EBI Dbfetch

ID   X78852; SV 1; linear; mRNA; STD; PLN; 1910 BP.
XX
AC   X78852;
XX
DT   19-APR-1994 (Rel. 39, Created)
DT   19-APR-1994 (Rel. 39, Last updated, Version 1)
XX
DE   R.sativus (cv Saxa Knacker) mRNA for malate synthase
XX
KW   malate synthase; MLS gene.
XX
OS   Raphanus sativus (radish)
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids;
OC   eurosids II; Brassicales; Brassicaceae; Raphanus.
XX
RN   [1]
RA   Vollack K.U., Bach T.J.;
RT   "Overexpression of radish malate synthase in yeast.";
RL   Unpublished.
XX
RN   [2]
RP   1-1910
RA   Vollack K.;
RT   ;
RL   Submitted (08-APR-1994) to the EMBL/GenBank/DDBJ databases.
RL   K. Vollack, Botanisches Institut II, der Universitaet (T.H), Kaiserstr 12,
RL   76128 Karlsruhe, FRG
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1910
FT                   /organism="Raphanus sativus"
FT                   /cultivar="Saxa Knacker"
FT                   /mol_type="mRNA"
FT                   /dev_stage="4 day old seedlings"
FT                   /clone_lib="lambda uniZAP-cRS"
FT                   /clone="cRS1"
FT                   /tissue_type="etiolated sedlings without roots"
FT                   /db_xref="taxon:3726"
FT   CDS             31..1731
FT                   /gene="MLS"
FT                   /product="malate synthase"
FT                   /EC_number="4.1.3.2"
FT                   /db_xref="GOA:Q43827"
FT                   /db_xref="InterPro:IPR019830"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q43827"
FT                   /protein_id="CAA55407.1"
FT                   /translation="MELETSVYRPNAAVYDSPDGVEVRGRYDQVFAKILTREALGFVAE
FT                   LQRDVSLGMLRYAWSAVERQNVVTTFVLSLGLTLPLSSSEIGEWVCSVCSPPAVADRRV
FT                   EITGPVERKMIINALNSGAKVFMADFEDALSPSWENLMKGQVNLKDAVDGTITFHDKAR
FT                   NKVYKLNDQVAKLFVRPRGWHLPEAHILIDGEPAIGCLVDFGLYFFHNYSKFRQTQGSG
FT                   YGPFFYLPKMEHSREAKIWNSVFERAEKMAGIERGSIRATVLIETLPAVFQMNEILYEL
FT                   RDHSVGLNCGRWDYIFSYVKTFQAHPDRLLPDRVLVGMGQHFMRSYSDLLIRTCHKRGV
FT                   HAWEGMAAQIAIRDDPKANDMALDLVKKDKLRQVRAGHDGTWAAHPGLIPICMDAFGHM
FT                   GKNPNQIKSMKRDDASAITEEDLLQIPRGVRTLDGLRLNTRVGIQYLAAWLTGSGSVPL
FT                   YNLTDEDAATAEISRVQNWQWIRYGVELKRRTGLEVRVSKELFGRVVEEEMERIEKEVG
FT                   KERSLREECIRKLARCLQSSVTAAELDDFLTLAVYDHIVAHYPINVSRL"
XX
SQ   Sequence 1910 BP; 527 A; 375 C; 510 G; 498 T; 0 other;
     tctagactag cggtaacaaa ggccaaaaaa atggagctcg agacctcagt ttatcgacca        60
     aacgccgccg tgtatgattc ccccgatggt gtggaggttc gtggacgtta cgaccaagtc       120
     ttcgctaaga ttctgacaag agaagctctt ggttttgtag ctgagctaca gagagatgtt       180
     tcgttgggca tgttgagata cgcatggagt gccgtagaga ggcaaaacgt cgttacaact       240
     tttgtgctgt ccctgggttt gacccttcca ctaagttcat cagagatcgg tgaatgggtt       300
     tgtagcgtct gctcccctcc agctgttgct gaccggagag ttgagataac cggtccggtc       360
     gagaggaaga tgatcatcaa tgctcttaac tctggagcta aagtcttcat ggcagatttt       420
     gaggatgcac tgtctccaag ctgggagaat ctgatgaaag ggcaagtgaa cttgaaagat       480
     gcagtggatg gaaccattac gtttcacgac aaggcgagga acaaagttta caagctcaac       540
     gaccaagttg ctaagctctt tgtcaggcca cgtggttggc atcttccgga ggctcacatc       600
     ctcatcgacg gtgaaccagc cattggttgt ctcgtcgact tcggtcttta cttcttccac       660
     aactactcca agtttagaca gactcaaggt tccggttatg gacccttctt ctatcttccc       720
     aaaatggaac attctaggga agcaaagatc tggaacagtg tgtttgagcg agctgagaag       780
     atggctggta tagagcgagg aagcattaga gccacggtac tgatcgagac cctcccagct       840
     gttttccaga tgaatgagat actctatgaa ctgcgtgatc attcggtggg tctgaactgt       900
     ggaagatggg attatatatt cagctatgta aagaccttcc aggcccaccc tgaccgtctc       960
     ttaccagacc gcgtccttgt cggtatgggc cagcacttca tgaggagtta ctctgatctt      1020
     ctcattcgta cgtgtcataa gcgtggtgtg cacgcatggg aaggcatggc tgctcagatt      1080
     gccataagag atgacccgaa agcgaatgac atggcgttgg acctagtgaa gaaagacaag      1140
     ctaaggcaag taagagcagg acatgatgga acatgggctg ctcatccagg acttatccca      1200
     atctgcatgg atgcattcgg ccacatgggg aaaaatccaa accaaatcaa atcaatgaag      1260
     cgtgatgacg cgtctgctat aacagaggaa gatttgcttc agatacctag aggagtacga      1320
     acactggacg gtctaaggct gaacacacgt gtggggatcc aatacttagc cgcgtggcta      1380
     acaggatcag gatctgtccc actctataac ctgactgatg aagatgcagc tacggccgag      1440
     ataagccgtg tccaaaactg gcagtggatt aggtatggag tggagttgaa acggagaacg      1500
     ggcttggaag ttagggtgag caaagagttg tttgggagag ttgtggaaga agagatggaa      1560
     agaatcgaga aagaagttgg caaggagaga agtttaagag aggaatgtat aaggaagctt      1620
     gcaagatgtt tacaaagcag tgtaactgcc gctgagcttg atgattttct tacacttgct      1680
     gtttatgatc atattgtagc tcattaccca atcaatgtgt caaggctctg aaagaagaaa      1740
     gattaagaaa agaagatgca atggagagag agtttgaggt atgcgttttg tgcttttctg      1800
     ttctgtgttt gtttctcatt ttgctttttt gtgtttctaa gtttctatat gttggtatat      1860
     tgtaataagc agaacttgat gttttatttc caatatgaaa taaactcgag                 1910
//