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EBI DbfetchID Z25795; SV 1; linear; genomic DNA; STD; PRO; 8517 BP. XX AC Z25795; XX DT 27-AUG-1993 (Rel. 36, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 10) XX DE B.subtilis glycogen operon DNA XX KW 1,4-alpha-glucan branching enzyme; ADP-glucose pyrophosphorylase; KW glgA gene; glgB gene; glgC gene; glgD gene; glgP gene; KW glucose-1-phosphate adenylyl transferase; glycogen phosphorylase; KW starch synthase; transfer RNA-Asn; transfer RNA-Glu; transfer RNA-Ser. XX OS Bacillus subtilis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-8517 RX DOI; 10.1111/j.1365-2958.1994.tb00301.x RX PUBMED; 8145641. RA Kiel J.A., Boels J.M., Beldman G., Venema G.; RT "Glycogen in Bacillus subtilis: molecular characterization of an operon RT encoding enzymes involved in glycogen biosynthesis and degradation"; RL Mol. Microbiol. 11(1):203-218(1994). XX RN [2] RC (sites) RX PUBMED; 6323435. RA Wawrousek E.F., Narasimhan N., Hansen J.N.; RT "Two large clusters with thirty-seven transfer RNA genes adjacent to RT ribosomal RNA gene sets in Bacillus subtilis. Sequence and organization of RT trrnD and trrnE gene clusters"; RL J. Biol. Chem. 259(6):3694-3702(1984). XX RN [3] RC (sites) RX DOI; 10.1016/0378-1119(86)90090-9 RX PUBMED; 3104146. RA Hay R., Tatti K., Vold B., Green C., Moran Jr.C.; RT "Promoter used by sigma-29 RNA polymerase from Bacillus subtilis"; RL Gene 48(2-3):301-306(1986). XX RN [4] RP 1-8517 RA Kiel J.; RT ; RL Submitted (25-AUG-1993) to the EMBL/GenBank/DDBJ databases. RL Kiel J., State University of Groningen, Center of Biol. Sciences, Dept. of RL Genetics, Kerklaan 30, Haren, Groningen, The Netherlands, NL-9751 NN XX DR RFAM; RF00005; tRNA. XX FH Key Location/Qualifiers FH FT source 1..8517 FT /organism="Bacillus subtilis" FT /strain="168" FT /isolate="0G1" FT /mol_type="genomic DNA" FT /clone_lib="Lambda EMBL12 genebank" FT /clone="pKBU1 and pKBU101" FT /db_xref="taxon:1423" FT tRNA <1..40 FT /product="tRNA-Asn" FT /note="part of trnB region; nt 1-418 also sequenced by FT Green CJ and Vold BS (1983). Sequence analysis of a cluster FT of twenty-one tRNA genes in Bacillus subtilis. Nucleic FT Acids Res. 16:5763-5774 (citation 4)." FT /citation=[1] FT tRNA 44..134 FT /product="tRNA-Ser" FT /anticodon=(pos:78..80,aa:Ser) FT /note="part of trnB region; also listed in (citation 4)" FT /citation=[1] FT tRNA 160..231 FT /product="tRNA-Glu" FT /anticodon=(pos:193..195,aa:Glu) FT /note="part of trnB region; also listed in citation 4" FT /citation=[1] FT stem_loop 235..263 FT /function="terminator" FT /label=T1 FT /note="putatitive terminator