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EBI DbfetchID X95584; SV 1; linear; genomic DNA; STD; FUN; 2968 BP. XX AC X95584; XX DT 28-JAN-1997 (Rel. 50, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 10) XX DE A.bisporus pruA gene XX KW 1-pyrroline-5-carboxylate dehydrogenase; pruA gene. XX OS Agaricus bisporus OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Agaricomycetidae; Agaricales; Agaricaceae; Agaricus. XX RN [1] RX PUBMED; 8979339. RA Schaap P.J., Muller Y., Sonnenberg A.S.M., van Griensven L.J.L.D., RA Visser J.; RT "The Agaricus bisporus pruA gene encodes a cytosolic delta RT 1-pyrroline-5-carboxylate dehydrogenase which is expressed in fruit bodies RT but not in gill tissue"; RL Appl. Environ. Microbiol. 63(1):57-62(1997). XX RN [2] RP 1-2968 RA Schaap P.J.; RT ; RL Submitted (07-FEB-1996) to the EMBL/GenBank/DDBJ databases. RL P.J. Schaap, Wageningen Agricultural University, Section Molecular Genetics RL of Industrial Microorganisms, Dreijenlaan 2, 6703 HA, Wageningen, THE RL NETHERLANDS. XX FH Key Location/Qualifiers FH FT source 1..2968 FT /organism="Agaricus bisporus" FT /chromosome="4" FT /strain="Horst 39" FT /mol_type="genomic DNA" FT /db_xref="taxon:5341" FT CDS join(556..612,666..773,833..1111,1167..1252,1307..1758, FT 1800..1941,1994..2177,2227..2310,2365..2613) FT /gene="pruA" FT /product="1-pyrroline-5-carboxylate dehydrogenase" FT /function="proline degradation" FT /EC_number="1.5.1.12" FT /db_xref="GOA:P78568" FT /db_xref="InterPro:IPR016162" FT /db_xref="UniProtKB/Swiss-Prot:P78568" FT /protein_id="CAA64836.1" FT /translation="MATAQLATFKIPPVSNEPMLSYAPGSPERAGLQAALAEMQSQLPF FT EVPCIINGQEVRTNNIQKQPMPHDHARHLCTFHEGSPELVEKATCGALQAKDGWETMPW FT NDRAAIFLKAADLASGKYRYKLMAATMLGQGKNTWQAEIDAAAELADFFRFGVSYVEEL FT YAQQPPKNAPGCWNRTEYRPLEGFVLAVSPFNFTAIGGNLPGSPALVGNVVVWKPAPAA FT TYSNYLVFKILSEAGVPPGVIQFIPGGAEIVQAAIQSPNFRSLHFTGSTNVFKSLWKDI FT SSNLDKYKVYPRIVGETGGKNWHVIHKSAEVRNAVLQSVRGAFEYQGQKCSALSRLYVS FT RSVWENGFKTQYLEEIAKIKVGPCLDWNNYMGPVIGRRAYDNITGFIKKAKEEGGEVLI FT GGSGDDSKGFFIQPTVILTKVPRSTTMVGEIFGPVVTAYVFEDSDYEKTLELIDTTSIY FT GLTGAIFASERQALLTATNRSRNAAGNIYYNEKCTGAVVGQQPFGGARGSGTNDKAGSI FT SIFYRFVSARSIKENFVGLEDFHYPSNLV" FT mRNA join(<556..612,666..773,833..1111,1167..1252,1307..1758, FT 1800..1941,1994..2177,2227..2310,2365..>2613) FT exon 666..773 FT /number=2 FT exon 833..1111 FT /number=3 FT exon 1167..1252 FT /number=4 FT exon 1307..1758 FT /number=5 FT exon 1800..1941 FT /number=6 FT exon 1994..2177 FT /number=7 FT exon 2227..2310 FT /number=8 FT intron 613..665 FT /number=1 FT intron 774..832 FT /number=2 FT intron 1112..1166 FT /number=3 FT intron 1253..1306 FT /number=4 FT intron 1759..1799 FT /number=5 FT intron 1942..1993 FT /number=6 FT intron 2178..2226 FT /number=7 FT intron 2311..2364 FT /number=8 FT exon <556..612 FT /number=1 FT exon 2365..