 |
|
EBI DbfetchID X99622; SV 1; linear; genomic DNA; STD; PRO; 7224 BP. XX AC X99622; XX DT 08-JAN-1997 (Rel. 50, Created) DT 04-MAY-2005 (Rel. 83, Last updated, Version 15) XX DE Rhodococcus opacus catR, catA, catB, catC genes and five ORFs XX KW catA gene; catB gene; catC gene; catechol 1,2-dioxygenase; KW glucose-6-phosphate dehydrogenase; muconate cycloisomerase; KW muconolactone isomerase. XX OS Rhodococcus opacus OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; OC Corynebacterineae; Nocardiaceae; Rhodococcus. XX RN [1] RX PUBMED; 8990288. RA Eulberg D., Golovleva L.A., Schloemann M.; RT "Characterization of catechol catabolic genes from Rhodococcus erythropolis RT 1CP"; RL J. Bacteriol. 179(2):370-381(1997). XX RN [2] RC revised by [3] RA Schloemann M.; RT ; RL Submitted (29-JUL-1996) to the EMBL/GenBank/DDBJ databases. RL M. Schloemann, University Of Stuttgart, Institute For Microbiology, RL Allmandring 31, Stuttgart, 70569, FRG XX RN [3] RP 1-7224 RA Eulberg D.; RT ; RL Submitted (12-SEP-1997) to the EMBL/GenBank/DDBJ databases. RL D. Eulberg, University Of Stuttgart, Institute For Microbiology, RL Allmandring 31, Stuttgart, 70569, FRG XX FH Key Location/Qualifiers FH FT source 1..7224 FT /organism="Rhodococcus opacus" FT /strain="1CP" FT /mol_type="genomic DNA" FT /db_xref="taxon:37919" FT CDS complement(<1..487) FT /transl_table=11 FT /gene="orf1" FT /product="hypothetical protein" FT /note="homologous to bacterial proteins involved in copper FT resistance" FT /db_xref="GOA:O33536" FT /db_xref="HSSP:1GYC" FT /db_xref="InterPro:IPR008972" FT /db_xref="InterPro:IPR011707" FT /db_xref="InterPro:IPR017909" FT /db_xref="UniProtKB/TrEMBL:O33536" FT /protein_id="CAA67937.1" FT /translation="MPGNRPLDVQLSRRRVLQWGAGAAFLGLTGACARTDDTEAPIGPD FT SPEVRAFAEAESRRFPGGRPVAYELSATQSEVDIAGKRTAAWLYNTQLPGPILRANVGD FT RVQVRFRNQLPDSTTVHWHGLAIRNDMDGVPDVTQAPIPADTEFVYDFIPPDPGTYWY" FT CDS complement(540..1487) FT /transl_table=11 FT /gene="orf2" FT /product="hypothetical protein" FT /db_xref="InterPro:IPR006683" FT /db_xref="UniProtKB/TrEMBL:O33537" FT /protein_id="CAA67938.1" FT /translation="MTTPPRSITLRFLAAPTEVATLGGAVQGGRILEWIDKAAYACAVG FT WSGSYCVTAYVGNIKFTRSIESGHLVEVEARLVHTGRSSMHIQCTVSSADPRTGQFTEA FT SNCLIIFVAVDDSGKPTSVPPWQPVTATDRAESEEAVMRIGLRADIEDAMSKQNYTDAG FT TAPESLTRFLAAPTDVNWGGKTHGGTVMRWIDEAAYLCGTSWNGTPCVSVYAGGVRFYR FT PIQIGHVVEVDARLLHTGAQTMHISVHVRSGDPRTREMNLTTHCLTVVAAVDEDGAATA FT VQQWVPQSDEDRKLEAHARELIALRDRARIGALA" FT CDS 1721..2257 FT /transl_table=11 FT /gene="dle" FT /product="dioxygenase-like enzyme" FT /db_xref="GOA:O33538" FT /db_xref="InterPro:IPR000627" FT /db_xref="InterPro:IPR015889" FT /db_xref="UniProtKB/TrEMBL:O33538" FT /protein_id="CAA67939.1" FT /translation="MTSTSTTTAPHVDQACLDHVDREFYLPAQRVLPWRANLAALPGEP FT GPPLRIAGVVRSRDGAPLSGAELEIWHADADGCYSGYSVEILRNNLRGIVVTCAGGRFD FT ISTVQPAPCEFSSARGEADVGHVNVVVHARGFRPLETRLVPTPGASREVDASAVESLVG FT LVGGEVSCDFVLDPI" FT CDS complement(2271..3041) FT /transl_table=11 FT /gene="catR" FT /product="putative regulator of catechol degradative FT operon" FT /db_xref="GOA:O33539" FT /db_xref="InterPro:IPR005471" FT /db_xref="InterPro:IPR012794" FT /db_xref="InterPro:IPR014757" FT /db_xref="PDB:2G7U" FT /db_xref="UniProtKB/TrEMBL:O33539" FT /protein_id="CAA67940.1" FT /translation="MTESDRDYIQSIERGFAVLLAFDAQRPNPTLAELATEAGLSRPAV FT RRILLTLQKLGYVAGSGGRWSLTPRVLSIGQHYSESHALIEAAMPRLLEVAEKTQESAS FT LGVLDGADVVYAARVPVRRIMSINVSVGTRVPAYATSMGRALLAWAPADVVEQVVAEST FT FEKLGPETISTAAELDKELAKVREQGFALTSEELEKGLISLPAPVRDAGGTVVGVVACS FT TSSARNTPAQFREQAVPCVLAAAAALSTDMGFAG" FT CDS 3250..4092 FT /codon_start=1 FT /transl_table=11 FT /gene="catA" FT /product="catechol 1,2-dioxygenase" FT /EC_number="1.13.11.1" FT /db_xref="GOA:P95607" FT /db_xref="InterPro:IPR000627" FT /db_xref="InterPro:IPR007535" FT /db_xref="InterPro:IPR012800" FT /db_xref="InterPro:IPR015889" FT /db_xref="UniProtKB/Swiss-Prot:P95607" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA67941.1" FT /translation="MTTTESPTAAGSGSAATDKFKAERATADTSPERLAAIAKDALGAL FT NDVILKHGVTYPEYRVFKQWLIDVGEGGEWPLFLDVFIEHSVEEVLARSRKGTMGSIEG FT PYYIENSPELPSKCTLPMREEDEKITPLVFSGQVTDLDGNGLAGAKVELWHADNDGYYS FT QFAPHLPEWNLRGTIIADEEGRYEITTIQPAPYQIPTDGPTGQFIEAQNGHPWRPAHLH FT LIVSAPGKESVTTQLYFKGGEWIDSDVASATKPELILDPKTGDDGKNYVTYNFVLDPA" FT CDS 4122..5243 FT /transl_table=11 FT /gene="catB" FT /product="muconate cycloisomerase" FT /EC_number="5.5.1.1" FT /db_xref="GOA:P95608" FT /db_xref="InterPro:IPR001354" FT /db_xref="InterPro:IPR013341" FT /db_xref="InterPro:IPR013342" FT /db_xref="InterPro:IPR013370" FT /db_xref="InterPro:IPR018110" FT /db_xref="UniProtKB/Swiss-Prot:P95608" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA67934.1" FT /translation="MTDLSIVSVETTILDVPLVRPHKFATTSMTAQPLLLVAVTTAGGV FT TGYGEGVVPGGPWWGGESVETMQAIVERYIVPVLLGRGVDEITGIMPDIERVVANARFA FT KAAVDVALHDAWARSLGVPVHTLLGGAFRKSVDVTWALGAAPAEEIIEEALDLVESKRH FT FSFKLKMGALDPAVDTARVVQIAQALQGKAGVRIDVNARWDRLTALKYVPRLVEGGVEL FT IEQPTPGEQLEVLAELNRLVPVPVMADESVQTPHDALEVARRGAADVIALKTTKCGGLQ FT KSREVVAIAKAAGIACHGATSIEGPIGTAASIHFACAEPGIDFGTELFGPLLFSEELLQ FT EPIRYADGQVFLPEGPGLGVELNMDAVKTWTRN" FT