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EBI DbfetchID Z19598; SV 1; linear; genomic DNA; STD; PRO; 6449 BP. XX AC Z19598; XX DT 28-JUN-1995 (Rel. 44, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 18) XX DE P.laminosum nrtA-Phl, nir-Phl, nrtB-Phl and nrtC-Phl genes XX KW ATP binding protein; nir-Phl gene; nitrite reductase; nrtA-Phl gene; KW nrtB-Phl gene; nrtC-Phl gene; periplasmic protein. XX OS Phormidium laminosum OC Bacteria; Cyanobacteria; Oscillatoriales; Phormidium. XX RN [1] RP 1-5201 RA Merchan F.; RT ; RL Thesis (1993), Biochemistry and Molecular Biology, University of Basque RL Country XX RN [2] RC Revised by [4] RA Merchan F.; RT ; RL Submitted (15-JAN-1993) to the EMBL/GenBank/DDBJ databases. RL Merchan F., University of Basque Country, Bilbao, Vizcaya, SPAIN. XX RN [3] RP 1-5201 RX DOI; 10.1007/BF00037030 RX PUBMED; 7766873. RA Merchan F., Kindle K.L., Llama M.J., Serra J.L., Fernandez E.; RT "Isolation, sequence and expression in Escherichia coli of the nitrite RT reductase gene from the filamentous, thermophilic cyanobacterium Phormidium RT laminosum"; RL Plant Mol. Biol. 27(5):1037-1042(1995). XX RN [4] RP 1-6449 RA Fernandez E.; RT ; RL Submitted (12-JAN-1996) to the EMBL/GenBank/DDBJ databases. RL E.Fernandez, Biochemistry and Molecular Biology, University of Cordoba, RL 14071-Cordoba, Spain, e-mail bb1feree@seneca.uco.es XX RN [5] RP 1-6449 RX DOI; 10.1007/BF00021199 RX PUBMED; 7647306. RA Merchan F., Kindle K.L., Llama M.J., Serra J.L., Fernandez E.; RT "Cloning and sequencing of the nitrate transport system from the RT thermophilic, filamentous cyanobacterium Phormidium laminosum: comparative RT analysis with the homologous system from Synechococcus sp. PCC 7942"; RL Plant Mol. Biol. 28(4):759-766(1995). XX FH Key Location/Qualifiers FH FT source 1..6449 FT /organism="Phormidium laminosum" FT /strain="OH-1-p-Cl1" FT /mol_type="genomic DNA" FT /db_xref="taxon:32059" FT CDS 181..1713 FT /transl_table=11 FT /gene="nir-Phl" FT /product="nitrite reductase" FT /function="reduction of nitrite to ammonium" FT /db_xref="GOA:Q51879" FT /db_xref="InterPro:IPR005117" FT /db_xref="UniProtKB/Swiss-Prot:Q51879" FT /citation=[1] FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA79655.1" FT /translation="MTSTVPAETSLNKFEKLKSEKDGLAVKSELEDFARLGWEAMDETD FT RDHRLRWMGVFFRPVSQGKFMLRMRIPNGILTSGQIRVLAEVVERYGEDGNADITTRPN FT LQLRGIRLEDIPDIFRRFEQAGLTSIQSGMDNVRNITGSPVAGIDADELIDTRGLVRKV FT QDMITNNGEGNPSFSNLPRKFNIAIAGCRDNSVHAEINDIAFVPAYKDGKLGFNVLVGG FT FFSAKRCEAAVPLNAWVDPRDVVALCEAILIVYRITGCGANRQKSRLMWLIDEWGMDKF FT RAEVEQQLGHPLQTAAPKDEILWDKRDHIGIHAQKKPGLNYVGLLVPVGRLYAPMFDLA