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EBI DbfetchID Y15903; SV 1; linear; genomic DNA; STD; PRO; 4404 BP. XX AC Y15903; XX DT 30-JUN-1998 (Rel. 56, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 2) XX DE Streptococcus agalactiae hylB gene and partial rmlB, mutX genes XX KW 8-oxo-dGTP-nucleoside triphosphatase; dTDP-glucose-4.6-dehydratase; KW hyaluronate lyase; hylB gene; mutX gene; rmlB gene. XX OS Streptococcus agalactiae OC Bacteria; Firmicutes; Lactobacillales; Streptococcaceae; Streptococcus. XX RN [1] RX DOI; 10.1016/S0167-4781(98)00045-1 RX PUBMED; 9602074. RA Gase K., Ozegowski J., Malke H.; RT "The Streptococcus agalactiae hylB gene encoding hyaluronate lyase: RT completion of the sequence and expression analysis"; RL Biochim. Biophys. Acta 1398(1):86-98(1998). XX RN [2] RP 1-4404 RA Malke H.; RT ; RL Submitted (16-DEC-1997) to the EMBL/GenBank/DDBJ databases. RL H. Malke, Friedrich Schiller University, Institute for Molecular Biology, RL Winzerlaer Str 10, D-07745 Jena, FRG XX CC Related sequence U15050 XX FH Key Location/Qualifiers FH FT source 1..4404 FT /organism="Streptococcus agalactiae" FT /map="adjacent to locus involved in dTDP-L-rhamnose FT synthesis" FT /strain="Group B, 4755 (human strain)" FT /mol_type="genomic DNA" FT /clone_lib="E.coli plasmid library" FT /clone="TG1(pHYB1)" FT /clone="TG1(pHYB32)" FT /clone="TG1(pHYB33)" FT /clone="TG1(pHYB34)" FT /clone="TG1(pHYB35)" FT /clone="TG1(pHYB51)" FT /clone="TG1(pHYB53)" FT /clone="TG1(pHYB55)" FT /clone="TG1(pHYB100)" FT /clone="TG1(pHYB103)" FT /clone="TG1(pHYB113)" FT /clone="TG1(pHYB123)" FT /clone="TG1(pHYB124)" FT /clone="TG1(pHYB129)" FT /clone="TG1(pHYB133)" FT /db_xref="taxon:1311" FT CDS complement(<1..332) FT /transl_table=11 FT /gene="mutX" FT /product="putative 8-oxo-dGTP-nucleoside triphosphatase" FT /db_xref="GOA:O86477" FT /db_xref="HSSP:1K2E" FT /db_xref="InterPro:IPR020084" FT /db_xref="UniProtKB/TrEMBL:O86477" FT /protein_id="CAA75864.1" FT /translation="MTKLATICYIDNGKELLLLHRNKKENDVHEGKWISVGGKLEAGET FT PDECAKREILEETHLTEKKMDFKGVITFPEFTPGHDWYTYVFKVTDYEGELISDDESRE FT GTLEWVP" FT RBS complement(339..343) FT /gene="mutX" FT -10_signal complement(355..360) FT /gene="mutX" FT -35_signal complement(381..386) FT /gene="mutX" FT -35_signal 477..482 FT /gene="hylB" FT -10_signal 501..506 FT /gene="hylB" FT misc_feature 511 FT /note="major transcriptional initation site of hylB" FT RBS 539..543 FT /gene="hylB" FT sig_peptide 