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EBI DbfetchID Z23169; SV 1; linear; genomic DNA; STD; PRO; 5157 BP. XX AC Z23169; XX DT 06-JUL-1993 (Rel. 36, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 14) XX DE T.roseopersicina cysJ, cysI, cysH genes, complete CDS, and cysB gene 5' end XX KW alpha subunit of sulfite reductase (NADPH); KW beta subunit sulfite reductase(NADPH); cysB gene; CysB protein; cysH gene; KW cysI gene; cysJ gene; PAPS-reductase; sulfite reductase (NADPH). XX OS Thiocapsa roseopersicina OC Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Chromatiaceae; OC Thiocapsa. XX RN [1] RP 1-5155 RA Schwenn J.D.; RT ; RL Thesis (1994), Fac. Biology, Biochemistry of Plants, Ruhr-University-Bochum XX RN [2] RP 1-5157 RA Haverkamp T., Gisselmann G., Schwenn J.D.; RT "Structure and function of genes involved in the metabolism of sulfite from RT the sulfur oxidizing purple bacterium Thiocapsa roseopersicina"; RL Unpublished. XX RN [3] RC Revised by [4] RA Schwenn J.D.; RT ; RL Submitted (02-JUL-1993) to the EMBL/GenBank/DDBJ databases. RL Schwenn J. D., Ruhr-University-Bochum, Fac. Biology, Biochemistry of RL Plants, Universitaetsstr.150, D 44780 Bochum, Germany XX RN [4] RP 1-5157 RA Schwenn J.D.; RT ; RL Submitted (30-AUG-1996) to the EMBL/GenBank/DDBJ databases. RL Schwenn J. D., Ruhr-University-Bochum, Fac. Biology, Biochemistry of RL Plants, Universitaetsstr.150, D 44780 Bochum, Germany XX RN [5] RP 1-5157 RX PUBMED; 8695637. RA Bruehl A., Haverkamp T., Gisselmann G., Schwenn J.D.; RT "A cDNA clone from Arabidopsis thaliana encoding plastidic RT ferredoxin:sulfite-reductase"; RL Biochim. Biophys. Acta 1295(2):119-124(1996). XX FH Key Location/Qualifiers FH FT source 1..5157 FT /organism="Thiocapsa roseopersicina" FT /strain="6311 (DSM 219)" FT /mol_type="genomic DNA" FT /clone_lib="genomic Sau3A in EMBL 3" FT /clone="pBS subclones pTrJI, pTrIH, pTrBJIH, pTrB" FT /db_xref="taxon:1058" FT CDS 606..2174 FT /transl_table=11 FT /gene="cysJ" FT /standard_name="alpha subunit of hydrogen-sulfide:NADPH+ FT oxidoreductase" FT /product=" sulfite reductase (NADPH)" FT /EC_number="1.8.1.2" FT /note="see also beta subunit of SiR for designation of FT genes as cys genes" FT /db_xref="GOA:P52674" FT /db_xref="HSSP:1DDG" FT /db_xref="InterPro:IPR001433" FT /db_xref="UniProtKB/Swiss-Prot:P52674" FT /protein_id="CAA80687.1" FT /translation="MVLSQLNGLTSPLDERHVHALQIALKGMTPTQLAWVSGYLAGIGG FT GPLRMPTEPKAVERITILFGSQTGNAKAVAEQLGARASEQGMDARVISMGDFNPRKLSK FT EQWVLIVVSTHGEGEPPENAYALHAFIQDQGAGRLEQLPFAVLGLGDSSYEHFCRTAVD FT FDRRLAELGAQRILPLQCCALDYRSDTERWSSDALNRLSALAPRKASNVVAMPTLRSHQ FT DLRSHQEQSRNRARPYDKDNPYTATLLENRRITTLDAVSDVRHLALAIEPDAMHYRPGD FT ALGVWVENDPSLADTILAGVGIDGAARIELGDEELDLRHALIERLELTQLHPTTVRGWS FT RASGRTGAEDAGERLEREEQTRIYLAGNDAFRLPDAGDTPLIMIGAGTGVAPYRAFLQQ FT RAANGHPGRNWLIFGNRHFHRDFLYQLDWQAHRKAGRLDRVSLAFSRDGAEKPYVQQRL FT REEGKEILRWLDAGAHLYVCGATAMGQAVDHALVEIFTIEAGLDPDCASLRRYPARECY