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EBI DbfetchID X98287; SV 1; linear; genomic DNA; STD; PRO; 3688 BP. XX AC X98287; XX DT 03-SEP-1996 (Rel. 49, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 16) XX DE B.stearothermophilus BsoBI restriction-modification genes XX KW bsoBIM gene; bsoBIR gene; endonuclease; methylase; KW restriction-modification enzyme. XX OS Geobacillus stearothermophilus OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Geobacillus. XX RN [1] RX DOI; 10.1007/s004380050278 RX PUBMED; 8917312. RA Ruan H., Lunnen K.D., Scott M.E., Moran L.S., Slatko B.E., Pelletier J.J., RA Hess E.J., Benner J., Wilson G.G., Xu S.Y.; RT "Cloning and sequence comparison of AvaI and BsoBI restriction-modification RT systems"; RL Mol. Gen. Genet. 252(6):695-699(1996). XX RN [2] RC revised by [3] RA Xu S.; RT ; RL Submitted (04-JUN-1996) to the EMBL/GenBank/DDBJ databases. RL S. Xu, New England Biolabs, Inc., Research, 32 Tozer Road, Beverly, MA RL 01915, USA XX RN [3] RP 1-3688 RA Xu S.; RT ; RL Submitted (20-MAY-1997) to the EMBL/GenBank/DDBJ databases. RL S. Xu, New England Biolabs, Inc., Research, 32 Tozer Road, Beverly, MA RL 01915, USA XX RN [4] RX DOI; 10.1016/S0378-1119(96)00773-1 RX PUBMED; 9099856. RA Ruan H., Lunnen K.D., Pelletier J.J., Xu S.Y.; RT "Overexpression of BsoBI restriction endonuclease in E. coli, purification RT of the recombinant BsoBI, and identification of catalytic residues of BsoBI RT by random mutagenesis"; RL Gene 188(1):35-39(1997). XX FH Key Location/Qualifiers FH FT source 1..3688 FT /organism="Geobacillus stearothermophilus" FT /lab_host="E.coli" FT /strain="JN209" FT /mol_type="genomic DNA" FT /db_xref="taxon:1422" FT CDS 1035..2006 FT /transl_table=11 FT /gene="bsoBIR" FT /product="BsoBI restriction endonuclease" FT /function="cleave 5'CYCGRG3' between first and second base" FT /db_xref="GOA:P70985" FT /db_xref="InterPro:IPR011335" FT /db_xref="InterPro:IPR011544" FT /db_xref="InterPro:IPR015277" FT /db_xref="PDB:1DC1" FT /db_xref="UniProtKB/Swiss-Prot:P70985" FT /protein_id="CAA66932.1" FT /translation="MNTQKPFENHLKSVDDLKTTYEEYRAGFIAFALEKNKRSTPYIER FT ARALKVAASVAKTPKDLLYLEDIQDALLYASGISDKAKKFLTEDDKKESINNLIENFLE FT PAGEEFIDELIFRYLLFQGDSLGGTMRNIAGALAQQKLTRAIISALDIANIPYKWLDSR FT DKKYTNWMDKPEDDYELETFAKGISWTINGKHRTLMYNITVPLVKKNVDICLFNCEPEI FT YTPQKVHQQPEKYLLLGELKGGIDPAGADEHWKTANTALTRIRNKFSEKGLSPKTIFIG FT AAIEHSMAEEIWDQLQSGSLTNSANLTKTEQVGSLCRWIINI" FT CDS 2030..3559 FT /transl_table=11 FT /gene="bsoBIM" FT /product="BsoBI methylase" FT /function="N4 cytosine modification of 5'CYCGRG3'" FT /db_xref="GOA:P70986" FT /db_xref="InterPro:IPR002941" FT /db_xref="InterPro:IPR017985" FT /db_xref="UniProtKB/Swiss-Prot:P70986" FT /protein_id="CAA66933.1" FT /translation="METVQMSLDLQPEDTKANIECSSPDYEIKCFSHNQFAPQIERLKI FT EKKYIHIVEETETFNRKLVSYQANKNQSIHNWIRYKEGFSSELVQNLIEEFGLSKGDTI FT LDPFLGSGTTSLTAKMLGINSIGIDILPISHIAFEPKSFIFEYNLEELDRAYKEIYEIS FT PTKIEQKFNHLSITEGAFPEETENDLLFFTHWDNNSQYSYQTKTLIKLILVSILEEISY FT TRKDGQYLRWDYRSQKVIETNKKRLEQGKEPIKTILDKGELPTVKESLLNTLLTIKEDI FT KEIQQKCLPNESVHELIKDSALNALPKINDNTFDAVITSPPYCNRYDYTRTYALELAYL FT GVDEKKIRELRQAQLSCTVENKSKLKQLKDYYHSLFLESRYAEIERLVTGNEVLNEINY FT ALRKRWENGEVNNKGILSMVDGYFTELTFIFYELFRTCKPGAKVAFVNDNVRYAGEIIP FT VDFLSTKIAEDIGFKPIKIYTLKQRKGNSSQQMGKYGRVALRKSITIWEKP" XX SQ Sequence 3688 BP; 1348 A; 611 C; 688 G; 1031 T; 10 other; tcttcaagcg ttggctgttt gacaatgtta tgtctttcaa gcgcccgttc gataaaatct 60 tcaatcgcta aaaaggcaat gcgaccgttt aaaaatgctg cgaccgcttc ttcattggca 120 gggttcaata ccgtcggcaa agaaccgcct gtttnggccg cttcatatgc aaattttaaa 180 caacgataac ggtcaaaatc aattgattgg aaatgaagcg ttccgatttc tgccaaattt 240 agcttttttg nttgtgaaag ctctaatcga tccggttacc ttcatttcca ccttgaaaaa 300 tgttcctttg tcataaccct actgaatggg atgttagcat gattaggaaa atgaacaggg 360 aagagttagc acaagctatt aactatgacc ctgatgaact tactaagatt gtaaaaaacc 420 tgagaaagaa gggattaatc atgacaactg aaagttgtgg gaatttaatc tattatgtgc 480 acccagattt aatgtacggg cgagatgatg atagagaaaa cgagtatata rrcggtttac 540 gagctatgtt tgaagaacat atgannnnag tatatttgtt atatgttaca gacatcattt 600 gtacgtgcgg ggaaaaccga ccgttttagg cattttatac ggggaaaacc gactcttaca 660 aaattcttct aaaccttaga gcaacaaggg ctttgccatt ttctcgttgg taaagactct 720 aataagtctt gtcgttgata taacaaaaat tttaaaatag gctagtatat gtgtgtagcc 780 cctccgctat gctccgtggc tacacacaaa cattagcggg gatagctgac gctatccccc 840 tttctcttct ttacattatg aattaaaaca taaagtctag agagatttta tataggggca 900 cgagcggagc gagtcgnccc gngcatcttg accgagcgaa gcgagggcaa gtgcaccaat 960 aagctaataa ttcattttgt gattttatga tagaataaat taataaatat ttacgtttta 1020 taggaggaag tactttgaac actcaaaaac cttttgaaaa tcatctaaaa agtgtagacg 1080 atttaaaaac gacatatgaa gaatacagag caggatttat tgcttttgct ttagagaaaa 1140 ataaacgctc taccccatat attgagagag cacgagcatt aaaagttgct gcatctgttg 1200 caaaaacacc aaaagattta ctttacttgg aagatattca agacgctctc ctttatgcgt 1260 cgggtatttc tgataaagca aagaaattct taacggaaga tgataaaaag gagtctataa 1320 ataatttaat tgagaacttt ttggaacctg ccggagagga gtttatagac gagttaatat 1380 tccgctattt attattccaa ggagactccc taggaggaac aatgagaaac atagcaggtg 1440 cactcgctca acaaaaatta acaagagcaa taatctctgc cttggatatt gctaatatcc 1500 cttataaatg gttagactca cgagataaaa agtacactaa ttggatggac