549..638 FT /gene="hylB" FT CDS 549..3767 FT /transl_table=11 FT /gene="hylB" FT /product="hyaluronate lyase" FT /EC_number="4.2.2.1" FT /db_xref="GOA:O86478" FT /db_xref="HSSP:1LXM" FT /db_xref="InterPro:IPR014718" FT /db_xref="UniProtKB/TrEMBL:O86478" FT /protein_id="CAA75865.1" FT /translation="MEIKKKYRIMLYSALILGTILVNNSYQAKAEELTKTTSTSQIRDT FT QTNNIEVLQTESTTVKETSTTTTQQDLSNPTASTATATATHSTMKQVVDNQTQNKELVK FT NGDFNQTNPVSGSWSHTSAREWSAWIDKENTADKSPIIQRTEQGQVSLSSDKGFRGAVT FT QKVNIDPTKKYEVKFDIETSNKAGQAFLRIMEKKDNNTRLWLSEMTSGTTNKHTLTKIY FT NPKLNVSEVTLELYYEKGTGSATFDNISMKAKGPKDSEHPQPVTTQIEESVNTALNKNY FT VFNKADYQYTLTNPSLGKIVGGILYPNATGSTTVKISDKSGKIIKEVPLSVTASTEDKF FT TKLLDKWNDVTIGNHVYDTNDSNMQKINQKLDETNAKNIKTIKLDSNHTFLWKDLDNLN FT NSAQLTATYRRLEDLAKQITNPHSTIYKNEKAIRTVKESLAWLHQNFYNVNKDIEGSAN FT WWDFEIGVPRSITATLALMNNYFTDAEIKTYTDPIEHFVPDAGYFRKTLDNPFKALGGN FT LVDMGRVKIIEGLLRKDNTIIEKTSHSLKNLFTTATKAEGFYADGSYIDHTNVAYTGAY FT GNVLIDGLTQLLPIIQETDYKISNQELDMVYKWINQSFLPLIVKGELMDMSRGRSISRE FT AASSHAAAVEVLRGFLRLANMSNEERNLDLKSTIKTIITSNKFYNVFNNLKSYSDIANM FT NKMLNDSTVATKPLKSNLSTFNSMDRLAYYNAEKDFGFALSLHSKRTLNYEGMNDENTR FT GWYTGDGMFYLYNSDQSHYSNHFWPTVNPYKMAGTTEKDAKREDTTKEFMSKHSKDAKE FT KTGQVTGTSDFVGSVKLNDHFALAAMDFTNWDRTLTAQKGWVILNDKIVFLGSNIKNTN FT GIGNVSTTIDQRKDDSKTPYTTYVNGKTIDLKQASSQQFTDTKSVFLESKEPGRNIGYI FT FFKNSTIDIERKEQTGTWNSINRTSKNTSIVSNPFITISQKHDNKGDSYGYMMVPNIDR FT TSFDKLANSKEVELLENSSKQQVIYDKNSQTWAVIKHDNQESLINNQFKMNKAGLYLVQ FT KVGNDYQNVYYQPQTMTKTDQLAI" FT terminator 3780..3811 FT /note="bidirectional transcription terminator of hylB and FT rmlB" FT CDS complement(3819..>4404) FT /codon_start=2 FT /transl_table=11 FT /gene="rmlB" FT /product="dTDP-glucose-4,6-dehydratase" FT /db_xref="GOA:O86479" FT /db_xref="HSSP:1KEP" FT /db_xref="InterPro:IPR001509" FT /db_xref="UniProtKB/TrEMBL:O86479" FT /protein_id="CAA75866.1" FT /translation="YNPSSPYSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIE FT KFIPRQITNILAGIKPKLYGEGKNVRDWIHTNDHSTGVWAILTKGRIGETYLIGADGEK FT NNKEVLELILEKMGQPKDAYDHVTDRAGHDLRYAIDSTKLREELGWEPQFTNFSEGLEE FT TIKWYTENETWWKAEKDAVEAKYAKTQEVIK" XX SQ Sequence 4404 BP; 1521 A; 859 C; 721 G; 1303 T; 0 other; ggtacccatt ctaaggttcc ttctcgtgat tcatcatctg aaattaactc tccttcataa 60 tcagttactt taaagacata ggtataccaa tcatgacctg gcgtaaattc aggaaaagtg 120 atgactcctt taaaatccat ttttttttct gttaaatggg tttcttctag aatttcacgc 180 ttagcacatt catccggagt ttcacctgcc tccagtttac cgcctaccga gatccacttt 240 ccctcatgaa catcattctc ttttttgttg cggtgcaata gaagtaattc