spacer
spacer
EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   Z25544; SV 1; linear; genomic DNA; STD; PRO; 1471 BP.
XX
AC   Z25544;
XX
DT   29-APR-1994 (Rel. 39, Created)
DT   14-NOV-2006 (Rel. 89, Last updated, Version 5)
XX
DE   B.stearothermophilus genes for alcohol dehydrogenase and aldehyde
DE   dehydrogenase (partial)
XX
KW   alcohol dehydrogenase; aldehyde dehydrogenase.
XX
OS   Geobacillus stearothermophilus
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Geobacillus.
XX
RN   [1]
RP   1-1471
RX   DOI; 10.1016/0167-4781(94)90199-6
RX   PUBMED; 8049268.
RA   Robinson G.A., Bailey C.J., Dowds B.C.;
RT   "Gene structure and amino acid sequences of alcohol dehydrogenases of
RT   Bacillus stearothermophilus";
RL   Biochim. Biophys. Acta 1218(3):432-434(1994).
XX
RN   [2]
RP   1-1471
RA   Bailey C.J.;
RT   ;
RL   Submitted (18-AUG-1993) to the EMBL/GenBank/DDBJ databases.
RL   Christopher J. Bailey, Trinity College Dublin, Department of, Biochemistry,
RL   Trinity College, Dublin 2, Dublin, Ireland
XX
DR   CABRI; DSM 2334.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1471
FT                   /organism="Geobacillus stearothermophilus"
FT                   /strain="DSM 2334"
FT                   /variety="non-diastaticus"
FT                   /mol_type="genomic DNA"
FT                   /clone="clone pADH2"
FT                   /db_xref="taxon:1422"
FT   terminator      126..147
FT   -35_signal      179..184
FT   -10_signal      199..204
FT   RBS             217..224
FT   CDS             234..1253
FT                   /transl_table=11
FT                   /transl_except=(pos:234..236,aa:Met)
FT                   /product="alcohol dehydrogenase"
FT                   /EC_number="1.1.1.1"
FT                   /label=ADH
FT                   /db_xref="GOA:P42327"
FT                   /db_xref="HSSP:1JVB"
FT                   /db_xref="InterPro:IPR002328"
FT                   /db_xref="UniProtKB/Swiss-Prot:P42327"
FT                   /protein_id="CAA80989.1"
FT                   /translation="MKAAVVNEFKKALEIKEVERPKLEEGEVLVKIEACGVCHTDLHAA
FT                   HGDWPIKPKLPLIPGHEGVGIVVEVAKGVKSIKVGDRVGIPWLYSACGECEYCLTGQET
FT                   LCPHQLNGGYSVDGGYAEYCKAPADYVAKIPDNLDPVEVAPILCAGVTTYKALKVSGAR
FT                   PGEWVAIYGIGGLGHIALQYAKAMGLNVVAVDISDEKSKLAKDLGADIAINGLKEDPVK
FT                   AIHDQVGGVHAAISVAVNKKAFEQAYQSVKRGGTLVVVGLPNADLPIPIFDTVLNGVSV
FT                   KGSIVGTRKDMQEALDFAARGKVRPIVETAELEEINEVFERMEKGKINGRIVLKLKED"
FT   CDS             1253..>1471
FT                   /transl_table=11
FT                   /product="aldehyde dehydrogenase"
FT                   /EC_number="1.2.1.3"
FT                   /label=ALDH
FT                   /note="Partial putative sequence"
FT                   /db_xref="GOA:P45959"
FT                   /db_xref="HSSP:1BI9"
FT                   /db_xref="InterPro:IPR015590"
FT                   /db_xref="UniProtKB/Swiss-Prot:P45959"
FT                   /protein_id="CAA80990.1"
FT                   /translation="MMSSIAAPKLKEKVEKFLSGKKKMYINGSFVESASGKTFDTPNPA
FT                   TGERLATVYEGDAEDIDRAVKAAREAFD"
XX
SQ   Sequence 1471 BP; 478 A; 243 C; 355 G; 395 T; 0 other;
     gatcattcat gcttgagcaa aaactgaaca ctatattttt agcaggtttc caactggaac        60
     aacctgcttt ttcttttcat gcaacaattc tgccaatatt ttgttcaagt ctgccaaaaa       120
     ttcggcaaaa ttctattttg tatttttcta atattccgca aattaagcgc tttcaatttt       180
     ggtacggttc ttgcatgata taaagtcgaa aaaaatgaaa ggggttgatt tgagtgaaag       240
     cagcagtagt taacgaattt aaaaaagcac ttgaaattaa agaggtggaa agacccaaac       300
     tagaggaagg tgaagtttta gttaagatcg aagcatgcgg tgtgtgccat acggatttac       360
     atgcagccca tggtgactgg ccgattaaac cgaaattgcc gttaattccg gggcatgaag       420
     gagtcggaat tgttgtagaa gtcgcaaagg gagttaaatc aataaaagtg ggagatcgcg       480
     tcggcatccc atggttatat tctgcatgtg gtgaatgtga atattgttta actggtcaag       540
     aaacactttg cccgcatcaa ttgaatggcg gatattccgt tgatggcgga tatgcggaat       600
     actgtaaagc tcccgcagat tatgtggcga aaattcctga taacttagat ccagttgaag       660
     tagcgccaat tttatgtgca ggcgtaacga catataaagc gttgaaagta tcaggtgctc       720
     ggccaggtga atgggtggcc atttacggta ttggaggact tggtcatatt gcattacaat       780
     acgccaaagc gatgggactt aatgtggtag ctgttgatat cagtgatgaa aaatcaaaat       840
     tagccaaaga tttaggtgca gatattgcaa tcaacggttt aaaagaagat cctgtcaaag       900
     cgatacatga tcaagtaggc ggagttcatg ctgctattag tgtagcggtg aataaaaaag       960
     catttgaaca ggcctatcag tcagtgaaac ggggaggcac attagttgtt gtcggtctgc      1020
     caaatgctga tctgcctatt ccgatcttcg atacagtatt aaacggcgtt tccgttaaag      1080
     gttccatcgt cggtacaaga aaggacatgc aggaagcatt agattttgct gctcgcggaa      1140
     aagtccgtcc aattgttgaa accgctgaat tagaagaaat aaatgaagta tttgaaagaa      1200
     tggaaaaagg aaaaatcaac ggtcgtatcg tactaaaatt gaaggaggat tgatgatgag      1260
     ctcaattgca gcccctaaat taaaagaaaa agttgaaaag tttttatccg gcaagaaaaa      1320
     aatgtatata aacggcagct ttgttgaaag cgcttcagga aaaacatttg acactcctaa      1380
     ccctgcaacg ggagaaagat tggcaacagt ttatgaagga gatgctgaag atatcgaccg      1440
     cgctgtgaag gcagccagag aagcgtttga c                                     1471
//
spacer
spacer