downstream trnB; also listed FT in (citation 4)" FT /citation=[1] FT stem_loop 381..402 FT /function="terminator" FT /label=T2 FT /note="putative terminator downstream from trnB; also FT listed in (citation 4)" FT /citation=[1] FT -35_signal 417..424 FT /note="-35 region of bvx promoter recognized by Bacillus FT subtilis RNA polymerase containing the sporulation sigma FT factor E" FT /citation=[1] FT -10_signal 438..445 FT /note="-10 region of bvx promoter recognized by Bacillus FT subtilis RNA polymerase containing the sigma factor E." FT /citation=[1] FT RBS 448..460 FT /citation=[1] FT CDS 464..2347 FT /transl_table=11 FT /gene="glgB" FT /product="1,4-alpha-glucan branching enzyme" FT /function="introduces alpha-1,6-linkages in starch and FT glycogen" FT /EC_number="2.4.1.18" FT /note="first gene of glycogen operon" FT /db_xref="GOA:P39118" FT /db_xref="HSSP:1M7X" FT /db_xref="InterPro:IPR013783" FT /db_xref="UniProtKB/Swiss-Prot:P39118" FT /citation=[1] FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA81040.1" FT /translation="MAAASPTAHDVYLFHEGSLFKSYQLFGSHYRELNGKSGYEFCVWA FT PHASEVRVAGDFNSWSGEEHVMHRVNDNGIWTLFIPGIGEKERYKYEIVTNNGEIRLKA FT DPYAIYSEVRPNTASLTYDLEGYSWQDQKWQKKQKAKTLYEKPVFIYELHLGSWKKHSD FT GRHYSYKELSQTLIPYIKKHGFTHIELLPVYEHPYDRSWGYQGTGYYSPTSRFGPPHDL FT MKFVDECHQQNIGVILDWVPGHFCKDAHGLYMFDGEPLYEYKEERDRENWLWGTANFDL FT GKPEVHSFLISNALYWAEFYHIDGFRVDAVANILYWPNQDERHTNPYAVDFLKKLNQTM FT REAYPHVMMIAEDSTEWPQVTGAVEEGGLGFHYKWNMGWMNDVLKYMETPPEERRHCHQ FT LISFSLLYAFSEHFVLPFSHDEVVYGKKSLLNKMPGDYWQKFAQYRLLLGYMTVHPGKK FT LIFMGSEFAQFDEWKDTEQLDWFLDSFPMHQKASVFTQDLLRFYQKSKILYEHDHRAQS FT FEWIDVHNDEQSIFSFIRYGQKHGEALVIICNFTPVVYHQYDVGVPFFTQYIEVLNSDS FT ETYGGSGQINKKPLSAKKGALHHKPCYITMTIPPYGISILRAVKKRGEIKR" FT RBS 2327..2337 FT /citation=[1] FT CDS 2344..3486 FT /transl_table=11 FT /gene="glgC" FT /product="ADP-glucose pyrophosphorylase" FT /function="activates glucose-1-phosphate using ATP" FT /EC_number="2.7.7.27" FT /note="second gene in glycogen operon" FT /db_xref="GOA:P39122" FT /db_xref="InterPro:IPR005835" FT /db_xref="UniProtKB/Swiss-Prot:P39122" FT /citation=[1] FT /protein_id="CAA81041.1" FT /translation="MKKQCVAMLLAGGKGSRLSGLTKNMAKPAVSFGGKYRIIDFTLSN FT CSNSGIDTVGILTQYQPLELNSYIGIGSAWDLDRYNGGVTVLPPYAESSEVKWYKGTAS FT SIYENLNYLNQYDPEYVLILSGDHIYKMDYGKMLDYHIEKKADVTISVIEVGWEEASRF FT GIMKANPDGTITHFDEKPKFPKSNLASMGIYIFNWPLLKQYLEMDDQNPYSSHDFGKDI FT IPLLLEEKKKLSAYPFKGYWKDVGTVQSLWEANMDLLKEDSELKLFERKWKIYSVNPNQ FT PPQFISSDAQVQDSLVNEGCVVYGNVSHSVLFQGVTVGKHTTVTSSVIMPDVTIGEHVV FT IENAIVPNGMVLPDGAVIRSEKDIEEVLLVSEEFVEKELI" FT RBS 3494..3501 FT /citation=[1] FT CDS 3510..4541 FT /transl_table=11 FT /gene="glgD" FT /product="protein with similarity to ADP-glucose FT pyrophosphorylase" FT /function="unknown" FT /note="third gene in glycogen operon" FT /db_xref="GOA:P39124" FT /db_xref="InterPro:IPR005835" FT /db_xref="UniProtKB/Swiss-Prot:P39124" FT /citation=[1] FT /protein_id="CAA81042.1" FT /translation="MFNNQMLGVIDETTYKHSLQDLTAQRSLGAIPFAGRYRLIDFMLS FT NMVNADIRSVAIFPKYRYRSLMDHLGAGKEWDLHRKKDGLFFFPSPHLHHEYDEFGSFR FT QFSDHLDYFHRSTQQYAVISNSHTVCNIQFQYVLKRHQEVGCDVTEVFQDGQSLQIYIM FT STTLLKDLIYGHSEKGYKTIQEAVEKESSALTICPYEYSGYAAVIDSVEKYYTHSMELI FT QPRFWQQVFLPQQPIYTKVKDEPPTKYGKHSTVKNSLVANGCVLEGEVENCILFRAVHV FT GKGTKLKNCIIMQKTQIGEDCLLEQVISDKDVKIGKATEAAGTAEQPLVLRKGLVQGEL FT MNS" FT RBS 4519..4531 FT /citation=[1] FT CDS 4538..5992 FT /transl_table=11 FT /gene="glgA" FT /product="starch (bacterial glycogen) synthase" FT /function="synthesizes alpha-1,4-glucan chains using FT ADP-glucose" FT /EC_number="2.4.1.21" FT /note="fourth gene in glycogen operon" FT /db_xref="GOA:P39125" FT /db_xref="InterPro:IPR013534" FT /db_xref="UniProtKB/Swiss-Prot:P39125" FT /citation=[1] FT /protein_id="CAA81043.1" FT /translation="MKILFAVSECTPFVKSGGLADVAGALPKALARLGNEVAVMLPKYS FT QIPEPWKKRMKKQAECTVAVGWRQQYCGIEHMAENDVNYYFIDNEYYFNRDSLYGHYDD FT GERFAFFSRAVLEAAKVVNVQADIVHTHDWHTAMVNYLLKEEYRKHPFYERMKSVLTIH FT NLQFQGIFPPDVTHDLLGLEMDHFHYERLECNGFVNFMKAGIIAADHVTTVSPTYRNEI FT MTPYYGEQLEQVLQYREDDVTGILNGIDDTFYQPKSDPYIEAQYDSGDLACKLENKTKL FT QQRMGLPEKNDIPLISMVTRLTKQKGLDLVRRIMHELLEEQDIQLVVLGTGEREFEDYF FT RYAEFAFHEKCRAYIGFDEPLAHQIYAGSDMFLMPSKFEPCGLGQLIALQYGAIPIVRE FT TGGLYDTVRAYQEEEGTGNGFTFSAFNAHDLKFTIERALSFYCQQDVWKSIVKTAMNAD FT YSWGKSAKEYQRIFEQVTRSGRDVLE" FT RBS 5965..5974 FT /citation=[1] FT CDS 5979..8375 FT /transl_table=11 FT /gene="glgP" FT /product="Glycogen Phosphorylase" FT /function="degrades starch and glycogen by phosphorylation" FT /note="fifth and last gene of glycogen operon" FT /db_xref="GOA:P39123" FT /db_xref="HSSP:1YGP" FT /db_xref="InterPro:IPR000811" FT /db_xref="UniProtKB/Swiss-Prot:P39123" FT /citation=[1] FT /protein_id="CAA81044.1" FT /translation="MFSSKERFADLFLKRLEMTCGKSFKDSAKLDQYKTLGNMVREYIS FT ADWIETNEKSRSNSGKQTYYLSIEFLLGQLLEQNLMNLGVRDVVEAGLKEIGINLEEIL FT QIENDAGLGNGGLGRLAACFLDSLASLNLPGHGMGIRYKHGLFEQKIVDGHQVELPEQW FT LKNGNVWEVRNADQAVDVPFWGEVHMTEKSGRLHFRHEQATIVTAVPYDIPIIGYETGT FT VNTLRLWNAEPYAHYHGGNILSYKRETEAVSEFLYPDDTHDEGKILRLKQQYFLVCASL FT KSIVNNYRKTHKSLSGLHKKVSIHINDTHPALAVPELMRILLDEENMSWEEAWHITVHT FT ISYTNHTTLSEALEKWPIHLFKPLLPRMYMIIEEINERFCRAVWEKYPGDWKRIENMAI FT TAHGVVKMAHLAIVGSYSVNGVAKIHSDILKEREMRDFHLLFPNRFNNKTNGIAHRRWL FT LKANPGLSAIITEAIGDEWVKQPESLIRLEPYATDPAFIEQFQNNKSKKKQELADLIFC FT TAGVVVNPESIFDVQVKRLHAYKRQLLNVLHIMYLYNRLKEDSGFSIYPQTFIFGAKAS