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EBI DbfetchID X89810; SV 1; linear; genomic DNA; STD; PRO; 6811 BP. XX AC X89810; XX DT 27-MAR-1997 (Rel. 51, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 6) XX DE B.subtilis DNA for araABDLMNPQ-abfA operon XX KW abfA gene; alpha-L-arabinofuranosidase; araL gene; araM gene; araN gene; KW araP gene; araQ gene; integral membrane protein; sugar-binding protein; KW terminator. XX OS Bacillus subtilis OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RX PUBMED; 9084180. RA Sa-Nogueira I., Nogueira T.V., Soares S., Lencastre H.; RT "The Bacillus subtilis L-Arabinose (ara) operon: Nucleotide Sequence, RT Genetic Organization and Expression"; RL Microbiology (Reading, Engl.) 143:957-969(1997). XX RN [2] RP 1-6811 RA Sa-Nogueira I.; RT ; RL Submitted (19-JUL-1995) to the EMBL/GenBank/DDBJ databases. RL I. Sa-Nogueira, Instituto de Teconologia Quimica e Biologica, Rua da Quinta RL Grande n. 6, Apt 127, P- 2780 Oeiras Codex, PORTUGAL XX CC Related sequence X89408 XX FH Key Location/Qualifiers FH FT source 1..6811 FT /organism="Bacillus subtilis" FT /map="256 degrees" FT /strain="168T+" FT /mol_type="genomic DNA" FT /db_xref="taxon:1423" FT CDS 1..810 FT /transl_table=11 FT /gene="araL" FT /product="AraL protein" FT /function="unknown" FT /db_xref="GOA:P94526" FT /db_xref="InterPro:IPR006357" FT /db_xref="UniProtKB/Swiss-Prot:P94526" FT /protein_id="CAA61932.1" FT /translation="MASHDTPVSPAGILIDLDGTVFRGNELIEGAREAIKTLRRMGKKI FT VFLSNRGNISRAMCRKKLLGAGIETDVNDIVLSSSVTAAFLKKHYRFSKVWVLGEQGLV FT DELRLAGVQNASEPKEADWLVISLHETLTYDDLNQAFQAAAGGARIIATNKDRSFPNED FT GNAIDVAGMIGAIETSAQAKTELVVGKPSWLMAEAACTAMGLSAHECMIIGDSIESDIA FT MGKLYGMKSALVLTGSAKQGEQRLYTPDYVLDSIKDVTKLAEEGILI" FT RBS 791..796 FT /gene="araM" FT CDS 807..1991 FT /transl_table=11 FT /gene="araM" FT /product="AraM protein" FT /function="unknown" FT /db_xref="GOA:P94527" FT /db_xref="InterPro:IPR002658" FT /db_xref="UniProtKB/Swiss-Prot:P94527" FT /protein_id="CAA61933.1" FT /translation="MNRIAADVQRAFENAGEKTLPIKVEEIVLGKQAADSLLDYVKRKN FT NQHIVLVCDANTHRIAGIDLENRLNQEGFQAECLIIPENEAGDVTADERSLIHVLIHTK FT QPTDVMIAVGSGTIHDIVRFAAFQRDLPFISYPTAPSVDGFTSAGAPIILYGTKTTIQT FT KAPSALFADLDLLKAAPQSMVAAGFGDMLGKITSLADWEISRHLAGEPYSPAGAKIVQE FT ALAACIEHTEDIAMKTETGIRVLMESLLVSGLVMLALDHSRPASGGEHHISHWIEMELM FT EKKRPQILHGAKVGCAAVLLTDTYRKLAQDDGLNEFSPSRREAIQSAYQTLPRGEVLAD FT WLRSAGGPADFDEIGVGQDSVKNAFRHAHTLRDRCTGLRIINENKTLINHGLYE" FT RBS 2008..2010 FT /gene="araN" FT CDS 2022..3323 FT /transl_table=11 FT /gene="araN" FT /product="putative sugar-binding protein" FT /function="component of a high affinity transport system FT for L-arabinose and/or L-arabinose oligomers" FT /db_xref="GOA:P94528" FT /db_xref="InterPro:IPR006061" FT /db_xref="UniProtKB/Swiss-Prot:P94528" FT /protein_id="CAA61934.1" FT /translation="MKKMTVCFLVLMMLLTLVIAGCSAEKSSGKSGETELTFWTFNGLH FT EQFYVEMVKEWNKKYPDRKIKLNTVVYPYGQMHDNLSISLIAGEGVPDIADVELARFSN FT FLKGSDIPLADLTPLIEKDRDKFVEARLTLYSKNGKLYGLDTHVGTTVMFYNMDVMKKA FT GVNPDDIKTWDDYHKAGQKVRKVTGKPMGTVETNDSATFLSMISQQNSGYFDKNGKLIL FT NNDTNVKTLQYLKDMINDKTMIPAPGGGHHSEEYYGFMNQGGAASVLMPIWYMGRFIDY FT MPDLKGKIAIRPLPAWKEGGDRSAGLGGTATVVPKQSKHVELAKEFLAFAKGSEEGNKK FT LWSVLGFDPLRWDVWSSKELKEKNKYTDYFQNGTGIFSVLLDIKDEINPIYLHEDFAKA FT SDLVNRSVLFDALKSQQKTPKQALDRAAGELKQK" FT RBS 3349..3351 FT /gene="araP" FT CDS 3359..4300 FT /transl_table=11 FT /gene="araP" FT /product="putative integral membrane protein" FT /function="component of a high affinity transport system FT for L-arabinose and/or L-arabinose oligomers" FT /db_xref="GOA:P94529" FT /db_xref="InterPro:IPR000515" FT /db_xref="UniProtKB/Swiss-Prot:P94529" FT /protein_id="CAA61935.1" FT /translation="MKPVKTGTVHPVPSAAKQSGWRDLFYSKKAAPYLFTAPFVLSFLV FT FFLYPIISVFIMSFQRILPGEVSFVGLSNYTALNNPTFYTALWNTLEYTFWTLIVLIPV FT PLLLAIFLNSKLVKFRNIFKSALFIPALTSTIVAGIIFRLIFGEMETSLANSILLKLGF FT SPQNWMNNEHTGMFLMVLLASWKWMGINILYFLAGLQNVPKELYEAADIDGANTMKKFL FT HITLPFLKPVTVYVLTISIIGGFRMFEESYVLWQNNSPGNIGLTLVGYLYQQGLAYNEM FT GYGAAIGIVLLIVILVVSLISLKLSGSFKGEG" FT RBS 4286..4288 FT /gene="araQ" FT CDS 4301..5146 FT /transl_table=11 FT /gene="araQ" FT /product="putative integral protein" FT /function="component of a high affinity transport system FT for L-arabinose and/or L-arabinose oligomers" FT /db_xref="GOA:P94530" FT /db_xref="InterPro:IPR000515" FT /db_xref="UniProtKB/Swiss-Prot:P94530" FT /protein_id="CAA61936.1" FT /translation="MLRHSPQFSVYRIALTLFFMMLSLLYLFPIFCLLLGSLKPSSELL FT RVGLNLDIDPKVMSFDNYTFLFNGGSIYFKWFFNSLVLGLFTTVLTLFFSSMIGYGLAV FT YDFKGRNIIFVLVLIIMMVPLEVMMLPLFKLTVGLHLIDSYTGVILPFIVSPVAVFFFR FT QYALGLPRDLLDSARMDGCTEFGIFFRIMAPLMKPAFGAMIILQSLNSWNNFLWPLIVL FT RSKEMFTLPIGLSSLLSPYGNNYDMLISGSVFAILPVIIIFLFFQKYFISGLTVGGVKG FT " FT RBS 5149..5152 FT /gene="abfA" FT CDS 5165..6667 FT /transl_table=11 FT /gene="abfA" FT /product="putative alpha-L-arabinofuranosidase" FT /db_xref="GOA:P94531" FT /db_xref="InterPro:IPR010720" FT /db_xref="UniProtKB/Swiss-Prot:P94531" FT /protein_id="CAA61937.1" FT /translation="MKKARMIVDKEYKIGEVDKRIYGSFIEHMGRAVYEGIYEPDHPEA FT DEDGFRKDVQSLIKELQVPIIRYPGGNFLSGYNWEDGVGPVENRPRRLDLAWQTTETNE FT VGTNEFLSWAKKVNTEVNMAVNLGTRGIDAARNLVEYCNHPKGSYWSDLRRSHGYEQPY FT GIKTWCLGNEMDGPWQIGHKTADEYGRLAAETAKVMKWVDPSIELVACGSSNSGMPTFI FT DWEAKVLEHTYEHVDYISLHTYYGNRDNNLPNYLARSMDLDHFIKSVAATCDYVKAKTR FT SKKTINLSLDEWNVWYHSNEADKKVEPWITARPILEDIYNFEDALLVGSLLITMLQHAD FT RVKIACLAQLVNVIAPIMTEKGGEAWRQPIFYPYMHASVYGRGESLKPLISSPKYDCSD FT FTDVPYVDAAVVYSEEEETLTIFAVNKAEDQMETEISLRGFESYQIAEHIVLEHQDIKA FT TNQHNRKNVVPHSNGSSSVSENGLTAHFTPLSWNVIRLKKQS" FT