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EBI DbfetchID Y08695; SV 1; linear; genomic DNA; STD; PRO; 3159 BP. XX AC Y08695; XX DT 10-OCT-1996 (Rel. 49, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 16) XX DE Clostridium tertium nanH gene XX KW acylneuraminate lyase; nanH gene; neuraminidase; siaH gene; sialidase. XX OS Clostridium tertium OC Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; OC Clostridium. XX RN [1] RX PUBMED; 9832614. RA Grobe K., Sartori B., Traving C., Schauer R., Roggentin P.; RT "Enzymatic and molecular properties of the Clostridium tertium sialidase"; RL J. Biochem. 124(6):1101-1110(1998). XX RN [2] RC Revised by [3] RA Grobe K.; RT ; RL Submitted (24-SEP-1996) to the EMBL/GenBank/DDBJ databases. RL K. Grobe, Forschungszentrum Borstel, Parkallee 22, 23845 Borstel, FRG XX RN [3] RP 1-3159 RA Grobe K.; RT ; RL Submitted (08-SEP-1997) to the EMBL/GenBank/DDBJ databases. RL K. Grobe, Forschungszentrum Borstel, Parkallee 22, 23845 Borstel, FRG XX DR CABRI; DSM 2485. XX FH Key Location/Qualifiers FH FT source 1..3159 FT /organism="Clostridium tertium" FT /strain="DSM 2485" FT /mol_type="genomic DNA" FT /cell_line="E.coli K12 Jm101" FT /db_xref="taxon:1559" FT -10_signal 109..114 FT RBS 116..125 FT CDS 129..2450 FT /transl_table=11 FT /gene="nanH (siaH)" FT /product="sialidase (neuraminidase)" FT /function="cleaves terminally bound sialic acid from FT various substrates" FT /EC_number="3.2.1.18" FT /db_xref="GOA:P77848" FT /db_xref="HSSP:2SLI" FT /db_xref="InterPro:IPR002860" FT /db_xref="InterPro:IPR004124" FT /db_xref="InterPro:IPR008985" FT /db_xref="InterPro:IPR011040" FT /db_xref="InterPro:IPR013320" FT /db_xref="UniProtKB/TrEMBL:P77848" FT /protein_id="CAA69951.1" FT /translation="MYSLIKKSISTIALSTLAITSLPTYSVSSQTTEEYGARKYFINNN FT IENIKNIENKSFDLIQNLNTKILEKENIETLSGTVVDFTKEATSNSTIPNGLIIEKSNI FT NITAGKGYDLSSEMGSEYVKALEKGTIIVSYKSTSNNSIQSLVSIGNNTSGNRDRHFHI FT YITNTGEVGMELRNTDSVLKYTLGRPAAVRSIYKNNLVFNTIAFKADPSNKQYKLFANG FT ELLATLNTDVYKFINDITGVNNVMLGGTVRDGVIAYPFGGTIGNVKIYNEILTDEALKA FT ETGATTYGKNIFYAGDSTKSNYFRIPSLLSLRSGTVVSAADARYGGTHDSKSNIDIAFS FT KSLDGGIIWKNPTIPLQFNDYVARNIDWPRDSIGKNVQIQGSASFIDPVLLEDKETKRL FT FIFADAMPAGIGSSNASTGSGYKDIAGKKYMKLRWHQDGSSTYNYSIRENGVIYNDVTN FT LPTEYKIDGDYNLYKNGIALLYKQYDYNFSGTTLLETATNIDVNMNVFYKDSLFKVFPT FT TYLDMKYSDDEGETWSNLNIVSSFKPENSKFLVLGPGVGKQISKGQYKGRLIVPLYSSS FT YAELGFMYSDDHGQTWNYVAADNRNTGTTAEAQIVEMPDGSLKSYLRTGSGVIAEVTSI FT NGGETWSDRVTVPNMHTTSYGTQLSVINYAGLIDGKEAIILSAPDSSSARRNGKIWIGL FT ISDTGASGINKYSIEWKYCYSVDSSNMGYSYSCLTELPNGDIGLLYEKYDSWSRNELHL FT KNILKYETFSINELKQPISN" FT RBS 2468..2474 FT CDS 2481..>3159 FT /transl_table=11 FT /product="putative acylneuraminate lyase" FT /EC_number="4.1.3.3" FT /note="monomer" FT /db_xref="GOA:O32375" FT /db_xref="HSSP:1NAL" FT /db_xref="InterPro:IPR002220" FT /db_xref="InterPro:IPR013785" FT /db_xref="UniProtKB/TrEMBL:O32375" FT /protein_id="CAA69950.1" FT /translation="MRNLEKYKGIIPAFYACYDDEGKISPERTQMFTQYLIDKGVKGLY FT VCGSSGECIYQSKEERKITLENVMKVAKGKITIIAHVGCNNTRDSEELAEHAESIGVDA FT IASIPPIYFHLPDYSIAEYWNDISNAAPNTDFIIYNIPQLAGVGLGINLYKQMLKNPRV FT IGVKNSSMPVQDIQMFKDISGDESVVFNGPDEQFVAGRIMGADGGIGGTYAVMPELFLA FT ADKA" XX SQ Sequence 3159 BP; 1220 A; 368 C; 595 G; 976 T; 0 other; ctgcagatgc attaactaaa tctcgttatg aaaatagaaa agtaaaatta aatgagattg 60 tttctaaata aatagatatt acagtgtagt tgtagactga gaatataaat aaaattgagg 120 agaagattat gtatagttta ataaaaaaat caatatctac tattgcttta tcaacattag 180 cgattacttc tttacctact tatagtgtat caagtcaaac tacagaagag tatggagcaa 