FT PSYYYAKKIIKLIHSVAEKVNYDPAVKQLIKVVFLENYRVSMAERIFPASDVSEQISTA FT SKEASGTGNMKFMMNGALTIGTHDGANIEILERVGPDCIYTFGLKADEVLSYQENGGYR FT SREYYQHDRRIRQVADQLINGFFEGEADEFESIFDSLLPHNDEYFVLKDFSSYADAQER FT IQADYRERRKWSEHSIVNIAHSGYFSSDRTIREYAKDIWGIKPMM" FT stem_loop 8375..8401 FT /function="terminator" FT /note="putative terminator of glycogen operon" FT /citation=[1] XX SQ Sequence 8517 BP; 2465 A; 1629 C; 2115 G; 2308 T; 0 other; aaccgatcgg tcgcaggttc gaatcctgcc tgtggagcca aatggagaag tactcaagtg 60 gctgaagagg cgcccctgct aagggtgtag gtcgtgtaag cggcgcgagg gttcaaatcc 120 ctccttctcc gccatatgat tacagatatc ataattatcg gcccgttggt caagcggtta 180 agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc cgtacgggtc atccagaagc 240 cttgcatatc ctgcaaggtt tttttgtttt tataaatcat gatatgtctt agattttgtt 300 ctttatttta aaaacagact acaaaaatct ccatatattt cgtttttctt cagaaaatga 360 agttaattgt ctataagtat aagcgctttc aggaaagggc ttttttttat ttcttcgaat 420 aaatactata aatgaaaact atgatgtcag aaaggatgat tacatggccg ctgccagccc 480 gacagcacat gatgtttatc tttttcacga aggcagtctg tttaaaagct atcagctgtt 540 tggctcgcat taccgcgagc tgaatggcaa aagcggatat gaattctgtg tgtgggcgcc 600 tcatgcgtcg gaagtacgag tggctggaga ttttaacagc tggtctggag aagagcatgt 660 gatgcacaga gtgaatgata atggcatttg gacgttattt attccaggta taggagaaaa 720 ggaacgatat aaatacgaaa ttgtgacgaa taacggtgag atcaggctga aggcagatcc 780 atacgctatt tattcagaag tcagaccaaa cacagcgtca cttacttacg atttggaagg 840 atacagctgg caagatcaaa aatggcaaaa gaaacagaag gctaagacgc tgtatgaaaa 900 acctgttttc atttatgaac tgcatcttgg gtcttggaag aagcattctg atggcaggca 960 ttattcctat aaagaattaa gtcagacgct tatcccttat atcaagaagc atgggtttac 1020 ccatattgaa ctgcttcccg tgtatgagca tccatatgat cgttcatggg ggtaccaagg 1080 aacggggtat tacagcccga caagcaggtt tggcccgccg catgatctga tgaagtttgt 1140 ggatgaatgt catcaacaaa atatcggagt cattttggat tgggtccccg gacatttttg 1200 taaagatgca catggccttt atatgtttga tggagaaccg ctttatgaat acaaagaaga 1260 acgtgatcgg gagaactggt tatggggcac ggcaaacttt gacctgggga aaccggaagt 1320 tcatagtttt ttaatttcga atgcgttgta ctgggcggaa ttctatcata tagatggatt 1380 tagggttgat gcagtggcta atatcttgta ttggccaaat caggatgaac gccacacgaa 1440 tccatatgct gttgattttt taaaaaaact caatcaaacc atgagagagg catatcccca 1500 tgtgatgatg attgcagaag attctacaga atggcctcaa gtgacaggtg ccgttgagga 1560 aggggggctt ggttttcatt ataaatggaa tatgggatgg atgaatgacg tactgaaata 1620 catggagacg ccgcccgaag aaaggcggca ttgtcatcag cttatctctt tttcattact 1680 atatgcgttt agtgaacatt ttgtattgcc gttttctcat gatgaagtcg tatacggaaa 1740 aaaatcactt ctcaataaaa tgcctggcga ctactggcag aaatttgccc agtatcggct 1800 tttgctgggc tatatgacag tgcatcccgg aaagaaattg atctttatgg gatcagagtt 1860 tgctcaattt