terminator 6668..6705 FT /note="putative" FT terminator 6718..6737 FT /note="putative" XX SQ Sequence 6811 BP; 1884 A; 1435 C; 1679 G; 1813 T; 0 other; atggccagtc atgatacgcc tgtgtcaccg gctggcattc tgattgactt ggacggtact 60 gtattcagag gaaatgagtt gatcgaagga gcaagagaag cgatcaaaac gcttaggaga 120 atgggaaaga aaatcgtctt tttaagcaac cgggggaata tctcccgtgc catgtgcaga 180 aaaaaacttc ttggcgcggg gattgaaacg gacgtaaacg acattgttct gtcatcaagc 240 gtgacagcgg cttttctgaa aaaacattat cgtttttcaa aggtatgggt gcttggggag 300 caaggcttgg ttgacgagct gaggctggcc ggtgtgcaga acgcgagcga accgaaggaa 360 gcggattggc tcgtgatctc ccttcatgaa acgctcacgt acgacgattt aaatcaagcc 420 tttcaagcgg ctgccggcgg cgctcgtatt atcgctacaa acaaagaccg ctcttttccg 480 aacgaagacg gaaatgccat tgatgtggcc ggaatgatcg gggcaattga gacttctgca 540 caagcgaaga ctgaacttgt tgtcggaaaa ccgtcatggc tgatggcgga ggctgcctgt 600 acggcaatgg ggctgtccgc acatgaatgc atgattatag gagacagcat tgaatctgac 660 attgcgatgg ggaagcttta tggcatgaaa agcgccttag tgctaactgg ttctgcgaaa 720 cagggtgaac agcgtttgta cacgccggat tatgtgctgg attctattaa ggatgtaacc 780 aaattggctg aggaggggat tctgatatga atcgtatcgc agctgacgtt cagcgtgctt 840 ttgaaaacgc cggagaaaag acgttgccta taaaagttga agaaattgtt ctcggtaagc 900 aagcagctga ttcgcttttg gattatgtaa aacgaaaaaa caatcaacat attgtccttg 960 tctgcgacgc gaatacacac cgcattgcag gaattgattt agaaaaccga ctgaatcaag 1020 aaggatttca ggccgagtgc ctgatcattc cagaaaatga agccggagat gtgacagctg 1080 atgaacgatc gctcattcat gtgctgatcc atacgaaaca accaacggat gtcatgatcg 1140 cagtcggttc gggcacgatt catgatatcg tccgctttgc ggcgtttcaa agagatttgc 1200 cgtttatttc ttatccgact gctccatctg tagacggttt tacatcagcc ggtgcgccga 1260 ttattttata cggcacgaaa acaaccattc aaacgaaggc cccatctgcg ctgttcgctg 1320 atctggatct attaaaagcg gcaccgcagt caatggtggc ggctggcttt ggtgacatgc 1380 tcggtaaaat cacgtcttta gcagattggg aaatatcccg gcatcttgcc ggtgagcctt 1440 attcgcctgc aggagctaag atcgttcagg aggcgcttgc tgcctgcatt gaacacacag 1500 aagacattgc gatgaaaacg gaaactggca tacgggtttt gatggagtct ttacttgtat 1560 cggggcttgt catgctggct ttagatcatt cccgaccggc atcaggcggc gagcatcata 1620 tttcacattg gattgaaatg gagttaatgg agaaaaaacg gcctcagatt cttcatgggg 1680 caaaggtggg ctgtgccgct gttttattaa ctgacacata cagaaagctc gctcaggatg 1740 acgggctgaa cgaattttca ccaagccgcc gggaagccat ccaatcggct tatcaaacac 1800 tcccgagagg agaagtgctg gctgattggc tgagatcagc cggaggccct gctgattttg 1860 acgaaatcgg tgtcgggcag gattccgtca aaaatgcctt cagacacgcg cacaccttaa 1920 gagaccgatg caccggatta agaatcatca atgaaaacaa aacgctgatc aaccatggtc 1980 tatatgaata gcccgcacct cgaatggaag gggtaacgca gatgaaaaaa atgactgtct 2040 gttttcttgt gctcatgatg ttgctgacat tagtcattgc cgggtgttca gcagaaaaat 2100 catccggcaa atcgggtgaa actgagctga ccttttggac atttaacggg cttcatgagc 2160 agttctatgt ggaaatggtg aaggaatgga acaaaaaata tcctgaccgc