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EBI DbfetchID Z22737; SV 1; linear; genomic DNA; STD; PRO; 4245 BP. XX AC Z22737; XX DT 21-MAY-1993 (Rel. 36, Created) DT 06-DEC-1993 (Rel. 38, Last updated, Version 8) XX DE A.eutrophus genes for lactate dehydrogenase, putative membrane-bound DE protein with four times repitition of Pro-Ser-Ala at the N-terminus DE (function unknown) and transglycosidase (partial) XX KW lactate dehydrogenase; transglycosidase. XX OS Cupriavidus necator OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; OC Burkholderiaceae; Cupriavidus. XX RN [1] RP 1-4245 RA Jendrossek D.; RT "The Alcaligenes eutrophus ldh structural gene encodes a novel type of RT lactate dehydrogenase."; RL Unpublished. XX RN [2] RP 1-4245 RA Jendrossek D.; RT ; RL Submitted (12-MAY-1993) to the EMBL/GenBank/DDBJ databases. RL Dieter Jendrossek, Institut fuer Mikrobiologie, Grisebachstrasse 8, RL Goettingen, D-33077, Germany XX RN [3] RX DOI; 10.1016/0378-1097(93)90169-3 RX PUBMED; 8405966. RA Jendrossek D., Kratzin H.D., Steinbuechel A.; RT "The Alcaligenes eutrophus ldh structural gene encodes a novel type of RT lactate dehydrogenase"; RL FEMS Microbiol. Lett. 112(2):229-235(1993). XX DR GOA; P40289. DR InterPro; IPR016130; Tyr_Pase_AS. DR UniProtKB/Swiss-Prot; P40289; Y669_RALEH. XX FH Key Location/Qualifiers FH FT source 1..4245 FT /organism="Cupriavidus necator" FT /strain="H16" FT /mol_type="genomic DNA" FT /db_xref="taxon:106590" FT RBS 947..953 FT CDS 962..2503 FT /transl_table=11 FT /product="putative membrane-bound protein with four times FT repitition of Pro-Ser-Ala at the N-terminus; function FT unknown" FT /db_xref="GOA:Q07252" FT /db_xref="InterPro:IPR001898" FT /db_xref="UniProtKB/Swiss-Prot:Q07252" FT /protein_id="CAA80431.1" FT /translation="MTDTSASPPSAPSAPSAPSAAAAQAVVPAPKLTLHWGLFAAVAAL FT LVVLAIPQPEGLTVAGQRMLAILAFAIVLWITEAVSYETSAIMITSLMAGLIGFAPTVN FT DPSVQYGTSKALGMALAGFSNTALALVAAALFISAAMTVTGLDRRIALVTLSAIGTSTR FT RILIGTIAVTIALSLVVPSATARSACVVPIMMGVIAAFGVDKKSNIAAGIMITVAQATS FT IWNVGIQTAAAQNLLTVGFMDKLLGERITWLQWLIAGAPWAIAMSVVLYFLVRLLLPAE FT TDAIPGGKEAVQRELSALGPMSAPQKRLAAVSLGLLLFWATEGKLHSFDTATVTFVGLV FT ILMMPRIGVMDWKTMQQRTPWGTLIVFGVGISLGTALLSTNAGQWLGQFVVTHSGLAAH FT GALLVFAILSAFLILIHLGFASATALTAALLPILIAVLQTLPGDINRVGMTMLLGFTVS FT FGFILPINAPQNMVCLGTETFNGRQFARIGIPVTIIGYAMMLLFAATYWRWLGWV" FT RBS 2502..2508 FT CDS 2517..3566 FT /transl_table=11 FT /product="lactate dehydrogenase" FT /note="homologies to X51714 (MDH of M. fervidus) and to FT L02123 (E.coli 6018-7103), but no or only little homology FT to other eubacterial or eucaryotic LDHs/MDHs" FT /db_xref="GOA:Q07251" FT /db_xref="InterPro:IPR003767" FT /db_xref="UniProtKB/Swiss-Prot:Q07251" FT /protein_id="CAA80432.1" FT /translation="MKISLTSARQLARDILAAQQVPADIADDVAEHLVESDRCGYISHG FT LSILPNYRTALDGHSVNPQGRAKCVLDQGTLMVFDGDGGFGQHVGKSVMQAAIERVRQH FT GHCIVTLRRSHHLGRMGHYGEMAAAAGFVLLSFTNVINRAPVVAPFGGRVARLTTNPLC FT FAGPMPNGRPPLVVDIATSAIAINKARVLAEKGEPAPEGSIIGADGNPTTDASTMFGEH FT PGALLPFGGHKGYALGVVAELLAGVLSGGGTIQPDNPRGGVATNNLFAVLLNPALDLGL FT DWQSAEVEAFVRYLHDTPPAPGVDRVQYPGEYEAANRAQASDTLNINPAIWRNLERLAQ FT SLNVAVPTA" FT CDS 3625..