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EBI DbfetchID X82105; SV 1; linear; genomic DNA; STD; PRO; 5360 BP. XX AC X82105; XX DT 17-OCT-1994 (Rel. 41, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 2) XX DE E.coli 47IIR, 47IIM, 47IR genes XX KW DNA methyltransferase; eco47IIM gene; eco47IIR gene; eco47IR gene; KW restriction endonuclease. XX OS Escherichia coli OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. XX RN [1] RP 1-5360 RA Stankevicius K.; RT ; RL Submitted (07-OCT-1994) to the EMBL/GenBank/DDBJ databases. RL K. Stankevicius, Inst. of Biotechnology Fermentas, Graiciuno 8, Vilnius RL 2028, LITHUANIA XX FH Key Location/Qualifiers FH FT source 1..5360 FT /organism="Escherichia coli" FT /strain="RFL47" FT /mol_type="genomic DNA" FT /db_xref="taxon:562" FT CDS 365..1084 FT /transl_table=11 FT /gene="eco47IIR" FT /product="type ii site-specific deoxyribonuclease" FT /EC_number="3.1.21.4" FT /note="ORF1" FT /db_xref="GOA:P50195" FT /db_xref="InterPro:IPR019057" FT /db_xref="UniProtKB/Swiss-Prot:P50195" FT /protein_id="CAA57628.1" FT /translation="MSLLEYISDEDLFNEVETLLTKAKKKKDAAEKTFTSNVIDPFGAL FT FEAPGFSSHEEWRNSELARQQQKTIQNHVGTFHQKILGHVEGWRDMGIGGIVDLLNEER FT RIIAEVKNKYSTVTGGDLADKYKGLDELVSPKHSRFKDYCAYFVNIIPRKPTRYNSPFT FT PSNKGSGTLCPSNPNIRIIDGASFYELVTGRPDALQELHSALPHAIEYILSERLGQQGF FT SIPDKDSFIKYFGLAYG" FT CDS 1144..2397 FT /transl_table=11 FT /gene="eco47IIM" FT /product="site-specific DNA-methyltransferase FT (cytosine-specific)" FT /EC_number="2.1.1.73" FT /note="ORF2" FT /db_xref="GOA:P50196" FT /db_xref="HSSP:6MHT" FT /db_xref="InterPro:IPR018117" FT /db_xref="UniProtKB/Swiss-Prot:P50196" FT /protein_id="CAA57629.1" FT /translation="MLAWLLNMLKEEFSLSEVADILGVSKETLRRWDTAGKLVSQRNDE FT NNYRFYKKEQLKNFEQAQFLFKSQWPDETKISNNVYTVLELFAGAGGMALGLEKAGLKS FT VLLNEIDSHACKTLRKNRPEWNVVEGDVSQVDFTPYRNTVDVLAGGFPCQAFSYAGKKL FT GFEDTRGTLFFEFARAAKEINPKVLLAENVRGLLNHDAGRTLETIKNIITDLGYTLFEP FT RVLKAIFYKVPQKRERLIIVAVRNDLADGIDYEWPSSYNKILTLKDALKKGELYDSDVP FT ESEGQKYPKRKAEILSMVPPGGYWRDLPEDIQKEYMLKSFYLGGGKTGMARRLSWDEPS FT LTLTCAPAQKQTERCHPEETRPLTVREYARIQTFPDEWVFEGPMSAKYKQIGNAVPVNL FT SFAVGKSVVHLLDKINKR" FT CDS complement(3816..4508) FT /transl_table=11 FT /gene="eco47IR" FT /product="Eco47I restriction endonuclease" FT /db_xref="GOA:P50194" FT /db_xref="InterPro:IPR019070" FT /db_xref="UniProtKB/Swiss-Prot:P50194" FT /protein_id="CAA57630.1" FT /translation="MSKETSFVKNAEELAKQKMDAINPELSSKFKFLIKFLSQFPEACS FT KPRSKKMQNKVGQEEHIEYLARSFHESRLPRKPTPPTTVPDEVVSIVLNISFNIQPENL FT ERIKEEHRLSMAAENIVGDLLERYLAEKLEPSGWIWCSGTSVKAVDFIHYDEKNNEWNL FT LQVKNRDNTENSSSSKIRDNTTIKKWFRTYSQRDATNWDNFPDEVSSKNLNEEDFRAFV FT KNYLVKII" XX SQ Sequence 5360 BP; 1539 A; 1119 C; 1131 G; 1570 T; 1 other; gggggggaga gcagacgaag gtctatgtct ttcttgatga taagatgaag gtggtaacga 60 ctattggtgt atcgcgatat atagaattaa ttatttatct taaaatttag gtgttaaaat 120 cctctatcac tgtaacatat tgctcgaatc ctcagcacaa gccattcaaa tcccgccaga 180 acaaacatac gccctttaag tgnttattga tcgttttcat ctatgtctgt aatcgttatg 240 attagaaaac ttgctccagt gtcgtttttg agagctgtcg