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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   X79068; SV 1; linear; genomic DNA; STD; PRO; 1101 BP.
XX
AC   X79068;
XX
DT   05-MAY-1994 (Rel. 39, Created)
DT   18-APR-2005 (Rel. 83, Last updated, Version 3)
XX
DE   T.intermedius (IFO 14230) leucine dehydrogenase gene.
XX
KW   leucine dehydrogenase.
XX
OS   Thermoactinomyces intermedius
OC   Bacteria; Firmicutes; Bacillales; Thermoactinomycetaceae;
OC   Thermoactinomyces.
XX
RN   [1]
RA   Ohshima T., Bakthavatsalam N.N.S., Kataoka K., Takada H., Yoshimura T.,
RA   Esaki N., Soda K.;
RT   "The purification, characterization, cloning and sequencing of the gene for
RT   a halostable and thermostable leucine dehydrogenase from Thermoactinomyces
RT   intermedius";
RL   Eur. J. Biochem. 272:305-312(1994).
XX
RN   [2]
RP   1-1101
RA   Ohshima T.;
RT   ;
RL   Submitted (02-MAY-1994) to the EMBL/GenBank/DDBJ databases.
RL   T. Ohshima, Kyoto  University of Education, Dept of Chemistry, Fushimi-ku
RL   Fujinomoricho, Fukakusa, Kyoto 612, JAPAN
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1101
FT                   /organism="Thermoactinomyces intermedius"
FT                   /strain="IFO 14230"
FT                   /mol_type="genomic DNA"
FT                   /clone_lib="hyblid plasmid (pULDH1, 6.5kb)"
FT                   /sub_clone="(pULDH2, 3.8kb)"
FT                   /cell_line="E.coli JM 109"
FT                   /db_xref="taxon:2024"
FT   CDS             1..1101
FT                   /transl_table=11
FT                   /gene="leudh"
FT                   /product="leucine dehydrogenase"
FT                   /EC_number="1.4.1.9"
FT                   /db_xref="GOA:Q60030"
FT                   /db_xref="InterPro:IPR006095"
FT                   /db_xref="InterPro:IPR006096"
FT                   /db_xref="InterPro:IPR006097"
FT                   /db_xref="InterPro:IPR016040"
FT                   /db_xref="InterPro:IPR016211"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q60030"
FT                   /protein_id="CAA55671.1"
FT                   /translation="MKIFDYMEKYDYEQLVMCQDKESGLKAIICIHVTTLGPALGGMRM
FT                   WTYASEEEAIEDALRLGRGMTYKNAAAGLNLGGGKTVIIGDPRKDKNEAMFRALGRFIQ
FT                   GLNGRYITAEDVGTTVEDMDIIHEETRYVTGVSPAFGSSGNPSPVTAYGVYRGMKAAAK
FT                   EAFGDDSLEGKVVAVQGVGHVAYELCKHLHNEGAKLIVTDINKENADRAVQEFGAEFVH
FT                   PDKIYDVECDIFAPCALGAIINDETIERLKCKVVAGSANNQLKEERHGKMLEEKGIVYA
FT                   PDYVINAGGVINVADELLGYNRERAMKKVEGIYDKILKVFEIAKRDGIPSYLAADRMAE
FT                   ERIEMMRKTRSTFLQDQRNLINFNNK"
FT   misc_feature    522..620
FT                   /note="coenzyme binding domain"
FT   misc_feature    171..281
FT                   /note="substrate binding domain"
XX
SQ   Sequence 1101 BP; 332 A; 232 C; 300 G; 237 T; 0 other;
     atgaaaatct tcgattacat ggaaaaatat gattatgaac aactcgtcat gtgccaagat        60
     aaagagtccg gcctcaaagc catcatttgt atccatgtta ccaccctggg gccggctctg       120
     ggtggtatgc gcatgtggac ttacgcttcg gaagaagaag ccatcgagga tgcgctgcgc       180
     ctgggaagag ggatgactta caaaaatgca gctgccggct taaacctcgg cggcggaaaa       240
     acggtgatca tcggcgatcc gagaaaagac aaaaacgaag ccatgttccg tgctttggga       300
     cgctttatcc aaggattgaa cggacggtac atcacggcgg aagacgtggg aaccactgtg       360
     gaagacatgg atatcattca tgaggaaacc cgttacgtga caggcgtatc gcccgctttc       420
     ggttccagcg gcaacccttc accggtgacg gcttacggcg tataccgtgg aatgaaagcg       480
     gctgcaaaag aagctttcgg cgatgactca ctcgaaggca aagtggtggc tgttcaagga       540
     gtgggacatg ttgcttacga attatgtaag cacttgcata atgaaggggc caaattgatt       600
     gtgaccgaca tcaacaaaga aaatgcagac cgtgccgtgc aagaatttgg tgccgaattt       660
     gttcatccgg acaaaatcta tgatgtggaa tgcgacattt ttgctccttg tgcgctcggg       720
     gcgatcatta atgacgaaac cattgaacgc ttgaaatgca aagtggtggc cggttcggca       780
     aacaatcagc tgaaagaaga gcgccatgga aaaatgcttg aagaaaaagg aatcgtctat       840
     gcgccggatt atgtgatcaa tgccggaggc gtgatcaatg tcgccgatga attgctgggt       900
     tataaccgtg agcgtgcgat gaaaaaagtg gaaggcattt acgacaaaat cctgaaagtg       960
     tttgagatcg caaaacggga cggcattccg agctatctcg cagcagaccg catggcggaa      1020
     gaacggattg aaatgatgcg caaaacaaga agcacatttt tgcaagatca acgcaacctg      1080
     atcaatttta acaacaaata a                                                1101
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