aaaattaagc 2220 tgaatacggt cgtttatcca tatggacaaa tgcacgataa cttatctatc tccctaatag 2280 cgggagaagg cgttcctgat attgcagatg tcgaattggc ccgtttttca aactttttga 2340 agggctctga cataccgctt gccgacttga ctccgctgat tgaaaaggat cgcgataaat 2400 tcgttgaggc gcggctgaca ttgtacagca aaaacggaaa gctttacgga ctcgatacac 2460 atgtagggac aacggtcatg ttttataaca tggatgtgat gaaaaaagcc ggcgtcaatc 2520 ctgacgatat taaaacatgg gatgattacc ataaagccgg acagaaagtg cgcaaagtga 2580 ccgggaagcc gatgggaacg gtggaaacaa atgattccgc aacgttctta tctatgattt 2640 cacagcaaaa ctcaggctat tttgataaaa acggcaagct gatcctcaat aatgacacca 2700 acgtaaaaac acttcaatat ttaaaagaca tgatcaatga taaaacgatg attcctgcgc 2760 cgggcggcgg gcatcacagt gaagaatact acggctttat gaaccaagga ggagctgctt 2820 cagttctcat gccgatttgg tatatgggaa gatttatcga ttatatgcct gatctgaaag 2880 ggaagattgc catcagaccg ctcccggcat ggaaagaggg gggcgaccgc tcggcaggtt 2940 tgggcggtac ggcaactgtt gtaccgaagc aatccaagca tgttgagtta gcaaaagagt 3000 ttttggcctt tgcgaagggc tctgaagaag gaaataaaaa actctggagc gtactcggct 3060 ttgacccgct tcgctgggat gtttggagct ccaaggaatt gaaagagaaa aacaaataca 3120 cggattactt ccaaaacgga acaggcattt tttctgtgct gctcgatatc aaggatgaaa 3180 tcaatccaat ttatttacat gaggattttg ccaaggcttc agaccttgtc aacagaagcg 3240 tattgttcga cgcgcttaaa tctcagcaaa aaacgcctaa acaagccttg gacagagcgg 3300 caggtgaact gaaacagaaa tagaatccca ttcaaaaagt gaaagcgggg aggttctcat 3360 gaaacctgtg aaaacgggaa cggttcatcc cgttccttca gctgcgaaac aatcaggctg 3420 gcgagatctg ttttattcaa aaaaagcggc gccctatctg tttacagcgc cattcgtttt 3480 atcctttctc gtattttttc tataccccat cattagtgtc ttcatcatga gcttccaaag 3540 aattttgccg ggagaggtgt cctttgtcgg attgtctaat tatacagcgc taaacaaccc 3600 gacgttctat accgcccttt ggaatacgct ggaatacacc ttttggacgc tgatcgtgct 3660 gattcctgtt ccattgcttc tggccatatt cctgaattca aagctggtca aatttagaaa 3720 tatatttaaa tcagcattat ttatcccggc attgacctca accattgtcg cggggatcat 3780 ttttcggctg atcttcggag aaatggaaac gtctctggcc aattccatcc tacttaaact 3840 cggcttttca cctcagaact ggatgaacaa tgaacatacc ggcatgtttt tgatggtgct 3900 gcttgcttca tggaaatgga tgggaatcaa catcctttac tttttagcag gtttgcaaaa 3960 tgtgccgaaa gagctgtacg aagccgctga tatagacggc gcgaatacaa tgaaaaaatt 4020 tctgcacatc acgctgccgt ttctcaagcc tgtaaccgta tatgtgctga ccatcagcat 4080 catcggcggc ttcaggatgt ttgaggaaag ctacgtcctt tggcagaata attccccggg 4140 taatattggt ctgacgcttg tcggatattt gtatcagcag ggacttgcct acaatgaaat 4200 gggatacgga gcggccatcg gcattgtgct tttgattgtg atccttgttg tcagcctgat 4260 ttcattaaag ctgtcaggct cgtttaaggg ggagggataa atgttgcggc acagtcctca 4320 gtttagcgtt tatagaattg cgctgaccct gttttttatg atgctgagcc tattgtatct 4380 ttttccgatt ttctgtttgc ttttaggatc attaaagccg tcatctgagc ttttgcgtgt 4440 ggggctgaat cttgatattg atccaaaagt gatgagtttt gataactaca catttctgtt 4500 taatggcggc agcatttatt tcaaatggtt ttttaacagt