CDS 5259..5540 FT /transl_table=11 FT /gene="catC" FT /product="muconolactone isomerase" FT /EC_number="5.3.3.4" FT /db_xref="GOA:P95609" FT /db_xref="InterPro:IPR003464" FT /db_xref="InterPro:IPR011008" FT /db_xref="UniProtKB/Swiss-Prot:P95609" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA67935.1" FT /translation="MALFHVRMDVAIPRDLDPKVRDETIAKEKAYSQELQRSGKWPEIW FT RIVGQYSNISIFDVESADELHEILWNLPLFPYMNIEIMPLTKHGSDVK" FT CDS complement(5590..6783) FT /transl_table=11 FT /gene="orf8" FT /note="homologous to regulators of NagC /XylR family" FT /db_xref="GOA:P95610" FT /db_xref="InterPro:IPR000600" FT /db_xref="InterPro:IPR019885" FT /db_xref="UniProtKB/TrEMBL:P95610" FT /protein_id="CAA67936.1" FT /translation="MRTPRSTTSPATGGDVFALIRAGQATTRTEIGTLTGLSRTAVAAR FT VTSLQASGLVVEREEGVSTGGRPPVKLSFHVQAGVVLSAAIGRSRTQLAICDLAGDVIV FT AEDVEQEIGTSPAELMPVIAQRLIALRESLGETAGRTVGIGISLPGTVDTERGCSLNSP FT MMSGWDGVPLQPFLADVTDAPVFVDNDANVMVLAERRGERRDFADMLLIKASTGLGAGL FT VSGGVLQRGALGAAGEIGHTKTAAAQGVVCRCGDVGCIEAVAGGWALVRNLQEQGREVT FT HIRDVITLALAGDAEARRMIRESGRQIGEVLSGAINLLNPEVVIVGGDMAQAYDTFVAG FT LRETLYGNAIAVATQQLQILPWTHGELSGVVGSATMALDHVLSVPAVDQLLAGAPRP" FT CDS 6935..>7224 FT /transl_table=11 FT /gene="orf9" FT /note="homologous to N-termini of glucose-6-phosphate FT dehydrogenases" FT /db_xref="GOA:P95611" FT /db_xref="HSSP:1DPG" FT /db_xref="InterPro:IPR001282" FT /db_xref="InterPro:IPR016040" FT /db_xref="UniProtKB/TrEMBL:P95611" FT /protein_id="CAA67933.1" FT /translation="MSPATTVEPCDFVVFGGTGDLAVRKLLPALYLRDRDGQLPAETRV FT IGASRAGLDTDGYRDKVRGELARFVSSDQLDSDTCERFLNRLEYVSVDAADP" XX SQ Sequence 7224 BP; 1202 A; 2443 C; 2432 G; 1147 T; 0 other; ggtaccagta cgtgcccggg tcgggcggga tgaagtcgta gacgaactct gtgtccgccg 60 gtatcggagc ctgcgtgaca tccgggaccc cgtccatgtc gttgcggatg gccagcccgt 120 gccagtgcac ggtggtggaa tccggcagct ggttgcgaaa ccgcacctgg accctgtccc 180 cgacgttcgc ccgcaggatc gggcccggca gctgcgtgtt gtacaaccac gcagcagtgc 240 gtttacctgc gatgtcgacc tcggactgtg tcgcggagag ctcgtaggcg accggcctgc 300 cgccgggaaa tcgccgggac tcggcctcgg cgaacgcgcg cacctccggg gagtcgggtc 360 cgatcggggc ctcggtgtcg tcggttcggg cgcaggcccc ggtgagtccg aggaatgcag 420 caccggctcc ccactgcagc acccgccgcc gggacaactg cacgtcgagt ggacggttcc 480 caggcatccc cccgctcctt ccgcgggtag accgtttccc gtgacgctac tcgggcggat 540 tacgccaggg cgccgattcg cgcacggtcg cggagggcga tgagttcgcg ggcgtgcgcc 600 tccagcttgc ggtcctcgtc ggactgcggc acccattgct gcactgcggt tgcggcgccg 660 tcctcgtcga cggcggcgac gacggtgagg cagtgggtgg tgaggttcat ttcccgggtg 720 cgcgggtcgc cggatcgcac gtgcaccgag atgtgcatgg tctgcgcgcc ggtgtgcagc 