gatgaatgga aagatacaga acagcttgat tggtttttgg actccttccc 1920 catgcatcag aaagccagcg tttttacgca agatcttctc cgcttttacc aaaaaagcaa 1980 aatcctttat gagcatgacc atcgtgcaca atcgtttgaa tggattgatg tccataatga 2040 cgaacaatca atcttttctt ttattcgtta tggccaaaag cacggtgagg cgcttgtcat 2100 catttgtaat tttacacccg tggtctatca tcaatatgat gttggagttc ctttttttac 2160 tcaatacatt gaagtgctta acagtgacag tgaaacatat ggaggctcag ggcaaatcaa 2220 caaaaagccg ttatcggcta aaaagggagc acttcatcat aaaccgtgtt atatcacgat 2280 gaccatcccc ccttacggca tctcgatttt acgggcggtt aaaaaaagag gagagataaa 2340 aagatgaaaa aacaatgtgt agccatgctc cttgccggcg ggaagggcag ccgtctcagc 2400 ggattaacaa aaaatatggc caaaccggct gtatctttcg ggggaaaata caggattatt 2460 gattttaccc taagcaactg ttctaattca ggcatagaca cagtcgggat tttaacgcaa 2520 tatcagccgc tcgagctgaa ttcctatatt gggatcggca gtgcatggga cctggataga 2580 tataatggcg gcgtgacggt tttaccccct tatgccgagt catcagaagt caaatggtat 2640 aaagggacgg caagctccat ttatgaaaat ctcaattatt tgaatcagta tgatcctgag 2700 tatgtactga ttttatcagg agatcacata tacaaaatgg actacggcaa aatgcttgat 2760 tatcacattg aaaagaaagc agatgtgacc atctctgtca tcgaagtggg ctgggaggaa 2820 gcaagccggt tcggcatcat gaaggccaat ccagatggga cgatcacaca ttttgatgaa 2880 aagcccaaat tccctaagag caatcttgcc tcaatgggaa tttatatatt caattggccg 2940 cttttgaaac agtatcttga gatggacgat caaaaccctt attctagcca cgacttcggc 3000 aaagacatta ttccactgct tttggaagag aaaaagaagc tttctgctta tccgttcaag 3060 ggatattgga aggatgtcgg aacggtgcag agcttgtggg aagccaatat ggatttgtta 3120 aaagaggact cggagctgaa gctgtttgaa cgcaaatgga aaatttactc cgtcaatcca 3180 aatcagcctc cgcaatttat ttcatctgat gcgcaggttc aagattcgct tgtgaacgaa 3240 ggttgtgttg tatatggaaa tgtgtctcac tccgttcttt ttcaaggtgt gactgttggt 3300 aagcatacga ctgttacatc atccgtcata atgccggatg tgacgattgg ggagcatgta 3360 gtcattgaaa atgccatcgt tcctaatggg atggtgctgc cggatggcgc agtgattcgc 3420 tccgaaaaag acattgaaga ggtgctgctc gtttcagagg aatttgtaga aaaagaattg 3480 atttaattac tgaagagggg gcagagtcag tgttcaataa tcaaatgtta ggtgttatag 3540 atgaaaccac atataagcat tctcttcagg atttaacagc acagcggtcg ctcggggcga 3600 tcccttttgc cggcaggtac cgactgattg attttatgct ttccaacatg gtgaatgcgg 3660 atattaggag tgttgcaatt tttccgaagt atcgttaccg ttcgctcatg gatcaccttg 3720 gggcgggtaa ggaatgggat ttgcaccgga aaaaggacgg cctctttttt ttcccgtctc 3780 cccatcttca tcacgaatac gatgaattcg gttcctttcg ccagttttcg gaccatctag 3840 attacttcca cagaagcact cagcaatatg ctgtaatctc caacagccat accgtatgca 3900 acattcaatt tcaatatgtg ctgaaacgcc atcaggaggt tggctgcgac gtcaccgagg 3960 tatttcagga cggccagtcg ctgcaaatct atatcatgtc aacaacgctt ttaaaggatc 4020 tgatctacgg acatagcgaa aaaggctata aaaccattca ggaggctgtt gaaaaggaat 4080 cttccgccct gaccatttgc