tgtaattagc cgcaaccgca 300 atagtcccca aaggttatta cgcgaaaaac ttattgaatt ataaagaatg atggagagac 360 aactatgtca ttacttgaat atatcagtga tgaagatctt tttaacgaag ttgaaacctt 420 attaacgaaa gctaaaaaga aaaaagacgc cgctgagaag acttttacca gcaatgtgat 480 tgaccctttt ggtgcacttt ttgaagctcc ggggttctca tcccatgaag agtggcgaaa 540 ctccgaactt gcacgacaac agcagaaaac cattcaaaat catgttggta cgtttcacca 600 aaaaattctc ggccatgttg aaggatggag ggatatgggg attggtggca tcgttgatct 660 tctcaacgaa gaaagaagaa tcatcgctga ggtgaaaaat aaatactcaa cggttactgg 720 cggggattta gcagataaat ataaaggctt agatgagttg gtatcaccga aacatagccg 780 atttaaggat tactgtgcgt actttgttaa tataatccct cgtaaaccta ctagatataa 840 cagccccttt actccttcca ataaaggtag tggtactctg tgtccttcga accctaacat 900 tagaatcatt gatggtgcga gtttctatga gcttgtcact ggcagaccag atgctctgca 960 agaactccat agtgctctcc ctcacgcaat tgagtatatt ttgagcgaac gtcttgggca 1020 gcaaggtttt tccatccctg ataaagatag ttttattaag tattttgggc tcgcttacgg 1080 ctgataacca tgatcaatgt ttgacaaagc acatgtaaac ccatacagta gtaaccatga 1140 ctaatgttgg catggttact aaatatgtta aaggaagagt tttcactttc agaagttgca 1200 gacattttgg gcgtttcaaa agaaacttta aggcgttggg atactgctgg aaaattagtt 1260 tctcaaagaa atgacgaaaa caactatcga ttttataaaa aagagcaact taaaaatttt 1320 gaacaagctc agtttttatt taaaagccag tggcctgatg agactaaaat aagcaataat 1380 gtttatactg tattagagtt atttgctggc gcagggggga tggctttagg tttagaaaaa 1440 gccggtttaa aatctgtttt actaaatgaa attgactccc atgcttgtaa gacgttacga 1500 aaaaataggc ctgaatggaa tgtggttgaa ggtgatgtga gccaagtaga cttcacccct 1560 tataggaata ccgttgatgt gctggctggt ggctttcctt gccaggcatt ctcttatgca 1620 ggcaaaaaac ttggttttga agatacacgg ggcacccttt tctttgaatt cgcccgagcc 1680 gctaaagaaa tcaatccgaa agttctttta gcagagaatg ttcgagggtt gctaaatcat 1740 gatgctggac gaactttaga aacaataaaa aatattatca cagacttggg ctacacttta 1800 tttgagccaa gagtgcttaa ggctattttc tacaaagtgc cgcaaaaacg cgagcgtttg 1860 atcattgtag ctgtaagaaa tgatcttgct gatggcatcg attatgagtg gccttcttct 1920 tacaataaaa tattaaccct taaagatgca ttaaaaaagg gagagctgta tgatagcgac 1980 gtgccagaat ctgaaggaca aaaatatccc aaaagaaaag cagagatcct aagtatggtt 2040 cctcccggtg gctactggag agatcttcct gaagatattc aaaaagaata catgctcaag 2100 agtttttact taggtggggg caaaactggt atggctcgtc gtttgtcatg ggatgaacca 2160 agcctaacat taacatgcgc cccagcacag aaacaaacag agcgttgcca cccagaagaa 2220 acaagaccat taactgtgcg tgagtatgca agaatacaga ccttccccga tgaatgggta 2280 tttgaaggcc caatgtcagc gaaatataag caaataggaa acgctgttcc tgttaatctg 2340 tcatttgctg ttggcaaatc tgtggtacat cttttagata agataaataa aagatagacc 2400 ctgtaaataa ttctgtgtaa ttgctgccat attaaaggtg atcgctcagg cggtcaccga 2460 actcgataat aaagcgactc atcgccagcc gccagctctg gattggcata ttccattttt 2520 ttgatgcatc cttgatcgcc agagaaatga ccttccgcag cgagtcgtca gtcgggaaca 2580 ctttacgctt cttaatggcc gcacggatca cgctgttcag cgattcgata gcgttcgtgg 2640 tgtagatggc cttgcggata tcgggcgaat agcgaagaac gtgttgatat tttcccagtg 2700 cgcacgccag cttttgctga tttgcgggta tttatcgtcc cagacattcg ggaactgctc 2760 cggtgccact agcgccgcct cttctgttgg cgcctgatac accgttttta acccgccagt 2820 gacggctttg tagtccttcc acgatacgta