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EBI DbfetchID X89732; SV 2; linear; genomic DNA; STD; PRO; 5595 BP. XX AC X89732; XX DT 31-DEC-1995 (Rel. 46, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 6) XX DE Clostridium thermocellum celC (partial), ORF3 and licA genes XX KW celC; endo-1,3(4)-beta-glucanase; endo-1,4-glucanase; licA; KW putative lacI-family transcriptional regulator. XX OS Clostridium thermocellum OC Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; OC Clostridium. XX RN [2] RC revised by [3] RA Schwarz W.H.; RT ; RL Submitted (14-JUL-1995) to the EMBL/GenBank/DDBJ databases. RL W.H. Schwarz, Institute for Microbiology, Technical University of Muenchen, RL Arcisstr. 21, D-80290 Muenchen, FRG XX RN [3] RP 1-5595 RA Zverlov V.; RT ; RL Submitted (08-APR-2002) to the EMBL/GenBank/DDBJ databases. RL W.H. Schwarz, Institute for Microbiology, Technical University of Muenchen, RL Arcisstr. 21, D-80290 Muenchen, FRG XX RN [4] RX DOI; 10.1099/mic.0.26153-0 RX PUBMED; 12686644. RA Fuchs K.P., Zverlov V.V., Velikodvorskaya G.A., Lottspeich F., RA Schwarz W.H.; RT "Lic16A of Clostridium thermocellum, a non-cellulosomal, highly complex RT endo-beta-1,3-glucanase bound to the outer cell surface"; RL Microbiology (Reading, Engl.) 149(Pt 4):1021-1031(2003). XX DR CABRI; DSM 1237. XX FH Key Location/Qualifiers FH FT source 1..5595 FT /organism="Clostridium thermocellum" FT /strain="DSM 1237" FT /mol_type="genomic DNA" FT /clone_lib="lambda 1059 pBR322" FT /clone="LIC7 pWS1250" FT /db_xref="taxon:1515" FT CDS <1..127 FT /codon_start=2 FT /transl_table=11 FT /gene="celC" FT /product="endo-1,4-glucanase" FT /function="endohydrolysis of 1,4-beta-D-glucosidic linkages FT in cellulose" FT /EC_number="3.2.1.4" FT /db_xref="GOA:Q8RKF1" FT /db_xref="HSSP:1CEO" FT /db_xref="UniProtKB/TrEMBL:Q8RKF1" FT /protein_id="CAD29717.1" FT /translation="LEEYDIGGAVWNYKKMDFEIYNEDRKPVSQELVNILARRKT" FT RBS 233..237 FT /note="ORF2" FT CDS 244..1281 FT /transl_table=11 FT /product="putative lacI-family transcriptional regulator" FT /note="ORF2" FT /db_xref="GOA:Q9AL35" FT /db_xref="HSSP:1PRU" FT /db_xref="InterPro:IPR000843" FT /db_xref="InterPro:IPR001761" FT /db_xref="InterPro:IPR010982" FT /db_xref="UniProtKB/TrEMBL:Q9AL35" FT /protein_id="CAD29718.1" FT /translation="MTSEEIAKLCGVSRATVSRVINNSPNVKEETRQKILAVIKEKNYV FT PIAPARRLAGIDSNIIGLFVLDIDISESKSRVSESTYFSRLINLIIDQANNFGFQVLVS FT IITSQKQLSEIRNLFMSRTIFSGIFIGAFNDEIQLDDDIIMQHPTIIIDRQSERMVKKP FT NRLVVNLDNFEGAYNATQFLIKLGHTRIGHISGDLRKLSGIERYEGYKKALEDAGLGFD FT KNLVREGNFLDDSGYRLAREILKENVTAIFCANDVMAISAIKAIKETGLSVPDDISVIG FT FDNTAIGNYIMPALTTVNAPLEHIAEACIESLKYFCEHKHFKQKEIRVKTDLIIRDSTK FT RALEF" FT RBS 1378..1383 FT /gene="licA" FT CDS 1391..5356 FT /transl_table=11 FT /gene="licA" FT /product="endo-1,3(4)-beta-glucanase" FT /function="endohydrolysis of 1,3-linkages