FT RIAEVYGDGEMRLTVERKRDHSRTCPMSSVASLLKEPLLEKFSVSPGLLVRSLVSCTGA FT QFCNFALIETKNRAMALIRELESELELARPVRIHWTGCPNSCGQPQVADIGLMGTKVRK FT DGKATEGVDLYMGGKVGKHAELGTCVQKGIPCDDLKPILRNLLIEHFGARPK" FT RBS 1964..1969 FT CDS 1978..3306 FT /transl_table=11 FT /gene="nrtA-Phl" FT /standard_name="NrtA" FT /product="probable periplasmic protein" FT /function="probably binds specifically nitrate" FT /db_xref="InterPro:IPR017909" FT /db_xref="UniProtKB/TrEMBL:Q51880" FT /citation=[1] FT /protein_id="CAA79656.1" FT /translation="MSSLSRRKFIFTAGVTAGATILANGCSGGSSPSDTSSPAASAPLA FT PAPVAAADTPEVTTAKLGFICPDGLAPLIIAKEKGLFAKYGMPDVEVLKQASWAVTRDN FT LELGSGGGGIDGAHILTPMPYLMSAGTITKGNQKVPMNILARLNVNGQGICLANTYKDL FT KIGTDSSPLKEAFAKAKAEKGEIKAAVTFPGGTHDLWMRYWLSAGGIDPDKDISTIVVP FT PPQMVANIKVNNMETFCVGEPWPAQTVNQGLGYNAMTTGELWKDHPEKAFAMRADWVEQ FT HPKAAKALLMAVQEAQIWCDDPANKEEMCQIVSGREWFKVPVEDILERSKGNFDLGVRQ FT LENSPLLMKFWNDAASYPFKSHDLWFLTEDIRWGYLPADTDTKALVDAVNREDLWREAA FT KAIGQEAAIPASTSRGVETFFDGVKFDPENPSAYLSALKLKKV" FT RBS 3309..3313 FT CDS 3478..4317 FT /transl_table=11 FT /gene="nrtB-Phl" FT /standard_name="hydrophobic protein" FT /product="hydrophobic protein" FT /function="probably traslocates nitrate through the FT membrane" FT /db_xref="GOA:Q51881" FT /db_xref="InterPro:IPR005889" FT /db_xref="UniProtKB/Swiss-Prot:Q51881" FT /citation=[1] FT /protein_id="CAA79657.1" FT /translation="MTADLSSPRRARRSPSSYLGALVGKTIRRSIPTAIAFFVLLGIWQ FT LLCSGENAACRPIQVVQESWDLIIDPFYRGSGTDQGLFWQIAASLQRVAVGYLMAAIAG FT IALGILIGVSVLMFQALDPIFQVLRTVPPLAWLPISLAAFRDSQPAAIFVIFITAIWPI FT IINTAVGVQNIPQDYNNVARVLKLSRLDYFLKVLLPATVPYIFTGLKIAIGLSWLAIVA FT AEMLTGGVGIGFFIWDAYNSGSNSEVILAIIYVGLVGLLLNALVGFIASRVVPDEQK" FT RBS 4316..4320 FT CDS 4432..6309 FT /transl_table=11 FT /gene="nrtC-Phl" FT /product="ATP binding protein" FT /function="Binds and probably hydrolizes ATP" FT /db_xref="GOA:Q51882" FT /db_xref="HSSP:1F3O" FT /db_xref="InterPro:IPR005890" FT /db_xref="UniProtKB/TrEMBL:Q51882" FT /citation=[1] FT /protein_id="CAA79658.1" FT /translation="MLPFVEIDHVDQVFPLPGGGEYIALKNIDLTIRKGEFVSLLGHSG FT CGKSTLLNIIAGLAKPAAGALILEGRQVTEPGPDRMVVFQNYSLLPWLTVRENIALAVN FT RVMATASKSEREAIVEQHIDLVNLRHAADKRPGQLSGGMKQRVAIARALSIRPKLLLLD FT EPFGALDALTRSSLQDQLMQIAEEHELTCIMVTHDVDEALLLSDRIVMLTNGPESYIGQ FT IMDVPFERPRDRLEVVNDPSYYGMRSEIVYFLNQQKQAKKRKAALQVGAIARNGLEKVN FT LELGFVPLTDCAPLVVAKELGFFEKYGLEQVTLSREPSWKAIAEDITSGRLDAAQMIAG FT MPLALTLGMGGKALYPSPQASSSAATATRLRSSKRFLHDGVRTVEDLKAAIARTPDKVP FT TMAVVHPASMHNLMTRHWLASGGIDPDRDVNIVTIPPPQMVAALKGGGIDGFCVGEPWN FT SRAIQEGLGYAIAPCAQPVRHDDQRRGCGHFRQIQVNRERAIFLRRASANALGIHQPQR FT