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EBI DbfetchID X83963; SV 1; linear; genomic DNA; STD; PRO; 4516 BP. XX AC X83963; XX DT 09-OCT-1995 (Rel. 45, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 4) XX DE T.litoralis FOR, pki and pflX genes XX KW FOR gene; formaldehyde:ferredoxin oxidoreductase; pflX gene; pki gene; KW pyruvate formate-lyase; pyruvate kinase. XX OS Thermococcus litoralis OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae; OC Thermococcus. XX RN [1] RX PUBMED; 7642512. RA Kletzin A., Mukund S., Chan M.K., Rees D.C., Crouse T.K., Adams M.W.W.; RT "Molecular characterization of the genes encoding the tungsten-containing RT aldehyde ferredoxin oxidoreductase from Pyrococcus furiosus and RT formaldehyde ferredoxin oxidoreductase from Thermococcus litoralis"; RL J. Bacteriol. 177(16):4817-4819(1995). XX RN [2] RP 1-4516 RA Kletzin A.; RT ; RL Submitted (17-JAN-1995) to the EMBL/GenBank/DDBJ databases. RL A. Kletzin, Dept. of Biochemistry and Center for Metalloenzyme Studies, The RL University of Georgia, Life Science Building, Athens, GA 30602, USA XX CC Sequence overlapping with that submitted by S.Mukund and CC M.W.W.Adams (J.Biol.Chem. vol.268: 13592-13600; 1993) XX FH Key Location/Qualifiers FH FT source 1..4516 FT /organism="Thermococcus litoralis" FT /strain="DSM 5473" FT /mol_type="genomic DNA" FT /clone="plasmid pFOR 8a1" FT /db_xref="taxon:2265" FT promoter complement(680..715) FT /gene="pki" FT /note="putative (BoxA/BoxB)" FT CDS complement(<1..659) FT /transl_table=11 FT /gene="pki" FT /product="pyruvate kinase" FT /db_xref="GOA:Q56301" FT /db_xref="HSSP:1LIU" FT /db_xref="InterPro:IPR015793" FT /db_xref="UniProtKB/Swiss-Prot:Q56301" FT /protein_id="CAA58793.1" FT /translation="MVREMELPSHKTKIIATIGPASKQKESIKKMIKAGMSVARINFSH FT GTLEEHAKTIETVRDVAEKLERRVAILGDLPGLKMRVGKIKGDSVTLRKGDKVVLTTRD FT IEGDETTIPVEFKDLPKLVSKGDTIYLSDGYIMLRVEEVRENEVECVVVNGGILFSHKG FT INIPKANLPIEAITPRDFEIIEFAIEHGVDAIGLSFVGSVYDVLKVKSFLEKKSADL" FT CDS complement(1222..2274) FT /transl_table=11 FT /gene="pflX" FT /product="pyruvate formate lyase activating protein FT homologue" FT /db_xref="GOA:Q56302" FT /db_xref="InterPro:IPR016431" FT /db_xref="UniProtKB/TrEMBL:Q56302" FT /protein_id="CAA58794.1" FT /translation="MIKTQLMTMWFEIKRAKDALPHYFSILSGEEKPNFFYSKQVEVNF FT GEDEDLESLWETHEEGMEKLRENDLKENPKKSLLDLKALIAYKMLESCELCEFKCHANR FT FEEIGYCRVKESLVASDFLHIGEEPELVPSYTVFFSGCNFRCAFCQNWDISQYRVGLRY FT SPKEMAVKIAVAYAEGAKNVNFVGGEPTPNLPFILETLKYVKVPIPVVWNSNMYMSEKS FT MKLLDGIVDVYLGDFKWGNDEDALKYSKAPRYWETVTRNFLLAKNHYKAEFLIRHLVMP FT GHLECCTRPILEWISENLGRDVRVNVMFQYRPEYRAHEYPEIDRRLTNDEMLKAAELVK FT EFRLKNALVG" FT terminator complement(1162..1187) FT /gene="pflX" FT /note="putative" FT promoter 2460..2491 FT /gene="FOR" FT /note="putative" FT CDS 2499..4364 FT /transl_table=11 FT /gene="FOR" FT /product="formaldehyde:ferredoxin oxidoreductase" FT /db_xref="GOA:Q56303" FT /db_xref="HSSP:1B25" FT /db_xref="InterPro:IPR001203" FT /db_xref="UniProtKB/Swiss-Prot:Q56303" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA58795.1" FT /translation="MKGWWGKILRVDLTNNKVWVQEYSPEVAKNFIGGRGLAAWILWNE FT AKNVDPLGPKNKLVFATGPFNGLPTPSGGKMVIAAKSPLTGGYGDGNLGTMATVHLRKA FT GYDALVVEGKAKKPVYIYIEDDNVSILSAEGLWGKTTFETERELKEIHGKNVGILSIGP FT GGENLVKYAVVISQEGRAAGRPGMGAVMGSKKLKAVVIKGTKEIPVADKEKLKELSQEA FT YDAILNSPGYPFWHRQGTMAAVEWTNENSALPTRNFSDGSFEFARSIDGYTMEGMKVKQ FT RGCPYCNMPCGNVVLDAEGQESELDYENVALLGSNLGIGKLNEVSVLNRIADEMGLDTI FT SLGVAISYVMEAKEKGIIKDDDAPEFGDFKKAKQLALDIAYRRGELGNLAAEGVKVMSE FT KLGAKDFAMHVKGLEVSGYNCYIYPAMALAYGTSSIGAHHKEAWVIAWEIGTAPIEGEK FT AQKVEYKITYDPEKAAKVIELQRLRGGLFEMLTACRLPWVEVGLSLDYYPKLLEAITGV FT KYTWDDLYKAADRVYALMRAYWVREFNGNWSREMDYPPERWFKEGLKSGPYKGQHLEKD FT KYDALLSEYYKLRGWDERGIPKKETLKELNLEFVIPELEKVTKLE" FT terminator 4367..4392 FT /gene="FOR" FT /note="putative" XX SQ Sequence 4516 BP; 1249 A; 1002 C; 983 G; 1282 T; 0 other; agatctgcac ttttcttttc aagaaagctc tttactttga gcacatcata caccgaacct 60 acaaaagata gacctatggc atctacccca tgttcaattg cgaactctat tatctcaaag 120 tcccttggag ttatcgcctc tatcgggaga ttcgcttttg gaatgtttat ccccttatgg 180 gagaacagta tccctccgtt aactacaaca cattcgacct cattttccct gacttcctca 240 accctcagca ttatgtagcc atcgctcagg tatatggtat caccttttga aaccagcttt 300 ggtaagtctt taaactcaac tggaatagtt gtttcgtcgc cttcaatatc tctcgtcgtg 360 agcacaactt tatctccctt ccttagagtc actgagtccc cctttatctt tcccactcgc 420 atttttaatc ccggcaaatc gcctaaaatt gcgactctcc tttccaattt ctcggcgaca 480 tctctcactg tctcaatggt ctttgcatgc tcttcaagag ttccatggga gaagtttatc 540 ctcgcaacac tcattccggc ttttatcatc ttttttatgg actctttttg cttagaagcc 600 ggccctatcg tggcaattat ctttgtctta tgagagggaa gctccatttc tctcaccatt 660 ttctttttcc atctggaatt aattaagttt tttggaattc gaaagatatt ttaaagccat 720 cctcttaact ttcttcatga gaagaaaact gataaccgca attccccccg aggagattct 780 taaaatcaag gagggttcca aggcgagggt tgatataaaa atccgagaag ctgatgccta 840 ttttggaatg cccagatggg agataatcat tgatgggagc aacgaggaaa tcgagaagtt 900 catgaacgtc ttgatgaagt ctagggcggg aggctagtaa agctctccaa ttttttcgtt 960 ttcctcaagt tctttctcct ttttctccag gtatttggag taccaagcgg tatagatgcg 1020 aaagattaga taaaccgcta ccaagcttag tattgctcct acaagctgtc tatctgtaac 1080 ttctctcagc ggaatgggca tctatcttcc cccttttcgt tccaaatatt cttctaagag 1140 tgttttcaag ccgaagagaa tgaaaaacag cagaaaaaga attaaaaccc attttgcgga 1200 aactccagat gtaacctgca tttatcccac cagcgcgttc tttaatctga attcttttac 1260 aagttcagcc gctttaagca tctcatcgtt tgtgagcctt ctatcaattt cgggatattc 1320 atgggccctg tactctggac ggtattggaa cattacattg accctaacgt ccctgccgag 1380 attttcgctt atccactcta aaattggcct tgtgcagcac tctaaatgac ccggcattac 1440 aagatgcctt attaaaaact cggctttgta gtggttttta gctagtaaaa agttccttgt 1500 aactgtttcc cagtatctgg gagcttttga gtatttaagt gcatcttcat cgttacccca 1560 tttgaagtcc ccaaggtaga catcgacaat cccatccaaa agtttcatgc ttttttcgct 1620 catgtacatg ttggagttcc acactaccgg aatcggaact ttgacatact ttagagtctc 1680 aaggataaaa ggaagatttg gagttggctc gccaccaacg aagttaacgt tctttgcccc 1740 ctcagcatat gccaccgcta tcttaacggc catctccttg ggggaatacc tcaagcctac 1800 gcggtattgg cttatatccc agttctgaca gaaggcgcac ctaaagttgc acccggaaaa 1860 aaagactgta taagacggca caagctccgg ctcctcccct atgtggagaa aatcactcgc 1920 cactaaactt tctttcactc tgcagtagcc gatctcttca