in FT beta-D-glucans" FT /EC_number="3.2.1.6" FT /db_xref="GOA:Q59328" FT /db_xref="HSSP:1GU3" FT /db_xref="InterPro:IPR000757" FT /db_xref="InterPro:IPR001119" FT /db_xref="InterPro:IPR003305" FT /db_xref="InterPro:IPR008979" FT /db_xref="InterPro:IPR008985" FT /db_xref="InterPro:IPR013320" FT /db_xref="UniProtKB/TrEMBL:Q59328" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA61884.2" FT /translation="MYKRLLSSVLIIMLLLSAWSPISVQASDGINDIRGHWAEEDLNKW FT MEKGILVGYQDGTIRPDNNITRAEFVTLINKVFGLYELSREQFADVEDSKWYSREILKA FT RAAGYIAGYGSNVFKPDNYITRQEAVVIIAKVFELQSGSNYTSKFKDGSLVKEYAKDSV FT SALVEKGYIAGYEDGTFRPDNYITRAETIKILNKIIPSLYNEKGDYKNEEVAGNALINT FT EGVILKDTVINGDLYLAQGIQNGDVTLDGVNVKGTVFVNGGGSDSIHFINTKINRVVVN FT KTGVRIVTSGNTSVESVVVKSGAKLEEKELTGDGFKNVTVDSQLSAGNEIIFVGDFEQV FT DVLADDALLETKEAKMKLRIFGQRIKVNGKAIEKSSKNYIVNGELISTEEEPGPSDAPG FT AEDDQNSGSPGSSTNPAPTKNPNEEWRLVWSDEFNGSEINMANWSYDDPTNGRWNGEVQ FT SYTQNNAYIKDGALVIEARKEDITEPSGETYHYTSSKLITKGKKSWKYGKFEIRAKMPQ FT GQGIWPAIWMMPEDEPFYGTWPKCGEIDIMELLGHEPDKIYGTIHFGEPHKESQGTYTL FT PEGQTFADDFHVYSIEWEPGEIRWYIDGKLYHVANDWYSRDPYLADDYTYPAPFDQNFF FT LILNISVGGGWPGYPDETTVFPQQMVVDYVRVYQKDKYPHREKPAKEEVKPREPLEDGN FT YIYNGGFDVDDSAAVGVDGVPYTSYWTFLTASGGAATVNVEEGVMHVQIENGGTTDYGV FT QLLQAPIHLEKGAKYKASFDMKAENPRQVKLKIGGDGDRGWKDYAAIPPFTVSTEMTNY FT EFEFTMKDDTDVKARFEFNMGLDDNDVWIDNVKLIKTEDAPVIDPSEIARPPLLSGNYI FT YNGTFDQGPNRMGFWNFVVDSTAKATYYIGSDVNERRFETRIEKGGTSRGAIRLVQPGI FT NIENGKTYKVSFEASAANTRTIEVEIASNLHNSSIFATTFEISKESKIYEFEFTMDKDS FT DKNGELRFNLGGSNVNVYIDNVVMKRVSTDEVEGNLILNGVFNGLAGWGYGGYEPGSAD FT FESHEEQFRAIISSVGNEGWNVQLYQDNVPLEQGQTYEVSFDAKSTIDRKIIVQLQRNG FT TSDNNWDSYFYQEVELTNELKTFKYEFTMSKPTDSASRFNFALGNTENKTYAPHEIIID FT NVVVRKVATPSALILNGTFDDGMDHWLLYWGDGEGNCDVTDGELEINITKVGTADYMPQ FT IKQENIALQEGVTYTLSLKARALEARSIKVDILDSSYNWYGGTIFDLTTEDAVYTFTFT FT QSKSINNGVLTINLGTIEGKTSAATTVYLDDILLEQQ" XX SQ Sequence 5595 BP; 1872 A; 918 C; 1331 G; 1474 T; 0 other; tctagaggag tatgatatcg gcggcgcggt gtggaactac aaaaaaatgg attttgaaat 60 ttataatgag gatagaaaac ctgtctcgca agaattggta aatatactgg cgagaagaaa 120 aacttgatta ttaaaactac atttttgcaa aagtttgtaa tttaaaaaat acaacagctg 180 tttgaaagca tgtttttgct tcaaagtggt actgtaaata caaactttga ggggaggtgg 240 acgatgacca gtgaagaaat agcaaaatta tgtggtgttt ccagagccac ggtatccagg 300 gttattaaca acagtcccaa cgtaaaagag gaaacgcggc aaaagattct ggcagtgata 360 aaagaaaaaa attatgttcc gatagcgccg gcacggcgtt tggccgggat agacagcaat 420 ataattggcc tgtttgtttt ggatattgac atatctgagt caaagtcaag ggtctcggaa 480 agtacatact tttcacggct gataaatctg ataatagacc aggcaaacaa ttttggcttt 540 caagtattgg tgtcaattat aacttcacag aaacagctga gtgaaattag aaatctcttc 600 atgagcagaa ccattttcag cggcattttt atcggtgcgt tcaatgatga aatccaactt 660 gatgatgata ttataatgca acatcccaca attattattg accgccaatc agaaaggatg 720 gtgaaaaagc caaacagatt ggttgtaaac ctggacaact ttgagggtgc ttataatgcg 780 acacagtttt tgattaaatt ggggcatacc agaattgggc acatatccgg