LLLEGDGTGWRGSMGGRVCSRSDSVWANRLRRIDHPICHRGRSLQQPAQPPLKSDRLPD FT IQLHIHWQMSQKAQHAEAVWVGRRQNARTPQAAGTVSPRSRPQQPAHLF" XX SQ Sequence 6449 BP; 1396 A; 1861 C; 1849 G; 1343 T; 0 other; tcactcatac gaaacccttt ccctccagat agcgacctgt tcgtcaagtc tcctgagata 60 aaactttaga agtctgggcg actgtatcta aaagcaagtg gtttcgggat ttgcttcgca 120 atctgttctg aactttgttc cgaagcccgt tcacgtttct ctcatcaaag gtcttttgca 180 atgaccagta ccgttccagc agaaaccagt ctgaataagt ttgaaaagct caagtccgaa 240 aaagacggtc tggcggttaa gtctgaactg gaagactttg cccgtctggg ctgggaagcg 300 atggatgaaa ccgaccggga tcatcgcctg aggtggatgg gcgtgttttt tcgcccggta 360 agccagggta agtttatgct acggatgcgg attcccaatg gcattctcac cagtggccaa 420 attcgggttc tggcagaggt ggtcgagcgc tacggcgaag acggcaacgc cgacatcacc 480 acgcgcccaa acctccagct tcgcggcatt cggctagaag atattcccga catctttcga 540 cggttcgagc aggcggggct gaccagcatc cagtccggca tggacaatgt tcgcaacatc 600 acgggttcgc ccgtggcagg catcgatgcc gacgagctaa tcgacactcg cggactggtt 660 cgcaaggtgc aagacatgat caccaacaac ggcgagggca acccgtcgtt tagtaatctg 720 ccgcgcaagt tcaacattgc gatcgccgga tgccgcgata actcggttca cgccgagatc 780 aacgacatcg cctttgtgcc cgcctacaaa gacggcaagc tgggctttaa cgtactcgtc 840 ggcggattct tttcggccaa gcgctgcgaa gccgccgtgc cgctcaatgc ctgggtcgat 900 ccgcgagacg tggtggcgct gtgcgaagcc atcctgatcg tctatcgaat cacgggctgc 960 ggggccaacc gccaaaagtc tcgcctgatg tggctaatcg acgagtgggg tatggataag 1020 tttcgcgcag aggtggagca gcagttgggt cacccgctgc aaaccgccgc gcccaaagat 1080 gaaatcctct gggacaagcg cgaccacatc ggcatccacg cccaaaagaa gccagggctg 1140 aattacgtag ggctgctagt gcccgtcggt cggctctatg caccgatgtt tgacctagct 1200 cgcattgccg aagtctacgg cgacggcgaa atgcgtctga cggtcgaacg aaaacgtgat 1260 cattcccgca cgtgcccgat gtcctccgtt gcgtccttac tcaaagagcc gctgctagag 1320 aagttttcgg tgagtcccgg cttattagtg cgatcgctcg tttcctgcac cggggcccag 1380 ttctgcaatt tcgccctgat cgaaaccaaa aatcgagcga tggcgctgat tcgagaactg 1440 gaatccgagc tggaactcgc tcgccccgtc cgcatccact ggacgggctg ccccaactcc 1500 tgcggccagc cccaagttgc cgacattggt ctgatgggca ccaaagtccg caaagacggt 1560 aaagctaccg aaggcgtgga tctgtacatg ggcggcaaag tcggcaaaca cgccgaactc 1620 ggcacctgtg tccaaaaagg aattccctgc gatgacctca aacccatcct gcggaatctg 1680 ctgatcgaac actttggcgc aagaccgaaa tgaggggtta ggggtcaggt gtcaggggtt 1740 agggatgagc ctggccccgc tcctagtctt caaacttgaa tcaatcaggc tgaggaatca 1800 aagctgaatg agatttaagt gcgaacctgc ctgcacgcct aacccctaac ccctaacccc 1860 caaccctagc caagccagtt tctcaccgga tcttgacacc gggcgcaact ggtaggggat 1920 tttacatcca aaattggtca actgattacc ctaacctcaa cttggagaac gcaacctatg 1980 tctagtctct ctcgtcgcaa attcatcttc acggctggag tcaccgctgg ggcaacgatc 2040 ctagcgaatg gatgcagcgg tggctcttcg ccctcagata ccagcagccc cgccgccagc 