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EBI DbfetchID X91877; SV 1; linear; genomic DNA; STD; PRO; 7574 BP. XX AC X91877; XX DT 18-APR-1996 (Rel. 47, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 8) XX DE A.eutrophus odhA, odhB, odhL, ORF1 and ORF5 genes XX KW 2-oxoglutarate dehydrogenase; dihydrolipoamide dehydrogenase; KW dihydrolipoamide succinyltransferase; odhA gene; odhB gene; odhL gene. XX OS Cupriavidus necator OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; OC Burkholderiaceae; Cupriavidus. XX RN [1] RP 1-7574 RA Hein S.; RT ; RL Submitted (29-SEP-1995) to the EMBL/GenBank/DDBJ databases. RL S. Hein, Inst. f. Mikrobiologie, Corrensstrasse 3, D- 48149 Muenster, FRG XX RN [3] RP 1-7574 RX DOI; 10.1016/0378-1097(95)00487-4 RX PUBMED; 8867378. RA Hein S., Steinbuechel A.; RT "Cloning and characterization of the Alcaligenes eutrophus 2-oxoglutarate RT dehydrogenase complex"; RL FEMS Microbiol. Lett. 136(3):231-238(1996). XX DR RFAM; RF01070; sucA. XX FH Key Location/Qualifiers FH FT source 1..7574 FT /organism="Cupriavidus necator" FT /strain="H16" FT /strain="DSM 428" FT /strain="ATCC 17699" FT /mol_type="genomic DNA" FT /db_xref="taxon:106590" FT RBS 119..122 FT /note="putative" FT CDS 129..875 FT /transl_table=11 FT /gene="orf1" FT /db_xref="GOA:Q44028" FT /db_xref="InterPro:IPR004129" FT /db_xref="UniProtKB/TrEMBL:Q44028" FT /protein_id="CAA62979.1" FT /translation="MTSVTDTAWRYPSHIAHRGAGKLAPENTLAAFRHGAGFGYRMFEF FT DVKLSADGKPVLMHDATLDRTTSGQGRVDALTLGELAQLDAGTWHSPAFAGEPVPTLAA FT IARYTQANGLMVNIEIKPVPGTEARTGAAVALDAQSLWAAAAVPPLLSSFSEEALAAAA FT RVAPDLPRALLLDQLPADWLERLRRLGCVALDANHRELDAGVIAAAHAAGFRVLCYTVN FT DPARAAQLLAWGLDGLITDAVDQIAP" FT -10_signal 983..988 FT /note="putative" FT -35_signal 962..967 FT /note="putative" FT RBS 1186..1189 FT /note="putative" FT RBS 1194..1197 FT /note="putative" FT CDS 1205..4057 FT /transl_table=11 FT /gene="odhA" FT /product="oxoglutarate dehydrogenase (E1)" FT /EC_number="1.2.4.2" FT /db_xref="GOA:Q59106" FT /db_xref="InterPro:IPR005475" FT /db_xref="UniProtKB/Swiss-Prot:Q59106" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA62980.1" FT /translation="MMQQYQSNSYLFGGNAPYVEELYEAYLQNPASVPDNWRAYFDAMQ FT NVPAVDGSNGRDIPHAPIVASFAERAKQGPIRTIVASADSDMGRKRVAATQLIAAYRNI FT GSHWADLDPLKRQERPPLPDLDPAFYGFSEADLDIVFNASNTYFGKESMSLRELLNNLR FT ETYCGTIGFEFMYVSDQAQKRWWQERLETTRSKPVFTLEKKKHILDRLTAAEGLERFLH FT