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EBI DbfetchID X80800; SV 1; linear; genomic DNA; STD; PRO; 3239 BP. XX AC X80800; XX DT 20-JUN-1995 (Rel. 44, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 9) XX DE A.calcoaceticus genes lipB, lipA and trmD, partial XX KW chaperone; lipA gene; lipase; lipB gene; methyltransferase; trmD gene. XX OS Acinetobacter calcoaceticus OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Moraxellaceae; Acinetobacter. XX RN [1] RP 1182-2685 RX DOI; 10.1111/j.1365-2958.1995.tb02351.x RX PUBMED; 7596283. RA Kok R.G., Thor J.J., Nugteren-Roodzant I.M., Brouwer M.B.W., Egmond M.R., RA Nudel C.B., Vosman B., Hellingwerf K.J.; RT "Characterization of the extracellular lipase, LipA, of Acinetobacter RT calcoaceticus BD413 and sequence analysis of the cloned structural gene"; RL Mol. Microbiol. 15(5):803-818(1995). XX RN [2] RP 1-3239 RA Hellingwerf K.J.; RT ; RL Submitted (02-AUG-1994) to the EMBL/GenBank/DDBJ databases. RL K.J. Hellingwerf, Dept of Microbiology, E.C. Slater Institute, BioCentrum RL Amsterdam, Nieuwe Achtergracht 127, 1018 WS Amsterdam, NETHERLANDS XX RN [3] RP 1-1181, 2686-3239 RX PUBMED; 7768830. RA Kok R.G., Thor J.J., Nugteren-Roodzant I.M., Vosman B., Hellingwerf K.J.; RT "Characterization of lipase-deficient mutants of Acinetobacter RT calcoaceticus BD413: identification of a periplasmic lipase chaperone RT essential for the production of extracellular lipase"; RL J. Bacteriol. 177(11):3295-3307(1995). XX FH Key Location/Qualifiers FH FT source 1..3239 FT /organism="Acinetobacter calcoaceticus" FT /strain="BD413" FT /mol_type="genomic DNA" FT /clone="mpALJA2 and mpALJA2b" FT /db_xref="taxon:471" FT CDS 172..1203 FT /transl_table=11 FT /gene="lipB" FT /product="lipase foldase" FT /db_xref="GOA:Q43961" FT /db_xref="InterPro:IPR004961" FT /db_xref="UniProtKB/Swiss-Prot:Q43961" FT /protein_id="CAA56779.1" FT /translation="MNGSKKIYLGIGLVALLMIFIYWLMPKDTANASSQIESTNASAII FT ATSPGQQNQLSENTTPFGSVSQHDTQVNCQLQLNAANHLIVNEQTRNCFEYFLTQYGEK FT SLTQIDQDIKNYFTQSLPQPARDQAQDLWQRYLKYREELGNLKEPAIAKTDIAYYRAVF FT TSRQMLRQRFFSATEIAGLFGSEDIYNQYTLERMAILNNSKLSEIEKAKQLKALFDQLP FT QDWKANLEQLSKLDDLKQLTTSIKKNGGSAQELHDMRTNLVGHDATARLEQLDVERSNW FT KSNVTQYLDERQTILNSNMSDTAKQNAISALRSKNFTAPQVQIRVQAFESAKDQGQSLP FT FSE" FT CDS 1335..2306 FT /transl_table=11 FT /gene="lipA" FT /product="lipase" FT /db_xref="HSSP:1EX9" FT /db_xref="InterPro:IPR000073" FT /db_xref="UniProtKB/TrEMBL:Q43962" FT /protein_id="CAA56780.1" FT /translation="MKFKLLFTTLLLVLTQPVFATSPIQNPTTSFVISDYAKTKYPIVL FT SHGLFGFNKLGTEAFGLDYWYQIPQDLARNGANVWVTRQSTANTSEFRGEQLLAEVQDI FT LAITGAQKVNLIGHSHGSQTVRYVAGVLPANIASVSTIGGPAKGAPLADLIYKTLAGTP FT LEAPIATIFNVAMNFITIGQFDDPQKYPMNSVGAAYSLSTEGAGKFNAIFPAGVPTTAC FT GQGESSVNGVRYYSWSGASPLTNPLDPSDYGLSLTSVFSGKNNDGLVPSCSSHLGTVIR FT DNYVWNHLDEVNQILGFDLFLHKTPYPSLDNMPIVSKVKIYN" FT CDS complement(2882..>3239) FT /codon_start=2 FT /transl_table=11 FT /gene="trmD" FT /product="guanine-7-methyltransferase" FT /db_xref="GOA:Q43963" FT /db_xref="InterPro:IPR002649" FT /db_xref="InterPro:IPR016009" FT /db_xref="UniProtKB/Swiss-Prot:Q43963" FT /protein_id="CAA56781.1" FT /translation="DQEWSIGDYVLSGGELPAMVLLDSIIRRLPDAMSDEQSHIQDSFV FT DGLLDCPQYTKPDHFEGLDVPEVLKSGHHANIEKWRFLQRYQRTLDRRPELVEKVTLTK FT QQRKWLTFLDDSKN" FT RBS 159..162 FT /gene="lipB" FT RBS 1326..1329 FT /gene="lipA" FT promoter 84..114 FT /gene="lipB" FT repeat_region 1281..1306 FT /rpt_type=INVERTED FT terminator 2320..2350 FT /note="inverted repeat" XX SQ Sequence 3239 BP; 1048 A; 681 C; 590 G; 920 T; 0 other; gatatcttgc tggattttgc agtcataatg tgctctacat caaataaatt aaatataatg 60 ttaaaaaatt cattcatccg tttttgtatt atttgtccaa agttatgcta tttttataat 120 cattcaattc aatgatgaaa