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EBI DbfetchID X80993; SV 1; linear; genomic DNA; STD; PRO; 4183 BP. XX AC X80993; XX DT 11-AUG-1994 (Rel. 40, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Clostridium thermocellum cbhA gene XX KW cbhA gene; cellulose 1,4-beta-cellobiosidase. XX OS Clostridium thermocellum OC Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; OC Clostridium. XX RN [1] RX PUBMED; 9620957. RA Zverlov V.V., Velikodvorskaya G.V., Schwarz W.H., Bronnenmeier K., RA Kellermann J., Staudenbauer W.L.; RT "Multidomain structure and cellulosomal localization of the Clostridium RT thermocellum cellobiohydrolase CbhA"; RL J. Bacteriol. 180(12):3091-3099(1998). XX RN [2] RC revised by [3] RA Zverlov V.V.; RT ; RL Submitted (10-AUG-1994) to the EMBL/GenBank/DDBJ databases. RL V.V. Zverlov, Inst for Microbiology, Technical University Munich, Arcisstr RL 21, 80290 Munich, FRG XX RN [3] RP 1-4183 RA Zverlov V.V.; RT ; RL Submitted (22-JUN-1998) to the EMBL/GenBank/DDBJ databases. RL V.V. Zverlov, Inst for Microbiology, Technical University Munich, Arcisstr RL 21, 80290 Munich, FRG XX FH Key Location/Qualifiers FH FT source 1..4183 FT /organism="Clostridium thermocellum" FT /strain="F7" FT /mol_type="genomic DNA" FT /clone_lib="pUC19" FT /clone="PUC303" FT /db_xref="taxon:1515" FT CDS 439..4131 FT /transl_table=11 FT /gene="cbhA" FT /product="cellulose 1,4-beta-cellobiosidase" FT /EC_number="3.2.1.91" FT /db_xref="GOA:Q59325" FT /db_xref="InterPro:IPR016134" FT /db_xref="PDB:1RQ5" FT /db_xref="UniProtKB/TrEMBL:Q59325" FT /protein_id="CAA56918.1" FT /translation="MKFRRSICTAVLLAVLLTLLVPTSVFALEDNSSTLPPYKNDLLYE FT RTFDEGLCYPWHTCEDSGGKCSFDVVDVPGQPGNKAFAVTVLDKGQNRWRVQMRHRGLT FT LEQGHTYRVRLKIWADASCKVYIKIGQMAEPYAEYWNNKWSPYTLTAGKVLEIDETFVM FT DKPTDDTCEFTFHLGGELAATPPYTVYLDDVSLYDPEYTKPVEYILPQPDVRVNQVGYL FT PEGKKVATVVCNSTQPVKWQLKNAAGVVVLEGYTEPKGLDKDSQDYVHWLDFSDFATEG FT IGYYFELPTVNSPTNYSHPFDIRKDIYTQMKYDALAFFYHKRSGIPIEMPYAGGEQWTR FT PAGHIGIEPNKGDTNVPTWPQDDEYAGIPQKNYTKDVTGGWYDAGDHGKYVVNGGIAVW FT TLMNMYERAKIRGLDNWGPYRDGGMNIPEQNNGYPDILDEARWEIEFFKKMQVTEKEDP FT SIAGMVHHKIHDFRWTALGMLPHEDPQPRYLRPVSTAATLNFAATLAQSARLWKDYDPT FT FAADCLEKAEIAWQAALKHPDIYAEYTPGSGGPGGGPYNDDYVGDEFYWAACELYVTTG FT KDEYKNYLMNSPHYLEMPAKMGENGGANGEDNGLWGCFTWGTTQGLGTITLALVENGLP FT ATDIQKARNNIAKAADRWLENIEEQGYRLPIKRAEDERAGYPWGSNSLHFEPDDLVMGY FT AYDFTGDSNISMECLTGISYLLGRNAMDQSYVTGYGERPLQNPHDRFWTPQTSKRFPAP FT PPGIISGRPNSRFEDPTINAAVKKDTPPQKCFIDHTDSWSTNEITVNWNAPFAWVTAYL FT DEQYTDSETDKVTIDSPVAGERFEAGKDINIRTVKSKTPVSKVEFYNGDTLISSDTTAP FT YTAKITGAAVGAYNLKAVAVLSDGRRIESPVTPVLVKVIVKPTVKLTAPKSNVVAYGNE FT FLKITATASDSDGKISRVDFLVDGEVIGSDREAPYEYEWKAVEGNHEISVIAYDDDDAA FT