EBI Dbfetch

ID   Z12157; SV 1; linear; genomic DNA; STD; PRO; 1724 BP.
XX
AC   Z12157;
XX
DT   24-SEP-1992 (Rel. 33, Created)
DT   21-JUN-2006 (Rel. 88, Last updated, Version 10)
XX
DE   S.halstedii celA1 gene for endoglucanase.
XX
KW   cel1 gene; cellulase.
XX
OS   Streptomyces halstedii
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Streptomycineae; Streptomycetaceae; Streptomyces.
XX
RN   [1]
RP   1-1724
RX   PUBMED; 1400190.
RA   Fernandez-Abalos J.M., Sanchez P., Coll P.M., Villanueva J.R., Perez P.,
RA   Santamaria R.I.;
RT   "Cloning and nucleotide sequence of celA1, and
RT   endo-beta-1,4-glucanase-encoding gene from Streptomyces halstedii JM8";
RL   J. Bacteriol. 174(20):6368-6376(1992).
XX
RN   [2]
RP   1-1724
RA   Santamaria R.I.;
RT   ;
RL   Submitted (22-MAY-1992) to the EMBL/GenBank/DDBJ databases.
RL   Ramon I. Santamaria, Instituto de Microbiologia Bioquimica, C.S.I.C./
RL   Universidad, de Salamanca, Plaza de la Merced S/N, Salamanca, Salamanca,
RL   37008, Spain
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1724
FT                   /organism="Streptomyces halstedii"
FT                   /isolate="JM8(CECT3310)"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:1944"
FT   -35_signal      357..362
FT   -10_signal      382..388
FT   mRNA            392..>1411
FT   mRNA            393..>1411
FT   terminator      1415..1471
FT   protein_bind    386..414
FT                   /bound_moiety="Putative cellobiose induced regulatory
FT                   protein"
FT   CDS             446..1411
FT                   /transl_table=11
FT                   /gene="celA1"
FT                   /product="cellulase"
FT                   /EC_number="3.2.1.4"
FT                   /note="endoglucanase"
FT                   /db_xref="GOA:P33682"
FT                   /db_xref="HSSP:1TML"
FT                   /db_xref="InterPro:IPR001524"
FT                   /db_xref="UniProtKB/Swiss-Prot:P33682"
FT                   /protein_id="CAA78145.1"
FT                   /translation="MSRKLRTLMAALCALPLAFAAAPPAHAADPTTMTNGFYADPDSSA
FT                   SRWAAANPGDGRAAAINASIANTPMARWFGSWSGAIGTAAGAYAGAADGRDKLPILVAY
FT                   NIYNRDYCGGHSAGGAASPSAYADWIARFAGGIAARPAVVILEPDSLGDYGCMNPAQID
FT                   EREAMLTNALVQFNRQAPNTWVYMDAGNPRWADAATMARRLHEAGLRQAHGFSLNVSNY
FT                   ITTAENTAYGNAVNNELAARYGYTKPFVVDTSRNGNGSNGEWCNPSGRRIGTPTRTGGG
FT                   AEMLLWIKTPGESDGNCGVGSGSTAGQFLPEVAYKMIYGY"
FT   mat_peptide     526..1408
FT                   /product="cellulase"
FT   sig_peptide     446..525
XX
SQ   Sequence 1724 BP; 286 A; 633 C; 545 G; 260 T; 0 other;
     cccgggcgaa gaacggatct tcccccgaga tgaccagggc ggccgctccc gtctggcggg        60
     tggccagcgc gcgggcggtt gaattgcgtg tgtaaccgag ttgccgcacg gcccgttcga       120
     cggcctcgcg ttttgtgcgg ctcacgtgcg gtgcgttgtt gagggcacgg gaggccactg       180
     agcgggaaac accggcgtgt tcggccacct cgtcgagcgt cggatgccga cgcggcgctg       240
     cttctgccat gactcgtgag tctttcacag gctgggaggc acacattggg accgcttccg       300
     gtgcagtggc ctactgaggc ctggtcactc gggagaatcg aatgctgcgc cgcatattga       360
     cagccaaccg ccccacccgc acgattggga ccgcttccag tgggagcgct cccaaagtca       420
     cgtcctctga aatcgaggta ctgtcttgag ccgcaaactt cgtaccctga tggcagcgct       480
     ctgtgcgctg ccgctggcgt tcgccgccgc accccccgcc cacgccgccg accccaccac       540
     catgaccaac gggttctacg cggatcccga ctcgagcgcg agccggtggg cggccgccaa       600
     ccccggcgac ggccgggccg ccgcgatcaa cgcctccatc gccaacacac ccatggcccg       660
     ctggttcggc tcctggagcg gcgccatcgg caccgcggca ggcgcctacg ccggagcggc       720
     ggacggccgg gacaagctgc ccatcctcgt cgcctacaac atctacaacc gtgactactg       780
     cggcgggcac tccgccggtg gagccgcttc gccgtccgcg tacgccgact ggatcgctcg       840
     gttcgccgga ggtatcgcgg cccgcccggc cgtcgtcatc ctcgaaccgg actctctcgg       900
     agactacggc tgcatgaacc cggcccagat cgacgaacgc gaggccatgc tcaccaacgc       960
     cctcgtccag ttcaaccgcc aagctcccaa cacctgggtc tacatggatg ccggcaaccc      1020
     gcgctgggcc gacgcggcga ccatggcccg gcgtctccac gaagccggtc tgcgacaggc      1080
     ccacggcttc tcgctcaacg tctccaacta catcaccacc gccgagaaca ccgcctacgg      1140
     caacgccgtc aacaatgaac tggccgctcg ctacggctac accaagccgt tcgtcgtgga      1200
     caccagccgc aacggcaacg gctccaacgg cgagtggtgc aacccctccg gccgccgcat      1260
     cggcacgccc acccggacag gtggaggcgc cgagatgctc ctgtggatca agactccggg      1320
     cgagtccgac ggcaactgcg gtgtgggatc cggctccacg gccggccagt tcctccccga      1380
     ggtcgcctac aagatgatct acggctactg atccggggag tcccgccgtc gcccaggcga      1440
     ccgcaatccg ccggcggggc gggcactccc cccgaccatc tcaccggaga ggggccgacg      1500
     cgccccctcc ttgagagccg gccccgcgat ttcgtgaggc gggctgtccg gcagacgggc      1560
     aggagagcag aacacgcctc tgagcggcaa gaatgacgat tgtcgaggtc ttccttcctg      1620
     ccaccgtcgg aggcacctcc agggtgatta cccactcggg tgaactggcg gcctggcggc      1680
     cggtctcgtt ttcgagggga ggtccgggcc gcgatcacgg gatc                       1724
//