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EBI DbfetchID Z33876; SV 1; linear; genomic DNA; STD; PRO; 2539 BP. XX AC Z33876; XX DT 23-MAY-1994 (Rel. 39, Created) DT 02-APR-1997 (Rel. 51, Last updated, Version 9) XX DE Bacillus sp. celB1 gene for endo-beta-1,4-glucanase XX KW endo-beta-1,4-glucanase. XX OS Bacillus sp. OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-2539 RA Sanchez Torres J.; RT ; RL Thesis (1994), Microbiologia y Genetica, Universidad de Salamanca XX RN [2] RP 1-2539 RA Sanchez Torres J.; RT ; RL Submitted (20-MAY-1994) to the EMBL/GenBank/DDBJ databases. RL Juan Sanchez Torres, Microbiologia y Genetica, Universidad de, Salamanca, RL Facultad de Biologia, Edif. Interdepartamental.Avda del Campo Charro s/n, RL Salamanca, Salamanca, 37008, Spain XX RN [3] RP 1-2539 RX DOI; 10.1007/s002530050797 RX PUBMED; 8987645. RA Sanchez-Torres J., Perez P., Santamaria R.I.; RT "A cellulase gene from a new alkalophilic Bacillus sp. (strain N186-1). Its RT cloning, nucleotide sequence and expression in Escherichia coli"; RL Appl. Microbiol. Biotechnol. 46(2):149-155(1996). XX FH Key Location/Qualifiers FH FT source 1..2539 FT /organism="Bacillus sp." FT /strain="186-1" FT /isolate="186-1" FT /mol_type="genomic DNA" FT /haplotype="Haploid" FT /clone="pJS3, pJS4 and pJS5" FT /db_xref="taxon:1409" FT -35_signal 821..826 FT /note="homologous to sigmaA-type promoter" FT -10_signal 844..849 FT /note="homologous to sigmaA-type promoter" FT RBS 959..964 FT /note="homologous to 16S rRNA 3'end" FT CDS 970..2139 FT /transl_table=11 FT /standard_name="alkaline cellulase" FT /product="endo-beta-1,4-glucanase" FT /EC_number="3.2.1.4" FT /note="presursor protein" FT /db_xref="GOA:Q59232" FT /db_xref="InterPro:IPR013781" FT /db_xref="PDB:1LF1" FT /db_xref="UniProtKB/TrEMBL:Q59232" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA83942.1" FT /translation="MKKITTIFVVLLMTVALFSIGNTTAADNDSVVEEHGQLSISNGEL FT VNERGEQVQLKGMSSHGLQWYGQFVNYESMKWLRDDWGITVFRAAMYTSSGGYIDDPSV FT KEKVKEAVEAAIDLDIYVIIDWHILSDNDPNIYKEEAKDFFDEMSELYGDYPNVIYEIA FT NEPNGSDVTWDNRIKPYAEEVIPVIRNNDPNNIIIVGTGTWSQDVHHAADNQLADPNVM FT YAFHFYAGTHGQNLRDQVDYALDQGAAIFVSEWGTSAATGDGGVFLDEAQEWIDFMDER FT NLSWANWSLTHKDESSAALMPGANPTGGWTEAELSPSGTFVREKIRESASIPPSDPTPP FT SDPGEYPAWDPTQIYTNEIVYHNGQLWQAKWWTQNQEPGDPYGPWEPLN" FT sig_peptide 970..1044 FT mat_peptide 1045..2136 FT /standard_name="alkaline cellulase" FT /product="endo-1,4-beta-glucanase" FT /EC_number="3.2.1.4" FT stem_loop 2204..2216 FT /note="transcription termination sequence" XX SQ Sequence 2539 BP; 876 A; 379 C; 528 G; 756 T; 0 other; aagcttgaat gtgttccggc aacatcattc ggctcccttc ccacattaaa ttacctcttc 60 gtaaaaatat gtcctcgtgt tttttcatgg tgtcacctct ttataaccaa aatacaaaca 120 aacgttcgtg ttgtcaactg gttattgtta cctatataag aggtgtatgg aataggaatt 180 aaaaggaaag gaaaaaaggc