STPDSVKIFVKQARDVKVQYLCENTQTSTQEIKGKFNIVNTGNRDYSLKDIVLRYYFTK FT EHNSQLQFICYYTPIGSGNLIPSFGGSGDEHYLQLEFKDVKLPAGGQTGEIQFVIRYAD FT NSFHDQSNDYSFDPTIKAFQDYGKVTLYKNGELVWGTPPGGTEPEEPEEPEEPEEPAIV FT YGDCNDDGKVNSTDVAVMKRYLKKENVNINLDNADVNADGKVNSTDFSILKRYVMKNIE FT ELPYR" FT RBS 428..432 FT /gene="cbhA" FT terminator 4154..4177 FT /gene="cbh3" XX SQ Sequence 4183 BP; 1311 A; 786 C; 987 G; 1099 T; 0 other; ttgaaaaata cttgacatta ctggttgaca ttttatatat ttaaagtata aggaacataa 60 tttgcaccga tcctgttcta aattctgaaa ttaacaagta tttggggttg tattatatat 120 ggggttgtat cattggggta ttataatcaa tgcaatctat tagtaaaagt ctatgctgtc 180 atatccatca aatctttaaa gcaggtgtct tttgtaacaa attcaataaa cacccattaa 240 ctgtttgtgc gctatgggct atattagatt gaagcttctc acatggcata tttcaaacta 300 ataatataaa accctcaagg aggtgaactc ataagaaggg ttgtaaacct tttttaatta 360 gtttttaatt agtttgaaat gcaaagaaat gagaaagctt caatcctgat ttagcaattc 420 tttaacggga ggcaaagaat gaaatttaga aggtcaattt gtactgctgt tttgttggcg 480 gttttattga cacttctggt accgacatcc gtgtttgcct tagaagataa ttcttcgact 540 ttgccgccgt ataaaaacga ccttttgtat gagaggactt ttgatgaggg actttgttat 600 ccatggcata cctgtgaaga cagcggagga aaatgctcct ttgatgtggt cgatgttccg 660 gggcagcccg gtaataaagc atttgccgtt actgttcttg acaaagggca aaacagatgg 720 agagttcaga tgagacaccg tggtcttact cttgaacagg gacatacata tagagtacgg 780 cttaagattt gggcagatgc gtcctgtaaa gtttatataa aaataggaca aatggcggag 840 ccctatgctg aatattggaa caacaagtgg agtccataca cactgacagc aggtaaggta 900 ttggaaattg acgagacgtt tgttatggac aagccaactg acgacacatg cgaatttaca 960 ttccatttag gtggcgaatt ggcagcaact cctccatata cagtttatct tgatgatgta 1020 tccctttatg acccagaata tacgaagcct gttgaatata tacttccgca gcctgatgta 1080 cgtgtgaacc aggttggcta cctgccggag ggcaagaaag ttgccactgt ggtatgcaat 1140 tcaactcagc cggtaaaatg gcagcttaag aatgctgcag gcgttgtagt tttggaaggt 1200 tataccgaac caaagggtct tgacaaagac tcgcaggatt atgtacattg gcttgatttt 1260 tccgattttg caaccgaagg aattggttac tattttgaac ttccgactgt aaacagtcct 1320 acaaactaca gtcatccatt tgacattcgc aaagacatct atactcagat gaaatatgat 1380 gcattggcat tcttctatca caagagaagc ggtattccta ttgaaatgcc gtatgcagga 1440 ggagaacagt ggaccagacc tgcaggacat atcggaattg agccgaacaa gggagataca 1500 aatgttccta catggcctca ggatgatgag tatgcaggaa tacctcagaa gaattataca 1560 aaggatgtaa ccggtggatg gtatgatgcc ggtgaccacg gtaaatatgt tgtaaacggc 1620 ggtatagccg tctggacatt aatgaacatg tatgagaggg caaaaattag aggtcttgac 1680 aactggggac catacaggga cggcggaatg aacataccgg agcagaataa cggttatccg 1740 gacattcttg atgaagcaag atgggaaatt gagttcttta agaaaatgca ggtaactgaa 1800 aaagaggatc cttccatagc cggaatggta caccacaaaa ttcacgactt cagatggact 1860 gctttgggta tgttgcctca cgaagatccc cagccacgtt acttaaggcc ggtaagtacg 1920 gctgcgactt tgaactttgc ggcaactttg gcacaaagtg cacgtctttg