780 aggcgggcgt cgacctcgac gacgtgaccg atctggatcg ggcggtagaa gcgcaccccg 840 ccggcgtaca cggacacgca cggcgtgccg ttccacgacg tgccgcacag gtaggcggcc 900 tcgtcgatcc agcgcattac ggttccaccg tgcgtcttgc cgccccagtt gacgtcggtg 960 ggcgcggcga ggaagcgggt gagcgattcc ggtgcggtgc ccgcgtcggt gtagttctgc 1020 ttgctcatcg cgtcttcgat gtcggcccgc aggccgatgc gcatgaccgc ctcctcggac 1080 tcggcgcggt cggtggcggt gacgggctgc cacggcggca cgctcgtcgg cttaccggag 1140 tcgtcgacgg cgacgaagat gatcaggcag ttgctggcct cggtgaactg tccggtgcgc 1200 gggtcggccg acgacacggt gcactggatg tgcatgctcg agcgcccggt gtggacgagc 1260 cgggcttcga cctcgacgag gtgacccgac tcgatggacc gggtgaactt gatgtttccc 1320 acgtaggcgg tgacgcaata gctcccgctc cagccgaccg cgcaggcgta ggcggccttg 1380 tcgatccatt cgaggatccg gccgccctgg acggctcctc cgagcgtcgc cacttcggtg 1440 ggtgcggcga ggaagcggag ggtgatgctg cggggaggcg tggtcatcga tgtgatcctc 1500 ggggattcga cgtggccggc acgagggcgc gaaccgggga cggcgaaacg tcgttgtccg 1560 ccggtggcgg ggaaattatc ccacagatgc accggaccga cccgtccgcg atgtgaacgg 1620 accggaggtt gacgaacgcg tgtgaccacg cgcacactca acaacgaccg cacagcgttc 1680 tcaacgtacg cacagcgtac gagagtgatg ggatcaggtg atgacctcga cgtcgacgac 1740 gaccgcacct cacgtggacc aggcttgcct cgaccacgtc gaccgcgagt tctacctccc 1800 cgcgcagcga gtgctgccgt ggagggcgaa ccttgcggca ctccccggcg aaccggggcc 1860 tccgctccgc atcgcggggg tcgtgcgctc acgcgacggc gcccctttgt cgggggcgga 1920 actcgagatc tggcacgcgg acgccgacgg ctgctactcc gggtactccg tcgagattct 1980 gcgcaacaac ctgcgcggca tcgtcgtcac atgcgccggt ggccgcttcg acatctcgac 2040 cgtgcagccg gcgccgtgcg aattctcgag cgcccgaggc gaagccgatg tcgggcacgt 2100 caacgtcgtc gtgcatgcgc gtggcttccg accgctcgag acgcgcctgg tgccgacacc 2160 gggcgcatcg cgagaagtgg acgcgtccgc cgtcgagtcg ctcgtcggcc tggtcggcgg 2220 agaggtctcc tgcgacttcg tgctcgaccc gatctgaccg agtgatgccg tcagccggcg 2280 aagcccatgt ccgtgctcag ggcggccgcg gcggcgagga cgcacggaac ggcctgttcc 2340 cggaactgtg ccggagtatt ccgcgcggac gacgtggagc aggcgaccac gccgaccact 2400 gtgccgccgg cgtcgcggac gggggcgggc agcgagatca gccccttctc cagctcctcg 2460 gacgtcaggg cgaacccctg ctcgcggacc ttggcgagtt ccttgtcgag ttcggctgca 2520 gtgctgatgg tttcgggccc gagtttctcg aacgtggact cggcgaccac ctgctcgacc 2580 acgtcggcgg gggcccaggc caggagcgcg cgacccatgg acgtggcgta cgcggggacg 2640 cgggtgccga cagagacgtt gatgctcatg atccgccgga cgggaacgcg cgccgcgtag 2700 accacgtcgg cgccgtcgag cacgccgagc gacgcggact cctgggtctt ctcggccacc 2760 tccagcagtc ggggcatcgc ggcctcgatc agtgcgtgcg actccgagta gtgctgtccg 2820 atgctcagca cgcggggggt gagcgaccat cggccacccg agccggcgac gtaccccagc 2880 ttctgcaggg tgagcaggat ccggcggact gcggggcgcg acagtcccgc