>2613 FT /number=9 FT polyA_site 2678 XX SQ Sequence 2968 BP; 709 A; 735 C; 761 G; 763 T; 0 other; gtcgactgcc gcgattgtac tagtttacga gacattccgc atccgagaag attatcgcgt 60 catccccggg aggtaaaggg tcttgtgaga gtgacgggtg ggtgttatcg atggatagaa 120 atcgatttct tcgggccgca gagttgtatg aggcgtggaa gaccaggagg gtgaaaaagc 180 ggcgtctgtg cgcagcagtg aggctcatag tttgaccgaa gaagcgtccc ttcaaggggc 240 agatttgaac caagggagtg tcttcttgct agggttttca tggcagtaaa cacgcgctcc 300 atgccggagg tggttgaacg gaataatgga gcgtgaatgg agctcgagat atggaatcca 360 agtccgtgag gcttccatca atgaactaga atcgtcgccg agttccttaa aacatgtctg 420 gtccgaagtt ttttggggat atgatgatcc ttccggatta taatttatct accccggaag 480 catgcgagcc atgaggagct aggtagatta taaaactaga cattccacca tatccgatct 540 ttccttatct ccatcatggc cactgctcaa ctcgcgactt tcaagatccc ccctgtgtcc 600 aatgaaccca tggtgcgcga cgcgttcatg tcttgttcct tgctatgctc atcgctccga 660 tatagctatc ctatgccccc ggctcgccgg agagagcggg tctgcaagca gcattggcag 720 aaatgcaaag ccagctaccc tttgaggttc cttgtatcat caatggccaa gaggtctgcg 780 attgcacaga ctgctttcac tcgcgtcgtt gacctttatc gtcgtgaaac aggtgagaac 840 gaacaacatc cagaaacagc ctatgccaca cgaccatgct cgccacctgt gtactttcca 900 cgaaggatca ccagaactgg tcgaaaaggc tacctgtggc gctcttcaag ccaaagacgg 960 ttgggaaacc atgccatgga acgatcgtgc tgcgattttc ctcaaggccg ccgatctcgc 1020 aagtggaaag tatcgttaca agctcatggc ggcgactatg ctcggccaag gcaaaaatac 1080 atggcaggcg gagatagacg ctgcggcgga ggttagcccc tatctttcca atttaagctc 1140 gaatcatttg gtgatgtttg cttcagttgg cagacttctt ccgcttcgga gtctcttatg 1200 tagaggaact atacgcccaa cagccgccga agaatgctcc cgggtgttgg aagtaagtct 1260 taccccacta ccatccacga ctataagcac tgagctctcc ttttagccgt accgagtacc 1320 gcccgcttga agggttcgtc cttgctgttt cgcccttcaa ctttaccgct attggtggga 1380 atctacccgg atctcccgca cttgttggaa atgtcgtcgt atggaagcct gctccagccg 1440 cgacttattc caactacctc gtcttcaaga ttctttcgga ggccggcgtt ccccccggtg 1500 tcattcaatt tattcccgga ggagctgaaa tagtacaagc tgccatccag agccctaact 1560 ttcgttcgct tcacttcact ggcagtacta atgtcttcaa atcgctctgg aaggatatct 1620 cctcgaatct agacaagtac aaggtctacc ccagaatcgt cggagagacc ggtgggaaga 1680 actggcacgt cattcacaag tctgcggaag tgagaaacgc tgtgctgcag agtgttcgag 1740 gagcattcga atatcaaggt cgggtattgt gttcattcat gttgacgttg tgctgacagg 1800 tcaaaagtgc tccgccctct cgcgtctcta cgtgtccaga tcagtctggg aaaacggttt 1860 caagacacag tacctcgagg aaattgccaa gatcaaggtt ggcccttgtc tcgactggaa 1920 caactacatg ggacctgtta tgtacgtgcg atacttattc gttataactc ttggctgacg 1980 ttgggggttg cagtggccgt cgggcatatg ataatatcac cggttttatc aagaaggcga 2040 aggaagaggg aggagaggtg ttgattggtg gatcaggtga tgactccaag ggattcttca 2100 ttcaaccaac tgtcattctt accaaggttc ctcgatctac taccatggtt ggggagatct 2160 ttgggcccgt cgtgactgta tgtttcgctc ctttgtttat atggagttct tactaaggat 2220 cgtcaggcct atgtcttcga ggacagtgat tacgagaaaa cgcttgaatt gatcgacaca 2280 acttccatct acggccttac tggagcaatc gtgagtaccg aacatgtgcc ctgcttattg 2340 tttagatgtt catatccgta tcagttcgct tctgaacgtc aagctttact taccgccaca 2400 aaccggtctc gcaatgcggc tggtaacata tattacaacg aaaagtgcac gggagcagtt 2460 gtcggtcagc aaccgttcgg tggcgccaga gggagtggca ccaacgacaa ggccggtagc 2520 attagtatct tctatcgctt tgttagcgcg aggagcatca aggagaactt tgtcgggttg 2580 gaagacttcc actatccctc taatttggtt tgagagcatg ttgacgatgg acttttggtg 2640 tacaaacagt tgttttagta atgtagtgac atatctccaa attgcttatc agagatccag 2700 tccgtcatca gcgcggagaa ggtggatgaa gctcatataa acgtataaat agatgttctc 2760 cgataatgta gcgtgggaca gccgtggtgg ctatacagcg ttgcgtcgcc gcccccgctc 2820 atttgagtca cctcccactc tctcttccgc aaccaccacc atggctcaga aaacaccccg 2880 ttagcagtcg ccgtcaaggt tccccaatat gcccgggaaa acggcggatt tggaccagac 2940 ttgggcctac cttactagtg gcgtcgac 2968 // |
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