TKYVGQKRFSLEGGESFIAAMDELIQHAGSKGVQEIVIGMAHRGRLNVLVNTLGKMPAD FT LFAEFEGKHVDDLPAGDVKYHKGFSSDVSTEGGPVHLSLAFNPSHLEIVNPVVEGSAKA FT RQERRGEVGHKEVLPVQVHGDAAFAGQGVVMETLNLAQTRGYGTGGSMHIVINNQIGFT FT TSDPRDARSTLYCTDVVKMIEAPVLHVNGDDPEAVVYAMQLAVDFRMEFKKDVVVDIIC FT FRKLGHNEQDTPAVTQPLMYKKIAQHPGTRKLYADKLAAQNLVPAEFGDEKVKAYRAAM FT DAGKHTADPVLSNFKNKFAVDWMPFLNRKWTDAADTAVPVTELKRLAERITTTPETLKL FT HPLVEKVVKDRANMGRGDQPLDWGMGEHLAFASLVSSGYPVRITAQDAGRGTFTHRHAV FT LHDQARERWDAGSYVPLQNVSENQAPFTVIDSVLSEEAVLGFEYGYSAAEPNALVIWEA FT QFGDFVNGAQVVIDQFISSGEVKWGRASGLTLMLPHGYEGQGPEHSSARIERFLQLCAD FT HNMQVCQPTTPAQIFHLLRRQMIRLFRKPLVIMTPKSLLRNKDAVSPLSDLAKGHFETV FT IPDHEELNASKVKRVIMCSGKVYYDLVNTRKEREANDTAVIRLEQLYPFPHKAVAAELK FT KYPNATEIVWCQDEPQNQGAWFFVQHYIMENMTDGQKLGYAGRPASASPAVGYYAKHNE FT QQKALLEAAFAKLKGFVLTK" FT RBS 4133..4136 FT /note="putative" FT CDS 4147..5397 FT /transl_table=11 FT /gene="odhB" FT /product="dihydrolipoamide S-succinyltransferase (E2)" FT /EC_number="2.3.1.61" FT /db_xref="GOA:P52993" FT /db_xref="HSSP:1C4T" FT /db_xref="InterPro:IPR006255" FT /db_xref="UniProtKB/Swiss-Prot:P52993" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA62981.1" FT /translation="MAIVDVKVPQLSESVAEATMLNWKKKPGEAVAQDEILIEIETDKV FT VLEVPAPSAGVLSIIVKNDGDTVVADEIIAKIDTEATAGAAAPAAAAPAPAAAAPAPAA FT AVAAPAAAGGVAMPSAAKLMAEAGLSAGQVAGTGKDGRITKGDALAAAAAPAAKAAPAP FT AAAKPALQQVSAPVDFAALGDRPEERVPMSRLRARIAERLLQSQSTNAILTTFNEVNMK FT PVMDLRNKYKDRFEKEHGVKLGFMSFFVKAAVHALKKFPLINASIDGNDIVYHGYFDIG FT IAVGSPRGLVVPILRNADQMSLADIEKKIAEFGVKARDGKLSLEELTGGTFSISNGGVF FT GSMLSTPIINPPQSAILGVHATKDRPVVEDGQIVIRPMNYLAMSYDHRIIDGREAVLGL FT VAMKDALEDPARLLLDL" FT RBS 5489..5493 FT /note="putative" FT CDS 5500..6924 FT /transl_table=11 FT /gene="odhL" FT /product="dihydrolipoamide dehydrogenase (E3)" FT /EC_number="1.8.1.4" FT /db_xref="GOA:P52992" FT /db_xref="HSSP:3LAD" FT /db_xref="InterPro:IPR001327" FT /db_xref="UniProtKB/Swiss-Prot:P52992" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA62982.1" FT /translation="MSKQFDVLVIGAGPGGYIAAIRAGQLGLNVACCEGNPYDDPKGEA FT RLGGTCLNVGCIPSKALLASSEEFENVQHHLGDHGITVGDVKVDVAKMLKRKDDIVGKM FT TKGIEFLFRKNKVTLLKGYGKFVGKSAEGFQVDVAGEVVTAKQVIIATGSKARHLPGIK FT VDNDLVSDNEGALKFPAVPKKLGVIGAGVIGLELGSVWRRLGSDVTVLEALPAFLGAAD FT EGVAKEAQKQLTKQGLKFSLGVNVNEVTTGKNGVTVKYTDKDGKAQTLEVDRLIVSVGR FT VPNTDNLGLDAVGLAADQRGFIEVDDHCATKVPGLWAIGDVVRGPMLAHKAEDEGVAVA FT ERIAGQKPHIDYNCVPWVIYTFPEIAWVGKTEAQLKAEGREYKAGQFPFMANGRALGMG FT HADGFVKMLADAKTDEILGVHIVAANASDLIAEAVVAMEFKAASEDIGRVCHPHPSMSE FT VMREAALAVDKRHVNM" FT RBS 7001..7004 FT /note="putative" FT CDS 7015..