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EBI DbfetchID Z21701; SV 1; linear; genomic DNA; STD; PRO; 3122 BP. XX AC Z21701; XX DT 27-SEP-1993 (Rel. 37, Created) DT 09-SEP-2004 (Rel. 81, Last updated, Version 6) XX DE Lactobacillus delbrueckii pepN gene encoding membrane alanine DE aminopeptidase XX KW membrane alanine aminopeptidase. XX OS Lactobacillus delbrueckii OC Bacteria; Firmicutes; Lactobacillales; Lactobacillaceae; Lactobacillus. XX RN [1] RP 1-3122 RX DOI; 10.1111/j.1432-1033.1993.tb18224.x RX PUBMED; 8223547. RA Klein J.R., Klein U., Schad M., Plapp R.; RT "Cloning, DNA sequence analysis and partial characterization of pepN, a RT lysyl aminopeptidase from Lactobacillus delbruckii ssp. lactis DSM7290"; RL Eur. J. Biochem. 217(1):105-114(1993). XX RN [2] RP 1-3122 RA Klein J.; RT ; RL Submitted (19-FEB-1993) to the EMBL/GenBank/DDBJ databases. RL Klein J., Universitaet Kaiserslautern, Biologie, Erwin-Schroedinger-Strasse RL 23, 6750 Kaiserslautern, Germany XX FH Key Location/Qualifiers FH FT source 1..3122 FT /organism="Lactobacillus delbrueckii" FT /strain="ssp. lactis, DSM7290" FT /mol_type="genomic DNA" FT /db_xref="taxon:1584" FT CDS 316..2847 FT /transl_table=11 FT /gene="pepN" FT /product="membrane alanine aminopeptidase" FT /function="splits amino acids (preferably lys, leu) from FT peptides" FT /EC_number="3.4.11.2" FT /db_xref="GOA:P37896" FT /db_xref="InterPro:IPR001930" FT /db_xref="InterPro:IPR014782" FT /db_xref="UniProtKB/Swiss-Prot:P37896" FT /citation=[1] FT /protein_id="CAA79805.1" FT /translation="MAVKRFYETFHPDHYDLYIDVDRAARSFSGTSTIHGEIQEETVLV FT HQKYMTISKVTVDGKEVPFTFGDDFEGIKIEAGKTGEAVIAIDYSAPLTDTMMGIYPSY FT YQVDGVKKELIGTQFETTFAREAFPCVDEPEAKATFSLALKFDEHEGETVLANMPEDRV FT ENGVHYFKETVRMSSYLVAFAFGEMRSLTTHTKSGVLIGVYSTQAHTEKELTFSLDIAK FT RAIEFYEDFYQTPYPLPQSLQLALPDFSAGAMENWGLVTYREAYLLLDPDNTTLEMKKL FT VATVVTHELAHQWFGDLVTMEWWDNLWLNESFANMMEYLSVDHLEPNWHIWEMFQTSEA FT AAALTRDATDGVQSVHVEVNDPAEIDALFDGAIVYAKGSRMLVMVRSLLGDEALRKGLK FT RYFDKHKFGNAAGDDLWDALSTATDLNIGEIMHTWLDQPGYPVVNAFVEDGHLKLTQKQ FT FFIGEGKEVGRKWEIPLNANFKAPKIMSDVELDLGDYQALRAEAGHALRLNVGNNSHFI FT VKYDQTLMDDIMKEAKDLDPVSQLQLLQDLRLLAEGKQASYADVVPVLELFKNSESHIV FT NDALYTTADKLRQFAPAGSEADKNLRALYNDLSKDQVARLGWLPKAGESDEDIQTRPYV FT LSASLYGRNADSEKQAHEIYVEYADKLAELSADIRPYVLINEVENYGSSELTDKLIGLY FT QATSDPSFKMDLEAAIVKSKDEGELKKIVSWFKNAEIVKPQDLRGWFSGVLSNPAGEQL FT AWDWIRDEWAWLEKTVGGDMEFATFITVISRVFKTKERYDEYNAFFTDKESNMLLNREI FT KMDRKVIANRVDLIASEQADVNAAVAAALQK" FT -10_signal 250..255 FT -35_signal 221..234 FT RBS 305..309 FT terminator 2871..2891 XX SQ Sequence 3122 BP; 861 A; 745 C; 796 G; 720 T; 0 other; gatagggctg aaattatcat tttaagcgct ttaaattagc tatacagata agtaacatta 60 gtaacaattg tcaagagact gcaataaaag gaaaaggcca gctgctagac tggtctttta 120 catatgcaat tatttcaaaa atggaattaa tttccgttag aaactgaatt gtctgatcga 180 gttcaagggg ctgaggtaga ctgcaaacag atattttgcg ttaaatgggc tttatttagc 240 cttttttgct agaatagaga agtgtgaata caatatacgc gaggaaaaat cagacgcgga 300 ttataggagg aaacaatggc tgttaagcgt ttttacgaaa cattccatcc agatcactac 360 gatctataca tcgacgttga ccgggcagca agatctttct cagggacttc caccatccat 420 ggtgaaatcc aggaagaaac tgtcttggtc caccaaaagt acatgaccat cagcaaggtc 480 actgttgacg