ccttatgaat attcaggtta tgccgctgtc attgattcgg 4140 tggaaaaata ttatacccac agtatggagc tcattcagcc tcgtttttgg cagcaggtgt 4200 ttttgccgca gcagccgatc tatacaaaag tgaaggatga gccgcctacg aaatacggaa 4260 agcacagcac tgtcaaaaat tctttggtgg caaacggctg tgtgcttgaa ggagaggttg 4320 aaaactgcat tttgttcagg gcggtccatg tcggaaaggg tacgaagctg aaaaactgca 4380 ttattatgca aaaaacgcag attggagagg attgtcttct tgagcaggtc atttcggata 4440 aggatgttaa aatcggcaag gcgacggaag ctgcaggaac agctgagcag ccgcttgtat 4500 taagaaaagg tttggtgcaa ggagagctga tgaattcttg aagatattat ttgctgtgtc 4560 agaatgcacc ccatttgtga aatcaggagg tcttgccgat gtagcaggtg ctctgccaaa 4620 ggcgcttgcg cgattaggca atgaggtagc tgtgatgctg ccgaagtaca gccaaatacc 4680 tgaaccgtgg aaaaagcgta tgaaaaaaca ggctgagtgt accgtagctg tcggctggcg 4740 gcagcaatat tgcggaattg aacatatggc agagaatgat gtgaattatt acttcatcga 4800 caatgagtac tatttcaaca gggattcgtt gtacgggcat tatgatgacg gtgagcggtt 4860 tgcctttttt tcaagagctg tgctggaggc tgccaaagtt gtgaatgttc aagccgatat 4920 tgttcatact cacgattggc atacagcgat ggtcaattat ttgctgaaag aggagtacag 4980 aaaacatcct ttttatgaac ggatgaaaag cgtgctcacg atccacaacc tgcagtttca 5040 aggcatattc cctcctgatg tcacacatga cttgctgggt cttgagatgg atcattttca 5100 ttatgaaagg ttagaatgca acggatttgt taattttatg aaagcgggca tcattgcggc 5160 tgatcatgtg acaactgtga gccccactta cagaaatgaa atcatgacgc cgtattatgg 5220 tgagcagttg gaacaggttt tgcaatatag ggaagatgat gtaaccggca tattaaatgg 5280 cattgatgat acgttctatc agccgaaaag cgacccttat atagaggcgc aatacgattc 5340 aggggatctt gcatgcaagc tggaaaacaa aacgaagctt cagcagcgga tggggcttcc 5400 agagaaaaac gatattccgc tgatcagtat ggtgacaagg ctcactaaac aaaaggggct 5460 tgatcttgtc cgcagaatta tgcacgaact tcttgaggag caagacatac agctggttgt 5520 gctaggcaca ggagaacgtg aatttgaaga ttatttccgg tatgcagaat ttgcttttca 5580 tgagaagtgc cgggcttata tcgggtttga tgagccgcta gcccatcaaa tttatgcggg 5640 ctctgatatg tttttgatgc cgtcgaaatt tgagccgtgc ggtttggggc agctcattgc 5700 tcttcaatat ggcgccattc cgattgtcag ggaaacgggc ggtctttacg acactgtccg 5760 tgcctatcag gaggaagagg gaacgggcaa tggctttacg ttttccgcct ttaatgcgca 5820 tgatctgaaa tttacgatag aaagggcgct gtcgttttat tgccaacagg atgtatggaa 5880 gagcatcgtg aagaccgcta tgaatgcaga ttacagctgg gggaagtcgg ctaaagagta 5940 tcagcgtatt ttcgagcagg tgacaaggag tggacgggat gttctcgagt aaagaacgct 6000 ttgcagactt atttttaaag cgtctggaaa tgacgtgcgg aaaaagcttt aaagattctg 6060 ccaagctgga tcaatataaa acgttgggca atatggtcag agagtatatc agcgctgatt 6120 ggatcgaaac gaatgaaaaa agcagatcaa attccgggaa gcagacatat tatttatcaa 6180 tcgaatttct gctcggtcag ctccttgagc aaaacttaat gaatcttggt gttcgtgatg 6240 ttgtagaagc tggtttaaag gaaatcggca tcaatttaga ggagatcctt cagatagaga 6300 acgatgcagg tctcggcaat ggcggacttg ggcgcttggc