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EBI DbfetchID X98339; SV 1; linear; genomic DNA; STD; PRO; 4366 BP. XX AC X98339; XX DT 04-SEP-1996 (Rel. 49, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Anabaena variabilis avaIM gene, avaIR gene and gene encoding glucanase XX KW avaIM gene; avaIR gene; DNA methyltransferase; glucanase; KW restriction endonuclease. XX OS Anabaena variabilis OC Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Anabaena. XX RN [1] RA Lunnen K.D., Moran L.S., Slatko B.E., Wilson G.G.; RT "Cloning and sequence comparison of Ava1 and BsoB1 restriction-modification RT system"; RL Unpublished. XX RN [2] RP 1-4366 RA Wilson G.G.; RT ; RL Submitted (05-JUN-1996) to the EMBL/GenBank/DDBJ databases. RL G.G. Wilson, New England Biolabs, Inc., Research, 32 Tozer Road, Beverly, RL MA 01915, USA XX FH Key Location/Qualifiers FH FT source 1..4366 FT /organism="Anabaena variabilis" FT /mol_type="genomic DNA" FT /db_xref="taxon:1172" FT CDS 325..1365 FT /transl_table=11 FT /product="glucanase" FT /db_xref="InterPro:IPR008007" FT /db_xref="UniProtKB/TrEMBL:Q79A01" FT /protein_id="CAA66983.1" FT /translation="MWNYNELFENIEELVMHHSPSGAETEINQLLLQKFAALGVEVWSD FT RADNIIAKIPGKNSDIAVAITAHKDEIGAIVKNIGDAGKVEVRKLGGAYPWVYGEGVVD FT LLGDNETISGILSFGSRHVSHESPQKVQQEDTAVKWENAWIETKRTTEELEAAGIRPGT FT RMVIGKHRKRPVRLQDHIASYTLDNKASVAILLALVQQLKQPAIDVYLVASAKEEVGAI FT GALFFTQNQQLDALIALEICPLSEEYPVKDSENPVLLVQDAYGIYDEGLNGELRQCAKQ FT LNMSVQLTTLSGFGSDASIAMKFGHVGRAACLAFPTQNTHGYEIAHLGAISNCINLLKA FT FCETEF" FT CDS 1575..3023 FT /transl_table=11 FT /gene="avaIM" FT /product="methylase" FT /function="cytosine modification an 5'CYCGRG3'" FT /db_xref="GOA:P0A462" FT /db_xref="InterPro:IPR017985" FT /db_xref="UniProtKB/Swiss-Prot:P0A462" FT /protein_id="CAA66984.1" FT /translation="MTSFELESPIEIKTDPTDLDQESDSFVQEISRFNKALEQRFRDKM FT RLHESLSRKIVSFQANKSKPQYRWFKYKEAFSVDLVNQLIFEYEKKSFERILDPFAGAG FT TMLFACSDAGIQADGIEVLPIGQEIIEVRKIIQRQFRREDFLRLIEWYKQKPWNQHNNR FT KYLNRLRITDGAYPPETEASIERFLFSIEKENILVKQVLRFALLCILESISYTRKDGQY FT LRWDKRAFRKSGSDKFDKGKILDFDEAITEQIKLILNDSFDLISNTLFCYGTQRSGINL FT FNASCLKILPEFEQDFYDCIITSPPYCNRYDYTRTYALELALLGVGERDIVQLRQDMLS FT CTVENKEKSLIHNWQEALRILDKQELLQSILRFLERELERKKLNNNGIPRMIKGYFYEM FT ACVIIECFRVLKNGSPLFMVNDNVRYAGIDISVDLILSNIAEEIGFNVEKILVLPTGKG FT NSSQQMGTHGRKTLRKCVYVWRKP" FT CDS 3023..3970 FT /transl_table=11 FT /gene="avaIR" FT /product="restriction endonuclease" FT /function="cleaves 5'CYCGRG3' between first and second FT base" FT /db_xref="GOA:P0A458" FT /db_xref="HSSP:1DC1" FT /db_xref="InterPro:IPR015277" FT /db_xref="UniProtKB/Swiss-Prot:P0A458" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA66985.1" FT /translation="MPYQYHIQSNDDLVTPYQEVRAGFVALALERNRKATPFVEQARAL FT KIRVSQIERPQDLLQMRDIRPTLLAASGVSDKAAGHLQEQDKVDAIEGLIQNFLEPAGE