /db_xref="UniProtKB/Swiss-Prot:P39798" FT /protein_id="CAA85401.1" FT /translation="MQQEADVNVFQQDLADMKGEHKALEQRVSALERVSDRQDQQIMTL FT NEKLNKIEENTTWIKRTITGAIITAVSTGIIGGAIAIMYSLLQH" FT RBS 1287..1293 FT CDS 1300..1563 FT /transl_table=11 FT /gene="xhlB" FT /product="holin" FT /note="gene product involved in cell lysis upon induction FT of PBSX" FT /db_xref="GOA:Q99163" FT /db_xref="InterPro:IPR006479" FT /db_xref="UniProtKB/Swiss-Prot:Q99163" FT /protein_id="CAA85402.1" FT /translation="MNTFDKGTVIRTVLLLIALINQTMLMLGKSPLDIQEEQVNQLADA FT LYSAGSIAFTIGTTLAAWFKNNYVTEKGKKQRDLLRDNNLTK" FT RBS 1562..1568 FT CDS 1576..2469 FT /transl_table=11 FT /gene="xylA" FT /product="N-acetylmuramoyl-L-alanine amidase" FT /db_xref="GOA:P39800" FT /db_xref="HSSP:1LBU" FT /db_xref="InterPro:IPR002482" FT /db_xref="UniProtKB/Swiss-Prot:P39800" FT /protein_id="CAA85403.1" FT /translation="MVNIIQDFIPVGANNRPGYAMTPLYITVHNTANTAVGADAAAHAR FT YLKNPDTTTSWHFTVDDTEIYQHLPLNENGWHAGDGNGSGNRASIGIEICENADGDFAK FT ATANAQWLIKTLMAEHNISLANVVPHKYWSGKECPRKLLDTWDSFKAGIGGGGSQTYVV FT KQGDTLTSIARAFGVTVAQLQEWNNIEDPNLIRVGQVLIVSAPSAAEKPELYPLPDGII FT QLTTPYTSGEHVFQVQRALAALYFYPDKGAVNNGIDGVYGPKTADAVARFQSVNGLTAD FT GIYGPATKEKIAAQLS" FT stem_loop 2483..2507 FT /note="possible transcription terminator of the late operon FT of PBSX" XX SQ Sequence 2582 BP; 762 A; 548 C; 656 G; 616 T; 0 other; aattcaggat ctggcagagg gtgtgaacaa aaagctgatc acagctgacc agtataaaga 60 aattaccggt aaagccatgt aaggcttttt tattttgcct gtttttagat caaaggagga 120 tgaagatggt gaagtatcaa tatgaatttc ctctcgataa ggctggaaaa gccggcgctg 180 taaagcccta tcgaggagga aaaaatgatt ttgtgacacc tgtttcgaat ttgtcaggcg 240 tagcggagat tttaacaaat gctgctttaa aggcgactga ggcatacagt cagctcgggc 300 aggacaggct tggcgcagtt ctgatttcga aagtgaaggg ctgggcgtat gcagatcgtg 360 aaggtacgct ctttatagaa gaaagcgaca acaacaatgt ttggacaacg acagcagcag 420 ttaatgtcgc agcaggcgtc ctgacagcga cggactgggt atatctttct aaacgctatt 480 accgtttccg ctatgtgaac gggaatcttc agcaatctga gtttgtatta taccaatcag 540 tcggtgcggg tgagatggat gtgcgtgtca atgaaaaaac gcctttacag attgactttg 600 cggagaatca aacacacgat ggacggctga aagtcgaggc tcgcaaaaca tttgactttg 660 tctttcatga aaatgcagag tccgccagcg agggggctgc tttacctgtt gacggtgccg 720 cgcatttact cgttgaagtc tacggcacag cagaaatgag cgaagtcaaa ttttggggca 780 aatcggtgtc aggacagaaa ctgccgatca gaggcgtgaa aactgatgat gctaccactg 840 cctccagtac attaggaaaa gctgaggcat gggcctttga tattaaaggg tttaaggaga 900 tcatcatgga gattatcagt atcaccggcg gtactctttc ggtaaaaggg accgcggttt 960 cataatagtc tcggccctcg gaagggaggt gatctgcatg tgaaggagga gtgagagatg 1020 cagcaagagg cagacgtgaa tgtgtttcag caggatttag cagacatgaa aggcgagcac 1080 aaagcacttg agcagagggt ttccgcatta gaacgcgtgt ctgaccggca agaccagcaa 1140 atcatgacgc tgaacgaaaa attaaacaaa attgaagaaa acaccacgtg gattaaacgc 1200 accatcacag gtgccatcat tacagcagtg tctacaggca tcattggcgg agccatcgcc 1260 attatgtaca gcctgctgca gcattaaagg gggattttta tgaacacgtt tgacaagggc 1320 acggtcatca ggacggtgct tcttttaatt gctttaatca accagaccat gctgatgctc 1380 ggcaaatcac cattggacat tcaggaggag caggtcaatc agctcgctga cgctctttat 1440 tcagccggtt ccatcgcatt tacaattgga acgacacttg ccgcttggtt taaaaacaac 1500 tatgtaacag aaaaagggaa aaaacagcgc gacttgttaa gggacaataa tctgacgaaa 1560 taaggagaga tgaaaatggt taacattatt caagacttta ttccggttgg cgcaaataac 1620 cgtccaggct acgcaatgac gccgctttac attacggtgc acaacacagc gaataccgca 1680 gtaggagcag atgctgcagc gcatgcccgc tatttgaaaa atcctgatac gacgacaagc 1740 tggcatttta cagttgatga tacagaaatt tatcagcatc tgcctttaaa tgaaaacggc 1800 tggcatgcgg gagacggaaa tggcagcggc aaccgggctt ctattggaat tgaaatttgc 1860 gaaaatgccg atggcgattt tgcaaaagca acagcaaatg cccagtggct catcaaaaca 1920 ttaatggctg aacataatat cagtctcgcc aatgtcgtcc ctcataagta ttggtcaggg 1980 aaggaatgtc cgcgaaaatt gttagatacg tgggattcgt tcaaagcagg aattggggga 2040 ggcggcagcc aaacttacgt cgtgaaacag ggcgacacac ttacatctat agcgagagca 2100 tttggtgtta cggttgctca gctgcaagag tggaacaata tcgaagaccc gaatcttatt 2160 cgcgttggtc aagtattaat tgtaagtgct ccatcggctg ctgaaaaacc cgagctctat 2220 ccgctccctg atggtatcat tcaattgaca acaccttata catcaggaga acacgtcttt 2280 caggtgcaac gagcacttgc ggctctgtat ttttaccctg ataaaggcgc tgttaacaac 2340 ggaattgacg gcgtttacgg accgaaaaca gctgacgcag ttgcccgttt tcagtctgtt 2400 aacggtctaa ctgctgacgg tatctacggg cctgcgacga aagagaagat cgcagcgcaa 2460 ttaagctgat caaagaccat aaaaatcccg gagccgctcc gggatttatt ttttcttctt 2520 caactttttt ataattccag cactgcgctc cctcacccga agcgagtacc cggaaagccc 2580 aa 2582 // |
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