FT ATDDL" FT CDS 2178..3857 FT /transl_table=11 FT /gene="cysI" FT /standard_name="beta subunit of hydrogen-sulfide:NADP+ FT oxidoreductase" FT /product=" sulfite reductase(NADPH)" FT /EC_number="1.8.1.2" FT /note="cysI and cysJ refer to homologous genes in E. coli. FT The structural homology of the gene(s) /products in FT Thiocapsa persicina does not prove the same function in FT cysteine biosynthesis." FT /db_xref="GOA:P52673" FT /db_xref="HSSP:7GEP" FT /db_xref="InterPro:IPR005117" FT /db_xref="UniProtKB/Swiss-Prot:P52673" FT /protein_id="CAA80688.1" FT /translation="MSAPIHENERIKARSNCLRGTLRESLADTLTGAISPEDTQISKFH FT GFYQQDHRDRRQARPEQYLEPYFGFMLRAPLPGGVCTPAQWLAIDGMGRELGGGSLRLT FT TRQSFQYHGILKRDIASVIRGINAVMIDSIGGCGDVNRNVLCNPNPVESALHREVYDWA FT KRISEHLLPRTRAYHEIWLDGEQVGGGEDVEPIYGRTYLPRKFKTAVGVPPHNDVDVYA FT NDLGFAAVADGSRLIGFNVSAGGTGRNTGIPATFPRLADVLGFVEPERTLAVAEAVVTT FT QRHFGDRLDRTQARLKYTIERMGLDAFRDEVERRAGIRFAPARPIGFTDQGDRTAWVRG FT QDGRWHLTLYIESGRLIDGPGQSSMQGLREIARIHQGDFRITPNQNLIVARVPEPGKSE FT IEALARKYGLLDKGIALRLNGMSCVALPTCPLAMAEAERYYPDFLAQVERLTRKHGLAE FT QEIVTRMTGCPNGCARPYLAELALVGKGPGRYNLMLGGNGRRYRLNRLYRENLDEAAIL FT AEIDTLLGRYAACRQPGERFGDYLIRDGIVRPVVNPAEDFHE" FT CDS complement(<1..408) FT /transl_table=11 FT /gene="cysB" FT /product="CysB protein" FT /function="translational activator" FT /db_xref="GOA:P52675" FT /db_xref="HSSP:1AL3" FT /db_xref="InterPro:IPR011991" FT /db_xref="UniProtKB/Swiss-Prot:P52675" FT /protein_id="CAA80689.1" FT /translation="MDVRQLRSLVTLVEVRFSVSRAAECLHLVQSAVTQHLKQLEAELG FT TRLFVRHGKRLVGLTEVGEQVLRHACEALRQTGNIVAVGREHLEEERRRIRLGATHTQA FT RYVLPPVIRRFAAAYPAVELQIHQGTPGQLVD" FT RBS complement(414..418) FT RBS 591..595 FT -10_signal 468..473 FT -35_signal 444..449 FT unsure 2144 FT unsure 2902..2926 FT unsure 3027 FT CDS 3850..4569 FT /transl_table=11 FT /gene="cysH" FT /standard_name="phospho-adenylyl sulfate reductase" FT /product="PAPS-reductase" FT /note="designation of gene as cysH only refers to FT structural homology with the gene from E. coli - role in FT cysteine auxotrophy not elucidated. " FT /db_xref="GOA:P52672" FT /db_xref="HSSP:1SUR" FT /db_xref="InterPro:IPR014729" FT /db_xref="UniProtKB/Swiss-Prot:P52672" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA80690.1" FT /translation="MSKPDLDAFLHGDDAALRETNRRLESMPAEDRVRWALEHLPPQHV FT LSSSFGTQSAVMLHLVSRQMPEIPVILVDTGYLFPETYRLVDALTDRFGLNLKVYRPAL FT SPAWQEAGLGRLWEQGADGIERYNRLNKIDPMERALRDLDAGTWFAGLRRQQANSRAEL FT PVLRRQDGRIKFHPIIDWHRPRRARYLRRHDLPDHPLRDQGYVSIGDVHTTVPLLPGML FT EEETRFFGIKRECGLHR" XX SQ Sequence 5157 BP; 958 A; 1732 C; 1632 G; 834 T; 1 other; gtcgacgagt tggccgggtg tgccctggtg gatctgaagc tccaccgccg ggtaggccgc 60 ggcaaaacgc cggatgactg gcggcaggac atagcgggcc tgcgtgtggg tggcgcccag 120 gcgaatacgc cgccgctctt cctccaggtg ctcgcgaccc accgcgacga tattcccggt 180 ctggcgcaag gcctcgcagg cgtggcgcaa gacctgttct cccacctcgg tcaacccgac 240 cagacgtttg ccgtggcgca cgaacagacg