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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   X89738; SV 1; linear; genomic DNA; STD; INV; 1580 BP.
XX
AC   X89738;
XX
DT   23-JAN-1997 (Rel. 50, Created)
DT   18-APR-2005 (Rel. 83, Last updated, Version 3)
XX
DE   T.brucei DNA for gpd gene
XX
KW   glycerol-3-phosphate dehydrogenase; gpd gene.
XX
OS   Trypanosoma brucei
OC   Eukaryota; Euglenozoa; Kinetoplastida; Trypanosomatidae; Trypanosoma.
XX
RN   [1]
RX   DOI; 10.1016/0166-6851(95)02556-1
RX   PUBMED; 8920004.
RA   Kohl L., Drmota T., Thi C.D., Callens M., Van Beeumen J., Opperdoes F.R.,
RA   Michels P.A.M.;
RT   "Cloning and characterization of the NAD-linked glycerol-3-phosphate
RT   dehydrogenases of Trypanosoma brucei brucei and Leishmania mexicana
RT   mexicana and expression of the trypanosome enzyme in Escherichia coli";
RL   Mol. Biochem. Parasitol. 76(1-2):159-173(1996).
XX
RN   [2]
RP   1-1580
RA   Kohl L.;
RT   ;
RL   Submitted (17-JUL-1995) to the EMBL/GenBank/DDBJ databases.
RL   L. Kohl, International Institute of Cellular and Molecular Pathology (ICP),
RL   ICP-TROP 74.39, Avenue Hippocrate 74, B-1200 Brussels, BELGIUM
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1580
FT                   /organism="Trypanosoma brucei"
FT                   /host="mammals"
FT                   /lab_host="rat"
FT                   /sub_species="brucei"
FT                   /strain="427"
FT                   /mol_type="genomic DNA"
FT                   /clone_lib="lambda gt11"
FT                   /tissue_type="bloodstream form"
FT                   /db_xref="taxon:5691"
FT   CDS             157..1221
FT                   /gene="gpd"
FT                   /product="glycerol-3-phosphate dehydrogenase (NAD+)"
FT                   /EC_number="1.1.1.8"
FT                   /note="dimer"
FT                   /db_xref="GOA:P90593"
FT                   /db_xref="HSSP:1EVY"
FT                   /db_xref="InterPro:IPR006109"
FT                   /db_xref="UniProtKB/Swiss-Prot:P90593"
FT                   /protein_id="CAA61890.1"
FT                   /translation="MVSGVTYLKRGAVFGSGAFGTALACVLAKKCESVSVWHMNANEAR
FT                   VVNQKHENVYFLPGAPLPANLTFTADAEECAKGAEIVLFVIPTQFLRGFLQKNSHILRN
FT                   HVVSRNVPVVMCSKGIERSSLLFPAQILEEFLPNYPIGVIAGPSFAIEVAKGMLTNVCT
FT                   AAADINMARKIQRIMTTSDGSFRCWATTDVIGCEIASAMKNVLAIASGALKGLGTENNA
FT                   RAALISRGLLEIRDLTLALGGTGEAVFGLPGLGDLLLTCSSELSRNFTVGMKLGQGISL
FT                   EEIKRTSKAVAEGVATAEPLERLAKKHNADLPICHEVYNVLYANGCAKRSFKKLNSCKL
FT                   ADEGLPALPRTSKM"
XX
SQ   Sequence 1580 BP; 348 A; 433 C; 389 G; 410 T; 0 other;
     ccatactgtt gcttcaactt cccgtctctt ttctttttct actggacgta tttgcgaaac        60
     cacggttagc aatactaaac gcctcagtta tattaatcac ccgagcaaaa agcctacagt       120
     agagcaaaga cagcaagaaa tcatatcaaa cacataatgg tatccggtgt gacatatctg       180
     aagcgcggtg cggtgttcgg cagtggcgcc ttcggtaccg ctctggcgtg cgtccttgca       240
     aagaagtgtg agtctgtgtc cgtatggcat atgaacgcca atgaggcccg tgttgtgaac       300
     cagaagcacg aaaacgtata tttccttccc ggtgcaccgc ttccagctaa ccttaccttc       360
     acggccgatg ctgaggagtg cgcgaaaggt gcggagattg tcctcttcgt catcccgacg       420
     cagttcctcc gcgggttcct ccagaagaac agccacatcc tccgcaacca cgttgtttcc       480
     cgcaacgtgc ccgtggtgat gtgcagcaag ggcatcgagc gctcctccct cctcttcccc       540
     gcacagatct tggaggagtt cctgcccaac tatccaatcg gtgttattgc tggcccttca       600
     ttcgccattg aagttgctaa gggaatgctg acaaatgttt gcactgccgc ggccgacatc       660
     aacatggcac gaaagattca gagaatcatg accacatccg acggctcctt ccgttgctgg       720
     gcaaccaccg atgttatcgg atgcgagatc gcaagcgcca tgaagaatgt gcttgccatt       780
     gcctcaggtg cactaaaggg acttggaact gagaacaatg cccgcgctgc cctcatctct       840
     cgtggtcttc tcgaaatccg tgacctgacc ctggcacttg gcggcacggg tgaggctgtc       900
     ttcggccttc ccggcctcgg cgacttactg ctcacctgct cttcagaact ctcacgtaac       960
     tttacggtgg gcatgaagct cggccaaggc atttccctcg aagagatcaa gcgcaccagc      1020
     aaggccgttg ctgagggtgt tgcaaccgct gaaccgctgg agcgactcgc caagaagcac      1080
     aacgctgacc tccccatatg ccatgaggtc tacaatgtgt tgtatgccaa cggttgtgcc      1140
     aaacgctcgt tcaagaagct caactcctgc aagttggctg atgaggggct cccggcgctc      1200
     ccgcgcactt ccaaaatgta aaacacgatt acattcgtgt gggtggtgtg ggtttagcaa      1260
     agtggacgtg ggtttccttt cttgttatat gccatatttc aataacaaca atagggagta      1320
     agcggcagtg cggctccgag gttgtggcca tattgcgttc cttcctcatt atgcggcatc      1380
     taacggttga atctaaggtt tcttcaattt ttgcccttct gaatcttttc tttttcgttt      1440
     ctcattaatt ttgattttga tccaacaatt ctaaaacgtg aatagcctta taggcctgat      1500
     gctggaaact ttcttaatgt gccaacccgt gctgtgtgtg tgtgtgtgtg tgccgctacc      1560
     cggtacccgg ctctgtgtca                                                  1580
//
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