>4245 FT /transl_table=11 FT /product="putative N-terminus of a transglycosidase" FT /note="homologies to penecillin-binding proteins of E. coli FT and S. pneumoniae and S. oralis" FT /db_xref="GOA:Q07259" FT /db_xref="InterPro:IPR001264" FT /db_xref="UniProtKB/Swiss-Prot:Q07259" FT /protein_id="CAA80433.1" FT /translation="MKRLWSAFIKLLVLAVIGGALLAAIAILAANRQLPSLDALTAFRH FT TPDYVPIGKIPRELTGAVVAIEDERFYVHDGIDYIGVVRAGVANLSDELSQGASTITMQ FT VARNFYLSRDKTYTRKLYEVLLSYRIEKALTKDEILELYMNKIYLGQGAYGFADAARTY FT FGKRLDQLTLAECAMLAGLGKAPSANNPVANPRRARQRQVYILQ" XX SQ Sequence 4245 BP; 663 A; 1505 C; 1362 G; 715 T; 0 other; ctgcagccgc tccaggtccg ccgcgctggc cagcgcgggg ttgccgctgc ggtacaggcg 60 cccctggcgc acgcggcgtc cgcccgcgcc ggccaggccg cccaggtcgc gcgcattgct 120 gatggtgtcg aatgccaggt catgccggcc ctgcacgctg gccgcggccg gagcctgcgg 180 cacgacggca tgggcggaca ggcatcctgc gcgttgaagc cacttgatca ttgttctttc 240 ctgattgtcc ttgcgatgca ggccgatgct gtgccggcgc ttcagcgcgc gcggtacgcc 300 ggcacatgcc agcgcaggtg gcagccggcg atatagcgcc ggtccagccg cgccaccagc 360 ttccacaccg tcagcgcact ggcatcgata tcgacccacg cccacgggtg cttcgggtcg 420 cccgcttcca ccagttccac cgaacggatc ttcgcatacc gcgccagcgc ctcgcgcagt 480 gccgccgcgt ccaggcctgc tttcagtcca cgtactaaaa ccttcatggt cggctccaat 540 tatttcagat cactcccgct catgcatcac ccggccgggg ccatggtggc gcgcttgcgc 600 acacaccgcc gccagacctt atgtggcgag cttctcactg gtatgacgcc tgcggcggcg 660 cgggtatcca ttgatcacac gtggacttga tcttgctcat ggcgggcgga aatagcttgg 720 aatcgccctc gacagggccg gctgccggtc ggaaaattcc tatcatgtcg aacgcggcgg 780 ccacgccagc agcgaggccg ctccgggcac accgcacacg gatgccgtct cgcgaccgac 840 acctgcctgt cctgccggcc gcgctgtgcc agcgccggcg cgccgcactg tctgccgaga 900 gccgtccgag ccaggtgcac ccgggtaccg agcaggatca ccaaccaaaa ggatgccggc 960 aatgacagat acttccgcct cccccccttc cgccccttcc gccccttccg ccccttccgc 1020 cgcggctgca caggctgtcg tgccggcgcc gaaactcacg ctgcactggg gcctgttcgc 1080 cgccgttgct gcgctgctgg tggtgctggc gatcccgcag cccgaggggc tgaccgtcgc 1140 cggccagcgc atgctggcga tcctcgcctt cgccatcgtc ctgtggatca ccgaggcggt 1200 gtcatatgag accagcgcca tcatgatcac ctcgctgatg gcggggctga tcggctttgc 1260 gccgaccgtc aacgacccct cggtgcagta cggcacctcg aaggcgctcg gcatggcgct 1320 ggcggggttc tccaacaccg cgctggcact ggtcgccgcg gcgctgttca tctccgcggc 1380 gatgaccgtg accgggctgg accggcgcat cgcgctggtg acgctgtctg ccattggcac 1440 cagcacgcgc cgcatcctga tcggcacgat cgccgtcacc atcgcgctga gcctggtggt 1500 gcccagcgcc accgcgcgca gtgcctgcgt ggtgccgatc atgatgggcg tgattgcagc 1560 gttcggcgtc gacaagaagt ccaacatcgc cgccggcatc atgatcacgg tggcgcaggc 1620 caccagcatc tggaacgtcg gcatccagac cgccgcggcg cagaacctgc tgacggtggg 1680 cttcatggac aagctgctgg gcgagcgcat cacctggctg caatggctga tcgcgggcgc 1740 gccatgggcc atcgcaatgt cggtggtgct gtatttcctg gtgcgcttgc tgctgcccgc 1800 cgaaaccgac