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EBI DbfetchID Z14112; SV 1; linear; genomic DNA; STD; PRO; 2824 BP. XX AC Z14112; XX DT 24-JUL-1992 (Rel. 32, Created) DT 09-SEP-2004 (Rel. 81, Last updated, Version 3) XX DE B.firmus TopA gene encoding DNA topoisomerase I. XX KW DNA topoisomerase I. XX OS Bacillus firmus OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RC (sites) RX DOI; 10.1093/nar/20.18.4928 RX PUBMED; 1329032. RA Ivey D.Mack., Cheng J., Krulwich T.A.; RT "A 1.6 kb region of Bacillus firmus OF4 DNA encodes a homolog of RT Escherichia coli and yeast DNA topoisomerases and may contain a RT translational readthrough of UGA"; RL Nucleic Acids Res. 20(18):4928-4928(1992). XX RN [2] RP 1-2824 RA Ivey D.M.; RT ; RL Submitted (23-JUL-1992) to the EMBL/GenBank/DDBJ databases. RL Ivey D. M., Mount Sinai School of Medicine, Biochemistry, 1 Gustave L. Levy RL Place, New York, NY, USA, 10029 XX FH Key Location/Qualifiers FH FT source 1..2824 FT /organism="Bacillus firmus" FT /strain="OF4" FT /mol_type="genomic DNA" FT /clone_lib="pGEM3/MboI" FT /clone="pJB20" FT /db_xref="taxon:1399" FT CDS <1..790 FT /codon_start=2 FT /transl_table=11 FT /gene="TopA" FT /product="DNA topoisomerase I" FT /function="Catalyzes the relaxation of negatively FT supercoiled DNA" FT /db_xref="GOA:P34184" FT /db_xref="InterPro:IPR003602" FT /db_xref="UniProtKB/Swiss-Prot:P34184" FT /protein_id="CAA78484.1" FT /translation="IYVAPPSSLLPEGALICAAVGHILEFLEPGELNEKYKSYSLDSLP FT IIIDLFQYKVVSDKKEVLQRIKDTIFDKRVKTIILATDAAAEGEYIGRNILYRLHCKKT FT IKRLWTSSMTAFSIQKAFSQLKADAETLPLYYQAKARAESDYMIGLTLSRAYGILLKEQ FT GIVPHNTTISLGRVQTPLLAEIVKRERLLEQFTAENFWTVKATFNNQGNVYEGEWFHEK FT ENRIFTEEQDEQLCELVRNQSSTIMEMKEEMRTYQPPLLY" FT CDS 806..1522 FT /transl_table=11 FT /gene="TopA" FT /product="DNA topoisomerase I" FT /function="Catalyzes the relaxation of negatively FT supercoiled DNA" FT /db_xref="GOA:P34184" FT /db_xref="InterPro:IPR003602" FT /db_xref="UniProtKB/Swiss-Prot:P34184" FT /citation=[1] FT /protein_id="CAA78485.1" FT /translation="MDAGNAFGFKPAETLKYAQSLYDKGYLSYPRTQDERITESDAREL FT ENNIQFLSGHDTSGALFPLPVSTLMNNKRYIGEVTDHHALLITDKIPKDKDLSEDEKSI FT YHLVVKRILAAHYPDVAMSHKEIITKVMDRFTFRSKGKELLSKGRHHIIPPTNENDIML FT PTLLKGSEGVVTDTLTTKSKTKPPNRYTSSSLIGFMKNAAQAIEDEDRKSISNLPLGTE FT ATRAGLTHLVRIEKIY" XX SQ Sequence 2824 BP; 1021 A; 478 C; 544 G; 781 T; 0 other; gatctatgta gcccctccgt cctctttact acctgaagga gcattaatct gcgctgctgt 60 agggcatata ttagaatttc ttgaaccggg tgagttgaat gagaaatata agtcctattc 120 attagatagt ttgccgataa tcatagacct gtttcaatac aaggtagttt ctgataaaaa 180 agaggtctta caaaggataa aagacaccat ctttgataag cgtgtgaaaa ccatcatttt 240 agcaacagat gctgcggctg aaggcgagta cataggaaga aatattctct atcgccttca 300 ctgtaagaaa acaataaaaa gactttggac ttctagtatg acagcatttt ctattcaaaa 360 agctttcagc caattgaaag cagatgctga aacattacca ctatattatc aggcaaaggc 420 cagagctgag tctgattata