tgtgccgagc tccgcctcga gctgtttgag 300 atgctgagtc acggccgact ggacaaggtg cagacactcg gcggcccggc tcacgctgaa 360 gcgaacctcg accagggtca ccagcgaacg taattggcgt acgtccatat cgttgcccgt 420 gataacgacg cataaatggt catttcaaat cgtctcggac ctctgcctac actccttcgc 480 aagaccgaac catgggccaa gccccacggc acggacccgg acccggaccg agccgaccac 540 ccggtccgaa ccgacgcgaa cgctccgaat accaacgcac gacatatgcc aagagatttc 600 gatcgatggt tctttcacag ctaaacggtt tgacgagtcc gctggacgag cgtcacgttc 660 acgccctgca gatcgcattg aaggggatga cgcccacgca gctcgcctgg gtcagcggct 720 accttgcggg catcggaggc ggtcctctac gcatgcccac ggagccgaaa gcggtcgagc 780 gcatcaccat ccttttcggg tcccaaaccg gcaatgccaa agcggtggcc gagcaactcg 840 gagcgcgggc gagcgagcag gggatggacg cccgagtgat ctcgatgggc gacttcaatc 900 cccgaaagct gagcaaggag cagtgggtcc tgatcgtcgt gagcacccac ggcgagggtg 960 agcctccgga gaacgcctat gcactgcatg ccttcatcca ggatcagggc gctgggcggt 1020 tggaacaatt gccgttcgcc gtgctggggc tgggcgactc gagctacgag cacttttgcc 1080 ggaccgccgt agacttcgat cgacgccttg cggagctggg cgcgcagcgg atcctgcccc 1140 tgcaatgctg cgctctggac tatcgatcgg acaccgagcg ctggtcgtcg gacgccttga 1200 accggctgag cgcgctggcc ccgcgaaaag cgagcaacgt cgtggcgatg ccgacgctgc 1260 gctcccatca agacctgcgc tcccatcaag agcagagccg aaaccgggcc cgcccttacg 1320 acaaggacaa cccctacacc gcaaccctgc tcgagaaccg ccgaatcacg accctcgacg 1380 ccgtctcgga cgttcgccat ctggcccttg cgatcgaacc cgacgcgatg cactaccgac 1440 cgggcgatgc gctgggcgtt tgggtcgaga acgatccatc cctcgccgat acgatcttgg 1500 ccggtgtggg gatcgacggc gcggcccgaa ttgaactggg cgacgaggag ctggatctgc 1560 gccatgcact gatcgagcgg ctcgagctga cgcaactgca tcccacgacg gtacgcgggt 1620 ggtcccgagc gagcggacgc accggagctg aagacgctgg cgagcgactc gagcgcgagg 1680 agcagacccg catctacctc gccgggaacg acgccttccg gctgccggac gccggggaca 1740 cgccgttgat catgatcggc gccggcacgg gtgtcgcccc ctatcgcgcc ttcctgcagc 1800 agcgcgccgc caacgggcat cccggccgca actggctgat cttcggcaac cgacacttcc 1860 atcgcgattt tctctatcag ctcgactggc aagcacatcg caaggccgga cgactggatc 1920 gggtcagcct cgccttctca cgcgacgggg ctgaaaaacc ctacgtccag cagcgcctgc 1980 gcgaagaggg caaggagatc ctgcgctggc tcgacgcggg ggcgcatctg tacgtgtgcg 2040 gtgcgaccgc gatgggacag gccgtcgatc acgccctcgt ggagatcttt actatcgagg 2100 ccgggctcga tcccgactgc gcgagcctgc gtcgataccc tgcgcgggag tgctacgcaa 2160 ccgacgacct ttgacgcatg agcgcaccca tccacgagaa cgagcggatc aaggcccgca 2220 gcaactgtct tcgcggtacg cttcgcgaga gtctggccga tacgctgacc ggcgccatca 2280 gcccggaaga cacccagatc agcaagtttc acggcttcta ccaacaggac catcgcgacc 2340 gccgccaagc gcggccggag cagtatctcg agccctattt cggtttcatg ttgcgcgcgc 2400 cgctgccggg cggcgtctgt accccggccc aatggctggc catcgacggc atgggccgcg 2460 agctgggcgg cggcagcctg cgtttgacca cgcgtcagag cttccagtat cacggcatcc 2520 tcaaacgcga catcgcgtcg gtcatccgcg ggatcaacgc cgtcatgatc gactcgatcg 2580 gcggctgcgg cgatgtaaac cgcaacgtgc tctgcaaccc caacccggtc gagtccgccc 2640 tgcatcgcga ggtctacgac tgggccaagc ggatcagcga gcacctgctg ccgcgtaccc 2700 gggcctacca cgagatctgg ctcgacggcg agcaagtcgg cggcggcgag