gcaaggaagt gccgtttact tttggcgacg actttgaagg catcaagatc 540 gaagccggca agactgggga agcggtcatc gcgatcgact actcagcgcc tttgactgac 600 actatgatgg ggatctaccc gtcctactac caagttgatg gcgtcaagaa ggaactgatc 660 gggacccagt ttgaaactac ttttgcccgg gaagccttcc catgtgttga cgaaccagaa 720 gctaaggcaa ccttctccct cgccctcaag tttgacgaac acgaaggcga aactgttttg 780 gccaacatgc cagaagaccg ggtagaaaac ggcgtgcact actttaagga gactgtccgc 840 atgtccagct acctggttgc ttttgccttt ggtgaaatgc ggtcattgac cacccacacc 900 aagagcgggg tcttgatcgg ggtttactca actcaagccc acactgaaaa ggaactgacc 960 ttctccttgg acattgccaa gcgggcaatt gaattctacg aagactttta ccaaactcca 1020 tatccgctgc cgcaatcact gcagctggcc ctgcctgact tctcagccgg tgccatggaa 1080 aactggggtc tggtaaccta ccgggaagcc tacctgcttt tggacccgga caacaccact 1140 ttggaaatga agaagctggt tgcgactgtg gtgacccacg aactggccca ccaatggttc 1200 ggtgacctgg taaccatgga atggtgggac aacctctggc tgaacgaaag tttcgccaac 1260 atgatggaat acctgtcagt tgaccacctg gaacctaact ggcacatctg ggaaatgttc 1320 cagacttctg aagcagcggc tgccttgacc cgggatgcaa ccgacggggt acagtcagtg 1380 cacgtggaag ttaatgaccc ggctgaaatc gacgccctct ttgacggggc catcgtttac 1440 gccaaggggt caagaatgct ggtcatggtc cggtcacttt tgggcgatga agccttgaga 1500 aagggcttga agcgctactt tgacaagcac aagtttggca acgcggcagg tgacgatctc 1560 tgggatgcct tgtcaacggc cactgacttg aacattgggg aaatcatgca cacttggctg 1620 gaccagccag gctacccagt ggtgaatgct tttgttgagg acggccactt gaagctgact 1680 cagaagcaat tcttcatcgg tgaaggcaag gaagtcggcc gcaagtggga aattccgctt 1740 aacgctaact tcaaggcacc gaagatcatg tcagacgttg aacttgacct gggtgactac 1800 caggctctgc gggcagaagc cggccacgct ctgcgcttga acgtgggcaa caactcccac 1860 ttcatcgtga agtacgacca gactttgatg gacgacatca tgaaggaagc caaggacttg 1920 gatccagttt cccaattgca attgctgcaa gacctgcggc ttttggcaga aggcaagcag 1980 gcttcatacg ctgacgtggt accagttctg gaactcttca agaactcaga aagccacatt 2040 gtcaacgatg ctctgtacac gactgctgat aagctgcggc aatttgcccc agccggcagt 2100 gaagctgaca agaacctgcg ggctctgtac aacgacttgt ccaaggacca agttgcccgt 2160 ttgggctggc tgcctaaggc aggggaaagc gatgaagaca ttcagacccg gccatacgtt 2220 ttgtctgcta gcctttacgg ccgcaacgct gattcagaaa agcaagccca cgaaatctac 2280 gtggaatacg ctgataagtt ggcagaactg tccgctgata tccggccata cgttttgatc 2340 aacgaagttg aaaactacgg gtcaagcgaa ttgactgaca agctgattgg tttgtaccag 2400 gcaaccagtg acccatcatt caagatggac ctggaagccg cgatcgtgaa gagcaaggac 2460 gaaggcgaac tgaagaagat cgtttcctgg ttcaaaaacg ctgaaatcgt taagccgcag 2520 gacttgcgcg gctggttcag cggcgttttg tccaacccgg caggtgaaca gctggcctgg 2580 gactggatca gagacgaatg ggcatggttg gaaaagacgg tcggcggcga catggaattc 2640 gctaccttca tcactgtcat ctcccgcgtc ttcaagacca aggaacgcta cgacgaatac 2700 aacgccttct ttactgacaa ggaaagcaac atgctgctga accgggaaat caagatggac 2760 cggaaggtca tcgctaaccg ggtagacttg attgccagcg aacaagctga cgtcaacgcc 2820 gcggttgctg ctgctttgca aaagtaattg aatagagcat aagaaaactg tttccgctga 2880 gagctggaaa cagttttttt atgtattcaa cttgtctgca atcggttaca atatagatgt 2940 aaatactatc gtacattctt tgaggtaata aaatgaacaa cgattttaaa gatatcatgc 3000 aaaacagaaa gtctatccgg cactatgatt ccagcgtgaa gatttcccgt gacgaattgc 3060 tggaaatcat taatgaatct atctctgctc caagtgcctg caacctgcag tcctggaagc 3120 tt 3122 // |
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