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EBI DbfetchID X79027; SV 1; linear; genomic DNA; STD; PRO; 4972 BP. XX AC X79027; XX DT 12-SEP-1995 (Rel. 45, Created) DT 20-NOV-1996 (Rel. 50, Last updated, Version 8) XX DE M.ammoniaphilum genes mamIR and mamIM XX KW mamIM gene; mamIR gene; methyltransferase; restriction endonuclease. XX OS Microbacterium ammoniaphilum OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; OC Micrococcineae; Microbacteriaceae; Microbacterium. XX RN [1] RX DOI; 10.1016/0378-1119(96)00189-8 RX PUBMED; 8654988. RA Striebel H.M., Seeber S., Jarsch M., Kessler C.; RT "Cloning and characterization of the MamI restriction-modification system RT from Microbacterium ammoniaphilum in Escherichia coli"; RL Gene 172(1):41-46(1996). XX RN [2] RP 1-4972 RA Seeber S.; RT ; RL Submitted (27-APR-1994) to the EMBL/GenBank/DDBJ databases. RL S. Seeber,Boehringer Mannheim Therapeutics, Nonnenwald 2, 82377 Penzberg, RL FRG XX FH Key Location/Qualifiers FH FT source 1..4972 FT /organism="Microbacterium ammoniaphilum" FT /strain="ATCC 15354" FT /mol_type="genomic DNA" FT /db_xref="taxon:42460" FT CDS 191..1279 FT /transl_table=11 FT /gene="mamIM" FT /product="methyltransferase" FT /db_xref="GOA:P50190" FT /db_xref="InterPro:IPR002052" FT /db_xref="UniProtKB/Swiss-Prot:P50190" FT /protein_id="CAA55646.1" FT /translation="MRPLRHAVGSSTVTLETDLTLFPEDLHAPLLGSAGNATVDELALA FT ARFDGLHQLLYTRGGVRPTNAAIEEVGKLLLLRLWLSRDDEASVDGVGLRALFDGAVPD FT ESVVEVTKKAFTQVLTVDRMSLRAVDGSSRPLWPYDEPFRLAEPTVLQSALALVNEILG FT GGTRVADPLGTAFDAFLSGRYDHSGGLGTYLTPSSVARMMAEVVLDLLSSDALADVRAP FT IIADPFCGTGRFLVAAFDAAEERHENVDLAGLLDGGLVGADQSTTAIAKSGLNLLLYGA FT QQPEVYAVADSMTDPGLDRLRGTLAAVLTNPPFGGGKYDDALGIDRTRELFPSVRPNRP FT MDPRLLDSRCLSNFCDPGESLG" FT CDS 1948..2223 FT /transl_table=11 FT /gene="orfC" FT /function="necessary for expression control of mamIM gene" FT /db_xref="UniProtKB/TrEMBL:P94908" FT /protein_id="CAA55647.1" FT /translation="MCSGEFGVFKALVDPYALLALLYDPRVQAQLRPLGSGTSSSRRRI FT GPEDVLALIVPFKTPSELAEIGEQARREVRNIESSRLRLAALHQPG" FT CDS complement(2225..3151) FT /transl_table=11 FT /gene="mamIR" FT /product="restriction endonuclease" FT /db_xref="GOA:P50189" FT /db_xref="InterPro:IPR019067" FT /db_xref="UniProtKB/Swiss-Prot:P50189" FT /protein_id="CAA55648.1" FT /translation="MQNAVSQAISQGIHVRREILGSLTYEQRVFLLEDLFVDLFGHQHV FT MLQRWAALTGQSAQVDTGYIAQFVASIVLGEPGQGFRGKGDDLADGSEVKSAANISGVD FT RPRWNHNLGSLDDDEHRRSRGLPTAGEEYLGVPYMFYLLVDRPHGVSDPAPIRIRAWCI FT DAQEDGDWRDLFETFLTSRRGRTYNFQLHPPVGYDDDVVVNTLGNLDFSNVLVFDARLS FT LADRDRPEIDWHVPLPTQVIPVTGRTRALRYGGRGARPTRLTNTADIVLGTNDLGALFP FT GVLAPRDSYDLATVSEIETEAEVEEYS" FT CDS complement(3382..