cttgccattg 300 tcaatgtaac aaattgttgc taatttagtc atttcaaact cctctcattc tcatattata 360 acaaaaaata gcctttattt aggcaattta ctatcaaagt agcacacaaa tgattaaaca 420 aattattttg tttaccagtt atttactttt cactttgatg acattttaaa ggttctttat 480 cataaacctt taaagcatgc tataattagg aaaaaccggt ttacaatttt aaaccctaag 540 gagtaaaaat ggaaatcaaa aagaaatatc gtattatgct ttattcagcc cttattttag 600 gaacaatatt ggttaacaat agttaccaag ctaaagctga agagcttacc aaaactacct 660 caacgtccca aataagagat actcaaacta ataatattga agttctccag actgaaagta 720 ccactgtcaa agagactagc accacaacca cacaacaaga tctgtctaac cccacagctt 780 caaccgcaac tgcaacagcc actcatagca caatgaaaca agtagtagat aatcaaactc 840 aaaataagga gctggtgaaa aacggagatt ttaatcaaac taaccctgta tctggaagct 900 ggtcacatac aagcgctagg gaatggtctg cttggattga taaagaaaat actgctgata 960 aatcacctat tatccaacgt accgaacaag gccaagtaag cctatccagc gacaaaggct 1020 ttagaggtgc tgtaacacaa aaagtgaaca ttgatcccac taaaaaatat gaggtcaagt 1080 ttgatattga aacaagtaac aaggctggac aagctttcct tcgtattatg gagaaaaaag 1140 acaacaatac gcgactttgg ctttctgaga tgaccagcgg tactactaac aaacatacct 1200 taacaaagat atataaccca aagttaaatg tctccgaggt gacacttgaa ctttattatg 1260 aaaaaggaac tggttctgct acttttgata atatatcaat gaaagcaaaa ggccctaaag 1320 actcagagca tccacaaccc gtcacaacac aaattgaaga aagcgttaat acggctttaa 1380 acaaaaatta cgtttttaat aaagctgact accaatacac tctaaccaat ccgtctctcg 1440 ggaaaattgt tggtggaata ttgtatccaa acgctactgg ttcaacaact gttaaaatat 1500 ctgataaatc tggtaaaata attaaagaag taccgttatc agttacagct tcaacagaag 1560 ataagtttac aaaactcctc gacaaatgga acgacgtgac tattggtaat catgtttacg 1620 atactaatga ttcgaacatg caaaagatta atcagaaatt agatgaaact aacgccaaaa 1680 acatcaaaac tatcaaactg gattctaatc acactttcct ttggaaagat ttagataatc 1740 tcaataattc agcacagtta accgctactt atcgtcgttt ggaagattta gctaaacaaa 1800 tcaccaatcc ccactctact atttacaaaa atgaaaaagc tattcgtact gtaaaagaga 1860 gtctggcttg gcttcatcaa aacttctaca atgttaataa agatatagaa ggctctgcca 1920 attggtggga ttttgaaatc ggtgtccctc gctcaattac agctacccta gctctcatga 1980 ataactactt cactgacgct gaaataaaaa cttataccga cccaattgaa cactttgttc 2040 ctgatgcagg atatttccgt aaaacgcttg acaatccatt taaagccctt ggtggtaatc 2100 tagtcgatat ggggcgcgtt aaaatcattg aaggtttact tcgtaaagac aatactatta 2160 tcgaaaaaac ttctcattct ctaaaaaatc tttttactac tgctactaaa gctgaaggtt 2220 tctatgctga cggttcttac atcgaccata caaatgttgc ttatactggc gcctatggta 2280 atgttctgat agatggtttg acacaattgc tgcctatcat tcaagaaact gactataaaa 2340 tctctaatca agaacttgat atggtttata aatggattaa