ggaccttaga 840 aaactttcgg gcatagaacg ctatgaagga tacaaaaaag cattggaaga tgcaggatta 900 ggttttgaca aaaatttggt tcgtgaaggg aacttccttg atgacagcgg ctataggctt 960 gcacgtgaga tattaaaaga gaacgtgacg gctattttct gtgccaatga tgtaatggca 1020 attagtgcaa ttaaagccat aaaagaaacg ggtttgagtg taccggatga tatatctgta 1080 ataggctttg ataatacagc aatcggaaat tatatcatgc ctgcattgac aactgtgaac 1140 gcgccgttgg agcatattgc agaagcatgt attgagtcat tgaaatactt ttgcgagcac 1200 aaacatttta aacaaaagga aatcagggtt aaaaccgatt tgataatccg ggattcaacc 1260 aagagggctt tggaattctg attgattgtt ttataatcat ccaaagatgt actcggatac 1320 atataattgg ggtaataatt acccctgagg tatttattat aaataaaaaa aatatgcagg 1380 agggtgaaaa atgtataaaa gattattgtc gtcagtactg ataattatgc tgttattatc 1440 agcctggtcg ccaatatccg tacaagcttc tgatggaatc aatgacatta gaggtcattg 1500 ggctgaagaa gacttgaaca aatggatgga aaaaggtatt ttggtgggct accaggatgg 1560 gacgataagg cccgataata atatcacaag agccgaattt gtcacattaa ttaacaaggt 1620 tttcgggctt tatgaattaa gccgggagca attcgcagat gttgaagact caaaatggta 1680 ttcccgtgaa atattaaaag ccagggctgc gggatatatt gcaggttatg gaagcaatgt 1740 tttcaaacct gacaattata ttacaagaca agaagccgtt gttataatcg cgaaagtttt 1800 tgaacttcaa agcggcagca attatacaag caagtttaaa gatggaagtc tggtaaagga 1860 atacgcaaaa gattccgtta gcgcgttggt tgaaaaaggc tacatagcag gttatgaaga 1920 tggcactttc aggccggaca actacattac ccgtgcagaa acaataaaaa ttctgaataa 1980 aattattcct tccttgtata acgagaaagg agattataaa aatgaagaag tagccggaaa 2040 cgctctgatt aacaccgaag gagttatttt aaaagatacc gtaataaacg gggatttgta 2100 tcttgctcag ggaattcaga acggcgatgt tacccttgac ggtgtgaatg taaaaggaac 2160 ggttttcgta aatggtggag gaagcgacag catacatttt ataaatacga aaataaacag 2220 ggttgttgtc aataaaacag gagttagaat tgtaacttcc ggcaatacct cggttgaaag 2280 tgttgtcgtt aaatccggtg caaaacttga agaaaaagaa ttgacgggcg acggctttaa 2340 aaacgttaca gtcgattctc aactttcagc cggcaatgaa ataatatttg tcggggattt 2400 tgaacaggtc gatgttctgg cggatgatgc cttgctggaa accaaagagg caaaaatgaa 2460 actgagaata ttcggccaaa ggattaaagt aaatggaaag gcaatagaaa aatcatcaaa 2520 gaactatatt gtaaacgggg aacttatatc aactgaggaa gaacccggtc cttccgacgc 2580 acccggtgcg gaagacgatc aaaattcagg tagtccgggc tcatcgacta atcctgcacc 2640 aaccaagaat ccgaatgaag agtggcgtct ggtttggagc gatgagttta acggttctga 2700 aataaatatg gctaattgga gctatgacga cccgaccaac ggaagatgga acggggaagt 2760 acaatcctac acacaaaaca atgcctatat caaagacggc gcgttggtta ttgaagcaag 2820 aaaagaagac attacggaac caagcggtga gacttatcat tatacatcgt caaagctgat 2880 taccaaaggc aaaaagtcat ggaagtacgg aaaatttgaa ataagggcaa aaatgccaca 2940 gggacaaggt atatggcctg caatctggat gatgccggaa gacgaaccct tctacggaac 3000 atggccaaag tgcggcgaaa tagatattat ggagcttttg ggccacgagc ctgataaaat 3060 ttatggaacg atccattttg gagagcctca taaagaatcc cagggaacgt ataccttgcc 3120 ggaaggccag acttttgctg atgatttcca cgtttattcg attgaatggg aaccgggaga 3180 aatacgctgg tatatagacg gcaagctgta tcatgtcgct aatgactggt actcgaggga 3240 