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EBI DbfetchID X81638; SV 1; linear; genomic DNA; STD; PRO; 2126 BP. XX AC X81638; XX DT 21-SEP-1994 (Rel. 41, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Bacillus sp. plasmid pXH13 Bsp6I restriction endonuclease and DE methyltransferase genes XX KW bsp6IM gene; bsp6IR gene; methyltransferase; restriction endonuclease; KW site-specific desoxyribonuclease. XX OS Bacillus sp. OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. OG Plasmid pXH13 XX RN [1] RP 1-2126 RA Lubys A.; RT ; RL Submitted (14-SEP-1994) to the EMBL/GenBank/DDBJ databases. RL A. Lubys, Institute of Biotechnology Fermentas, Graiciuno 8, Vilnius 2028, RL LITHUANIA XX RN [2] RX DOI; 10.1016/0378-1119(94)00795-T RX PUBMED; 7607501. RA Lubys A., Janulaitis A.; RT "Cloning and analysis of the plasmid-borne genes encoding the Bsp6I RT restriction and modification enzymes"; RL Gene 157(1-2):25-29(1995). XX FH Key Location/Qualifiers FH FT source 1..2126 FT /organism="Bacillus sp." FT /plasmid="pXH13" FT /strain="RFL6" FT /mol_type="genomic DNA" FT /db_xref="taxon:1409" FT CDS 488..1012 FT /transl_table=11 FT /gene="bsp6IR" FT /product="type ii site-specific deoxyribonuclease" FT /EC_number="3.1.21.4" FT /db_xref="GOA:P43419" FT /db_xref="InterPro:IPR019037" FT /db_xref="UniProtKB/Swiss-Prot:P43419" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA57292.1" FT /translation="MALHDFVKIDKSRFEDAVKAYFLWKELNALIKNSHNRGINFPETI FT SETLLCAAMGFELNRGTGGDAREPKTQAIIESKATSNWDRDTTSFSPSENFDALYFLRL FT NQRDDELYIYNTGINSDDLKSIQVNKTQTLGDQQAQGRRPRFSVIKFIIEANDIEPVAK FT VELRTKKIIKL" FT RBS 467..470 FT /gene="bsp6IR" FT CDS 1112..2059 FT /transl_table=11 FT /gene="bsp6IM" FT /product="site-specific DNA-methyltransferase FT (cytosine-specific)" FT /EC_number="2.1.1.73" FT /db_xref="GOA:P43420" FT /db_xref="InterPro:IPR001525" FT /db_xref="InterPro:IPR018117" FT /db_xref="UniProtKB/Swiss-Prot:P43420" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA57293.1" FT /translation="MLQIASLFAGVGGIDLGFEQTGYFETVWANEYDKNAAITYQSNFK FT NKLIIDDIRNIKVEDVPDFDVLLSGFPCTSFSVAGYRKGFEDEKSGDLFFETLRLIVAK FT KPQVIFLENVKNLVGHDNGNTFKVIYEALESNGYHIKYQVLNAKDFGNIPQNRERIYIV FT GFRNIEHYKNFNFPMPQPLTLTIKDMINLSDKLDDRFYYTEDKCSFYSPLQEQMTSDET FT IYQWRRKYVRENKSNVCPTLTANMGTGGHNVPLVKTKHGIRKLTPRECFNFQGYPEDFI FT LPELAPTHLYKQAGNSVVVPVIRRIAENIYKSML" FT RBS 1102..1105 FT /gene="bsp6IM" XX SQ Sequence 2126 BP; 760 A; 317 C; 395 G; 654 T; 0 other; gatcttcggg ggatgcccct tcggcgggcg caaacaaacg gggtttgttt gaatcaatag 60 gctttgggtg cagatgaatt agtcaactcc ttaatgctca cttcgttccg cttaccgttg 120 actaataatt tattcttctt cttctcttct ttaattcttg ttatagttcc gctatcgttc 180 cgcattcgtt tctttaaagg ttctttatcc gttcctcagc aatgcaaaga ggtgttaaat 240 ttggtcgccc taccatcgat gtgccagaca attggaatga actctatgag caatggaaag 300 ctaaggagat aacagcagtg aagtttataa ccgaagttag tatgaccaaa gcaacgtttt 360 accgtaaagt taaagaatat gaaaaaagtg agcagatgga tacccccata taaatgaata 420 agatgattat atttaatgat taagttaata tattactaga tacactagga agcacacata 480 aacaaacatg gcactacatg attttgtaaa aatagataag tctagatttg aagatgcggt 540 aaaggcatat ttcctttgga aagagttaaa tgctcttatc aaaaacagtc acaatagagg 600 aatcaatttc cctgaaacta tttcagagac acttttatgt gcagcaatgg gatttgaatt 660 aaatagagga accggtggcg acgctaggga gcctaaaacc caagcaatca ttgaatcaaa 720 agcaacatca aattgggata gagatacaac ttctttttct ccaagtgaaa actttgatgc 780 attatatttt ttaagattga atcagcgaga tgatgaactt tatatttaca atactggaat 840 taattccgat gatttgaaaa gtattcaagt aaacaaaacg caaacacttg gagatcaaca 900 ggcacaaggt cgtcgtccaa gattcagcgt tattaaattc atcattgaag cgaatgacat 960 cgaacccgta gcaaaagttg aattacgaac gaagaaaatt atcaagttat aaaaagcaaa 1020 ggaggtagtg tgcttccttt gcttttaaat tgcggcgcgc cgcaatttat gctataattt 1080 gataatcatg taaaggaata gaggataaaa gatgttacaa atagcaagtt tattcgcagg 1140 tgtaggtgga attgatttag gatttgaaca aactggatat tttgaaacgg tttgggcaaa 1200 tgaatatgat aaaaatgcag ctattaccta tcaatcaaat tttaaaaaca aattaattat 1260 agatgatatt cgaaatatca aagtagaaga tgttcctgat tttgatgttt tattatcagg 1320 atttccttgt acttctttta gcgtggctgg atatagaaaa ggctttgaag atgaaaaaag 1380 cggagattta ttttttgaaa ctttacgctt gattgtagct aaaaaaccac aagttatatt 1440 tttagaaaat gttaaaaatc ttgtgggtca tgacaacgga aataccttta aagtcattta 1500 tgaagcgtta gaaagcaacg gatatcatat aaaataccaa gttctaaacg caaaagattt 1560 tgggaatata cctcaaaata gagagcgtat ctatattgta ggttttagga atattgaaca 1620 ctataaaaat tttaattttc cgatgccaca accacttaca ttaacaataa aagatatgat 1680 aaatctttca gataaactag atgatagatt ttattataca gaggataaat gttcttttta 1740 ttcaccttta caagaacaaa tgacttcaga tgaaacaata tatcaatggc gtagaaaata 1800 tgtgagagaa aacaaaagta atgtatgccc tacgttaact gcgaacatgg gaactggagg 1860 acataatgtt ccattagtta aaacaaaaca tggaattaga aagttgactc ctagagagtg 1920 tttcaatttt caaggttatc ctgaagattt cattttaccc gaattggctc ctacgcatct 1980 ttataagcaa gctggaaatt ctgttgttgt tcctgtcata agacgcatag cagaaaatat 2040 ttataaatct atgttgtaat taaatggttt tatatactgg cggtgaatat tttgaaaaat 2100 ttaatcaagc attagatgaa gcgatc 2126 // |
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