gatgtcgagc 2760 ccatctatgg ccggacctat ctgccacgca agttcaagac cgccgtgggc gtcccgccgc 2820 acaacgatgt cgatgtctat gccaacgacc tgggcttcgc cgctgtcgcc gatgggtcac 2880 gcctgatcgg gttcaacgtc tccgccggcg gcactgggcg caacacgggg ataccggcca 2940 ccttcccgag gctggccgac gtcctcgggt tcgtcgagcc ggagcgcaca cttgcggtcg 3000 ccgaggcggt ggtcacgacc caacgtcatt tcggcgatcg gctcgaccgc acccaggcgc 3060 ggctcaagta caccatcgag cgcatggggc tcgatgcgtt tcgggacgag gtcgagcgtc 3120 gagccgggat ccgtttcgca ccggcacggc ccatcggctt taccgatcaa ggggatcgta 3180 cggcgtgggt caggggccaa gacgggcggt ggcacctcac gctctatatc gagagcggac 3240 gtctgatcga cgggccggga cagagctcca tgcagggtct gcgcgagatc gcccggatcc 3300 accaaggtga ttttcggatc acgccgaacc agaacctgat cgtcgcgcgc gtgcccgagc 3360 ccggaaagtc cgagatcgag gccctggccc gcaagtacgg cctgctggac aagggcatcg 3420 ccctgcggct gaacggcatg tcctgcgtcg cgctgccgac ctgcccgctg gcgatggccg 3480 aggccgagcg ctattacccg gatttcctgg cgcaagtgga gcggttgacc cgcaagcacg 3540 gtctcgcgga acaggagatc gtgacccgaa tgaccggctg ccccaacggc tgcgcaaggc 3600 cgtatctggc cgagctggct ctcgtcggca agggaccggg tcgctacaac ctgatgctcg 3660 gcggcaacgg gcgtcggtac cggctcaacc ggctctaccg cgagaacctc gacgaggcgg 3720 ccattctcgc cgagatcgac accctgctcg gacgctacgc cgcgtgtcgc caacccggcg 3780 agcgcttcgg cgattatctc atccgagacg gtatcgtccg tcccgtcgtg aaccctgcgg 3840 aggacttcca tgagtaagcc cgatctcgac gccttcctcc acggcgacga cgccgccttg 3900 cgagagacca accgccggct ggagtccatg cccgcggagg accgcgtgcg ctgggccctg 3960 gagcatctcc cgccccagca cgtgctctcc tccagcttcg gaacacaatc ggccgtaatg 4020 ctgcacctgg tcagccgcca gatgcccgag atccccgtga ttctggtcga cacgggctat 4080 ctctttcccg agacctatcg actcgtcgac gcgctcacgg accggttcgg cctgaaccta 4140 aaggtctatc gccccgcgct ctccccggcc tggcaggagg cgggcctggg gcgtctgtgg 4200 gagcagggag cggacggcat cgagcgctac aatcggctca acaagatcga cccgatggag 4260 cgcgcgctgc gcgacttgga cgccggcacc tggtttgcgg ggctgcgtcg acaacaggcc 4320 aactcccgcg ccgagctgcc ggtacttcga cgccaggacg gccgcatcaa attccatccg 4380 atcatcgact ggcacaggcc tcgacgtgca cgctacctgc gccgtcatga tctgcccgac 4440 catccgttgc gggatcaagg ctatgtctcg atcggggatg tccacaccac ggtgccgctg 4500 ctgccgggga tgttggagga agagacacgc ttcttcggca tcaagcgcga gtgcggattg 4560 catcgttagt ttcgggcgat ccgcgggaga caccgagaag aaacaccgcc gcgtcgaggc 4620 tttcgtgcca gcgtcggcgg ctagacccga cccggcgtag ggtttggcaa gcagtttgct 4680 gggtttttcg gagtccaggt ccgaggcctt gacgtgacca ggtctccatg gagtcctgac 4740 aagaactgcg gcggcgccgt cgcgaccgat cgagacccga ctccggtcgt ccccagagac 4800 acgtcgtcgc gaaccaaccc cgaggaggaa tgagatggca ccgatacaac ccgaagaggt 4860 tnagagctct ccggcagatc cgctttaccc ggtcggtcac gcgatgtcgt gtcgctcggg 4920 ttcgtgaaga gcatcgcgat ccagggtacg acgatcgagg tggagttcac gccggactcc 4980 atcaacgccg agaaggtcac ggccatggag aacgagaccc gcgcccctct tgcagggact 5040 cgggttcgag acgatccaca tccataccga accgccctac gacgacgatt ccatgctgct 5100 cggcggcgcc tcgatgaacc cgctgcaggt ggatctgaaa gaatacggcc tggatcc 5157 // |
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