gaaagattat 1980 gatccgactt ttgctgctga ctgtttggaa aaggctgaaa tagcatggca ggcggcatta 2040 aagcatcctg atatttatgc tgagtatact cccggtagcg gtggtcccgg aggcggacca 2100 tacaatgacg actatgtcgg agacgaattc tactgggcag cctgcgaact ttatgtaaca 2160 acaggaaaag acgaatataa gaattacctg atgaattcac ctcactatct tgaaatgcct 2220 gcaaagatgg gtgaaaacgg tggagcaaac ggagaagaca acggattgtg gggatgcttc 2280 acctggggaa ctactcaagg attgggaacc attactcttg ctttggttga aaacggattg 2340 cctgctacag acattcaaaa ggcaagaaac aatatagcta aagctgctga cagatggctt 2400 gagaatattg aagagcaagg ttacagactg ccgatcaaac gggcggagga tgagagagcc 2460 ggttatccat ggggttcaaa ctccttgcat tttgaaccag atgacctagt tatgggatat 2520 gcctatgact ttacaggtga ctcaaatatc tcgatggaat gtttgaccgg cataagctac 2580 ctgttgggaa gaaacgcaat ggatcagtcc tatgtaacag ggtatggtga gcgtccgctt 2640 cagaatcctc atgacaggtt ctggacgccg cagacaagta agagattccc tgctccacct 2700 ccgggtataa tttccggccg tccgaactcc cgtttcgagg acccgacaat aaatgcggcc 2760 gttaagaagg atacaccgcc acagaaatgt tttatcgacc atacagactc atggtcaacc 2820 aacgagataa ctgttaactg gaatgctccg tttgcatggg ttacagctta tcttgacgag 2880 cagtacacag acagtgaaac cgataaggta actattgatt cgcctgttgc aggagaaaga 2940 tttgaagcgg gtaaagacat taatataaga actgttaaat caaaaactcc tgtaagcaaa 3000 gtagagtttt acaatggaga tacgcttatt tccagtgaca caactgcacc ttacacagca 3060 aagataacag gagccgctgt cggagcatat aaccttaaag cggttgcagt gctgtctgac 3120 ggaagaagaa ttgagtcacc ggtaactcct gtacttgtta aggtaattgt gaaacctact 3180 gtaaaactta ctgcacccaa gtcaaatgtt gtggcttatg gaaatgagtt cctgaagatt 3240 acagcaacag ccagtgactc tgacggcaaa atctccaggg ttgatttcct tgttgacggt 3300 gaagtaatcg gttcagacag ggaagcacct tatgaatatg agtggaaagc tgtggaaggc 3360 aatcacgaaa taagtgtaat tgcttatgat gatgacgatg cggcttcaac acctgattcc 3420 gtaaaaatat ttgtaaaaca ggcacgggat gtaaaagtac agtatttgtg cgaaaatacg 3480 caaacatcca ctcaggaaat caagggtaaa ttcaatatag ttaacacagg aaacagagat 3540 tattcgctga aagatatagt attaagatac tactttacca aggagcacaa ttcacagctt 3600 cagtttatct gctattatac acccataggc tccggaaatc tcattccgtc ctttggcggc 3660 tcgggtgacg agcattatct gcagctggaa ttcaaagatg tcaagctgcc tgccggcggt 3720 cagactgggg aaatacagtt tgttataaga tatgcagata actccttcca tgatcagtcg 3780 aacgactatt cgttcgatcc aactataaaa gcgttccagg attatggcaa ggttaccctg 3840 tataagaatg gagaacttgt ttggggaacg ccgccgggcg gtacagaacc tgaagaaccg 3900 gaagagcctg aagaaccgga agagcctgcg atagtttacg gcgactgtaa tgatgacggc 3960 aaagtaaatt caacagacgt cgcagtaatg aagagatatt taaagaaaga aaatgttaat 4020 attaatcttg acaatgcaga tgtgaatgcg gacggcaaag ttaactcaac agacttctca 4080 atacttaaga gatatgttat gaagaacata gaagaattgc catatcgata agataatctg 4140 aaattatttg tgtaaggacc ggtttttgcc ggtccttttt aaa 4183 // |
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