gcctattttc gtaaaaaaat aggtaattac cagcatatcc 240 cgtgaaagaa tgctagttta gggtaagata taagccacat tctagtttgg ggagcagtag 300 attacggtgc taaatatata gtggtttaat gctttctaca tgttttttta gtgaattatt 360 attgagctat gtattcgtat ttaaagacat gtgtcattat ttcttataaa aagtaatcac 420 ttaaagtaaa acagcacttt cgcctagttt tcatgggatt gtcgtccgta taactggaaa 480 tgaaactaat attttcctgt attcagggta aaatgagaac aaaagaattt agttatttct 540 ttatctaatt tttgattcat tcttgggata aaataaccca tttttaaaat gaagatttga 600 agatagcaat agtgattgtc ttagaaaaaa gaagagctat aggaaaagac ggagggcagg 660 ggtagtattg aaatattttt cggaaaaggt ttaatagttg aagtggatga catcataata 720 gtttgctatt ctatgtagga aaatgaccat atatggatac agtatcataa gtgctatgac 780 ttttaactaa taaagaaaga cgataggtat attcttgaaa ttgactaaaa aaaaattgtt 840 ctatacaatg aattccgtta catattttgc aaaaaagagg gtgatggtgc tacatatact 900 ccttaaaaag tgcagactaa aacgatttcg ttgcagtatg aaaagctaaa ccattacaag 960 gaggaaaata tgaaaaagat aactactatt tttgtcgtat tgcttatgac agtggcgttg 1020 ttcagtatag gaaacacgac tgctgctgat aatgattcag ttgtagaaga acatgggcaa 1080 ttaagtatta gtaacggtga attagtcaat gaacgaggcg aacaagttca gttaaaaggg 1140 atgagttccc atggtttgca atggtacggt caatttgtaa actatgaaag tatgaaatgg 1200 ctaagagatg attggggaat aactgtattc cgagcagcaa tgtatacctc ttcaggagga 1260 tatattgatg atccatcagt caaggaaaaa gtaaaagagg ctgttgaagc tgcgatagac 1320 cttgatatat atgtaatcat tgattggcat atcctttcag acaatgaccc gaatatatat 1380 aaagaagaag cgaaagattt ctttgatgaa atgtcagagt tgtatggaga ctatccgaat 1440 gtgatatacg aaattgcaaa tgaaccgaat ggtagtgatg ttacgtggga caatcgaata 1500 aaaccgtatg cagaggaagt cattccggtt attcgtaaca atgaccctaa taacattatt 1560 attgtaggta caggtacatg gagtcaggat gtccatcatg cagctgataa tcagcttgca 1620 gatcctaacg tcatgtatgc atttcatttt tatgcaggga cacatggtca aaatttacga 1680 gaccaagtag attatgcatt agatcaagga gcagcgatat ttgttagtga atggggaaca 1740 agtgcagcta caggtgatgg tggcgtgttt ttagatgaag cacaagagtg gattgacttt 1800 atggatgaaa gaaatttaag ctgggcaaac tggtctttaa cgcataaaga tgagtcgtct 1860 gcagcgttaa tgccaggtgc aaacccaact ggtggttgga cagaggctga actatctcca 1920 tctggtacat ttgtgaggga aaaaataaga gaatcagcat ctattccgcc aagcgatcca 1980 acaccaccaa gtgatccagg agaatatcca gcatgggacc caacgcaaat ttacacaaat 2040 gaaattgtgt atcataacgg tcagttatgg caagcgaaat ggtggacaca aaatcaagag 2100 ccaggtgacc cgtacggtcc gtgggaacca ctcaattaaa ctatataatt gataaaaatt 2160 tactaatgag atagtgagaa tgccaagagt ctaaatttga agattggcat tctcatttta 2220 gaattaattt aatcattgaa aatatttaaa aacgaatttt ataatatcca aggtaccata 2280 cttaattggt ggtacttttt tctgtactta taactgccca tgccccgaaa aagttggtgc 2340 atttttctgc ataatattgt aaataccgaa ataagaaaaa gccttgaaac attgatataa 2400 caacgtttct aaggcttttt tgcatttctt attcagagta tgccaattaa cgagagtacc 2460 actcaacgat aagttgttcg ttaatttcag ctggaagttc agaacgctca ggtaaacgag 2520 tgaacgtacc ttcaagctt 2539 // |
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