240 gaaaatattt tataaataac aatatagaaa atataaaaaa tatagaaaat aaatcttttg 300 atttgataca aaatttaaat actaaaatat tagaaaaaga aaatatagag actctaagtg 360 gaacggttgt agattttact aaagaggcta catcaaattc aactattcct aatggattaa 420 ttattgaaaa aagtaatatt aatattacag caggaaaagg ttatgacttg agttctgaaa 480 tgggctcgga atatgtaaaa gctttagaaa aaggaacaat aattgtttct tataaatcaa 540 caagtaataa tagtatacag tcattagtaa gcattggaaa taatactagc gggaataggg 600 atagacattt ccatatttat attactaata caggggaagt gggaatggaa cttagaaata 660 ctgatagtgt attaaaatat acattaggta ggccagctgc tgtaagatca atatacaaaa 720 ataatcttgt atttaacaca attgcattta aagcagatcc aagcaataag caatataaat 780 tatttgccaa tggtgaatta cttgcaactt taaataccga tgtatataag tttataaatg 840 atattacagg tgtaaataat gttatgctag gtgggactgt tagagatgga gttatagcat 900 atccatttgg aggaacaatt ggaaatgtga aaatttataa tgagatttta actgatgagg 960 cccttaaggc agaaactgga gcaacaacat atgggaaaaa cattttttat gctggtgata 1020 gtactaaatc taattatttt agaataccat cattattatc tttaagatca ggaactgttg 1080 tatcagctgc tgatgcaaga tatggtggaa cacatgattc taaaagtaat atagatattg 1140 cattttcaaa gagtctagat ggaggtataa tatggaaaaa tccaacaata ccattacaat 1200 ttaatgatta tgttgcaaga aatatagatt ggccacgtga ttctatagga aaaaatgttc 1260 aaattcaagg aagtgcatct tttattgacc cagtattact agaagataag gaaacaaaga 1320 gattgtttat ttttgcagat gctatgcctg ctggaatagg aagttctaat gcatcaacgg 1380 gatcaggata taaggatatt gctggtaaga aatatatgaa gttaagatgg caccaagatg 1440 gaagttcaac ttataattat tccatccgtg aaaatggggt aatatataat gatgtaacta 1500 acttacctac agagtataaa attgatggag attataattt atataaaaat ggaatcgcat 1560 tattatataa acaatatgac tataatttta gtggtacaac tctattggag acagccacaa 1620 atattgatgt taacatgaac gtattttata aagattctct atttaaagta tttccaacaa 1680 catatttaga catgaagtat agcgatgatg aaggagaaac ttggtctaat ttgaacattg 1740 taagtagctt taaaccggaa aattctaaat tcttagtttt aggaccaggt gttggaaaac 1800 aaatttcaaa gggacagtat aaaggaagat taattgtacc actatattca agttcatatg 1860 ctgagcttgg ctttatgtat agtgatgatc atggacaaac ttggaattat gtagcagcag 1920 acaataggaa caccggtact acagcagaag cacaaattgt tgaaatgcct gatggatctc 1980 ttaaatcata tttgcgtact gggtctggag ttatagcaga agtcacaagt atcaatggag 2040 gagaaacttg gagcgataga gtaactgtac ctaatatgca tactacttca tatggaactc 2100 aactatcagt tattaattat gcagggctga ttgatggaaa agaagctatt attctatcag 2160 caccagattc aagtagtgca cgtagaaatg gaaagatatg gataggttta attagtgata 2220 caggtgcttc aggaataaat aaatattcta ttgaatggaa atattgttat tcagtagata 2280 gttcaaatat gggatattca tattcttgtc ttacagaatt acctaatgga gatattggat 2340 tactatatga gaagtatgac tcttggtctc gtaatgaatt gcatcttaaa aatatattaa 2400 aatatgaaac attttcaatt aatgagttaa agcaacctat atctaactaa gattagtaga 2460 aaatattaag gagaagattt atgagaaatt tagaaaagta taaaggtata ataccagctt 2520 tttatgcttg ttatgatgat gaggggaaaa taagtccaga acgtacacag atgtttactc 2580 aatacttaat agataaggga gtaaaaggcc tttacgtttg tggatcttca ggggaatgta 2640 tatatcaaag taaagaagaa agaaaaataa cattagaaaa tgtaatgaaa gttgcaaaag 2700 gaaaaattac tataatagct catgtaggat gtaacaatac tagagatagt gaagaacttg 2760 cagaacatgc agaaagtata ggggtagatg ctatagcatc tattccccca atttacttcc 2820 atttacctga ttattcaatt gctgaatatt ggaatgatat aagcaatgct gcaccaaata 2880 ctgattttat tatatataat attccacaat tagcgggagt tggtttagga attaatttat 2940 ataagcaaat gcttaaaaat ccaagagtaa ttggtgtaaa gaattcttca atgccagtac 3000 aagatattca aatgtttaag gatatatctg gtgatgaaag cgttgtattt aatggaccag 3060 atgagcaatt tgttgctgga cgtataatgg gagcagatgg aggtattggc ggaacttatg 3120 cagttatgcc agaattgttt cttgcagctg acaaagctt 3159 // |
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