>7574 FT /transl_table=11 FT /gene="orf5" FT /db_xref="GOA:Q44029" FT /db_xref="InterPro:IPR005654" FT /db_xref="UniProtKB/TrEMBL:Q44029" FT /protein_id="CAA62983.1" FT /translation="MNVTEYYEKELKERGYQSDEAQLRAVARLQQCYDEWVAYKSRRNN FT ALKKLLVRPDVPKGVYLWGGVGRGKSFLMDSFYTCVPVVRKTRLHFHEFMREVHRQLEE FT LRGRPDPLDELARRIARRFRLICFDEFHVSDVADAMILHRLLQQMFENGVQFVMTSNYR FT PDLLYPDGLHRDRVLPAIALLQR" XX SQ Sequence 7574 BP; 1428 A; 2490 C; 2429 G; 1227 T; 0 other; acaggcggtg gtggtgcgcc tggacagcca tgcgcacgtg cgcgccggcg accgcctgcc 60 ggtgacgtcg tcggcggagc acctgcattt ctttgcgccc gatacgggca agcgcatcga 120 ggcctgacat gaccagcgta accgacactg cctggcggta cccgagccat atcgcgcacc 180 gcggcgccgg caagctggcg ccggagaaca cgctggcagc gtttcgccac ggcgccggct 240 ttggctaccg gatgttcgag ttcgacgtga agctgtcggc cgacggcaag ccggtgctga 300 tgcacgacgc cacgctggac cgcaccacca gcggccaggg cagggtggat gcgctgacac 360 tgggcgaact cgcgcagctg gacgccggca cgtggcacag ccccgccttc gccggcgagc 420 ccgtgccgac gctggcggcc atcgcacgct atacccaggc caacggcttg atggtcaata 480 tcgagatcaa gccagtgccc ggcaccgagg cgcgcaccgg tgcggcagtg gcgctggacg 540 cgcagtcgct gtgggcggct gccgcggtgc cgccgttgct gtcatcgttt tctgaagagg 600 cgctggccgc cgcggcgcgc gtcgcgccgg acctgccgcg cgcgctgctg ctcgaccagc 660 tgcccgcgga ctggctggag cggctgcgcc ggctgggctg cgtggcgctg gatgccaacc 720 accgcgaact ggatgccggc gtgatcgccg ccgcgcacgc ggccggtttc cgggtcctct 780 gctataccgt gaacgatccg gcgcgggcgg cgcagttgct ggcctggggc ctggacgggc 840 tgatcacaga cgccgtcgac cagatcgcgc cttgattcaa attgttgtgc gggtgttgct 900 agttcgctga ttgcgaaccg aattgtcgtg ggtaggtgtt tcgtatcgcg aaaagtccac 960 attgatcaaa ggcaattcat gctattctta acgccgtgaa gactttaggt ccttccccgc 1020 acaacttgcc ctttcgggcc ttgtgaggaa gccatcacac gcggcaccca agcggtgccg 1080 tttgtgtagg cgcctcagtt gggctcccaa gagttgtttg cgatccgcta accggtcaag 1140 ccgtgtcgcg gaaggttgaa taacccgctg aactccggca ttcccggaga atagtgagcg 1200 tcccatgatg cagcagtatc agagcaactc gtacctcttc ggcggtaacg ccccgtatgt 1260 cgaagaactg tacgaggcct acctccagaa ccccgcctcg gttcccgaca attggcgcgc 1320 gtatttcgac gccatgcaga acgttcctgc ggtcgacggc tcgaacggcc gggatatccc 1380 ccacgccccc atcgttgcct cgttcgccga gcgcgccaag caaggcccca tcaggaccat 1440 cgttgcctcg gccgattccg acatgggccg caagcgcgtc gctgctaccc agctgatcgc 1500 cgcctaccgc aacatcggtt cgcactgggc cgacctggat ccgctcaagc