taaaaattgc gtcgtattgg aatataaaaa catgaatgga 180 tcgaaaaaaa tttacctagg gattgggtta gtcgcattat taatgatatt catctattgg 240 ttaatgccaa aagatactgc caatgcatcc tctcaaattg agagtacgaa tgcctcagca 300 attatagcaa ccagtccagg gcagcagaat caactttctg aaaatacgac accatttggt 360 agtgtaagtc agcatgatac tcaagtgaat tgccaacttc agctcaatgc agctaaccac 420 ttaatcgtca atgaacaaac acgtaactgc tttgaatatt ttcttacgca atacggcgag 480 aaaagcctga ctcaaattga tcaagacatc aaaaattact tcactcaaag tcttccccag 540 cctgcacgcg atcaggcaca agatttatgg caacgttatc tcaaatatcg cgaagaactc 600 ggaaatttaa aagaaccagc gatcgccaaa actgatattg cttattatcg tgcagtattt 660 acctctagac agatgctcag acaacgtttt ttttctgcga cagaaattgc agggctgttc 720 ggctcagaag atatttataa ccaatatacc ctggaacgga tggcgattct aaataatagc 780 aaactcagtg agattgaaaa agccaagcaa ctcaaagcat tatttgatca attgccccaa 840 gactggaaag cgaatcttga acaactttca aaattggatg atttaaagca attaaccaca 900 tcaatcaaaa agaatggagg ctcagcacaa gagttacatg atatgcgaac caatttggtt 960 ggacatgatg ccactgcacg tctagaacaa ttggatgtcg aacgctcaaa ctggaaaagt 1020 aacgtcactc agtatcttga tgagcgtcag actattttaa acagcaatat gagtgataca 1080 gcgaaacaga acgccatcag tgcacttaga agtaaaaatt ttactgcacc acaggtacag 1140 attcgggtac aagcatttga atctgcaaaa gatcaaggac aaagcttacc cttttctgaa 1200 taattttata tcactattta atcaaggaga aaatagacct taatttagct ttacccgtgt 1260 tttaacggaa ataatatgcg gtttgaaaat ggatgaattt tcaaactcag aaaaatgaaa 1320 tttagggaaa taaaatgaaa ttcaaattat tatttacaac tttattgctg gttctaaccc 1380 aacctgtatt tgcaaccagt ccaattcaga atccaactac atcttttgtc atatccgatt 1440 atgcaaagac caaatatccg attgttctct cacatggact attcggtttt aataaattag 1500 gcacagaagc ctttgggtta gattattggt atcagattcc acaagatttg gccagaaatg 1560 gtgccaatgt ctgggtaacc cgtcaatcta ctgccaatac atctgaattt cgaggcgaac 1620 aattacttgc tgaagttcag gacattctgg ccattacagg cgcacaaaaa gttaatctga 1680 ttgggcatag tcatggctcc caaactgtac gctatgttgc tggtgttctc cctgcaaata 1740 ttgcttccgt ttcaacgatt ggtggccctg ccaaaggtgc cccactggct gatctgattt 1800 ataaaacact ggctggcaca ccattagaag cgcctattgc gaccattttc aatgtcgcaa 1860 tgaattttat tacgatagga caattcgatg atccgcagaa atatccaatg aactctgtcg 1920 gtgcagcgta tagtctatcc actgaaggtg ctggtaaatt taatgcaatc tttcctgctg 1980 gcgtaccaac gactgcatgc ggtcaaggtg aatcttctgt caatggtgta cgttattatt 2040 catggagcgg tgcttctcca ttaaccaatc cactcgatcc ttcagattat ggcctgagct 2100 tgaccagtgt atttagtggc aaaaacaatg acgggctggt accttcatgt agcagtcact 2160 tgggtacagt aattcgagat aattacgtat ggaatcatct ggatgaagtc aatcaaattc 2220 tgggtttcga tctatttttg cacaagaccc cgtatccatc tttagacaac atgccaatcg 2280 tctcaaaggt caaaatctat aattaagatg caccagatgt aaaaaatgcg cccaatcagg 2340 cgcattttta tgctgataag cgaagttaaa attaacctcg tttacgcgct ggcaattttt 2400 cacgaatacg cgcagcttta ccagacaact cacgaaggta gtaaagttta gcacgacgca 2460 cgtcaccacg acgtttcact tcaattttag caacaactgg tgagtgagtt ggaaacacgc 2520 tcaacaccaa cacagctaga aatttacgta ctgtaaacgc agagtttaaa ccacgatttt 2580 cttcgcaata acaacacctt caaatgcttg aagacgctca cgatcaccct cttttacttt 2640 aacttggaca atcactgtgt caccaggagc aaaagcaggg atatcagatt ttaactgtgc 2700 attttctaca gcttgaacta aaggatgttt accactcatt gtggaatctc cagtatgtgc 2760 gggagagaag aggtctctta caccgctggg gacagaggct aaaacgcata tcaacccgtt 2820 taactttccc tctattactt atactcaagg tataaaaata aagagccata tatcagatca 2880 ctcaattttt tgagtcatct aaaaatgtta gccattttct ttgctgcttg gtcaacgtca 2940 ctttttcaac cagttcaggt cgacgatcta aagtacgttg ataccgctgc aaaaaacgcc 3000 atttttcaat attggcatga tgtcctgatt ttagaacttc aggaacatcc aacccttcaa 3060 aatggtcagg cttggtatat tgtggacaat ctaaaagacc atccacaaaa gaatcttgta 3120 tatgagattg ctcgtcagac atcgcatctg gtaaccgccg aataatacta tccagtaaca 3180 ccatcgcagg aagctcacct cccgataata cataatctcc aattgaccat tcctgatca 3239 // |
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