ttctgtggcg 2940 agttcggcga gcgtcgggtt cggacgctgc gcgtcgaacg cgaggagcac tgcgaacccg 3000 cgctcgatgc tctggatgta atcgcgatcg ctctcggtca ttgcggcctc caccagcgat 3060 tcgggtgcgt attcggactt ggggttgaca tttcaatgtg actgcgcgca cagtagtggt 3120 gtacgcacag cgtgatgcta gtacgcagag cgtacagaag gcaagaccct cggcgcgtga 3180 acacccaccg cgtcgcccaa aactcgatat cagcccaatc cgctcccctc ccagcccaag 3240 gagaaacaga tgaccaccac cgagagcccc accgccgccg gatccggctc cgccgccacc 3300 gacaagttca aggccgagcg cgccacagcc gacacctcgc cggagcggct cgcggccatc 3360 gccaaggacg cactcggcgc actcaacgac gtgatcctga agcacggcgt cacctacccc 3420 gagtaccgcg tgttcaagca gtggctcatc gacgtgggcg agggcggcga atggccgctg 3480 ttcctcgacg tgttcatcga gcactcggtc gaagaagtcc tcgctcgcag ccgcaagggc 3540 accatgggca gcatcgaggg cccgtactac atcgagaact cgcccgagct gccgtcgaag 3600 tgcaccctgc cgatgcggga agaggacgag aagatcaccc cgctcgtctt ctccggccag 3660 gtcaccgatc tcgacggcaa cggcctcgcc ggcgcgaagg tcgaactgtg gcacgccgac 3720 aacgacggct actactccca gttcgccccg cacctgcccg agtggaacct gcgcggcacc 3780 atcatcgccg acgaggaggg ccgctacgag atcaccacga tccagccggc gccctaccag 3840 atcccgaccg acggcccgac cggtcagttc atcgaggcgc agaacggtca cccgtggcgt 3900 cccgcgcacc tgcacctcat cgtgtcggcg ccgggcaagg agtcggtgac cacgcagctg 3960 tacttcaagg gtggcgagtg gatcgacagc gacgtcgcgt cggctaccaa gccggaactc 4020 atcctcgatc cgaagaccgg tgacgacggc aagaactacg tcacctacaa cttcgtgctc 4080 gaccccgcgt gacccgttcc gcccgtagag aaagtgatcc gatgaccgac ctgtcgatcg 4140 tctccgtcga aacgacgatc ctcgatgtgc cgttggtgcg tccccacaag ttcgccacca 4200 cgtcgatgac ggcgcagccg ctgctgctcg tcgccgtcac gaccgcgggc ggcgtgaccg 4260 gttacggcga gggcgtcgtc cccggcggcc cgtggtgggg cggcgaatcg gtggagacga 4320 tgcaggcgat cgtcgagcgg tacatagtcc cggtcctgct ggggcgtggt gtcgacgaga 4380 tcaccggcat catgccggac atcgaacgag tcgtcgccaa tgcacgtttc gcgaaagccg 4440 ccgtcgacgt cgcgttgcac gacgcctggg cccgaagcct gggtgtgccc gtgcacacgc 4500 tgctcggcgg cgccttccga aagagtgtcg acgtcacgtg ggccctcggc gccgcacccg 4560 ccgaggagat catcgaggag gccctcgatc tcgtcgagtc gaagcggcac ttcagtttca 4620 aactgaagat gggtgcgctc gatcccgccg tcgacactgc ccgggtggtc cagatcgcgc 4680 aggccctgca gggcaaggcc ggcgtccgca tcgacgtcaa cgcccgctgg gacaggctca 4740 ccgcgctgaa gtacgtgccc cgcctcgtcg aaggcggcgt cgaactgatc gagcagccca 4800 cacccggtga gcaactcgag gtcctcgccg agctcaaccg actcgtcccc gtcccggtga 4860 tggcggacga gagcgtgcag actccgcacg acgccctcga ggtcgcacgc cgcggcgccg 4920 cggacgtcat cgccctcaag accaccaaat gtggcggcct gcagaagagc cgcgaggtgg 4980 tcgcaattgc caaggcagcc ggaatcgcct gccacggagc cacgtccatc gaaggcccga 5040 tcgggacggc ggcgtccatc cacttcgcgt gcgcggaacc