gccaggagcg 1560 tccgcccctg ccggatctgg acccggcgtt ctacggtttc tccgaagcag acctcgacat 1620 cgtcttcaat gccagcaata cctactttgg caaggagtcg atgagcctgc gcgagctgct 1680 caacaacctg cgcgaaacct actgcggcac catcggtttc gaattcatgt acgtgagcga 1740 ccaggcgcag aagcgctggt ggcaggagcg cctggaaacc acgcgttcca agcccgtgtt 1800 cacgctggaa aagaagaagc acatcctgga ccgcctgacc gcggctgaag gcctggagcg 1860 cttcctgcac accaagtacg tcggccagaa gcgcttctcg ctggaaggcg gcgaaagctt 1920 catcgccgcc atggacgagc tgatccagca cgccggcagc aagggcgtgc aggaaatcgt 1980 gatcggcatg gcccaccgtg gccgcctgaa cgtgctggtc aacaccctgg gcaagatgcc 2040 cgccgacctg ttcgccgaat tcgaaggcaa gcacgtcgac gacctgccgg ccggtgacgt 2100 caagtaccac aagggcttct cgagcgatgt ctcgaccgaa ggcggcccgg tccacctgtc 2160 gctggcgttc aatccgtcgc acctggaaat cgtcaacccg gtggtcgaag gctcggccaa 2220 ggcccgccag gaacgccgcg gcgaagtcgg ccacaaggaa gtgctgccgg tgcaggtgca 2280 cggcgatgcg gcctttgccg gccagggcgt ggtgatggag acgctgaacc tcgcgcagac 2340 ccgcggctac ggcacgggcg gctcgatgca catcgtcatc aacaaccaga tcggcttcac 2400 tacctccgac ccgcgcgacg cgcgttcgac gctgtactgc acggacgtgg tcaagatgat 2460 cgaggccccg gtgctgcacg tgaacggcga cgatcccgaa gccgtggtgt acgccatgca 2520 gctggccgtg gacttccgca tggagttcaa gaaggatgtc gtggtcgaca tcatctgctt 2580 ccgcaagctg ggccacaacg agcaggacac cccggcggtc acgcagccgc tgatgtacaa 2640 gaagatcgcc cagcaccccg gcacgcgcaa gctgtacgct gacaagctgg ccgcgcagaa 2700 cctggtcccg gccgagttcg gcgacgagaa ggtcaaggca taccgcgccg cgatggacgc 2760 cggcaagcac accgccgacc ccgtcctgtc gaacttcaag aacaagttcg ccgtggactg 2820 gatgccgttc ctgaaccgca agtggaccga cgccgccgac accgccgtgc cggtgaccga 2880 gctcaagcgc ctggccgaac gcatcaccac cacgcctgag accctgaagc tgcacccgct 2940 ggtggagaag gtcgtcaagg accgcgccaa catgggccgc ggcgaccagc cgctggactg 3000 gggcatgggt gagcacctgg ccttcgcctc gctggtgtcg tcgggctacc cggtgcgtat 3060 caccgcccag gacgctggcc gcggcacctt cacccaccgc cacgccgtgc tgcacgacca 3120 ggcccgcgag cgctgggatg ccggcagcta cgtgccgctg cagaacgtgt cggaaaacca 3180 ggcaccgttc acggtgatcg actcggtgct gtccgaagag gccgtgctcg gcttcgaata 3240 cggctactcg gccgccgaac cgaacgcgct ggtgatctgg gaagcccagt tcggcgactt 3300 cgtcaacggc gcccaggtgg tgatcgacca gttcatctcg tcgggtgaag taaagtgggg 3360 ccgtgcctcg ggcctgacgc tgatgctgcc gcacggctac gaaggccagg gcccggaaca 3420 cagctcggcc cgtatcgagc gcttcctgca gctttgcgcg gaccacaaca tgcaggtctg 3480 ccagccgacc acgccggccc agatcttcca cctgctgcgc cgacagatga tccgtctgtt 3540 ccgcaagccg ctggtgatca tgacgccgaa gtcgctgctg