cccgtacctt gccgatgact acacttatcc cgcacctttt gaccagaatt tcttcttgat 3300 tctcaatata tccgttggtg gcggctggcc gggatatcct gacgaaacga cagttttccc 3360 gcagcaaatg gttgtggact atgtgagggt atatcaaaaa gataaatatc ctcacaggga 3420 aaaaccggca aaggaagaag tgaagccaag agagcctctt gaggacggca attatatcta 3480 taacggcggt tttgatgtgg atgattctgc agcagttggt gtggacggtg ttccctatac 3540 gtcttactgg acattcttaa cagcatccgg tggagctgcg acagtcaatg tagaggaagg 3600 tgttatgcac gtacagatag aaaacggagg gacaaccgac tacggcgtac aattgcttca 3660 agctccgatt catcttgaaa aaggcgcaaa atataaagca tcttttgaca tgaaagctga 3720 aaatccaagg caggtaaaac tgaaaatagg cggagacggc gacaggggat ggaaagatta 3780 tgcggctatt ccaccgttta cggtctcaac agagatgacc aactatgagt ttgagtttac 3840 tatgaaagat gataccgatg ttaaggcacg gtttgagttt aatatgggtt tggacgataa 3900 tgatgtctgg attgacaatg ttaaactgat taaaacagaa gatgcgccgg ttatagatcc 3960 ttccgaaata gcaagacctc cgcttctttc cggcaactat atatacaacg gtacctttga 4020 ccaaggtccg aacagaatgg gattctggaa ttttgttgtg gatagcactg caaaggctac 4080 atactatatt ggaagcgatg ttaatgagcg caggtttgaa acaagaatag aaaaaggcgg 4140 aacatcgagg ggagccataa gattggttca gccgggaatt aacattgaaa acggcaaaac 4200 atacaaggtt agcttcgaag ccagtgcggc aaatacaaga actattgagg tggaaattgc 4260 aagcaatctt cacaacagca gcatttttgc gacaactttt gaaataagca aagagagcaa 4320 gatatacgaa tttgagttta caatggacaa agattcggac aagaacggag aacttaggtt 4380 caatctgggc ggaagcaacg tgaacgtcta tattgataat gtcgttatga aaagagtaag 4440 taccgatgaa gttgaaggaa acctgatttt aaacggggta tttaacggcc tggcaggctg 4500 gggatatgga gggtatgaac ctggatcggc agattttgaa agtcatgagg aacaatttag 4560 ggcaattatt agctctgtcg gtaatgaagg ttggaatgta cagttgtatc aggataatgt 4620 tccgctggaa caagggcaaa cctacgaagt ttcttttgat gcaaaatcaa cgattgacag 4680 aaagataatt gttcagctgc aaaggaacgg tacttcggat aataattggg actcctattt 4740 ctatcaagaa gttgaactta ctaatgaact taaaacattc aaatatgaat ttacaatgag 4800 taaacctaca gattcggcgt caagatttaa ttttgctttg ggtaatactg aaaacaaaac 4860 ttatgctcct catgaaataa taattgacaa tgttgtagta agaaaagttg cgactccttc 4920 tgcgctgata ttgaacggaa cctttgacga tggaatggat cattggctgc tatactgggg 4980 agacggtgaa ggcaattgcg atgtaactga cggagagctt gaaattaaca ttaccaaggt 5040 aggtaccgcg gattacatgc cgcagattaa acaggaaaac atagcgttgc aagagggtgt 5100 gacgtatact ttgtctctta aagcgagagc gcttgaggca agaagtatta aagtggacat 5160 attggattct tcttataact ggtatggcgg aactattttc gatttaacaa cggaagatgc 5220 cgtatacacg tttacattta cccaaagcaa gtcgataaat aacggtgtct taactataaa 5280 tttaggtacc atagaaggta agacatccgc cgcaactact gtctatcttg atgatatttt 5340 gctggaacaa cagtaagttg taagtttgta acggatgtaa aaataattaa cagttgagtt 5400 tttaaaaggc attatattgg ttgtaaaact gtataatgcc ttttttttat tgggtgcaac 5460 atttaacata cattttacaa agcgtttgat taaaatatga tataatttct ttgattttga 5520 tgaaaaggga atgggtgagg tatggatctg tttggttttt tagagcttat aggcggcttg 5580 tccctgttcc tcttt 5595 // |
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