FT NFVEELVYRFLLTRGDTLGGSMRNVGGILAERKFARYIISALTLSNTSYKWLDKNSKTW FT LNQPDDDTDIELRLRGLSWNLEGRNRTFIYNVNVPIVRKNIDICLFDCRQNEIEKNIIS FT NPNIYIALGELKGGIDPAGADEHWKTANSALARIRTAFDRHSLKPYTFFVGSAIEKSMA FT EEIWHQLNSGILTNAANLTQPDQVASLCAWFIQL" XX SQ Sequence 4366 BP; 1463 A; 703 C; 871 G; 1329 T; 0 other; gtcataaatt agtagcagaa tcagtcttag cggcaacaac tcctgagtct tcacccacaa 60 ttggtgtact atttctaggt gtattgggtg caatgagcaa caccaaacgc ttacagagaa 120 aatcaattaa attttctgtt cagaaaaaag gttaatacta gataattagc tgtctcttgc 180 tcattcattt atctgaatga gcgctatttt ttaagtatat aaacaccata aaatacccat 240 gcagttctag ttgaaatagc ttgtccagac aatatattga atataatggg atatagctga 300 tatagagatt aaataaaagc attgatgtgg aattacaacg aattatttga aaatatcgaa 360 gaattagtga tgcaccattc ccctagtggt gcagaaaccg aaattaatca acttttgtta 420 caaaaatttg cagcgctggg tgtggaagtt tggagcgatc gcgccgataa tatcatagcc 480 aagattccgg ggaagaactc tgatatagca gtagcgatta cagcccacaa agacgaaata 540 ggggcgatcg tcaaaaatat cggtgatgca ggaaaagtgg aagtccgcaa gcttggcggt 600 gcttaccctt gggtttatgg tgagggtgtc gttgatttat taggagacaa cgaaaccatt 660 agcggtattc tcagttttgg ttcccgccac gtttcccacg aatcaccgca gaaagtccaa 720 caagaagaca cagccgttaa atgggaaaac gcctggattg aaaccaaacg cactaccgag 780 gaattagagg ctgctgggat tcgtccaggg acaagaatgg taattggcaa acatcgcaaa 840 cgtcccgtga gactacagga tcacattgct agttatacat tagataacaa agcatcagta 900 gcgattttgt tggctttagt tcaacaatta aaacagccag caattgatgt ttatttagta 960 gcatcggcta aagaagaagt aggcgcaatt ggggcattat ttttcaccca aaatcagcaa 1020 ttagatgcct taatcgcttt agaaatttgt cccctatctg aagaatatcc tgttaaagat 1080 agtgaaaatc ccgtactttt agttcaggat gcctatggga tatatgatga gggattaaat 1140 ggagaactgc ggcaatgtgc gaagcaattg aatatgtctg tgcagctaac taccttaagt 1200 ggctttggta gtgatgcttc aattgcgatg aaatttggtc atgtgggacg tgctgcgtgt 1260 ttggcatttc ctacccaaaa cacccacggt tatgaaattg ctcatttggg tgcaatctcc 1320 aactgtatca atttattgaa agctttttgc gaaactgagt tttagggaaa ataatattat 1380 tatttacaat aaatcataga aagaatgtat tcaatattta acttgattac tgtagaagta 1440 taacaaagta taatcaggtt ctaactgttg tcaattagtc tataaaaaat agggttcaaa 1500 tcttaagtga tagacgatag tgctttgtcc tgatagaatc ttaagttacc tctttgttac 1560 aagaaaaata taaaatgact tcatttgagc ttgagagtcc aatagaaata aagactgacc 1620 cgactgatct tgatcaagag agtgattcct ttgtacaaga aatttctcga ttcaataaag 1680 cacttgagca acgttttaga gataagatgc gattgcatga aagtttaagt cgaaaaatag 1740 ttagttttca agctaataag tcaaaacctc agtatcgctg gtttaaatat aaagaagctt 1800 tttcagttga tttggtaaat cagttaatat tcgagtacga gaaaaaatca tttgagagga 1860 ttcttgaccc cttcgcagga gcaggaacaa tgctatttgc ctgtagtgat gccggtattc 1920 aagcagatgg tatagaagtg ttacctattg gtcaagagat tattgaagta aggaaaataa 1980 tccagcgaca attccgtcga gaagattttt tgagattgat tgaatggtac aaacaaaaac 2040 cttggaatca gcataataat agaaaatatc ttaatcgttt