>4972) FT /codon_start=2 FT /transl_table=11 FT /product="unknown" FT /db_xref="UniProtKB/TrEMBL:P94909" FT /protein_id="CAA55649.1" FT /translation="PAPSATRSPSWSGCSRGIPTSPATTSSTCRTAPAASAPASTAEPH FT AGPAKPGRAEPRGTGTIEPRGSHYRHRRPAQRRQVHPLQRPHQEPGARGELPVRDDRAE FT RGRREPARPAARDARRHLRLGAHPARRGVLRRHRRHRARGERGRGPRQQVPRQHPRGRR FT DRAGRPGLHRRRRRARRRQGRPQVRHGDDQHRADPRRPRDPRAGHHPLREGGQGQEARP FT LRARGGQGGPGGPAGRHPALGDEDRPRARQGARAAHREALHLRLQRRRGRADGCRAQGR FT ARRARRAGGGGLPRREDRVRAHRPRPRGRGGAARLDRAGGVGARPARPRRLRHPRPADL FT PHGGPQGVARLDHPQGLEGAAGSRRHPHRLRARLHQGRGDLLRRPRRDRLGRRGPRQGQ FT GAHGGQGLRHAGRRRRGVPSQLAVLRQGVCQASATLALWMTSGRLLRELAGLLCRDSVE FT SHLDVTTWRADASISRRTWTVMNTRLRQAQKSLAARRPTTSSSISRSCWQTALGNSASR FT RRSRCWSEGATQ" XX SQ Sequence 4972 BP; 845 A; 1593 C; 1608 G; 926 T; 0 other; acgcgctggt cgagcgggcc agtgccaacg tggacgagct gccttccggc tgggagcttg 60 aggctgcgtc caagtctgcc gcgaagcgga gcccggccca ggccagggtt gctgccaagg 120 caccggcacg gaaggctccg gtcaagaagg tggctgcgtg agccgaatgc cagccgtggc 180 gcggcgcacg atgaggcctc tgaggcacgc cgtaggatct tccactgtga ccctcgaaac 240 cgatctgacc ctgttcccag aggatcttca tgctccgctc ttgggttcgg cgggaaacgc 300 gactgtcgac gaactcgcgc tcgcggcccg gttcgatggg cttcaccagc tgttgtacac 360 gcgcggaggc gtacgaccga ccaacgcagc cattgaggag gtcggcaagc tgctactgct 420 tcgcctgtgg ctgagccggg acgacgaggc ctcagtcgat ggagtaggcc ttcgagcact 480 gttcgacgga gcagttccgg acgagtcggt agtggaggtg acaaagaaag ccttcacaca 540 agttcttacg gtcgatcgca tgtctttgcg tgcggtggat ggcagcagcc gtccgctctg 600 gccctacgac gaaccgttcc gcctagcgga accgacggtg ctccagagcg cacttgcact 660 ggtgaacgag attctcggcg gcggaacccg agtcgcggac ccgctaggca cggctttcga 720 cgccttcctt agcggccggt atgaccattc cggtggcctc ggcacttacc tcacgccaag 780 ttccgtcgct cgcatgatgg ctgaggtggt cctcgatctc ctctcgagcg acgccctagc 840 tgacgtgagg gcacccatca tcgcggaccc cttctgcgga actggtcgat ttctcgtggc 900 cgcattcgac gccgcggaag agcgacatga gaacgtggac ttggcgggtc tcctcgatgg 960 aggtctggtg ggggccgatc aatctacgac cgcaattgcg aagtcgggtc tgaacctgct 1020 cctttacggt gcgcaacaac cagaggttta cgcagtcgcc gactcgatga