tgattggact gaccctatct agagcctatg gcatcctatt 480 aaaggagcaa ggaattgtcc ctcataacac aacgatcagc ttagggaggg tgcaaacgcc 540 tttattagca gagatcgtta aaagagagcg gctacttgaa caatttacag cagaaaattt 600 ttggacagta aaagcaacct ttaataatca agggaatgtt tatgaagggg aatggttcca 660 cgaaaaggaa aaccgaattt ttacagaaga acaagatgag caattatgcg agttagttcg 720 caatcagtca agtacgatta tggaaatgaa agaagaaatg cgaacgtatc agcccccctt 780 attatattga ttatctactt tacaaatgga cgctggtaat gcttttggct ttaaacctgc 840 agaaacatta aaatatgctc aatcccttta cgataaagga tatttgagct accctcgtac 900 acaagatgag cgcataactg aaagtgatgc tcgtgaatta gaaaataata tacaatttct 960 aagcggccat gatacatctg gtgcactatt tcctctccct gtaagcactc ttatgaacaa 1020 taaacgttat ataggggaag taaccgatca ccacgctctt cttattacag ataaaattcc 1080 taaagataag gatctatcag aggatgaaaa gtctatctat catttagttg taaaacgaat 1140 ccttgcagct cattatcctg atgtcgctat gtcccataaa gaaataataa caaaggtgat 1200 ggaccgcttt acatttagaa gtaaagggaa agaattgctt tccaaaggtc ggcaccatat 1260 tattcctcct acaaatgaaa atgatatcat gttacctaca cttttaaagg gaagcgaagg 1320 tgttgttaca gacactctca ctactaagtc taagactaag ccaccaaatc gctacacctc 1380 ttcgagtcta attggattta tgaagaatgc tgcacaggcc atagaagatg aagataggaa 1440 atcgattagc aacttacctt tgggtactga ggcaactcga gcggggctaa ctcacctagt 1500 tagaatcgag aaaatatatt gaatggaaga aaaataaagt ttatccaacc ttgctaggta 1560 taacggccgt tgattctatt aaaaggggat cagtaattaa aagccccatt ctaactgcta 1620 aatgggacgt gaaacttaat gaaattggcg cttcccttta taatcacaaa gattttattg 1680 ctcattcgaa aaagctaagc tcggtattgt ttgaagaggt aaaaacctat tcctctacat 1740 ggaatcagaa cggtgttgaa cgtattaaga gcgaaagtat tggagcctgt cttctgtgtg 1800 gcagtaatgt ggtattgaga aaaggaaaac atggtgaatt ttacggatgt tctaattata 1860 aggattcagg ttgtacattt aacttaccat tcaaagtgtt aaataaaaag ttatcaaaaa 1920 agcaacttat ggaacttctt aagaatgaaa aaacagatat aataaaaggg ttcaagtgga 1980 aggataaaac gtttaatgca cctcttgtct ggaatagaga ggatcaaaaa gtccaatttg 2040 gtaaataaac gaaattttat agtgaatttg ttctataatt tattatagta tatactttat 2100 ttatcaattt ttactaggag gtcctactat gattgacttt tctaaatacc ctacgacatt 2160 aaatctaatt aaccaagtag aggacttaaa agatcaacct gaaaaaataa aatggttaca 2220 tttatctaaa gaggaaatcg cgacagccat tgcaaaacaa tatattttat ctgagtgtct 2280 tcggtcaact catcttacat ttagaagaaa agctaatcac tggaacaagg cagtgcatga 2340 aaatgaatct tcaaaagtac attcattcgc tagaactgaa gcgatggatc aattacaact 2400 ttcctatgag gatgttgttc gtttatttcc cgaaagtgaa cttggcggga aaaagcacat 2460 aaactagcgg aaacaagggg ggaaagttta tcactgacaa gacaacttgt tggcggatga 2520 cgtcatcatg aacaactatt taacgggaga taaatacgat attacagaaa gaaagaagat 2580 gagcttgaaa gagtcgccca aggcatatgt tcagtcacca aaatgagtaa tattgaatta 2640 ggtcagtcta atgcaacgga tccagaacta gaaaaggttg catctaagct tgaaatcccg 2700 ctttcctaca taaaaatagc acaagaagat gcgactatta ttaaacagat taatgaaata 2760 gaagacttga tctatttaga tcaaatggag aaaccaaaag aattaataag taaaattaaa 2820 gatc 2824 // |
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