aatctatttg cgtggcattt 1980 aaactcgcac agctcacagc tttcaagcat tttatatgct attagggctt ttaaatcgag 2040 gagactcttt ttgggattct cttttaaatc gttctctctc aacttttcca tgccttcttc 2100 gtgtgtttcc cacagactct caaggtcctc atcttctccg aaattaacct ctacctgctt 2160 tgaatagaag aaatttggct tttcttctcc gcttaagatt gaaaagtaat gcggtaatgc 2220 atcttttgcc cttttaattt caaaccacat ggtcatcagt tgtgttttga tcattgagat 2280 tatttaaatt cttttgtggt tttatggagt aatcgaattc atacgaacca aatcagccat 2340 ctgttgcaaa tttttcgaaa ccttttgata aataagaaaa cttatttgaa atgttttttg 2400 taaacaactt tgttttcaca atttgctaaa agtaaactat tttatgaacc caaaaccttt 2460 attaaaattc aagcattaat gaactctggt ggtcttctat gaaaggctgg tggggaaaaa 2520 tcctaagggt tgatttaact aacaacaaag tctgggtgca ggaatattct ccggaagttg 2580 caaagaattt tattggtggt agaggtctag cagcctggat tctttggaac gaagcaaaga 2640 acgtagatcc tcttggccca aagaacaagc ttgtatttgc caccggtcct ttcaacggtc 2700 ttccaacccc aagcggcgga aagatggtta tagctgctaa gagcccccta acaggcggtt 2760 atggtgatgg gaaccttgga acaatggcta ctgtgcattt aagaaaagcc ggttatgatg 2820 cgctcgttgt tgaaggtaag gccaaaaagc cggtttacat ctacatagag gatgataacg 2880 ttagcatttt gagtgctgag ggcctctggg gcaaaaccac ctttgaaact gaaagggaac 2940 tcaaagagat acacggcaaa aacgtcggaa ttctaagcat aggtcccggt ggagagaacc 3000 tcgttaagta tgccgttgtt atctcccagg aaggaagggc tgccggaaga cccggtatgg 3060 gtgcagtaat gggaagcaaa aagctcaagg cggtagttat caagggtacc aaggaaattc 3120 ccgttgcgga caaggagaag cttaaagaac tttcacaaga agcctacgat gcaattctca 3180 actcacctgg ctatccgttc tggcacagac agggaacgat ggccgctgtg gaatggacaa 3240 atgagaactc cgccttgccg acgaggaact tcagcgacgg ctcttttgag ttcgcccgct 3300 caatagacgg ctacacaatg gaaggtatga aggttaagca gaggggctgt ccatactgta 3360 acatgccctg tggaaacgtg gttcttgatg ctgaaggtca ggagagtgag ctggactatg 3420 aaaacgtcgc cctattgggt tcaaaccttg gcataggaaa gcttaacgag gtctcagttc 3480 tcaacagaat tgccgacgag atgggccttg atacaatctc gctgggagtc gcgatatcct 3540 acgtgatgga agctaaggag aagggcataa taaaagatga cgatgctcca gaatttggtg 3600 acttcaagaa ggccaagcag cttgcattgg acatagccta cagaaggggt gaacttggaa 3660 accttgcagc cgagggtgtc aaagtgatga gcgaaaagct cggtgccaag gactttgcaa 3720 tgcacgtaaa aggtcttgag gtcagcggtt acaactgtta catctatccg gcaatggcct 3780 tggcatatgg aactagttca atcggtgctc accacaagga agcgtgggtc attgcatggg 3840 aaattggtac cgcaccaatt gaaggcgaaa aggcccagaa agttgagtac aagataacct 3900 acgacccaga aaaggccgca aaggttatcg agctccagag acttagaggt ggcctctttg 3960 agatgctcac tgcctgtaga ttgccatggg ttgaagttgg cctaagcttg gactactatc 4020 caaagcttct tgaagcaata accggcgtca agtacacctg ggacgacctc tacaaggcag 4080 ccgatagggt ttatgctctc atgagggcat actgggttag ggaattcaac ggcaactgga 4140 gcagagaaat ggactatcca ccagagagat ggttcaaaga aggccttaag agcggcccat 4200 acaaaggcca gcacttggag aaggacaagt atgacgctct actcagcgaa tactacaagc 4260 tcaggggctg ggacgagagg ggtattccaa agaaggagac acttaaggag ctcaaccttg 4320 agttcgtcat cccagagctt gagaaggtta cgaagctcga gtgagctttt ttctttatct 4380 tttttgtctc ttgggcttct tgacttctct tattttgaaa agagattttc aaaagaaaat 4440 gaaaaaagtt caagtaagac aatcatactc atgatgactt gttccctaat atcagttctt 4500 tgtggatttt ggatcc 4516 // |
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