cagatcccgg 1080 gctggaccgg ctaagaggaa ctcttgctgc cgtacttacg aatccgccgt ttgggggcgg 1140 taagtacgac gacgccctgg gcatcgaccg gacccgcgag ctgttcccca gtgtccgtcc 1200 gaatcgaccc atggacccgc gattgctgga ctcgcgctgt ctctcgaact tctgcgaccc 1260 gggggagtcc ttgggatagt tcttccggaa ggcattgtga acgggcgaca gttccaggac 1320 atcctgtcca atccggactt tcacgtcgtc gcttcagtaa gcctccccac tgtcaccttc 1380 gccctcagcg gcactgtggc gaagaccagc gcggtattca ttcgacgggt tccacggggg 1440 gaacggacta ctctcgctcg agttgatcac gtcggcttca ttaagcaggc tgggcgagca 1500 gtcgccgatc ccgagggaag cgatattccc gctgtaactg ccgcgctgct caatacactc 1560 aagtccaatc ccgcgtcgga cgagatcgag acggtgtcct ctcgtccgct cgtggcatct 1620 gtcccgtcgg acgggctcac cagcgtggac ccgaaccgcc tagacccgga agcagtgaag 1680 tcccgccatg agcttctcgc gacgggcgga aatcgagctc ggtgagctgc ttcgcactgc 1740 tcctaggtca aggaagggga agcgcacaga cgagcccttc gtctccgttc ttcacgtgga 1800 tcagctcggc accgtcagtt ggaacgaaac actccaatac aaccccacta ctccaggcca 1860 gtgggcgtca tcgggtgatt tgatcgtctc gctgctcaac cttccaagct gcgggcggcg 1920 gtgatcgcag aggactatcc cacggtgatg tgcagcgggg agttcggcgt gtttaaggct 1980 ttggtagacc cctatgcgct ccttgcactg ctttacgacc cccgcgtcca agcgcagctt 2040 cgaccccttg gcagtggcac atcgagctca cgacgtcgaa ttgggccgga ggacgtgctc 2100 gctctcatcg tgccgttcaa gacgccgtcc gagctagccg aaatcggcga gcaggcccgc 2160 cgcgaagttc gaaacatcga gtcttcccgg ctacggctcg cagcgctcca ccagcccggc 2220 tgattcagct gtattcctcc acctccgctt ccgtctcgat ctctgagacg gtcgcgaggt 2280 cgtagctgtc ccgcggagcg aggacgccag ggaagagagc gccgagatcg ttggtcccga 2340 ggacaatgtc ggcagtattg gtcaggcgcg tggggcgtgc acccctcccc ccgtaccgga 2400 gcgctcgggt tcgaccggtg actggaatta cctgagtcgg cagcggaaca tgccagtcga 2460 tctcggggcg gtccctgtcc gcaaggctca atcgagcgtc gaaaacgagc acgttcgaga 2520 agtcaagatt cccgagtgtg ttcacaacca cgtcgtcgtc atagccgacc ggggggtgga 2580 gctgaaagtt gtaggtccgc cccctgcgcg aagtaaggaa ggtctcgaag agatcgcgcc 2640 agtctccgtc ctcctgggca tcgatgcacc aagcccggat gcggattggg gccgggtctg 2700 agacgccgtg tgggcggtct accagcagat agaacatgta gggcacgccc aggtattctt 2760 cgccggcagt tggcagcccg cgcgagcggc ggtgctcgtc gtcgtccagg gaccccaaat 2820 tgtgattcca tctcggccga tcaacacccg aaatgtttgc ggcgctcttg acctcgctcc 2880 cgtcggccag gtcgtcgccc tttcctcgga agccctgccc aggctcgccc agcacgatgg 2940 aagcgacgaa ctgggcgatg tagccggtgt ctacctgtgc agactgccca gttagggctg 3000 cccatcgttg gagcatcacg tgctggtggc cgaacaagtc aacaaagaga