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EBI DbfetchID Y11521; SV 1; linear; genomic DNA; STD; PRO; 3377 BP. XX AC Y11521; XX DT 21-APR-1997 (Rel. 51, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 5) XX DE Pseudomonas sp. vanA gene & vanB gene XX KW vanA gene; vanB gene; vanillate-O-demethylase. XX OS Pseudomonas sp. OC Bacteria; Proteobacteria. XX RN [1] RX PUBMED; 9098058. RA Priefert H., Rabenhorst J., Steinbuechel A.; RT "Molecular characterization of genes of Pseudomonas sp. strain HR199 RT involved in bioconversion of vanillin to protocatechuate"; RL J. Bacteriol. 179(8):2595-2607(1997). XX RN [2] RP 1-3377 RA Priefert H.; RT ; RL Submitted (25-FEB-1997) to the EMBL/GenBank/DDBJ databases. RL H. Priefert, Institut fuer Mikrobiologie, Universitaet Muenster, RL Corrensstrasse 3, D-48149, Muenster, FRG XX FH Key Location/Qualifiers FH FT source 1..3377 FT /organism="Pseudomonas sp." FT /strain="HR199 (DSM 7063)" FT /mol_type="genomic DNA" FT /db_xref="taxon:306" FT -35_signal 985..990 FT -10_signal 1009..1014 FT mRNA 1025 FT /note="transcription start site" FT RBS 1141..1144 FT /gene="vanA" FT CDS 1153..2217 FT /transl_table=11 FT /gene="vanA" FT /product="a-subunit oxygenase" FT /note="subunit of vanillate-O-demethylase" FT /db_xref="GOA:O05616" FT /db_xref="InterPro:IPR015881" FT /db_xref="InterPro:IPR017941" FT /db_xref="UniProtKB/Swiss-Prot:O05616" FT /protein_id="CAA72287.1" FT /translation="MFPKNAWYVACTPDEIADKPLGRQICNEKIVFYRGPEGRVAAVED FT FCPHRGAPLSLGFVRDGKLICGYHGLEMGCEGKTLAMPGQRVQGFPCIKSYAVEERYGF FT IWVWPGDRELADPALIHHLEWADNPEWAYGGGLYHIACDYRLMIDNLMDLTHETYVHAS FT SIGQKEIDEAPVSTRVEGDTVITSRYMDNVMAPPFWRAALRGNGLADDVPVDRWQICRF FT APPSHVLIEVGVAHAGKGGYDAPAEYKAGSIVVDFITPESDTSIWYFWGMARNFRPQGT FT ELTETIRVGQGKIFAEDLDMLEQQQRNLLAYPERQLLKLNIDAGGVQSRRVIDRILAAE FT QEAADAALIARSAS" FT CDS 2214..3167 FT /transl_table=11 FT /gene="vanB" FT /product="reductase-subunit oxygenase" FT /note="subunit of vanillate-O-demethylase" FT /db_xref="GOA:O05617" FT /db_xref="InterPro:IPR000951" FT /db_xref="InterPro:IPR001041" FT /db_xref="InterPro:IPR001433" FT /db_xref="InterPro:IPR006058" FT /db_xref="InterPro:IPR008333" FT /db_xref="InterPro:IPR012675" FT /db_xref="InterPro:IPR017927" FT /db_xref="InterPro:IPR017938" FT /db_xref="UniProtKB/Swiss-Prot:O05617" FT /protein_id="CAA72288.1" FT /translation="MIEVIISAMRLVAQDIISLEFVRADGGLLPPVEAGAHVDVHLPGG FT LIRQYSLWNQPGAQSHYCIGVLKDPASRGGSKAVHENLRVGMRVQISEPRNLFPLEEGV FT ERSLLFAGGIGITPILCMAQELAAREQDFELHYCARSTDRAAFVEWLKVCDFADHVRFH FT FDNGPDQQKLNAAALLAAEAEGTHLYVCGPGGFMGHVLDTAKEQGWADNRLHREYFAAA FT PNVSADDGSFEVRIHSTGQVLQVPADQTVSQVLDAAGIIVPVSCEQGICGTCITRVVDG FT EPDHRDFFLTDAEKAKNDQFTPCCSRAKSACLVLDL" FT repeat_region 3174..3217 FT /rpt_type=INVERTED XX SQ Sequence 3377 BP; 669 A; 905 C; 1011 G; 792 T; 0 other; agctgatcgg tcgcccaacc actccgatgt cggaagccat cgcggcagca attggccgct 60 aaaactgcat tttcgcgact tgagtgacac ctgggttaga taacccaggt gtctcgcacc 120 gctttgggtt agtggtgggc aatagcggtg tctggtcacc gcttgcccgg cggcgcgccc 180 gctattggat gattctcaac ttcctggtgc cggcgtcttg ttggggccca aacaggcggg 240 cataacgcaa tgtggcattt gcactgtcgc gcatgatggc ttctgctcga gcaccttgcc 300 cgctaatcag cgcgtctacc acagcatgat gctgcatgtt ggcaaaattg aaccggcggt 360 actcttgggg aggttgctac cgtcgacggc cagtgaactg acagaggcaa agggcaggtg 420 ttcattccga gccaatgctt cacctatggc agcgttaccg ctggcatcca cgatagcttg 480 atggaagcgc ttgttgatgt cgtggtattc ggcgaggtcg tcttcgctga cataaccttt 