cgcttgcttt ttagattcgc 6360 ttgcttcgtt gaacctgcct gggcacggca tgggcatacg ttataagcat ggtttgtttg 6420 agcaaaaaat tgtagacgga catcaggtgg agctgcctga gcaatggctg aaaaacggga 6480 acgtatggga agtaaggaat gcggatcaag cagtggacgt gccgttttgg ggcgaagtcc 6540 atatgacaga aaaaagcggg cgcctgcatt ttcgccatga acaagcgaca attgttaccg 6600 ctgttcctta tgatatcccg atcatcggct atgaaacggg cactgtcaac acgttaaggc 6660 tttggaacgc cgagccctat gcccattatc acggcggtaa tattttgtct tataagcgag 6720 aaacagaagc ggtttctgaa ttcttatatc ccgatgacac ccatgatgag gggaaaattt 6780 tacggctgaa gcagcagtat tttttagtat gcgcaagctt aaaaagcatt gtgaacaatt 6840 accgtaaaac acataaatct ttatccggcc tgcataaaaa agtgagcatt catattaatg 6900 acactcaccc tgcgcttgcg gtgcctgagc taatgcggat tttgctcgat gaagagaaca 6960 tgagctggga agaagcttgg catatcactg tacatacgat ttcttataca aatcatacaa 7020 cattgtcaga agcgctcgag aaatggccga ttcatttgtt taagccgctg ctcccgcgaa 7080 tgtacatgat tattgaagag atcaatgaga ggttttgccg tgcagtttgg gaaaagtatc 7140 ctggagactg gaaaaggata gagaacatgg cgattacagc gcatggtgtt gtcaaaatgg 7200 ctcatctggc catagtaggc agctacagtg taaatggcgt tgcaaaaata cattccgata 7260 tattaaagga aagggagatg cgcgactttc acttgctgtt tccgaatcgt tttaacaata 7320 agacgaatgg aattgcccac aggcgctggc tgttaaaagc gaatccaggc ttgtctgcca 7380 tcattacaga ggcgatcggc gatgaatggg tcaagcagcc ggaatcactg atccgtttag 7440 aaccgtacgc cactgatcct gcatttattg aacagtttca aaacaacaaa agcaagaaaa 7500 aacaagagct tgccgatttg attttttgca cggccggggt ggttgtgaac cctgagagca 7560 tattcgacgt acaggtgaaa cggcttcatg catataaacg gcagctgctg aatgttcttc 7620 acattatgta tttgtataac cgcctcaagg aggattcggg gttttcaatc tacccgcaaa 7680 cctttatttt cggggcaaag gcttcaccaa gctattatta tgccaagaaa atcattaagc 7740 tgatccattc tgtcgcagaa aaagtcaatt atgatccggc ggtcaaacag ctgatcaaag 7800 ttgttttttt agaaaattat agagtttcta tggctgagcg aatttttccg gcatcagacg 7860 taagtgagca aatttcaacc gcaagcaagg aagcgtctgg tacagggaat atgaagttta 7920 tgatgaacgg ggctttgacg atcggcacgc atgacggcgc aaatatcgag atccttgagc 7980 gggtgggccc tgactgtatt tacacgttcg gcttaaaggc ggatgaggtt ctatcgtatc 8040 aggaaaacgg cggctatagg tcgagggaat attatcagca tgacaggaga atcaggcagg 8100 tagcggatca gttgatcaat ggtttttttg aaggagaggc agatgaattt gaatcgatct 8160 ttgactcgct gcttccgcat aatgatgagt attttgtgct gaaggatttc agctcatatg 8220 cagatgcaca ggagcgaatt caagctgatt atcgagagcg aaggaaatgg tctgagcatt 8280 cgattgtaaa cattgctcat tctggatatt tctccagcga ccgcacgatc cgggaatatg 8340 cgaaggacat ttggggcatc aagccgatga tgtgaaaaag ccgctgtata agcggctttt 8400 tttgttcaca gattcattga attgattgta aaggataaga acgtcgcaaa tgcactccat 8460 aaaaaataag gcaaaagaag atagcttgcg gctctgctgt attttttggc aatgatc 8517 // |
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