2100 gctcccctgg cgcccgcacc agtggccgca gccgacacgc ccgaagtcac gactgccaag 2160 ctgggtttca tttgccctga cggactcgct ccactgatta ttgccaagga aaaaggccta 2220 tttgccaagt atggaatgcc cgacgtggaa gtgctaaagc aggcatcctg ggccgtcacc 2280 cgcgacaacc tagaactggg atcgggcggc ggcggcatcg atggggcaca catccttacg 2340 cccatgcctt atctgatgtc ggcaggtacg atcaccaagg gcaaccagaa agtgccgatg 2400 aatattctgg caaggctgaa tgtcaacggt caggggattt gtttggcaaa cacctacaag 2460 gatctaaaga ttgggacaga cagttcgccg ctgaaggaag cgtttgccaa ggcaaaagca 2520 gagaagggcg aaatcaaagc agccgtgacg tttcccggtg ggacgcacga cctgtggatg 2580 cgctattggc tgtcagcggg cggtattgat cccgacaagg atatttcgac catcgtggtg 2640 ccgccgcccc agatggtggc caacatcaag gtcaacaaca tggagacttt ttgtgtgggc 2700 gaaccctggc ccgcccaaac ggtgaaccaa ggtctcggct ataacgcaat gaccacgggt 2760 gaactttgga aagaccatcc tgaaaaggca tttgcgatgc gggccgactg ggtagagcaa 2820 catcccaagg cagcaaaggc gctgctaatg gcagtgcaag aggcccaaat ctggtgtgat 2880 gaccctgcca acaaggaaga aatgtgccag attgtatcgg gtcgcgagtg gttcaaggtt 2940 ccagtagagg acattctaga acgctcgaag ggtaattttg acctgggtgt gcgccagctt 3000 gaaaatagcc cgctgctgat gaagttttgg aatgatgcgg catcttatcc gttcaaaagc 3060 catgacctgt ggttcctgac agaagatatc cgctggggct atttgcctgc cgacaccgac 3120 accaaagcac tggtggacgc ggtgaaccgg gaagacctct ggcgcgaagc ggcgaaggcg 3180 atcggtcaag aagccgccat tcccgccagc acctcgcgtg gtgtggaaac cttctttgat 3240 ggagtcaagt ttgatcccga aaaccccagt gcttatctca gcgctctgaa gctcaagaaa 3300 gtctgagcag gaatcagggg ttaggggtta ggggttaggg gttaggtcgc aagccccgct 3360 tcgagatatg caaagtttgc ttactttgtc ttatcctact tgactcctaa tccctaattc 3420 ccaatcccta attcccaatc cctaattccc caacccctaa ttccccaact cctaatcatg 3480 acagccgacc tctcttctcc tcgccgcgct cggcgatcgc cctcttctta cttgggtgcg 3540 ctagttggta agacgattcg ccgctcaatt ccaacggcga tcgccttctt cgttctcctg 3600 ggcatctggc aactcctgtg ttcgggcgag aatgccgcct gcaggccgat ccaggtggtg 3660 caagaatcgt gggatctgat catcgacccg ttctatcgcg gtagcggcac cgatcagggc 3720 ctgttttggc agattgccgc gagcttgcag cgggtggcgg tgggttatct aatggcggcg 3780 atcgccggaa ttgcgctggg tatcttaatc ggcgtgagtg tgctcatgtt tcaggcgctc 3840 gacccgatct ttcaggtgtt gcgaaccgtg ccgccgctgg cctggctgcc catctccctg 3900 gccgccttcc gcgactctca gcccgctgcc attttcgtaa ttttcatcac cgccatctgg 3960 cccatcatca tcaacaccgc agttggtgta caaaacattc cccaggacta caacaacgtc 4020 gcccgcgtgc tgaagttgtc gcggctcgat tacttcctga aggtgctgct gcccgccact 4080 gtgccctaca tcttcacggg actcaagatt gcgattggct tatcctggct ggcgatcgtt 4140 gctgccgaaa tgctcacagg tggcgttggc atcggcttct ttatctggga tgcctacaac 4200 agcggtagca atagcgaagt gattctcgcc atcatctacg ttggtctagt cggtcttttg 4260 ctgaacgcgc tagtcggctt cattgcctcc cgtgtcgtac