tcaatcattt ttacctttaa 2400 ttgtaaaagg tgagttaatg gatatgagtc gtggacgctc aattagtaga gaggcagctt 2460 cttcgcatgc ggctgcagtt gaagttctca gaggtttcct cagattggct aacatgtcta 2520 atgaagagcg aaacttagac ctcaaatcaa ctattaaaac gattatcact tcaaataaat 2580 tctacaatgt cttcaataac ctcaaatcgt attccgatat tgccaacatg aataagatgc 2640 ttaatgacag tacagtcgct actaaacctt taaaaagtaa tttatcaacc tttaatagca 2700 tggaccgctt agcttattat aatgccgaga aagactttgg tttcgcgctt tcattacatt 2760 ctaaacgtac cctcaactat gaaggaatga atgatgaaaa tacacgtggt tggtataccg 2820 gagatggtat gttctatctt tataatagtg atcaatctca ttatagtaat catttttggc 2880 caaccgtcaa tccttataaa atggctggaa caactgaaaa agatgctaag cgtgaagata 2940 ccactaagga attcatgagc aaacatagca aagacgctaa agaaaaaacc ggtcaagtta 3000 caggaacatc tgactttgtt ggttccgtca aacttaatga tcactttgct cttgccgcta 3060 tggattttac taactgggat cgcaccttaa cagcacaaaa aggttgggtt atcttaaatg 3120 ataagattgt ctttttaggt agcaacatca agaatactaa cggcattgga aatgtttcta 3180 caacaattga tcaacgaaaa gacgattcta aaacacctta tactacatac gtcaatggaa 3240 aaactattga tttaaaacaa gcaagttctc aacaatttac agatacaaaa agtgtctttt 3300 tagaatcaaa agaacctggt cgcaatattg gttatatctt ctttaaaaat agcactattg 3360 atattgaacg caaagagcaa acaggtactt ggaacagcat taatcgtact tctaaaaata 3420 cctcaatcgt tagcaatcct tttatcacta taagccaaaa gcatgacaac aaaggtgata 3480 gctatggtta catgatggtt ccaaacattg atcgcacaag ttttgataaa ttagccaaca 3540 gcaaagaagt agaattacta gaaaatagtt caaaacaaca agttatctat gataaaaaca 3600 gtcaaacttg ggctgttatc aaacacgata atcaagagag tctcattaac aatcaattca 3660 aaatgaataa agcgggactt tacctagtac aaaaagttgg taatgactat caaaatgtct 3720 attaccaacc tcaaaccatg acaaaaacag accaattagc tatctaacta gccgtacata 3780 aaaaacctat catcaaaatg ataggttttt tggttcaatt acttaatgac ttcttgagtt 3840 ttagcatact tggcttctac agcatctttt tctgctttcc accatgtctc attttctgta 3900 taccatttaa tagtttcttc caaaccttct gaaaagtttg taaattgtgg ttcccagccg 3960 agttcttcac gcaattttgt agaatcgata gcgtaacgaa gatcatgacc agcacggtca 4020 gttacgtgat cgtaagcgtc ttttggttga cccattttct caaggataag ttcaagaacc 4080 tccttgttgt ttttctcacc gtcggcacca ataaggtatg tttcaccaat acgaccctta 4140 gtcaaaatag cccagacacc tgttgaatgg tcatttgtgt ggatccagtc acggacgttt 4200 ttaccttcac cataaagttt tggtttgatt cctgccaaga tatttgtaat ttgacgtgga 4260 atgaattttt cgatgtgttg gtatggtcca tagttatttg aacagttcga aatggtcgct 4320 tttacgccga aggaacgtac ccatgcctta acgataaggt cagaagctgc cttagttgat 4380 gagtaaggtg atgatggatt gtat 4404 // |
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