cggcatcgac ttcggtaccg 5100 aactgttcgg accgctgctg ttcagcgagg agttgctgca ggaaccgatc cgctacgccg 5160 acggccaggt gttcctgccc gaggggcccg gactcggagt cgagctcaac atggacgcag 5220 tcaagacctg gaccaggaac taaggagcac accacccgat ggcactcttt cacgtccgca 5280 tggacgtcgc cattccccgt gacctcgacc cgaaggtgcg ggacgagacc atcgccaagg 5340 agaaggcgta ctcgcaggag ctccaacgct ccggcaagtg gcccgagatc tggcgcatcg 5400 tggggcagta cagcaacatc agcatcttcg acgtcgagtc ggccgacgaa ctgcacgaga 5460 tcctgtggaa ccttccactg ttcccgtaca tgaacatcga gatcatgccg ctcaccaagc 5520 acggttccga cgtcaaatag gccgtccggg cccgtgcgtg gtcagcgagt ctctggactc 5580 gtcaaccgct cacgggcgcg gagcacctgc gagcagctgg tccaccgcgg gcacgctgag 5640 gacgtggtcg agtgccatgg tggcgctacc gacgacgccc gacagttccc cgtgcgtcca 5700 ggggaggatc tgaagctgtt gggtggcaac ggcgatggcg ttgccgtaga gggtttcgcg 5760 tagccccgcg acgaacgtgt cgtaggcctg cgccatgtcg ccgccgacga tcacgacctc 5820 cgggttgagg aggttgatcg cgcccgacag gacctcgccg atctgccgcc cgctctcacg 5880 gatcatccgc cgcgcctcgg cgtccccggc gagggcgagg gtgatcacgt cgcggatgtg 5940 ggtgacttcg cgtccctgct cctgcagatt gcggaccagt gcccacccgc cggccacggc 6000 ctcgatacaa cccacatcac cgcagcggca caccacacct tgtgccgctg cggttttcgt 6060 gtgcccgatt tcgcccgccg cgccgagggc gccccgctgg agcacgccac cggacacgag 6120 tccggctccc agtccggtcg acgccttgat gagcagcatg tcggcgaagt cgcgacgttc 6180 tccgcgccgc tccgccagca ccatgacgtt ggcgtcgttg tcgacgaaga cgggcgcgtc 6240 ggtgacgtcg gcgaggaacg gctggagtgg gacgccgtcc cagcccgaca tcatggggga 6300 gttcaggctg cacccgcgtt cggtgtcgac ggtcccgggg aggctgatcc cgatcccgac 6360 cgtccgtccc gcggtctcgc ccagactctc gcgcagcgcg atgaggcgtt gcgcgatgac 6420 gggcatcagt tcggccgggc tggtgccgat ctcctgctcc acgtcctcgg cgacgatgac 6480 gtcgcctgcg aggtcgcaga tggccagctg ggtgcggctc cgaccgatgg ccgccgacag 6540 cacgacaccg gcctgaacgt gaaacgagag cttgaccggc ggccgaccgc cggtggagac 6600 gccctcctcg cgttcgacga cgagccccga ggcctgcagc gaagtcaccc gggccgcgac 6660 agcggtgcgg gagaggccgg tgagggttcc gatctcggtg cgcgtcgttg cttgccctgc 6720 gcgaatgagg gcgaagacgt cgccacccgt cgccggggag gtcgtcgagc gaggcgtccg 6780 catgcaggcc attcaaccaa ccggacacgc gtctgacaac gtaaggccgt gacctttgtt 6840 cgcagaaata cttaagtctg tattgatcaa aagccaaagg tcgcctactg tcgtctgtga 6900 cggcccacac atccggcttt cagagggaga tgacatgtca ccagcgacca ccgtggaacc 6960 gtgcgatttc gtcgtgttcg gcggaaccgg cgacctggcg gttcgtaagc tgcttcccgc 7020 gctctacctg cgcgatcggg acggccagct gcccgccgaa acccgcgtga tcggtgcctc 7080 gcgagccggt ctcgacaccg acggctaccg cgacaaggtg cggggagaac tcgcgcggtt 7140 cgtctccagc gatcagctgg attcggacac ctgtgagcga tttctcaatc gcctcgaata 7200 cgtctccgtc gacgccgcgg atcc 7224 // |
|