ccgacgaaca gaagtaggaa 4320 tcaggggtta ggggttaggg agggctttcc tttacttact ccagcatccg tcaccccaac 4380 acccttgccc tgtcactaca gcaccaattc atccttcatc cctcatccct catgctgcca 4440 tttgtagaaa ttgatcatgt agatcaggtg ttcccgctac cgggcggcgg cgaatacatt 4500 gcgctgaaga acattgacct gacgattcgc aagggcgagt ttgtctcgct gctgggtcat 4560 tcgggctgcg gcaaatcgac gctgctgaac atcattgcag ggttggcaaa accagcggcg 4620 ggcgcgttga ttttggaagg tcgtcaggtg acggagccgg gccccgaccg catggtggtg 4680 tttcaaaatt actcgctgct gccctggctg acggtgcgcg aaaacattgc cctggcggtg 4740 aaccgcgtca tggcgaccgc ctccaagtcg gagcgggaag cgattgtaga acagcatatc 4800 gacttagtga acctgcgcca cgcggcagac aagcgaccgg ggcagctctc tggcggcatg 4860 aagcagcgag tggcgatcgc ccgtgccctc tccattcgcc ctaaactctt gctgctggat 4920 gaaccctttg gggcgctaga tgccctcacc cgcagcagct tgcaagacca gttgatgcaa 4980 attgccgagg aacatgaact cacctgcatc atggtgacgc acgatgtgga cgaggcgctg 5040 ctgctgagcg atcgcattgt aatgctgacc aacgggccgg aatcctacat cgggcaaatt 5100 atggacgtgc cctttgagcg gccgcgcgat cgcctagagg tggtgaacga tcccagctat 5160 tacgggatgc ggagcgagat tgtctacttc ctgaatcagc aaaagcaggc gaagaagcgc 5220 aaggcggcgt tgcaggtggg ggcgatcgcc cgcaatggtc tggagaaggt gaacctggag 5280 ttgggctttg tgccgctgac ggactgtgcg ccgctggtgg tagccaagga actgggcttt 5340 tttgagaaat atgggctaga gcaggtgacg ctcagccgcg aaccaagctg gaaggcgatc 5400 gccgaagata ttaccagcgg ccgcctcgat gccgcgcaaa tgatcgccgg aatgcccctg 5460 gcgctgacgc tgggcatggg cggcaaagcc ctgtacccgt caccacaggc ctcgtcctca 5520 gccgcaacgg caacgcgatt acgatcaagc aagcgctttt tgcacgacgg cgtgcggacg 5580 gtggaagatt tgaaagcggc gatcgcccgc acgcccgaca aagtgcccac gatggcggtc 5640 gttcaccccg cttcgatgca caacctgatg acgcgccact ggcttgcctc cggcggcatc 5700 gaccccgata gggatgtaaa catcgtcacc atcccaccgc cgcaaatggt ggctgccctc 5760 aaaggcggcg gcattgacgg cttctgcgtg ggcgaaccgt ggaactcccg cgccattcag 5820 gaagggctgg gctatgcgat cgcgccgtgc gcccagcctg ttcgacacga cgatcagcgg 5880 cgcgggtgtg gacacttccg gcagatacag gtcaaccgtg agcgggcgat ttttcttcgc 5940 agggctagtg ccaacgcgct gggcatccac cagccgcagc gtttgttgtt ggaaggggac 6000 ggaaccgggt ggcgcgggtc gatgggcggc agggtttgca gcaggtcgga ttccgtttgg 6060 gcgaatcgcc tgcgtcgtat cgatcacccg atttgtcacc gaggccgttc gttgcagcag 6120 cccgcccagc cgccgctgaa aagcgatcgc ctgcccgaca tccagctgca tatccactgg 6180 caaatgtcgc agaaagctca gcacgctgaa gccgtctggg tcggccgccg ccaaaatgcc 6240 cgcactccgc aggctgctgg caccgtttcg cccagaagcc gtccgcagca gcctgcccac 6300 ctcttctagc agccgttccc ccgaccgcgt gtagctaaac tgcgacacca tcatcaagtc 6360 cacgtcgtag gtggcgttga gggtgttttg aagctgcgat cgctgatggg gcgcaagccg 6420 ccggagatag aagcggaggt gggggatcc 6449 // |
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