cgtaacaagg acgctgtgtc 3600 gccgctgtcg gacctggcca agggccactt cgagacggtc atccctgacc acgaagagct 3660 caacgccagc aaggtcaagc gcgtcatcat gtgctcgggc aaggtctact acgacctggt 3720 caacacgcgc aaggaacgcg aggccaatga tacggcggtg atccgcctgg aacagctgta 3780 tccgttcccg cacaaggctg tggcagcaga actcaagaag tatccgaacg ccaccgaaat 3840 cgtgtggtgc caggacgagc cgcagaacca gggtgcctgg ttcttcgtgc agcactacat 3900 catggaaaac atgacggatg gccagaagct cggttatgcc ggtcgtcccg cttcggcttc 3960 gccggcagtg ggctactacg caaagcacaa cgagcaacag aaggcgctgc tggaggctgc 4020 cttcgccaag ctcaagggct ttgttctgac caagtaatca agcggcagct acggcggcgc 4080 gcgcagacgg ccgccgtggc atagccagca taaagacaac acgcataaca cgaggaattc 4140 acaaccatgg ctatcgttga cgtcaaggtt ccgcagctct ccgaatcggt cgccgaagcg 4200 accatgctca actggaagaa gaagccgggc gaggctgttg cacaggacga aatcctgatc 4260 gagatcgaaa ccgacaaggt cgtgctggaa gtgccggccc cgtcggctgg tgtcctgtcg 4320 attatcgtca agaacgacgg cgacaccgtc gtggccgatg agatcatcgc caagatcgac 4380 accgaagcca ccgctggcgc cgccgcaccg gctgctgccg ctccggcacc tgccgccgct 4440 gccccggcac ctgccgctgc tgtcgccgct ccggccgccg caggtggtgt ggcgatgccg 4500 tcggctgcca agctgatggc agaagccggc ctgtcggctg gccaggttgc aggcaccggc 4560 aaggacggcc gcatcaccaa gggtgacgcg ctggccgctg ctgctgcccc ggccgccaag 4620 gctgctccgg ctccggctgc cgccaagccg gcgctgcagc aggtttcggc cccggttgac 4680 tttgccgcgc tgggcgaccg cccggaagag cgcgtgccga tgagccgtct gcgcgcccgt 4740 atcgccgagc gcctgctgca atcgcaatcg accaacgcca tcctcaccac cttcaatgaa 4800 gtgaacatga agccggtgat ggacctgcgc aacaagtaca aggaccgctt cgagaaggaa 4860 cacggcgtca agctgggctt catgtcgttc ttcgtcaagg ccgcggtgca cgcgctgaag 4920 aagttcccgc tgatcaatgc ttcgatcgac ggcaacgaca ttgtctacca cggctacttc 4980 gacatcggca tcgccgtggg ttcgccgcgt ggcctggtgg tgcccatcct gcgcaatgcc 5040 gaccagatga gcctggccga catcgagaag aagattgccg agttcggcgt caaggcccgt 5100 gacggcaagc tgtcgctgga agaactgacc ggcggtacct tctcgatctc caacggcggc 5160 gtgttcggtt cgatgctgtc gaccccgatc atcaacccgc cgcaatcggc catcctgggc 5220 gtgcacgcca ccaaggaccg cccggtggtg gaagacggcc agatcgtgat ccgcccgatg 5280 aactacctgg cgatgtctta tgaccaccgc atcatcgacg gccgtgaagc cgtgctgggc 5340 ctggtggcca tgaaggacgc gctggaagat ccggcccgcc tgctgctgga cctgtaatta 5400 acggtccccc cgcgcacgcc tgccgtgcgc ggggcgcgct tcccccgtga tgaaggcgcg 5460 ccggcacaag ctgtcgccgg cgcccacaaa ggaatttcca tgagcaaaca attcgacgtg 5520 ctggtgatcg gcgccggccc cggcggctac atcgccgcca tccgcgccgg ccagctgggc 5580 ctgaacgtgg cctgctgcga