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EBI DbfetchID X79899; SV 1; linear; genomic DNA; STD; PRO; 1848 BP. XX AC X79899; XX DT 01-JUL-1994 (Rel. 40, Created) DT 23-OCT-2008 (Rel. 97, Last updated, Version 13) XX DE C.difficile genes ech, hbd, thi XX KW beta-hydroxybutyrate dehydrogenase; ech gene; enoyl-CoA hydratase; KW hbd gene; thi gene; thiolase. XX OS Clostridium difficile OC Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; OC Clostridium. XX RN [1] RX DOI; 10.1016/0378-1097(94)90333-6 RX PUBMED; 8001771. RA Mullany P., Clayton C.L., Pallen M.J., Slone R., Al-Salem A., RA Tabaqchali S.; RT "Genes encoding homologues of three consecutive enzymes in the RT butyrate/butanol-producing pathway of Clostridium acetobutylicum are RT clustered on the Clostridium difficile chromosome"; RL FEMS Microbiol. Lett. 124(1):61-67(1994). XX RN [2] RP 1-1848 RA Mullany P.; RT ; RL Submitted (29-APR-1994) to the EMBL/GenBank/DDBJ databases. RL P. Mullany, Dept of Medical Micro., St Bartholowmew Hospital, Medical RL College, West Smithfield, London EC1A 7BE, UK XX FH Key Location/Qualifiers FH FT source 1..1848 FT /organism="Clostridium difficile" FT /strain="E" FT /mol_type="genomic DNA" FT /db_xref="taxon:1496" FT mat_peptide 1..468 FT /gene="ech" FT /product="enoyl-CoA hydratase" FT /EC_number="4.2.1.17" FT CDS 596..1441 FT /transl_table=11 FT /gene="hbd" FT /product="beta-hydroxybutyrate dehydrogenase" FT /EC_number="1.1.1.35" FT /db_xref="GOA:P45364" FT /db_xref="InterPro:IPR006108" FT /db_xref="InterPro:IPR006176" FT /db_xref="InterPro:IPR006180" FT /db_xref="InterPro:IPR008927" FT /db_xref="InterPro:IPR013328" FT /db_xref="InterPro:IPR016040" FT /db_xref="UniProtKB/Swiss-Prot:P45364" FT /protein_id="CAA56272.1" FT /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLDK FT NLTKLVTKGKWMKATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLDEL FT CKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVTFDT FT VFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLGANHP FT MGPLALGDLIGLDVVLAIMNVLYTEFGDTKYTAHPLLAKMVRANQLGRKTKIGFYDYNK FT " FT CDS 1511..>1848 FT /transl_table=11 FT /gene="thi" FT /product="thiolase" FT /EC_number="2.3.1.9" FT /db_xref="GOA:P45362" FT /db_xref="InterPro:IPR002155" FT /db_xref="InterPro:IPR016038" FT /db_xref="InterPro:IPR016039" FT /db_xref="InterPro:IPR020616" FT /db_xref="UniProtKB/Swiss-Prot:P45362" FT /protein_id="CAA56273.1" FT /translation="MGVMNMREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKR FT ANITPDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSWRALR FT TRTGPRRRA" FT RBS 583..587 FT /note="putative" FT repeat_region 509..571 FT /rpt_type=INVERTED FT /note="putative transcription terminator" FT CDS <1..468 FT /codon_start=1 FT /transl_table=11 FT /gene="ech" FT /product="enoyl-CoA hydratase" FT /EC_number="4.2.1.17" FT /db_xref="GOA:P45361" FT /db_xref="InterPro:IPR001753" FT /db_xref="UniProtKB/Swiss-Prot:P45361" FT /protein_id="CAA56274.1" FT /translation="NSKKVVIAAVNGFALGGCELAMACDIRIASAKAKFGQPEVTLGIT FT PGYGGTQRLTRLVGMAKAKELIFTGQVIKADEAEKIGLVNRVVEPDILIEEVEKLAKII FT AKNAQLAVRYSKEAIQLGAQTDINTGIDIESNLFGLCFSTKDQKEGIVSFR" XX SQ Sequence 1848 BP; 713 A; 214 C; 378 G; 543 T; 0 other; aatagtaaaa aagtagtgat agctgctgta aacggatttg ctttaggtgg atgtgaactt 60 gcaatggcat gtgatataag aattgcatct gctaaagcta aatttggtca gccagaagta 120 actcttggaa taactccagg atatggagga actcaaaggc ttacaagatt ggttggaatg 180 gcaaaagcaa aagaattaat ctttacaggt caagttataa aagctgatga agctgaaaaa 240 atagggctag taaatagagt cgttgagcca gacattttaa tagaagaagt tgagaaatta 300 gctaagataa tagctaaaaa tgctcagctt gcagttagat actctaaaga agcaatacaa 360 cttggtgctc aaactgatat aaatactgga atagatatag aatctaattt atttggtctt 420 tgtttttcaa ctaaagacca aaaagaagga attgtcagct ttcgttgaaa agagagaagc 480 taactttata aaagggtaaa aatctaatta aaatatattt gctgttaact agattaaata 540 gtattttaat ctagttaaca gaatataaat actaaaataa caggaggtta taactatgaa 600 attagctgta ataggtagtg gaactatggg aagtggtatt gtacaaactt ttgcaagttg 660 tggacatgat gtatgtttaa agagtagaac tcaaggtgct atagataaat gtttagcttt 720 attagataaa aatttaacta agttagttac taagggaaaa tggatgaaag ctacaaaagc 780 agaaatatta agtcatgtta gttcaactac taactatgaa gatttaaaag atatggattt 840 aataatagaa gcatctgtag aagacatgaa tataaagaaa gatgttttca agttactaga 900 tgaattatgt aaagaagata ctatcttggc aacaaatact tcatcattat ctataacaga 960 aatagcttct tctactaaga gaccagataa agttatagga atgcatttct ttaatccagt 1020 tcctatgatg aaattagttg aagttataag tggtcagtta acatcaaaag ttacttttga 1080 tacagtattt gaattatcta agagtatcaa taaagtacca gtagatgtat ctgaatctcc 1140 tggatttgta gtaaatagaa tacttatacc tatgataaat gaagctgttg gtatatatgc 1200 agatggtgtt gcaagtaaag aagaaataga tgaagctatg aaattaggag caaaccatcc 1260 aatgggacca ctagcattag gtgatttaat tggattagat gttgttttag ctataatgaa 1320 cgttttatat actgagtttg gagatactaa atatacagct catccacttt tagctaaaat 1380 ggttagagct aatcaattag gaagaaaaac taagatagga ttctatgatt ataataaata 1440 agaatttggg aattaaaagt ttaaataaaa ttgtttaaaa acaatttcga tatatgaaaa 1500 atctaattta atgggggtaa tgaatatgag agaagtagta attgccagtg cagctagaac 1560 agcagtagga agttttggag gagcatttaa atcagtttca gcggtagagt taggggtaac 1620 agcagctaaa gaagctataa aaagagctaa cataactcca gatatgatag atgaatctct 1680 tttaggggga gtacttacag caggtcttgg acaaaatata gcaagacaaa tagcattagg 1740 agcaggaata ccagtagaaa aaccagctat gactataaat atagtttgtg gttctggatt 1800 aagatcgtgg cgggcactcc gaacgcgaac gggtccgcgt cgacgtgc 1848 // |
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