aagaattact gacggagctt 2100 atcctcctga aacagaagca tcaatagaga gatttttatt ttctatagaa aaagagaata 2160 ttcttgtgaa acaagttctc cgttttgctc tattgtgtat tcttgaatct atcagctata 2220 cccgtaaaga tggacagtat ctacgttggg ataaaagagc atttaggaaa agtggatcag 2280 ataaatttga taaaggtaaa attctggatt tcgatgaagc aattactgag caaataaaat 2340 taattttgaa tgattccttt gacttaataa gtaatacatt attttgttat gggactcaaa 2400 gaagtggaat taatttattt aatgcttcat gtcttaaaat tctgcctgaa tttgagcaag 2460 atttttacga ctgtatcatt acctctccac cctattgtaa tcgttatgac tatacacgta 2520 catacgctct agaattagct ctattaggtg tgggagaaag agatatagta caacttaggc 2580 aagatatgct gagttgtact gttgaaaaca aagaaaagtc tcttattcac aattggcagg 2640 aagcattacg catacttgat aaacaagaat tgttacaaag tatcttgcgc tttcttgagc 2700 gagagcttga aagaaaaaaa cttaataata acggtattcc tcgtatgata aaaggatatt 2760 tctatgaaat ggcttgcgtt attatagaat gctttagagt tttaaaaaat ggctcacctt 2820 tatttatggt aaatgataat gttcgctatg caggtattga tatttcggtt gatttaattc 2880 tttctaatat tgcagaagaa attggtttta atgtggagaa aattcttgtc ttacctactg 2940 gcaaaggtaa cagtagccaa caaatgggga cacatggaag aaagacactt cgcaaatgtg 3000 tgtatgtttg gagaaaaccc tagtgccata tcaatatcat attcaaagca atgatgatct 3060 tgtgactcca tatcaagaag tccgagcagg atttgttgct ttagctttag aaagaaatcg 3120 aaaagcaaca ccatttgttg agcaggcaag agcattaaag atccgagtaa gccaaattga 3180 aaggcctcaa gaccttttac aaatgaggga tattagacct actctactag ctgcctctgg 3240 ggtatcagat aaagctgctg gacaccttca agaacaagat aaagtcgatg caatagaagg 3300 tttaattcaa aacttcctag agcctgctgg agaaaacttt gtagaagaac tagtttaccg 3360 tttcctgctt acgcgtggag atacacttgg tggatcaatg cgaaatgttg gtggtatcct 3420 tgctgaacgt aaatttgcaa gatatattat ttctgcacta actttatcga atacctctta 3480 taaatggcta gataaaaaca gcaaaacttg gcttaatcaa cctgatgatg atacagacat 3540 tgaattacgt cttaggggat taagttggaa cttggagggt aggaatagga ctttcattta 3600 taatgtaaat gttccgattg tgcgaaaaaa cattgatatt tgtttgtttg attgcagaca 3660 aaatgaaatt gaaaagaata taatttcaaa cccaaatatc tacatagctc taggtgaatt 3720 aaagggcggt attgacccag caggagcaga cgaacattgg aaaacagcga acagtgcttt 3780 agccagaatc agaacagcat ttgatcggca cagtcttaag ccatatactt tttttgtggg 3840 atctgctata gaaaaaagca tggcagaaga aatttggcat caactaaatt ctggcattct 3900 tactaatgca gcaaacctca ctcagcctga ccaagttgcc tctttgtgcg cttggtttat 3960 tcaactttga tgtacagtga ctaattattt gtcactgtac acaagatgta cagtgactaa 4020 ttatttgtca ctgtacacaa gatgtacagt gactaattgt ttgtcgtcgt gacaaattaa 4080 tgtcgtcgaa tgaatccttg caatacaaag gttttagcta tttaagagtt attacattat 4140 ttctcatacg tgactaaata aatgtcgttt tccgcaaaaa tgacaaatta actgtcgctt 4200 cagtaattac aaaaaaggtt ttgtatattt tcataatgac aaattgactg tcgttttctc 4260 cacgtttaga ataacattat gtatttataa attacctttg tttcatgaac aaaaaatctt 4320 atgtctgatt ttactgttca taccgcagtg gatactgcgg aagctt 4366 // |
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