aggcaatccg tatgacgatc ccaagggcga agcccgcctg 5640 ggcggcacct gtctgaacgt gggctgcatc ccctccaagg cgctgctggc ttcctcggag 5700 gagttcgaga acgtccagca ccacctgggc gaccacggca tcaccgtggg cgacgtcaag 5760 gtcgacgtgg ccaagatgct caagcgcaag gacgacatcg ttggcaagat gaccaagggt 5820 atcgagttcc tgttccgcaa gaacaaggtg acgctgctca agggctacgg caagttcgtc 5880 ggcaagagcg ccgagggctt ccaggtcgac gttgccggtg aagtcgtgac cgccaagcag 5940 gtcatcatcg ccaccggttc gaaggctcgc cacctgccgg gcatcaaggt cgacaatgac 6000 ctggtcagcg ataacgaggg cgcgctcaag ttccccgcag tgccgaagaa gctgggcgtg 6060 atcggtgccg gcgtgatcgg cctggaactg ggctcggtgt ggcgccgcct gggctccgat 6120 gtgaccgtgc tggaagcgct gcccgcgttc ctgggcgccg ccgacgaagg cgtggccaag 6180 gaagcgcaga agcagctgac caagcagggc ctgaagttca gcctcggcgt gaacgtcaac 6240 gaagtgacga ccggcaagaa cggcgtgacc gtcaagtaca ctgacaagga cggcaaggcg 6300 cagaccctgg aggtcgatcg cctgatcgtg tcggttggcc gcgtgcccaa taccgacaac 6360 ctgggcctgg acgccgtcgg cctggccgcg gaccagcgcg gcttcatcga ggtggacgac 6420 cactgcgcca ccaaggtgcc gggcctgtgg gccatcggcg acgtggtgcg cggcccgatg 6480 ctggcgcaca aggccgagga cgaaggcgtg gccgtggccg agcgcatcgc cggccagaag 6540 ccgcatatcg actacaactg cgttccgtgg gtgatctaca ccttcccgga aatcgcatgg 6600 gtaggcaaga ccgaagccca gctcaaggcc gaaggccgcg agtacaaggc cggccagttc 6660 ccgttcatgg ccaacggccg tgcactgggc atgggccacg ccgacggctt cgtcaagatg 6720 ctggccgacg ccaagaccga cgagatcctg ggcgtgcaca tcgttgccgc caacgcctcg 6780 gacctgatcg ccgaagccgt ggtggcgatg gagttcaagg ccgccagcga ggatatcggc 6840 cgcgtctgcc acccgcaccc gtcgatgtcg gaagtcatgc gcgaagccgc gctggccgtc 6900 gacaagcgtc acgttaatat gtaaggcgca tgcttcgcat tgcctgagta ccggcgcgcc 6960 gcatccgcgg cgcgccgttt tcacttgcgg cccgtgccgc aggaacccgt cagcatgaac 7020 gtcaccgagt actacgagaa ggaactgaag gagcgcggct accagtccga cgaagcgcaa 7080 ttgcgtgccg tggcccggct gcagcagtgc tacgacgaat gggtggccta caagagccgc 7140 cgcaacaatg cgctgaagaa gctgctggtc cgtcccgacg tgcccaaggg cgtctacctg 7200 tggggtgggg tggggcgcgg caagtccttc ctgatggaca gcttctatac ctgcgtgccg 7260 gtggtgcgca agacgcggct gcatttccat gagttcatgc gcgaggtcca tcgccagctc 7320 gaagagctgc gcggccgtcc cgatccgctg gatgagctgg ccagacgcat cgcgcgccgc 7380 ttccggctga tctgcttcga tgaattccac gtcagcgacg tcgccgacgc gatgatcctg 7440 catcggctgc tgcagcagat gttcgagaac ggcgtgcagt tcgtgatgac ctccaactac 7500 cgcccggacc tgctgtatcc ggatggcctg caccgcgacc gcgtgctgcc ggccatcgcg 7560 ctgctgcagc ggaa 7574 // |
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