tttcaggctg ttgcgcacca tatgaatgat 2880 gcacaactgg atgtgcgtct acggatacac gctgtttatc gcatccggaa agcctttcag 2940 accgtccatg caggcaataa ggatatcctg aagcccccga ttcttaagct ctgtcagccc 3000 ccccagccag aacttcgccc cttcgttctc ggccagccac atgcccagca actctttctg 3060 gcctccagta ttaataccga gtgcaaggaa caccgctttg ttaattacgg tgccaccttg 3120 acgaactttc accacgatac agtcaaggta aacaatggga tacagtgcat ccagaggtcg 3180 attttgccat tctgcaacct gctctttgac cgcatcagtg actttacata tcagcgtggg 3240 tgacacatct gcgtcgtaca tctctttgaa ggtggcgaca atttcgcggg tagtcatatc 3300 tttggcgtag agggataaaa tctggctgtc catctgcgta atgcgcgtct ggtgcttctt 3360 aatcaactgc ggttcgaagg tgttttcacg gtcacgcgac gtgttcagtt cgatctcgcc 3420 gtcatcgcat aacaccgttt tgacgagtag ccattgcggg tgtttgagcc tgttttgggc 3480 acatttctct catacccgag gtggtcagtc agctccgcat tgagcgccgt ttcgacggta 3540 cgcttcgtca acatgcggga aaaagcattg agatctgctt cggttttaag gcctttagcc 3600 agttcagccg caagggcttt gagtttcttt tcgtccataa ttgcctgtct ccgttactgg 3660 agtgaacata tcaaaatcag gcaattacac aattttaatc acagtctcta cagggtaggt 3720 ttcatggcct acccttttat tcttactcta tgttggatgc gttagattta ttccagaata 3780 attattatga taaaaatgaa gtgttttcct cctctttata ttatttttac caaataattt 3840 ttaacaaaag ctctgaagtc ttcttcattt aaattcttag atgatacttc atctggaaaa 3900 ttgtcccaat tagtagcatc acgctgtgag taagttctaa accatttttt tattgttgta 3960 ttatctctaa tcttactact cgatgagttt tcggtattat ctctattttt aacttggagc 4020 aggttccatt cattgttttt ttcatcatag tgaataaaat caactgcttt aacacttgtg 4080 cctgaacacc atatccatcc agaaggttct agtttttcag caagatacct ttcaagcaaa 4140 tcacctacaa tattctcagc agccatcgac aatctatgtt cttcttttat tctctcaaga 4200 ttttcaggct gtatattaaa acttatatta agaactatgc taaccacctc atcaggaacc 4260 gttgtaggtg gcgtgggttt tcttggcaat cgactctcat gaaaactacg agctaaatat 4320 tcaatatgtt cctcttgacc aactttattc tgcatttttt ttgaacgagg tttagagcaa 4380 gcttcaggaa actgagacag gaattttatt aaaaatttaa attttgaaga aagttcaggg 4440 ttaatagcat ccattttttg ctttgcaagt tcctcagcat tcttaacaaa agacgtctct 4500 tttgacattg tattctcccg acatccatta agtgtttgga tgtagtataa ttttctgact 4560 ttgaaagtta cagcaaaagt tttactctct cacaacttat gtcgtggttt actgaatttg 4620 gccacctgaa cagaagtgat aagcccacct cataacaaca caggtgcacc aatgaaaaga 4680 agaagtttta gtccagaatc caaacgcgaa tccactcaac tggttgttga ccagaactac 4740 acagtggcag ataccttcaa agctatggat gtcggccttt ccacgattac aaggtgggtc 4800 aaacaactgc gtaatgagtg tcagggcaaa acacccaaag cctcccccga taactccgga 4860 acattatcag gataactgtg tacctttgca ggggcatggc tgaaatgctg gcagagcctg 4920 catatcgttt acagatacac accaccatag acttgtttac gccgccattc agatctcaaa 4980 atcagataca ggaagacagc acatcgggct tgaatgctta gttagtctgc tttggaagga 5040 tttatagacg tggtatcgtc atcagcttcg agtttttaat acctgttcga gcagtacaac 5100 tagaggccgt tacagttaag atctcccatt accatcatca accatagcgg cagtccaagg 5160 taatagatta cgggaaaaga ttagtagcaa tagacagggt tttcaccata actcgttgaa 5220 aaacaagtta ttgcatacgg tatggtgccg aagcgcggac tcgaaccggc acgtatttct 5280 acggttgatt ttgaatcaac tgcgtctacc gatttcgcca cttcggcact gaagaggtat 5340 gcggaaaacg ttgtggatta 5360 // |
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