540 ctcaaatagg gcatcgccct gggccaagca ctgcaagagg atctcttgcg tttcactgga 600 tagccctcgc tcggcagcct gccttgcggc cagtccttca agtacccctc gaacctccac 660 cgcgcctgcc aggtcatttg gggtcatttg ccgcactgca tagccacgtg cgccttggcg 720 atcagtaacc cttcctgttc tagcgctcgg aacgcaatgc ggataggtgt gcgccgacac 780 tcccaggcgc tcggcagtgg ggatttcggc gatgcgctct cctgccggga gttcgccatc 840 cacaatcatt ttgcgcagta gattgagtac tcgctgcccg ggcccgctca tttcagcctc 900 cgattggatc cagtaatggt ttgagagaat tttactcgca agggatttct gggcaatagc 960 cccgctgatt gctggttttt gtatgtggcg tgcgactatc gcacagaatt ggatccacct 1020 tggcgcaaaa aaactggagc tacctcatcg gtcgtggtta tattggatcc cataaggtca 1080 agttcatagc tgattttggc tttagatgtc cattgtggat ccaaaaacaa gatcgccatt 1140 gaggaacgcg ccatgtttcc gaaaaacgcc tggtatgtcg cttgcactcc ggatgaaatc 1200 gcagataagc cgctaggccg tcagatctgc aacgaaaaga ttgtcttcta tcgggggccg 1260 gaaggacgtg ttgccgcggt agaggatttc tgccctcatc gcggggcacc gttgtccctg 1320 ggtttcgttc gcgacggtaa gctgatttgc ggctaccacg gtttggaaat gggctgcgag 1380 ggcaaaacgc tcgcgatgcc cgggcagcgc gttcaaggct tcccttgcat caaaagctac 1440 gcggtagaag agcgatacgg ctttatctgg gtatggcctg gtgatcgcga gctggcggat 1500 ccggcgctta ttcaccacct ggagtgggcc gataatccgg agtgggccta tggtggcggt 1560 ctctaccaca tcgcttgtga ttaccgcctg atgatcgaca acctcatgga tctcacccat 1620 gagacctatg tgcatgcctc cagcatcggt caaaaggaaa ttgacgaggc accggtcagt 1680 actcgtgtcg agggcgacac cgtgattacc agccggtaca tggataacgt catggcccct 1740 ccgttctggc gtgctgcgct tcgtggcaac ggcttggccg acgatgtacc ggttgatcgc 1800 tggcagatct gccgattcgc tcctccgagt cacgtactga tcgaagtagg tgtggctcat 1860 gcgggcaaag gcggatatga cgcgccggcg gaatacaagg ccggcagcat agtggtcgac 1920 ttcatcacgc cggagagtga tacctcgatt tggtacttct ggggcatggc tcgcaacttc 1980 cgtccgcagg gcacggagct gactgaaacc attcgtgttg gtcagggcaa gatttttgcc 2040 gaggacctgg acatgctgga gcagcagcag cgcaatctgc tggcctaccc ggagcgccag 2100 ttgctcaagc tgaatatcga tgccggcggg gttcagtcac ggcgcgtcat tgatcggatt 2160 ctcgcagctg aacaagaggc cgcagacgca gcgctgatcg cgagaagtgc atcatgattg 2220 aggtaatcat ttcggcgatg cgcttggttg ctcaggacat cattagcctt gagtttgtcc 2280 gggctgacgg tggcttgctt ccgcctgtcg aggccggcgc ccacgtcgat gtgcatcttc 2340 ctggcggcct gattcggcag tactcgctct ggaatcaacc aggggcgcag agccattact 2400 gcatcggtgt tctgaaggac ccggcgtctc gtggtggttc gaaggcggtg cacgagaatc 2460 ttcgcgtcgg gatgcgcgtg caaattagcg agccgaggaa cctattccca ttggaagagg 2520 gggtggagcg gagtctgctg ttcgcgggcg ggattggcat tacgccgatt ctgtgtatgg 2580 ctcaagaatt agcagcacgc gagcaagatt tcgagttgca ttattgcgcg cgttcgaccg 2640 accgagcggc gttcgttgaa tggcttaagg tttgcgactt tgctgatcac gtacgtttcc 2700 actttgacaa tggcccggat cagcaaaaac tgaatgccgc agcgctgcta gcggccgagg 2760 ccgaaggtac ccacctttat gtctgtgggc ccggcgggtt catggggcat gtgcttgata 2820 ccgcgaagga gcagggctgg gctgacaatc gactgcatcg agagtatttc gccgcggcgc 2880 cgaatgtgag tgctgacgat ggcagtttcg aggtgcggat tcacagcacc ggacaagtgc 2940 ttcaggtccc cgcggatcaa acggtctccc aggtgctcga tgcggccgga attatcgttc 3000 ccgtttcttg tgagcagggc atctgcggta cttgcatcac tcgggtggta gacggagagc 3060 ctgatcatcg tgacttcttc ctcacggatg cggagaaggc aaagaacgac cagttcaccc 3120 cctgttgctc gcgagccaag agcgcctgtt tggtcttgga tctctaactc atccccgtgt 3180 ccggtcccct gctttggtgc ggcggactgt gcgcgggtaa gtaaacaggc tcaaccgttt 3240 ttagcgggat aaccattctt gaggatgaag gagggttatc ccgctctttt catgcaccaa 3300 gccattcata gtcaccagct gcttctacgt gctgctgcgt tacaagttta ttcagaagga 3360 aatcggaatg atcaaat 3377 // |
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