tcctcaagga 3060 ggaagacgcg ctgttcgtac gtcaggcttc ccaggatctc acggcgtaca tgaatgccct 3120 gactgattgc ctggctgact gcgttctgca tccttcactc ccgacggcgg catggagccg 3180 tgcgatgaac gtatccaaga acgggtgttg agctcaaact cggaagccgc acacggatcg 3240 gcttgaattc gtcatccaga gccgtcctga ttctgttgcc taccaccgtt gatgatcggc 3300 tcgtcagtct ggcgctcgtg ggctgcgagg gcccgattca ccaagcaccg ccgccgctcg 3360 gccactgtcc cttgcgatac ttcactgcgt agcgccctcc gaccaacagc gagatcgtcg 3420 cctcgacgca ctgttcccga gtgcggtctg ccagcaggaa cggctgatcg aactcgaagt 3480 agtaggtctc cgggccgcca gtgacttctg cgcctgacgc agtcgcgtat tcatcaccgt 3540 ccacgtgcgc cgtgaaatac tggcgtccgc ccgccacgtt gtcacgtcga ggtgcgattc 3600 gaccgaatcc cggcatagca atccagccag ttccctcaac aacctgccac tcgtcatcca 3660 tagcgcgagc gtagcggagg cctgacacac accctgtcgc aacaccgcta actgtgacgg 3720 aactccacga cgtcgccgtc ctgcatgacg tagtccttgc cctccatgcg cgccttgccc 3780 ttggcgcggg cctcggcgac cgagccggtc tcgacgaggt cggcgaagga gatcacctcg 3840 gccttgatga agccgcgctc gaagtcggtg tggatgacgc cggctgcctg cggcgccttc 3900 cagcccttgt ggatggtcca ggcgcgcgac tccttggggc ccgccgtgag gtaggtctgc 3960 aggccgaggg tgtcgaagcc gacgcgggcg agctggtcga gccccgactc ctcctgcccg 4020 gtcgaggcga gcagctccgc cgcgtcctcg gggtcgaggt cgatgagctc ggactcgatc 4080 ttcgcgtcga ggaagaccgc ctccgccggc gcgacgagcg cggcgagctc ggccttgcgc 4140 gcggcatccg tcagcacgcc ctcgtcgacg ttgaagacgt agatgaaggg cttcgcggtg 4200 agcagcccga gctccttgac gggctcgagg tcgatcttcg tcgccgagag cggggtgccg 4260 gcctgcaggg cctcctgggc ctccttggcc gccgcgagca cggaggggtc gagcttcttg 4320 cccttgacct ccttctcgta gcgggtgatg gcccgctcga gggtctcgag gtcggcgagg 4380 atcagctcgg tgttgatcgt ctccatgtcg gacttggggt cgaccttgcc gtcgacgtgc 4440 acgacgtcgc cgtcggtgaa gccccggacg acctgcgcga tcgcgtcggc ctcgcggatg 4500 ttggcgagga acttgttgcc gaggccctcg ccctcgctcg ccccgcgcac gatgccggcg 4560 atgtcgacga aggacaccgc ggcgggcagg atgcgctccg agccgaagat gccggcgagc 4620 gtctcgagcc gcgggtcggg caggttcacg acgcccacgt tcggctcgat cgtcgcgaac 4680 gggtagttcg ccgcgagcac ctggttcttg gtgagggcgt tgaagagggt ggacttgccg 4740 acgttgggca ggccgacgat gccgatagtg agagccacgg ggctctatcg taccggtgcc 4800 gcgcggttcc gcccggccgg gcttcgccgg cccggcgtgg ggctcagccg tcgaggcggg 4860 cgcggaagca gcgggtgcgg tacggcacgt cgagctcgtc gtggccggcg aggtcgggat 4920 gccgcgcgaa cagccgctcc agctgggcga tcgtgtcgcg ctgggcgccg gg 4972 // |
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