gccatccccg gcggcaagga agccgtgcag cgcgagctgt cggccctcgg 1860 ccccatgagc gcgccgcaga agcggctggc cgcggtatcg ctgggcctgc tgctgttctg 1920 ggccaccgaa ggcaagctgc acagcttcga taccgccacc gtgaccttcg tcggcctggt 1980 gatcctgatg atgccgcgca tcggcgtgat ggactggaag accatgcagc aacgcacgcc 2040 gtggggcacg ctgatcgtgt tcggcgtcgg catcagcctg ggcacggcgc tgctgtccac 2100 caacgccggc cagtggctgg ggcagttcgt ggtcacgcat tccggactgg cggcgcacgg 2160 cgcgctgctg gtgtttgcga tcctgtcggc cttcctgatc ctgatccacc tgggctttgc 2220 cagcgccacc gcgctgaccg cggcactgct gccgatcctg atcgcggtgc tgcagacact 2280 gcccggcgac atcaaccgcg tgggcatgac catgctgctg ggctttaccg tcagcttcgg 2340 ctttatcctg ccgatcaacg cgccgcagaa catggtgtgc ctgggcacgg aaaccttcaa 2400 cggccgccag ttcgcgcgca tcggcatccc cgtgacgatc atcggctacg ccatgatgct 2460 gctgttcgca gccacctact ggcgctggct gggctgggtc tgaggaggta cgaaccatga 2520 agatctccct caccagcgcc cgccagcttg cccgcgacat cctcgccgcg cagcaggtgc 2580 ccgccgacat cgctgacgac gtggccgagc acctggtcga atccgaccgc tgcggctata 2640 tcagccacgg cctgtcgatc ctgcccaact accgcaccgc cctcgacggc cacagcgtca 2700 acccgcaagg ccgcgccaaa tgcgtgctgg accagggcac gctgatggtg ttcgacggcg 2760 acggcggctt cggccagcac gtgggcaagt ccgtgatgca agcagcgatc gagcgcgtgc 2820 gccagcatgg ccactgcatc gtcactctgc gccgctcgca ccatctcggc cgcatgggcc 2880 actacggcga gatggcggcc gccgccggct ttgtgctgct gagcttcacc aacgtgatca 2940 accgcgcgcc ggtggtggcg ccgttcggcg gccgcgtggc gcggctcacc accaacccgc 3000 tgtgtttcgc cggcccgatg cccaacgggc ggccgcctct ggtggtggac atcgccacca 3060 gcgcgattgc catcaacaag gcccgtgtgc tggccgagaa aggcgagccg gcgcccgaag 3120 gcagcatcat cggcgccgac ggcaacccca ccaccgacgc gtcaaccatg ttcggcgaac 3180 accccggcgc gctgctgccc tttggcggcc acaagggcta cgcactgggc gttgtggccg 3240 agctgctggc gggcgtgctg tccggcggcg gtaccatcca gccagacaat ccgcgcggcg 3300 gcgtggccac caacaacctg ttcgcggtgc tgctcaatcc cgcgctggac ctgggcctgg 3360 actggcagag cgccgaggtc gaggcgttcg tgcgctacct gcacgacaca ccgccggcgc 3420 cgggcgtcga ccgcgtgcag taccccggcg agtacgaggc cgccaaccgg gcgcaggcca 3480 gcgacacgct aaacatcaac ccggccatct ggcgcaatct tgagcgcctg gcgcagtcgc 3540 tcaacgtggc cgtccccacg gcctgaaggt tccgcctgcg gcgggactcg caccctgccg 3600 cacacggctg gcacaatacg tgccatgaaa agactctggt ccgccttcat caagctgctg 3660 gtgctcgccg tgatcggcgg cgcgctgctg gctgccatcg ccatcctggc cgccaaccgg 3720 cagctgccgt cgctcgacgc cctcaccgcc ttccgccata cgcccgacta cgtgcccatc 3780 ggcaagatcc cgcgcgagct gaccggcgcc gtggtggcga tcgaggatga gcgcttctac 3840 gtgcatgacg gcatcgacta catcggcgtc gtccgtgccg gcgttgccaa cctgtcggac 3900 gagctgtcgc agggggcatc gaccatcacc atgcaggtgg cacgcaattt ctacctgtcg 3960 cgggacaaga cctatacccg caagctgtac gaggtgctgc tttcataccg gattgaaaag 4020 gcgctgacca aggacgagat cctggagttg tacatgaaca agatctacct gggccagggg 4080 gcgtacggct ttgccgatgc ggcgcggact tactttggca agcggctgga tcagttgacg 4140 ctggctgagt gtgcgatgct tgccgggctg gggaaggcgc cgtcggctaa taatccggtg 4200 gcgaatccga ggcgggcgcg gcagcggcag gtttatatcc tgcag 4245 // |
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