cttgtactcg gactttttac 4560 gactgtgctc actctgtttt tttcttcgat gatcgggtac gggcttgcgg tttatgattt 4620 taagggcaga aatatcatct ttgttcttgt gctgattatt atgatggttc cgctggaagt 4680 gatgatgctt cctctgttta aacttactgt cggactgcac ttgatcgatt catatacggg 4740 tgtcatattg ccgtttatcg tttcacctgt tgctgttttc tttttcaggc aatatgctct 4800 tggccttcca agagatctgc tggactctgc aaggatggac ggctgtacgg aattcggcat 4860 ctttttcagg attatggcac cgctgatgaa accggctttc ggtgcgatga ttatccttca 4920 gtccttaaac agctggaaca acttcttgtg gccgctgatt gtgcttcggt cgaaagaaat 4980 gtttacgctt ccaatagggc tgtccagctt gctgagccct tatggaaata attacgacat 5040 gcttatatcc ggctcagtat ttgcgatttt gccggtgatt atcattttct tgtttttcca 5100 aaagtacttt atctccggcc tgacggtagg gggagtcaaa ggttaatgaa ggaggaaacg 5160 tgtgatgaaa aaagcgcgaa tgattgtaga caaagaatat aaaatcggtg aagtagataa 5220 acggatttat ggctcgttta tcgaacatat gggtcgtgcg gtatatgaag gcatatacga 5280 gcctgatcac cctgaagcgg atgaagatgg atttagaaaa gatgtccagt cgctgatcaa 5340 agaattacag gttcccatca tccgctatcc gggcggaaac tttttatccg gatacaactg 5400 ggaggacggt gtcggaccag tcgaaaaccg cccgagacgg cttgacttgg catggcaaac 5460 gacagaaacc aatgaagtgg gaacaaatga atttttatct tgggcaaaaa aggtgaacac 5520 tgaggtcaat atggccgtca accttggcac aagaggcata gatgccgccc gtaatctcgt 5580 tgaatattgc aaccatccga aaggctctta ctggagtgat ttaagaagat cgcatggcta 5640 tgaacagccg tatggcatca aaacatggtg cttaggaaac gaaatggatg gaccatggca 5700 gatcggccac aaaacagctg atgaatacgg acggcttgcc gcagagacag caaaggtcat 5760 gaagtgggtt gacccatcaa ttgaactcgt tgcctgcggc agctcaaaca gcggtatgcc 5820 gacctttatc gattgggaag cgaaggtgct tgagcatacg tatgagcatg tcgactatat 5880 ctctcttcac acttactacg gaaaccggga taacaatctg ccaaactact tggcacgttc 5940 tatggatttg gatcatttta tcaaatcagt cgctgcgacc tgtgactatg taaaagcaaa 6000 aacacgcagc aagaaaacta tcaatctctc tctggatgaa tggaacgtct ggtaccactc 6060 aaatgaggct gataaaaaag tcgagccgtg gatcactgcg cgtccgattt tagaggatat 6120 ttacaatttt gaagatgcct tattagtcgg ctctctgctc attacgatgc tgcagcacgc 6180 agaccgtgtg aaaattgcgt gtcttgcaca gcttgttaat gtcatcgcgc cgatcatgac 6240 ggaaaaaggc ggagaagcat ggagacagcc gattttctat ccatacatgc atgcttctgt 6300 ttacggaagg ggcgagtcac tgaaaccgct tatttcttct cctaagtacg attgttctga 6360 tttcactgat gtgccatatg ttgatgctgc tgttgtgtac tctgaagagg aagaaacact 6420 cactattttt gcggtaaaca aggctgagga tcagatggag acggagattt cgctcagagg 6480 ctttgaatcc taccaaatcg cagagcacat cgtacttgag catcaggata tcaaagcaac 6540 aaaccagcat aacagaaaaa atgtcgttcc gcattccaac ggatcatcgt ctgtcagcga 6600 aaacggctta actgctcatt tcacgccgct ttcctggaat gtgatccgcc tgaaaaaaca 6660 gtcataagaa tagcaaagcc ggagatttct ctccggcttg tctttcaact gccacgagcc 6720 ggcccattcc agccggcttt ttgtatagga aaaaatgacc gcttttcacc atgaaattat 6780 gatatattta tgaaaaacag aaaaggggat g 6811 // |
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