EBI Dbfetch

ID   Z30237; SV 1; linear; genomic DNA; STD; PRO; 1499 BP.
XX
AC   Z30237;
XX
DT   01-JUL-1994 (Rel. 40, Created)
DT   18-APR-2005 (Rel. 83, Last updated, Version 7)
XX
DE   Serratia marcescens smeR and sme1 genes for lysR-type proteins
XX
KW   carbapenem-hydrolysing beta-lactamase; lysR-type protein; sme1 gene;
KW   smeR gene.
XX
OS   Serratia marcescens
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Serratia.
XX
RN   [1]
RP   1-222
RA   Naas T., Vandel L., Sougakoff W., Livermore D.M., Nordmann P.;
RT   "Cloning and sequence analysis of the carbapenem-hydrolysing class A
RT   beta-lactamase, BplA, from Serratia marcescens S6.";
RL   Unpublished.
XX
RN   [2]
RP   222-1499
RX   PUBMED; 7793864.
RA   Naas T., Livermore D.M., Nordmann P.;
RT   "Characterization of an LysR family protein, SmeR from Serratia marcescens
RT   S6, its effect on expression of the carbapenem-hydrolyzing beta-lactamase
RT   Sme-1, and comparison of this regulator with other beta-lactamase
RT   regulators";
RL   Antimicrob. Agents Chemother. 39(3):629-637(1995).
XX
RN   [3]
RP   1-1499
RA   Naas T.;
RT   ;
RL   Submitted (17-FEB-1994) to the EMBL/GenBank/DDBJ databases.
RL   Thierry Naas, Microbiology, Biozentrum, University of Basel,
RL   Klingelberstrasse 70, Basel, CH-4056, Switzerland
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1499
FT                   /organism="Serratia marcescens"
FT                   /strain="S6"
FT                   /mol_type="genomic DNA"
FT                   /clone="pPTN101"
FT                   /db_xref="taxon:615"
FT   CDS             243..1121
FT                   /transl_table=11
FT                   /gene="smeR"
FT                   /product="SmeR, LysR-type protein"
FT                   /db_xref="GOA:P52683"
FT                   /db_xref="InterPro:IPR000847"
FT                   /db_xref="InterPro:IPR005119"
FT                   /db_xref="InterPro:IPR011991"
FT                   /db_xref="UniProtKB/Swiss-Prot:P52683"
FT                   /protein_id="CAA82943.1"
FT                   /translation="MKNRIPLNALRAFEASARYLNFTKAGLELHVSQAAVSQHVRTLEA
FT                   ILGVNLFKRLPRGLQLTEEGLHLLPMINEAFSIMGSALERFHEGKIREVITVAVVGTFA
FT                   VGWLLPRLSGFTKSYPWIDIRVITHNNVINLAAEGIDAAIRFGHGFWQSTENYKLFSAP
FT                   HTVLCPPNVAKKLTTPEDMKDYRLLRTYRKEEWSSWFKAANLKPWPVTGPIFDSSRHMD
FT                   DAAKICGDIALAPYKMFIHEIENGSLVKPFDIEVHLGGYWLTILKSRSNIELNNALNIF
FT                   KTWLLNASHSI"
FT   mat_peptide     243..1118
FT                   /gene="smeR"
FT                   /product="SmeR, LysR-type protein"
FT   -35_signal      89..94
FT                   /gene="smeR"
FT   -10_signal      122..127
FT                   /gene="smeR"
FT   mRNA            125..>1121
FT                   /gene="smeR"
FT   RBS             233..238
FT                   /gene="smeR"
FT                   /note="putative"
FT   CDS             complement(<1..95)
FT                   /transl_table=11
FT                   /gene="sme1"
FT                   /product="carbapenem-hydrolysing beta-lactamase"
FT                   /db_xref="GOA:P52682"
FT                   /db_xref="InterPro:IPR000871"
FT                   /db_xref="InterPro:IPR001466"
FT                   /db_xref="InterPro:IPR012338"
FT                   /db_xref="UniProtKB/Swiss-Prot:P52682"
FT                   /protein_id="CAA82944.1"
FT                   /translation="MSNKVNFKTASFLFSVCLALSAFNAHANKSDA"
FT   -35_signal      complement(194..199)
FT                   /gene="sme1"
FT   -10_signal      complement(172..177)
FT                   /gene="sme1"
FT   mRNA            complement(<1..164)
FT                   /gene="sme1"
FT   RBS             complement(100..106)
FT                   /gene="sme1"
FT                   /note="putative"
XX
SQ   Sequence 1499 BP; 475 A; 269 C; 267 G; 488 T; 0 other;
     gcatcacttt tgttagcatg agcattaaat gccgacaaag ctaaacaaac actaaacaaa        60
     aatgaagccg ttttaaaatt tactttgttt gacataactt tacctattaa ctgtcattgt       120
     atagaatgag cttcattact aacggagtgt tgtaatgagt tgagaaaacg aattatacag       180
     ttatagatgt tgatttaaat ctataaaatt tactatctgc cataagttta tcttttgatt       240
     ctatgaaaaa tcgcatacca cttaatgcat tacgtgcttt tgaagcatcg gcacgttatt       300
     taaactttac taaagcaggg cttgaactac atgtaagcca agcagctgtt agtcagcatg       360
     ttcggactct tgaggcaatc cttggggtaa acctgtttaa aaggctacct cgtggtttac       420
     aactcacaga agaaggtctg catctattac cgatgattaa tgaagcattt agcatcatgg       480
     gctcagcgct ggaacggttt catgaaggaa aaattcgcga ggtaataact gttgctgtcg       540
     taggaacgtt cgcagttggt tggttactac caagattgag tgggtttaca aaaagttacc       600
     catggataga catcagagtt attactcata ataatgtcat taatttggcc gctgaaggaa       660
     ttgatgcagc aattcgtttc ggtcatggtt tttggcaatc aactgaaaac tataagttgt       720
     tttccgcccc gcatactgtg ctatgccctc cgaatgttgc aaagaaactt accacccctg       780
     aggatatgaa agattatcgt ctactacgta catacagaaa ggaggagtgg agtagttggt       840
     tcaaggctgc aaaccttaag ccctggccgg taactggacc aattttcgat tcttcaagac       900
     atatggatga tgcagctaaa atatgtggcg acatcgcatt agcaccatat aaaatgttta       960
     tacatgaaat agaaaatggt tctctcgtta aaccttttga tatagaggtt catttgggag      1020
     gatattggct cactatttta aaatctcgtt ctaacattga gctaaacaat gcattaaata      1080
     ttttcaaaac atggttacta aatgcctccc attctatcta aaagcctatt cgatatggcc      1140
     agaataataa atttacttaa gtaatttggt gtcgaatgag cgccctacaa agagcgcttc      1200
     aatttaacct ttgatcagca aagttcatta ataccaatca tgccttacca aacgtttttg      1260
     tcgttagtgc aataaacatg atttgtcatc attattttgt taatgaattt atttttcctt      1320
     gctaccattc taattagata gcttattctt tgctcgctca aaccagttaa tacacttatc      1380
     tctttataag aaagcatgtt gacaaaataa taaaaaaaga caatattttt ctgattttca      1440
     atagccgtta ttttagtctt tgattttctt tgaaaaacct ccccgatatt attaagctt       1499
//