EBI Dbfetch

ID   Z11497; SV 1; linear; genomic DNA; STD; PRO; 1575 BP.
XX
AC   Z11497; S59339;
XX
DT   21-NOV-1991 (Rel. 30, Created)
DT   14-NOV-2006 (Rel. 89, Last updated, Version 8)
XX
DE   B.licheniformis ansA gene for asparaginase
XX
KW   asparaginase.
XX
OS   Bacillus licheniformis
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
XX
RN   [1]
RP   1-1575
RA   van Dijl J.M., de Jong A., Smith H., Bron S., Venema G.;
RT   "Lack of specific hybridization between the lep genes of salmonella
RT   typhimurium and bacillus licheniformis";
RL   FEMS Microbiol. Lett. 81:345-352(1991).
XX
RN   [2]
RP   1-1575
RA   van Dijl J.;
RT   ;
RL   Submitted (14-NOV-1991) to the EMBL/GenBank/DDBJ databases.
RL   van Dijl J., State University of Groningen, Kerklaan 30, Haren, the
RL   Netherlands.
XX
RN   [3]
RX   DOI; 10.1016/0378-1097(91)90239-7
RX   PUBMED; 1916233.
RA   van Dijl J.M., de Jong A., Smith H., Bron S., Venema G.;
RT   "Lack of specific hybridization between the lep genes of Salmonella
RT   typhimurium and Bacillus licheniformis";
RL   FEMS Microbiol. Lett. 65(3):345-351(1991).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1575
FT                   /organism="Bacillus licheniformis"
FT                   /strain="Industrial strain, Gist-brocades N.V."
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:1402"
FT   CDS             <1..371
FT                   /codon_start=3
FT                   /transl_table=11
FT                   /gene="orfA"
FT                   /function="unknown"
FT                   /db_xref="UniProtKB/Swiss-Prot:P30428"
FT                   /protein_id="CAA77573.1"
FT                   /translation="ILPEFLSLVKHGKIRMEFRAVVKEITEDELIFSVDGKETRIKNDF
FT                   VFAMTGYHPDHSFLQKMGVKIDAESGRPFFNEETMETNEEGIFIAGVIAAGNNANEIFI
FT                   ENGRFHGGLIAAEIAKRI"
FT   repeat_region   402..440
FT                   /partial
FT   RBS             465..471
FT   CDS             481..1449
FT                   /transl_table=11
FT                   /gene="ansA"
FT                   /product="Asparaginase"
FT                   /function="deamination of asparagine to  aspartate"
FT                   /note="the putative protein shows similarities  with
FT                   asparaginases of E.chrysanthemi (32.3%  identity),
FT                   A.glutaminasificans (28.1% identity),  S.cerevisiae (36%
FT                   identity) and E.coli (25%  identity)."
FT                   /db_xref="GOA:P30363"
FT                   /db_xref="HSSP:1HFW"
FT                   /db_xref="InterPro:IPR006034"
FT                   /db_xref="UniProtKB/Swiss-Prot:P30363"
FT                   /citation=[1]
FT                   /protein_id="CAA77574.1"
FT                   /translation="MNKKVALITTGGTIASRKTESGRLAAGAISGPELAEMCSLPEDVQ
FT                   IDVYPAFQLPSMHITFQHLLELKQTIERVFQDGGYDGAVVTHGTDTLEETAYFLDLTIE
FT                   DERPVVVTGSQRAPEQQGTDAYTNIRHAVYTACSPDIKGAGTVVVFNERIFNARYVKKV
FT                   HASNLQGFDVFGFGYLGIIDNDKVYVYQKLLKRDVHQLQRPLPAVDIVKCYLDGDGKFI
FT                   RAAVREGVEGIVLEGVGRGQVPPNMMADIEQALNQGVYIVITTSAEEGEVYTTYDYAGS
FT                   SYDLAKKGVILGKDYDSKKARMKLAVLLASYKEGIKDKFCY"
XX
SQ   Sequence 1575 BP; 467 A; 329 C; 405 G; 374 T; 0 other;
     ggatccttcc ggaattcctt tcgcttgtca agcacggaaa aatcagaatg gaattccggg        60
     cggtcgtcaa agaaattacg gaagacgagc tgattttttc tgtcgacgga aaagaaacca       120
     ggatcaaaaa tgattttgta tttgccatga ccggctatca tccagatcat agctttcttc       180
     aaaaaatggg agtcaaaatc gacgcagagt cgggccgtcc tttctttaat gaagaaacga       240
     tggagacgaa cgaagaaggc atctttatcg ccggcgttat tgcggccggg aacaacgcaa       300
     atgaaatctt tattgaaaac ggccgttttc acggcggctt aatcgcagct gaaattgcta       360
     aacggatata aaaaccggat gttttatgat ttgtatcagg caaaagagag catattctat       420
     atatagatgt gctctctttt ttatcggcac acagatcaac gcgaaaaggg gcatcttgac       480
     atgaataaaa aagtagctct cattacaacg ggaggaacga tcgccagccg gaaaaccgaa       540
     agcggccggc ttgcggcagg ggcgatcagc ggccctgaat tagccgaaat gtgcagcctg       600
     ccggaagatg tacagatcga tgtctacccg gcgtttcagc ttccaagtat gcatataaca       660
     tttcagcatt tattagagct caagcaaacc atcgaacgcg tgtttcagga cggcggctat       720
     gacggcgcgg ttgtaacgca tggtacggac acgcttgaag aaaccgccta ttttttggat       780
     ttaacaatag aggatgagag gccggtcgtt gtcacaggtt cacagcgagc accggaacag       840
     caaggcacag atgcatatac aaatatcagg catgccgtat atacagcttg cagtccggat       900
     ataaaaggag ccggcacggt tgttgttttt aacgaaagga tctttaacgc gcgctacgtt       960
     aaaaaagtgc acgcttcgaa tctgcagggt tttgacgtat tcggtttcgg ctacttaggg      1020
     atcattgata acgacaaagt atacgtttat caaaagctgt taaagcggga tgttcaccag      1080
     ctgcaaaggc cgcttcctgc agtggacatc gtcaaatgct atcttgatgg agacggcaag      1140
     tttatcagag ccgccgtccg cgagggtgtc gaagggatcg tccttgaggg agtcggaagg      1200
     gggcaggtgc cgccgaatat gatggctgat atcgaacaag ctttaaatca gggcgtttat      1260
     atcgtcatca cgacgagcgc agaggaaggc gaagtttata cgacctatga ctatgcaggc      1320
     agcagctatg acctcgcgaa aaaaggggtc attttaggca aggattatga cagcaaaaaa      1380
     gcgagaatga aactcgccgt ccttcttgca agctataaag aaggcataaa agacaaattc      1440
     tgctattagg cggcctgttc tttttcaatt tcaaaacaat ccgaatcatg gtaggataga      1500
     atgagatgaa aaggaaagag gtttcctcat gttcggaatt atctcagcag gcctagctcc      1560
     cggaatcgca ctgtt                                                       1575
//