aagcctgagg 1560 atgattacga attagaaaca tttgctaaag gtattagctg gactattaac ggaaaacaca 1620 gaactttaat gtataatatt actgttccac ttgtaaaaaa gaatgttgat atatgtttat 1680 ttaactgtga acctgaaata tatacgccac aaaaagtaca ccaacagcct gaaaagtatc 1740 tactgcttgg agagttaaag ggcggaatag accctgctgg agcggatgaa cattggaaaa 1800 cagcaaatac tgctcttact aggattagaa ataaattctc cgaaaaggga ttatcaccta 1860 aaacaatttt tataggcgct gctattgagc atagtatggc agaagaaata tgggaccaac 1920 ttcaatccgg ttcattaaca aactcagcaa atcttactaa aactgaacaa gtaggctctc 1980 tttgtagatg gataattaat atataatcat atggataggg aggagtatcg tggaaaccgt 2040 acaaatgagc ttggatttac aaccagaaga cacaaaagct aatattgagt gttcttctcc 2100 tgattacgaa ataaagtgtt tctcccataa tcaatttgct cctcaaattg agcgtttaaa 2160 aattgaaaaa aaatacattc atattgtaga agagacagaa acatttaaca gaaaattagt 2220 gagttaccaa gccaacaaga accaatcaat ccacaattgg attagatata aagaggggtt 2280 ctcctccgag ttggttcaaa atctaattga agagtttgga ttatcaaaag gtgacactat 2340 attagaccct tttctaggct caggaactac aagtctaact gcaaaaatgc tcggaataaa 2400 tagtattgga atagacattt taccaatttc acacatagca tttgaaccca aatcctttat 2460 ctttgaatat aatctcgaag aactagatag ggcctataaa gaaatatacg aaatatcacc 2520 aacgaagata gaacaaaagt ttaatcattt aagtataact gagggcgctt ttccagaaga 2580 aacagaaaat gaccttttat tttttaccca ttgggataat aactcacagt atagttatca 2640 aaccaaaacg ttaataaaat taatcttggt aagcatacta gaagagatta gctatactag 2700 aaaagatggg caatatttaa gatgggatta tagatcacag aaggttatcg aaactaataa 2760 aaaaagactt gaacaaggaa aagagccaat taaaaccata ttagataagg gcgaacttcc 2820 tactgtgaaa gagtcccttt taaacactct tttaacaata aaagaagata taaaagaaat 2880 acaacaaaaa tgtttaccta atgagtccgt tcatgaactt ataaaagaca gtgcacttaa 2940 tgcactacct aaaattaatg ataatacttt tgacgctgtt attacttccc ccccatattg 3000 caatagatac gactacacta gaacatatgc acttgaactt gcttacttag gggtagatga 3060 aaagaagata agagaattaa gacaagcaca actttcgtgt acagtagaaa ataaatctaa 3120 acttaaacaa ctaaaggact actaccattc acttttccta gaaagtagat acgcagaaat 3180 tgaacgactt gtaacaggaa atgaggtatt aaatgaaatt aattacgctt taaggaagcg 3240 ctgggaaaac ggtgaggtta ataacaaggg tatactttca atggttgacg gatattttac 3300 agagttaacc tttatatttt atgaactatt tagaacgtgt aagccaggag ctaaagttgc 3360 ctttgttaat gataatgttc ggtatgcagg tgaaattatc cctgttgatt tcttatcaac 3420 gaaaattgca gaagatatcg gttttaaacc aattaaaatc tatacactaa aacaaagaaa 3480 aggcaatagc agtcaacaaa tgggtaagta tggcagagta gcgttaagaa aaagcatcac 3540 aatatgggaa aaaccataag agtgagcaca gtctcactct ctttttattt ttatcgcaac 3600 ttatccccca catataacga ttactatggc ggaaggggga agaaagatga taattgaaag 3660 aatatttgca ggaacagagg atttcatg 3688 // |
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